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Gene information
General information
TC000726 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6780221..6781733
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC000726
      iBeetle screen iB_00163

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC000726 RA
        MKIFVCFVL VATIALCAA ENKDKTEKS AKKEDKPKR 
HIFGTGYNN YDFSGFDTV GSLGGVTYG SGLGSVYST 
GSGYGSGLA SVYSHGSGY GSGLGSAYS YGSGYGSGY 
GSGYGSGYG YGSGYGYGT GYGSGLAAG VVTTRASTG 
LRAGSGYAA IEGLPKVSN VRSHEIHTV TQHVPVAVP 
QPYPVHITK TVPVPKPYP VAVEKPVPV PYKVNVPVE 
VPKPYPVKV PQPVAVPYE VKVPVEVPK PYPVHITKT 
VNVPVEKPV YVKVAHPVP VKVREPVPV AVPHPVPVK 
VPTPVVVKV PEVVGVNTV THVDSGAGH IIGNDFGSE 
IVHGAGYGY GAGYGYGSG YGTGAEIIS GSGFGSGAV 
LSTGSAGFG TGAVLSTGS AGFGTGAVL SSGYETSGL 
AGEAISSGL SSGAEIIAA KSAHIVSGS NHGLVGSYV 
PSVYGGISG HTVHNTHWK HL
      CDS sequence
      • >TC000726 RA
        atgaagatc tttgtgtgc tttgtcctg gttgccacc 
atcgcacta tgtgctgca gaaaataaa gacaaaact 
gaaaaatct gccaagaaa gaagacaaa ccaaaaagg 
cacatcttc ggtaccggg tacaacaat tacgacttt 
tcgggcttt gacaccgtt ggatcgttg ggtggtgtg 
acttacgga tccggtctt gggtctgtc tacagtact 
ggctccggt tacggatcc ggtcttgcc tctgtctac 
agtcacggc tccggttat ggttccggt cttgggtca 
gcctacagc tatggatcg ggttatgga tctggttat 
ggatctggt tacggatct ggttatgga tatggatcc 
ggttatgga tacggtact ggatatgga tcaggttta 
gcagctggt gttgtcacc accagagcg tcaactgga 
ctccgtgct ggaagtggt tacgctgcc atcgaaggt 
ttacccaaa gtttccaac gtaaggtcc cacgaaatc 
cacactgtt acccaacac gtgccagtg gcagtaccc 
caaccctac cccgtccac atcaccaag acagtacca 
gtgcccaaa ccctacccc gttgcagtt gagaaacca 
gttccagtt ccatacaaa gtcaacgtt cctgttgaa 
gtacccaaa ccctacccc gtcaaagta ccccaaccc 
gtggctgtg ccctacgaa gtcaaagta ccagtagaa 
gtacccaaa ccatacccc gtccacatc accaaaacc 
gtcaacgtt cccgttgaa aaacccgtc tatgttaaa 
gtagctcac ccagttccc gttaaagtt agggaacca 
gttccagta gctgtaccc caccccgtt cccgttaaa 
gtacccacc cctgtggta gtcaaagta cctgaagtc 
gttggtgtc aacaccgta actcacgtc gattccggc 
gctggtcac atcatcgga aacgacttt ggaagtgaa 
attgtccat ggagctggt tatggatat ggagctggt 
tatgggtac ggatccgga tatggaact ggtgccgaa 
atcatcagc ggatcagga ttcggaagc ggtgccgtt 
ttgagcact ggatcagca ggattcgga accggcgcg 
gttttgagc actggatca gcaggattc ggaaccggc 
gcggttttg agctcagga tatgaaact tccggattg 
gccggtgaa gctatcagc tcaggactt tcctctgga 
gccgaaatt attgctgcg aaatccgct catatcgtg 
tccggaagc aaccacgga cttgttgga tcgtatgta 
ccaagtgtc tacggagga atttcaggc cataccgtt 
cataacacc cattggaaa catttgtaa
      mRNA sequence
      • >TC000726 RA
        ggttttgta gctgcttca agcacatca aaatgaaga 
tctttgtgt gctttgtcc tggttgcca ccatcgcac 
tatgtgctg cagaaaata aagacaaaa ctgaaaaat 
ctgccaaga aagaagaca aaccaaaaa ggcacatct 
tcggtaccg ggtacaaca attacgact tttcgggct 
ttgacaccg ttggatcgt tgggtggtg tgacttacg 
gatccggtc ttgggtctg tctacagta ctggctccg 
gttacggat ccggtcttg cctctgtct acagtcacg 
gctccggtt atggttccg gtcttgggt cagcctaca 
gctatggat cgggttatg gatctggtt atggatctg 
gttacggat ctggttatg gatatggat ccggttatg 
gatacggta ctggatatg gatcaggtt tagcagctg 
gtgttgtca ccaccagag cgtcaactg gactccgtg 
ctggaagtg gttacgctg ccatcgaag gtttaccca 
aagtttcca acgtaaggt cccacgaaa tccacactg 
ttacccaac acgtgccag tggcagtac cccaaccct 
accccgtcc acatcacca agacagtac cagtgccca 
aaccctacc ccgttgcag ttgagaaac cagttccag 
ttccataca aagtcaacg ttcctgttg aagtaccca 
aaccctacc ccgtcaaag taccccaac ccgtggctg 
tgccctacg aagtcaaag taccagtag aagtaccca 
aaccatacc ccgtccaca tcaccaaaa ccgtcaacg 
ttcccgttg aaaaacccg tctatgtta aagtagctc 
acccagttc ccgttaaag ttagggaac cagttccag 
tagctgtac cccaccccg ttcccgtta aagtaccca 
cccctgtgg tagtcaaag tacctgaag tcgttggtg 
tcaacaccg taactcacg tcgattccg gcgctggtc 
acatcatcg gaaacgact ttggaagtg aaattgtcc 
atggagctg gttatggat atggagctg gttatgggt 
acggatccg gatatggaa ctggtgccg aaatcatca 
gcggatcag gattcggaa gcggtgccg ttttgagca 
ctggatcag caggattcg gaaccggcg cggttttga 
gcactggat cagcaggat tcggaaccg gcgcggttt 
tgagctcag gatatgaaa cttccggat tggccggtg 
aagctatca gctcaggac tttcctctg gagccgaaa 
ttattgctg cgaaatccg ctcatatcg tgtccggaa 
gcaaccacg gacttgttg gatcgtatg taccaagtg 
tctacggag gaatttcag gccataccg ttcataaca 
cccattgga aacatttgt aaattgtaa ctagtcttg 
atgcaatgt gaaaaatat gtaaaacta tctacgaag 
caataaagt gatgctata ata
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY