Status
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Gene information
General information
TC032080
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000714,TC000715
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 1457667..1476003
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032080
    iBeetle screen iB_00158
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature differentiation incomplete - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032080 RA
      MNQEPLGVA SDVVHPTDL PRWSEIAPA HYLTDYSAS 
      GAPKHNNSP FPYDSELNH KIILWSGDI SALQVDAIV 
      NSTNESMNE SNPVSDRIF QRAGSELKE EINLDIREC 
      RTGEVRVTQ GHALPARYI IHTVGPKYN IKYQSASEN 
      TLHICYRNV LQKSKEMGL HSIALCVIN SVKRNYPPD 
      EGAHIALRT VRRFLEHHG SSFDTVIFT VDNTDLGIY 
      EVLLPLYFP RSKFEEDAA RWQLPSDVG GHDGEPMVP 
      DRQIRIIDN PQHTLHADE ESIDLSSQL ETSINVGEH 
      AFSQMQGDL DQQRLLGER PYNDPLADI MVKEIQHQE 
      RYERLLRRA KTEDLTEVS GIGCLYQSG VDRLGRPVV 
      VFIGKWFPF NKINLDKAL LYLITLLDP IVKGDYVIA 
      YFHTLTSSS NYPSFSWLR EVYNVLPYK YKKNLKAFY 
      IVHPTFWTK MMTWWFTTF MAPAIKQKV HSLPGVEYL 
      YAVMSPDQL EIPAFITEY DMTINGLRY FQPGAPT
    • >TC032080 RB
      MNQEPLGVA SDVVHPTDL PRWSEIAPA HYLTDYSAS 
      GAPKHNNSP FPYDSELNH KIILWSGDI SALQVDAIV 
      NSTNESMNE SNPVSDRIF QRAGSELKE EINLDIREC 
      RTGEVRVTQ GHALPARYI IHTVGPKYN IKYQSASEN 
      TLHICYRNV LQKSKEMGL HSIALCVIN SVKRNYPPD 
      EGAHIALRT VRRFLEHHG SSFDTVIFT VDNTDLGIY 
      EVLLPLYFP RSKFEEDAA RWQLPSDVG GHDGEPMVP 
      DRQIRIIDN PQHTLHADE ESIDLSSQL ETSINVGEH 
      AFSQMQGDL DQQRLLGER PYNDPLADI MVKEIQHQE 
      RGMAVESPY PNVLTSVTK FLGWTSIEI DEDRYERLL 
      RRAKTEDLT EVSGIGCLY QSGVDRLGR PVVVFIGKW 
      FPFNKINLD KALLYLITL LDPIVKGDY VIAYFHTLT 
      SSSNYPSFS WLREVYNVL PYKYKKNLK AFYIVHPTF 
      WTKMMTWWF TTFMAPAIK QKVHSLPGV EYLYAVMSP 
      DQLEIPAFI TEYDMTING LRYFQPGAP T
    CDS sequence
    • >TC032080 RA
      atgaatcag gagccctta ggggtagca tcggacgtg 
      gtgcacccg accgatctg ccgaggtgg agcgaaata 
      gctccagct cattatctg actgattac agtgccagc 
      ggagcaccc aagcacaat aactcaccc ttcccttac 
      gactccgaa ctcaatcac aaaattatc ttatggtct 
      ggtgatatt tccgcactc caagtagat gctatagtc 
      aattccacg aacgaatcg atgaacgaa agtaatccg 
      gtcagtgat agaattttc caaagggcc ggatctgaa 
      cttaaagag gaaatcaat ttagatata agagagtgt 
      aggacggga gaagtcaga gtcacacaa ggacatgca 
      ctgccagct cgatatatt atacacacc gttggaccc 
      aaatataat ataaaatac caatctgct tcggaaaac 
      acattacat atatgttat agaaacgtt ctccagaaa 
      tcaaaagaa atgggtctc cactccatc gccctatgc 
      gtcataaac tccgtgaaa agaaactat cctcccgac 
      gaaggagca cacatagca ttacgtacg gtgaggaga 
      ttcctcgag caccacggt agttctttt gataccgtc 
      atcttcacg gtcgacaat acagattta ggtatttac 
      gaagtgcta ttacctctc tattttcca cgatctaaa 
      ttcgaggag gacgccgct cgttggcag ctaccttca 
      gacgtcgga ggacacgac ggagagccc atggtgccg 
      gacagacaa attcggatt atcgacaat ccacaacac 
      actctgcat gccgacgaa gaatctata gatttgtcg 
      tctcaactg gaaacatct ataaatgtg ggtgaacat 
      gcattcagt caaatgcag ggtgatctc gaccaacag 
      cggcttctg ggggaaaga ccctataat gatccactc 
      gccgatatt atggtcaag gaaatacaa caccaggaa 
      cgatacgag cggctcttg agacgtgcc aaaaccgaa 
      gacctgacg gaggtctcc ggcattggc tgtctgtac 
      cagagcgga gtggaccga ctaggtcgg ccagtggtg 
      gtattcatc ggaaaatgg tttcctttc aacaaaatt 
      aatttagat aaggcttta ttgtatcta ataacgttg 
      ctggatccg atcgtgaag ggcgactac gttatcgcc 
      tacttccat acgcttacc tccagcagc aactatccg 
      tcgttctca tggctgaga gaggtctat aatgtgctg 
      ccttacaag tataagaag aatcttaag gctttctac 
      atcgtgcat ccgacgttt tggacgaag atgatgact 
      tggtggttt accacgttt atggcaccg gccattaag 
      caaaaagtg cacagttta cccggagtg gagtacctc 
      tatgcggtg atgtcgccc gatcagttg gaaattccc 
      gcatttatt accgaatat gatatgact ataaacggc 
      ctgaggtat tttcagccc ggagcgccg acataa
    • >TC032080 RB
      atgaatcag gagccctta ggggtagca tcggacgtg 
      gtgcacccg accgatctg ccgaggtgg agcgaaata 
      gctccagct cattatctg actgattac agtgccagc 
      ggagcaccc aagcacaat aactcaccc ttcccttac 
      gactccgaa ctcaatcac aaaattatc ttatggtct 
      ggtgatatt tccgcactc caagtagat gctatagtc 
      aattccacg aacgaatcg atgaacgaa agtaatccg 
      gtcagtgat agaattttc caaagggcc ggatctgaa 
      cttaaagag gaaatcaat ttagatata agagagtgt 
      aggacggga gaagtcaga gtcacacaa ggacatgca 
      ctgccagct cgatatatt atacacacc gttggaccc 
      aaatataat ataaaatac caatctgct tcggaaaac 
      acattacat atatgttat agaaacgtt ctccagaaa 
      tcaaaagaa atgggtctc cactccatc gccctatgc 
      gtcataaac tccgtgaaa agaaactat cctcccgac 
      gaaggagca cacatagca ttacgtacg gtgaggaga 
      ttcctcgag caccacggt agttctttt gataccgtc 
      atcttcacg gtcgacaat acagattta ggtatttac 
      gaagtgcta ttacctctc tattttcca cgatctaaa 
      ttcgaggag gacgccgct cgttggcag ctaccttca 
      gacgtcgga ggacacgac ggagagccc atggtgccg 
      gacagacaa attcggatt atcgacaat ccacaacac 
      actctgcat gccgacgaa gaatctata gatttgtcg 
      tctcaactg gaaacatct ataaatgtg ggtgaacat 
      gcattcagt caaatgcag ggtgatctc gaccaacag 
      cggcttctg ggggaaaga ccctataat gatccactc 
      gccgatatt atggtcaag gaaatacaa caccaggaa 
      cgcgggatg gccgtcgaa agtccctat ccaaatgtg 
      ctcacaagc gtcaccaag tttctagga tggacaagc 
      atcgaaatc gacgaagac agatacgag cggctcttg 
      agacgtgcc aaaaccgaa gacctgacg gaggtctcc 
      ggcattggc tgtctgtac cagagcgga gtggaccga 
      ctaggtcgg ccagtggtg gtattcatc ggaaaatgg 
      tttcctttc aacaaaatt aatttagat aaggcttta 
      ttgtatcta ataacgttg ctggatccg atcgtgaag 
      ggcgactac gttatcgcc tacttccat acgcttacc 
      tccagcagc aactatccg tcgttctca tggctgaga 
      gaggtctat aatgtgctg ccttacaag tataagaag 
      aatcttaag gctttctac atcgtgcat ccgacgttt 
      tggacgaag atgatgact tggtggttt accacgttt 
      atggcaccg gccattaag caaaaagtg cacagttta 
      cccggagtg gagtacctc tatgcggtg atgtcgccc 
      gatcagttg gaaattccc gcatttatt accgaatat 
      gatatgact ataaacggc ctgaggtat tttcagccc 
      ggagcgccg acataa
    mRNA sequence
    • >TC032080 RA
      tggaatcaa accaaatct ttaattttt gtaacttgc 
      gttgcgcaa acaagtaag cactttgtg gtgggggtt 
      tccgtaaaa acgtcattg gtttgacag ttcgtgtag 
      gtgttgttt cgtgtgcta agtgtaatg gttttgtat 
      gaaaatagt aaaattctg tgaaaacgt tgatataaa 
      cgcttatac ttgatgcgg atcataatg aatcaggag 
      cccttaggg gtagcatcg gacgtggtg cacccgacc 
      gatctgccg aggtggagc gaaatagct ccagctcat 
      tatctgact gattacagt gccagcgga gcacccaag 
      cacaataac tcacccttc ccttacgac tccgaactc 
      aatcacaaa attatctta tggtctggt gatatttcc 
      gcactccaa gtagatgct atagtcaat tccacgaac 
      gaatcgatg aacgaaagt aatccggtc agtgataga 
      attttccaa agggccgga tctgaactt aaagaggaa 
      atcaattta gatataaga gagtgtagg acgggagaa 
      gtcagagtc acacaagga catgcactg ccagctcga 
      tatattata cacaccgtt ggacccaaa tataatata 
      aaataccaa tctgcttcg gaaaacaca ttacatata 
      tgttataga aacgttctc cagaaatca aaagaaatg 
      ggtctccac tccatcgcc ctatgcgtc ataaactcc 
      gtgaaaaga aactatcct cccgacgaa ggagcacac 
      atagcatta cgtacggtg aggagattc ctcgagcac 
      cacggtagt tcttttgat accgtcatc ttcacggtc 
      gacaataca gatttaggt atttacgaa gtgctatta 
      cctctctat tttccacga tctaaattc gaggaggac 
      gccgctcgt tggcagcta ccttcagac gtcggagga 
      cacgacgga gagcccatg gtgccggac agacaaatt 
      cggattatc gacaatcca caacacact ctgcatgcc 
      gacgaagaa tctatagat ttgtcgtct caactggaa 
      acatctata aatgtgggt gaacatgca ttcagtcaa 
      atgcagggt gatctcgac caacagcgg cttctgggg 
      gaaagaccc tataatgat ccactcgcc gatattatg 
      gtcaaggaa atacaacac caggaacga tacgagcgg 
      ctcttgaga cgtgccaaa accgaagac ctgacggag 
      gtctccggc attggctgt ctgtaccag agcggagtg 
      gaccgacta ggtcggcca gtggtggta ttcatcgga 
      aaatggttt cctttcaac aaaattaat ttagataag 
      gctttattg tatctaata acgttgctg gatccgatc 
      gtgaagggc gactacgtt atcgcctac ttccatacg 
      cttacctcc agcagcaac tatccgtcg ttctcatgg 
      ctgagagag gtctataat gtgctgcct tacaagtat 
      aagaagaat cttaaggct ttctacatc gtgcatccg 
      acgttttgg acgaagatg atgacttgg tggtttacc 
      acgtttatg gcaccggcc attaagcaa aaagtgcac 
      agtttaccc ggagtggag tacctctat gcggtgatg 
      tcgcccgat cagttggaa attcccgca tttattacc 
      gaatatgat atgactata aacggcctg aggtatttt 
      cagcccgga gcgccgaca taatcgccc tcaacgtta 
      gtgttcagt gccgaactt agtattcat tccctaagt 
      gataagttt gcaatattt gactagtgc taagttgtg 
      ctgtgccaa ctgatatat tagaacagt cgctgtttg 
      cagccgaca tttcatttt tcaacttcg cctctaatc 
      aattattaa tcatgagaa ctaaccgaa atatatagc 
      gtacttcta actgcttaa taaatcgat catttcatg 
      gacgacagt agtttattt gacgtattt tacttagtt 
      ttaagttga cattccgct gaaattgtt ggtttatgc 
      gaaggtcct ctgccattg gaacacttc gggcagctt 
      tgtcttgca caaattcaa ttcaaaaga ctggagttc 
      aagggtgcc aaactgttg gattgtgtc agtggtaga 
      ttgcctttc catattgtt aaattttat gtcattgtt 
      aatgttaca gcatatctt gtaagacat gtttgttat 
      tttattgtt tttgcgttt gtagacaaa tgaaattca 
      tggttagaa tacgttggg tgcgtttac gtaatttca 
      taattgttg tatcagtac ataacttac taactatat 
      tttaatctt atgtaataa ggttcgagg tactcagag 
      tgctcaact ggcgtaata gtgactaat atttaatag 
      caaaaatat taaatatcg gtcagcgat gagtgaaga 
      atgtttata gcgcctaat ttgcatctt gttgatttt 
      gattgcaat gatcgactt ttccaaacg tttgtcaat 
      gttgatgca tttatacct ctataagaa ttctcttgt 
      attctatca agaatttta ctaacgttt tcgatactg 
      ttgcttgat aacatagtc actatgttc ctgacggga 
      tactgttaa tcacgttta cacaaaaat tttgaaaat 
      ttctttaat gatcaacaa atgaacatc aagagagca 
      atatgaaga tatgaatct aaatttatt atagtgttg 
      tagttgcat attgtaatg atgttatta cttataaat 
      attttacat ggaaaaatc cagctgcga gttgttgag 
      tattaattt tataaatga caacaatat ggaataaat 
      attgtacac ctca
    • >TC032080 RB
      tggaatcaa accaaatct ttaattttt gtaacttgc 
      gttgcgcaa acaagtaag cactttgtg gtgggggtt 
      tccgtaaaa acgtcattg gtttgacag ttcgtgtag 
      gtgttgttt cgtgtgcta agtgtaatg gttttgtat 
      gaaaatagt aaaattctg tgaaaacgt tgatataaa 
      cgcttatac ttgatgcgg atcataatg aatcaggag 
      cccttaggg gtagcatcg gacgtggtg cacccgacc 
      gatctgccg aggtggagc gaaatagct ccagctcat 
      tatctgact gattacagt gccagcgga gcacccaag 
      cacaataac tcacccttc ccttacgac tccgaactc 
      aatcacaaa attatctta tggtctggt gatatttcc 
      gcactccaa gtagatgct atagtcaat tccacgaac 
      gaatcgatg aacgaaagt aatccggtc agtgataga 
      attttccaa agggccgga tctgaactt aaagaggaa 
      atcaattta gatataaga gagtgtagg acgggagaa 
      gtcagagtc acacaagga catgcactg ccagctcga 
      tatattata cacaccgtt ggacccaaa tataatata 
      aaataccaa tctgcttcg gaaaacaca ttacatata 
      tgttataga aacgttctc cagaaatca aaagaaatg 
      ggtctccac tccatcgcc ctatgcgtc ataaactcc 
      gtgaaaaga aactatcct cccgacgaa ggagcacac 
      atagcatta cgtacggtg aggagattc ctcgagcac 
      cacggtagt tcttttgat accgtcatc ttcacggtc 
      gacaataca gatttaggt atttacgaa gtgctatta 
      cctctctat tttccacga tctaaattc gaggaggac 
      gccgctcgt tggcagcta ccttcagac gtcggagga 
      cacgacgga gagcccatg gtgccggac agacaaatt 
      cggattatc gacaatcca caacacact ctgcatgcc 
      gacgaagaa tctatagat ttgtcgtct caactggaa 
      acatctata aatgtgggt gaacatgca ttcagtcaa 
      atgcagggt gatctcgac caacagcgg cttctgggg 
      gaaagaccc tataatgat ccactcgcc gatattatg 
      gtcaaggaa atacaacac caggaacgc gggatggcc 
      gtcgaaagt ccctatcca aatgtgctc acaagcgtc 
      accaagttt ctaggatgg acaagcatc gaaatcgac 
      gaagacaga tacgagcgg ctcttgaga cgtgccaaa 
      accgaagac ctgacggag gtctccggc attggctgt 
      ctgtaccag agcggagtg gaccgacta ggtcggcca 
      gtggtggta ttcatcgga aaatggttt cctttcaac 
      aaaattaat ttagataag gctttattg tatctaata 
      acgttgctg gatccgatc gtgaagggc gactacgtt 
      atcgcctac ttccatacg cttacctcc agcagcaac 
      tatccgtcg ttctcatgg ctgagagag gtctataat 
      gtgctgcct tacaagtat aagaagaat cttaaggct 
      ttctacatc gtgcatccg acgttttgg acgaagatg 
      atgacttgg tggtttacc acgtttatg gcaccggcc 
      attaagcaa aaagtgcac agtttaccc ggagtggag 
      tacctctat gcggtgatg tcgcccgat cagttggaa 
      attcccgca tttattacc gaatatgat atgactata 
      aacggcctg aggtatttt cagcccgga gcgccgaca 
      taatcgccc tcaacgtta gtgttcagt gccgaactt 
      agtattcat tccctaagt gataagttt gcaatattt 
      gactagtgc taagttgtg ctgtgccaa ctgatatat 
      tagaacagt cgctgtttg cagccgaca tttcatttt 
      tcaacttcg cctctaatc aattattaa tcatgagaa 
      ctaaccgaa atatatagc gtacttcta actgcttaa 
      taaatcgat catttcatg gacgacagt agtttattt 
      gacgtattt tacttagtt ttaagttga cattccgct 
      gaaattgtt ggtttatgc gaaggtcct ctgccattg 
      gaacacttc gggcagctt tgtcttgca caaattcaa 
      ttcaaaaga ctggagttc aagggtgcc aaactgttg 
      gattgtgtc agtggtaga ttgcctttc catattgtt 
      aaattttat gtcattgtt aatgttaca gcatatctt 
      gtaagacat gtttgttat tttattgtt tttgcgttt 
      gtagacaaa tgaaattca tggttagaa tacgttggg 
      tgcgtttac gtaatttca taattgttg tatcagtac 
      ataacttac taactatat tttaatctt atgtaataa 
      ggttcgagg tactcagag tgctcaact ggcgtaata 
      gtgactaat atttaatag caaaaatat taaatatcg 
      gtcagcgat gagtgaaga atgtttata gcgcctaat 
      ttgcatctt gttgatttt gattgcaat gatcgactt 
      ttccaaacg tttgtcaat gttgatgca tttatacct 
      ctataagaa ttctcttgt attctatca agaatttta 
      ctaacgttt tcgatactg ttgcttgat aacatagtc 
      actatgttc ctgacggga tactgttaa tcacgttta 
      cacaaaaat tttgaaaat ttctttaat gatcaacaa 
      atgaacatc aagagagca atatgaaga tatgaatct 
      aaatttatt atagtgttg tagttgcat attgtaatg 
      atgttatta cttataaat attttacat ggaaaaatc 
      cagctgcga gttgttgag tattaattt tataaatga 
      caacaatat ggaataaat attgtacac ctca