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Gene information
General information
TC000737 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 1089435..1091492
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000737
    iBeetle screen iB_00164
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection


    Comment: died with yellowish feces at posterior

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000737 RA
      MGKRRKGRC VKNNTNVHS EPAEVVQAP HSFVIHKGL 
      PGGHLLELT KDFRKVMEP FTATSLKER KKNTVKDFV 
      AVAGLLHVS HLMIFSRTE IGMYLKICR LPRGPTMTF 
      KIHNFSLAR DVVSSLKKQ AVVEAAFKH SPLIVLNSF 
      TSEGMHLKL MASMFQNMF PTINLTNVD LNNVRRCVL 
      LNYNPTTKL IDFRHYTIK VIPVGISKG VKKVVQGKV 
      PNLAKCGDI SEFLTKSGM LSESEFEDD PSNHVTLSQ 
      KLISRGNIE SGKSSIKLT ELGPRLTLQ LIKIEDGLL 
      DGEVLYHDL IEKTDEEKL EIQKKREAK RKLKEARKK 
      TQEKNKEKK ENMKKELKE KSLEGMKKK ESNEEVAVE 
      DDNDAEYYK EEVGEEPDK DLFTQSQRK PSRPVQFRK 
      RKSSEKPPN SAKKFKRND GKSTRHLQN RDQRPIGGQ 
      AFGKGKVKN KRNFKK
    CDS sequence
    • >TC000737 RA
      atgggaaaa cgcagaaag gggcgttgt gtgaaaaac 
      aacacgaat gtgcacagc gaaccggcg gaagtggtg 
      caagccccg cattcgttt gtcattcat aaggggctc 
      ccgggtggc cacttgtta gagcttacg aaggatttt 
      cgtaaggtt atggaaccc ttcactgct acttccctt 
      aaagagcgt aagaaaaac acagttaag gattttgtt 
      gccgttgcc ggtttgttg cacgtgtct catttaatg 
      attttttcg cgcaccgaa attgggatg tatctgaaa 
      atctgtcga ttgccgcga gggcctaca atgaccttc 
      aaaatccac aatttttcc cttgctaga gatgtggtt 
      tcatcgctt aaaaaacaa gcagtggtg gaggctgcg 
      tttaaacat tcacctttg attgttttg aacagtttt 
      acaagtgaa gggatgcac cttaaactg atggcttca 
      atgttccaa aatatgttt cctacaatt aatttaaca 
      aatgttgat ttgaacaat gtgagacgt tgtgtgttg 
      ttaaattat aatcctact acgaaatta attgacttt 
      agacattac acaatcaaa gtaattcct gttggaatt 
      tcaaaagga gtgaaaaaa gttgtccaa ggcaaagta 
      ccgaatctg gcaaaatgt ggtgatatt tcagaattt 
      ctaacaaaa tcaggaatg ttatcagag agtgaattt 
      gaggatgat cccagcaat catgtaacg ttatctcag 
      aaacttatt tcaagagga aatattgaa tctgggaaa 
      tcctctatc aaattaaca gaactaggg ccaagacta 
      actttacag ttaattaaa attgaggat ggtctactg 
      gatggcgag gttttgtac cacgattta atcgaaaaa 
      accgacgaa gagaaactg gaaatacaa aagaaacgt 
      gaagctaaa agaaaattg aaagaggcg cggaagaag 
      acacaagag aaaaataag gaaaagaag gagaacatg 
      aaaaaggaa ttaaaagaa aagtcactt gaaggaatg 
      aagaagaaa gaatcaaat gaggaggtt gctgttgaa 
      gatgataat gatgcagaa tactacaag gaagaggtg 
      ggggaagaa cccgataaa gatttgttt acacaatcg 
      caaagaaaa ccctcaaga cctgttcag ttcaggaag 
      aggaaatca agcgaaaag ccgcctaat tccgcaaaa 
      aaattcaaa cgaaacgac ggaaaaagc actagacat 
      ttacaaaat cgtgaccag cgaccaatc ggtggccaa 
      gcctttggg aaagggaag gtaaaaaac aaacgtaat 
      tttaaaaaa tga
    mRNA sequence
    • >TC000737 RA
      gcacaaaaa cgaattcaa ttagtccgc ctggttgaa 
      gttctctca attttaaac cgttggtag ccgtggtgt 
      tgtggttac cacgtgaca aagtaacct caaagagtg 
      ttatttttt ccctaaatt tatcgtaaa aatgggaaa 
      acgcagaaa ggggcgttg tgtgaaaaa caacacgaa 
      tgtgcacag cgaaccggc ggaagtggt gcaagcccc 
      gcattcgtt tgtcattca taaggggct cccgggtgg 
      ccacttgtt agagcttac gaaggattt tcgtaaggt 
      tatggaacc cttcactgc tacttccct taaagagcg 
      taagaaaaa cacagttaa ggattttgt tgccgttgc 
      cggtttgtt gcacgtgtc tcatttaat gattttttc 
      gcgcaccga aattgggat gtatctgaa aatctgtcg 
      attgccgcg agggcctac aatgacctt caaaatcca 
      caatttttc ccttgctag agatgtggt ttcatcgct 
      taaaaaaca agcagtggt ggaggctgc gtttaaaca 
      ttcaccttt gattgtttt gaacagttt tacaagtga 
      agggatgca ccttaaact gatggcttc aatgttcca 
      aaatatgtt tcctacaat taatttaac aaatgttga 
      tttgaacaa tgtgagacg ttgtgtgtt gttaaatta 
      taatcctac tacgaaatt aattgactt tagacatta 
      cacaatcaa agtaattcc tgttggaat ttcaaaagg 
      agtgaaaaa agttgtcca aggcaaagt accgaatct 
      ggcaaaatg tggtgatat ttcagaatt tctaacaaa 
      atcaggaat gttatcaga gagtgaatt tgaggatga 
      tcccagcaa tcatgtaac gttatctca gaaacttat 
      ttcaagagg aaatattga atctgggaa atcctctat 
      caaattaac agaactagg gccaagact aactttaca 
      gttaattaa aattgagga tggtctact ggatggcga 
      ggttttgta ccacgattt aatcgaaaa aaccgacga 
      agagaaact ggaaataca aaagaaacg tgaagctaa 
      aagaaaatt gaaagaggc gcggaagaa gacacaaga 
      gaaaaataa ggaaaagaa ggagaacat gaaaaagga 
      attaaaaga aaagtcact tgaaggaat gaagaagaa 
      agaatcaaa tgaggaggt tgctgttga agatgataa 
      tgatgcaga atactacaa ggaagaggt gggggaaga 
      acccgataa agatttgtt tacacaatc gcaaagaaa 
      accctcaag acctgttca gttcaggaa gaggaaatc 
      aagcgaaaa gccgcctaa ttccgcaaa aaaattcaa 
      acgaaacga cggaaaaag cactagaca tttacaaaa 
      tcgtgacca gcgaccaat cggtggcca agcctttgg 
      gaaagggaa ggtaaaaaa caaacgtaa ttttaaaaa 
      atgattata aatttattt caaattgga ttgtt