| General information | |||
| TC032080
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 6964210..6982546 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032080 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00158 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
larval musculature differentiation incomplete - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC032080 RA
MNQEPLGVA SDVVHPTDL PRWSEIAPA HYLTDYSAS GAPKHNNSP FPYDSELNH KIILWSGDI SALQVDAIV NSTNESMNE SNPVSDRIF QRAGSELKE EINLDIREC RTGEVRVTQ GHALPARYI IHTVGPKYN IKYQSASEN TLHICYRNV LQKSKEMGL HSIALCVIN SVKRNYPPD EGAHIALRT VRRFLEHHG SSFDTVIFT VDNTDLGIY EVLLPLYFP RSKFEEDAA RWQLPSDVG GHDGEPMVP DRQIRIIDN PQHTLHADE ESIDLSSQL ETSINVGEH AFSQMQGDL DQQRLLGER PYNDPLADI MVKEIQHQE RYERLLRRA KTEDLTEVS GIGCLYQSG VDRLGRPVV VFIGKWFPF NKINLDKAL LYLITLLDP IVKGDYVIA YFHTLTSSS NYPSFSWLR EVYNVLPYK YKKNLKAFY IVHPTFWTK MMTWWFTTF MAPAIKQKV HSLPGVEYL YAVMSPDQL EIPAFITEY DMTINGLRY FQPGAPT
- >TC032080 RB
MNQEPLGVA SDVVHPTDL PRWSEIAPA HYLTDYSAS GAPKHNNSP FPYDSELNH KIILWSGDI SALQVDAIV NSTNESMNE SNPVSDRIF QRAGSELKE EINLDIREC RTGEVRVTQ GHALPARYI IHTVGPKYN IKYQSASEN TLHICYRNV LQKSKEMGL HSIALCVIN SVKRNYPPD EGAHIALRT VRRFLEHHG SSFDTVIFT VDNTDLGIY EVLLPLYFP RSKFEEDAA RWQLPSDVG GHDGEPMVP DRQIRIIDN PQHTLHADE ESIDLSSQL ETSINVGEH AFSQMQGDL DQQRLLGER PYNDPLADI MVKEIQHQE RGMAVESPY PNVLTSVTK FLGWTSIEI DEDRYERLL RRAKTEDLT EVSGIGCLY QSGVDRLGR PVVVFIGKW FPFNKINLD KALLYLITL LDPIVKGDY VIAYFHTLT SSSNYPSFS WLREVYNVL PYKYKKNLK AFYIVHPTF WTKMMTWWF TTFMAPAIK QKVHSLPGV EYLYAVMSP DQLEIPAFI TEYDMTING LRYFQPGAP T
- >TC032080 RA
atgaatcag gagccctta ggggtagca tcggacgtg gtgcacccg accgatctg ccgaggtgg agcgaaata gctccagct cattatctg actgattac agtgccagc ggagcaccc aagcacaat aactcaccc ttcccttac gactccgaa ctcaatcac aaaattatc ttatggtct ggtgatatt tccgcactc caagtagat gctatagtc aattccacg aacgaatcg atgaacgaa agtaatccg gtcagtgat agaattttc caaagggcc ggatctgaa cttaaagag gaaatcaat ttagatata agagagtgt aggacggga gaagtcaga gtcacacaa ggacatgca ctgccagct cgatatatt atacacacc gttggaccc aaatataat ataaaatac caatctgct tcggaaaac acattacat atatgttat agaaacgtt ctccagaaa tcaaaagaa atgggtctc cactccatc gccctatgc gtcataaac tccgtgaaa agaaactat cctcccgac gaaggagca cacatagca ttacgtacg gtgaggaga ttcctcgag caccacggt agttctttt gataccgtc atcttcacg gtcgacaat acagattta ggtatttac gaagtgcta ttacctctc tattttcca cgatctaaa ttcgaggag gacgccgct cgttggcag ctaccttca gacgtcgga ggacacgac ggagagccc atggtgccg gacagacaa attcggatt atcgacaat ccacaacac actctgcat gccgacgaa gaatctata gatttgtcg tctcaactg gaaacatct ataaatgtg ggtgaacat gcattcagt caaatgcag ggtgatctc gaccaacag cggcttctg ggggaaaga ccctataat gatccactc gccgatatt atggtcaag gaaatacaa caccaggaa cgatacgag cggctcttg agacgtgcc aaaaccgaa gacctgacg gaggtctcc ggcattggc tgtctgtac cagagcgga gtggaccga ctaggtcgg ccagtggtg gtattcatc ggaaaatgg tttcctttc aacaaaatt aatttagat aaggcttta ttgtatcta ataacgttg ctggatccg atcgtgaag ggcgactac gttatcgcc tacttccat acgcttacc tccagcagc aactatccg tcgttctca tggctgaga gaggtctat aatgtgctg ccttacaag tataagaag aatcttaag gctttctac atcgtgcat ccgacgttt tggacgaag atgatgact tggtggttt accacgttt atggcaccg gccattaag caaaaagtg cacagttta cccggagtg gagtacctc tatgcggtg atgtcgccc gatcagttg gaaattccc gcatttatt accgaatat gatatgact ataaacggc ctgaggtat tttcagccc ggagcgccg acataa
- >TC032080 RB
atgaatcag gagccctta ggggtagca tcggacgtg gtgcacccg accgatctg ccgaggtgg agcgaaata gctccagct cattatctg actgattac agtgccagc ggagcaccc aagcacaat aactcaccc ttcccttac gactccgaa ctcaatcac aaaattatc ttatggtct ggtgatatt tccgcactc caagtagat gctatagtc aattccacg aacgaatcg atgaacgaa agtaatccg gtcagtgat agaattttc caaagggcc ggatctgaa cttaaagag gaaatcaat ttagatata agagagtgt aggacggga gaagtcaga gtcacacaa ggacatgca ctgccagct cgatatatt atacacacc gttggaccc aaatataat ataaaatac caatctgct tcggaaaac acattacat atatgttat agaaacgtt ctccagaaa tcaaaagaa atgggtctc cactccatc gccctatgc gtcataaac tccgtgaaa agaaactat cctcccgac gaaggagca cacatagca ttacgtacg gtgaggaga ttcctcgag caccacggt agttctttt gataccgtc atcttcacg gtcgacaat acagattta ggtatttac gaagtgcta ttacctctc tattttcca cgatctaaa ttcgaggag gacgccgct cgttggcag ctaccttca gacgtcgga ggacacgac ggagagccc atggtgccg gacagacaa attcggatt atcgacaat ccacaacac actctgcat gccgacgaa gaatctata gatttgtcg tctcaactg gaaacatct ataaatgtg ggtgaacat gcattcagt caaatgcag ggtgatctc gaccaacag cggcttctg ggggaaaga ccctataat gatccactc gccgatatt atggtcaag gaaatacaa caccaggaa cgcgggatg gccgtcgaa agtccctat ccaaatgtg ctcacaagc gtcaccaag tttctagga tggacaagc atcgaaatc gacgaagac agatacgag cggctcttg agacgtgcc aaaaccgaa gacctgacg gaggtctcc ggcattggc tgtctgtac cagagcgga gtggaccga ctaggtcgg ccagtggtg gtattcatc ggaaaatgg tttcctttc aacaaaatt aatttagat aaggcttta ttgtatcta ataacgttg ctggatccg atcgtgaag ggcgactac gttatcgcc tacttccat acgcttacc tccagcagc aactatccg tcgttctca tggctgaga gaggtctat aatgtgctg ccttacaag tataagaag aatcttaag gctttctac atcgtgcat ccgacgttt tggacgaag atgatgact tggtggttt accacgttt atggcaccg gccattaag caaaaagtg cacagttta cccggagtg gagtacctc tatgcggtg atgtcgccc gatcagttg gaaattccc gcatttatt accgaatat gatatgact ataaacggc ctgaggtat tttcagccc ggagcgccg acataa
- >TC032080 RA
tggaatcaa accaaatct ttaattttt gtaacttgc gttgcgcaa acaagtaag cactttgtg gtgggggtt tccgtaaaa acgtcattg gtttgacag ttcgtgtag gtgttgttt cgtgtgcta agtgtaatg gttttgtat gaaaatagt aaaattctg tgaaaacgt tgatataaa cgcttatac ttgatgcgg atcataatg aatcaggag cccttaggg gtagcatcg gacgtggtg cacccgacc gatctgccg aggtggagc gaaatagct ccagctcat tatctgact gattacagt gccagcgga gcacccaag cacaataac tcacccttc ccttacgac tccgaactc aatcacaaa attatctta tggtctggt gatatttcc gcactccaa gtagatgct atagtcaat tccacgaac gaatcgatg aacgaaagt aatccggtc agtgataga attttccaa agggccgga tctgaactt aaagaggaa atcaattta gatataaga gagtgtagg acgggagaa gtcagagtc acacaagga catgcactg ccagctcga tatattata cacaccgtt ggacccaaa tataatata aaataccaa tctgcttcg gaaaacaca ttacatata tgttataga aacgttctc cagaaatca aaagaaatg ggtctccac tccatcgcc ctatgcgtc ataaactcc gtgaaaaga aactatcct cccgacgaa ggagcacac atagcatta cgtacggtg aggagattc ctcgagcac cacggtagt tcttttgat accgtcatc ttcacggtc gacaataca gatttaggt atttacgaa gtgctatta cctctctat tttccacga tctaaattc gaggaggac gccgctcgt tggcagcta ccttcagac gtcggagga cacgacgga gagcccatg gtgccggac agacaaatt cggattatc gacaatcca caacacact ctgcatgcc gacgaagaa tctatagat ttgtcgtct caactggaa acatctata aatgtgggt gaacatgca ttcagtcaa atgcagggt gatctcgac caacagcgg cttctgggg gaaagaccc tataatgat ccactcgcc gatattatg gtcaaggaa atacaacac caggaacga tacgagcgg ctcttgaga cgtgccaaa accgaagac ctgacggag gtctccggc attggctgt ctgtaccag agcggagtg gaccgacta ggtcggcca gtggtggta ttcatcgga aaatggttt cctttcaac aaaattaat ttagataag gctttattg tatctaata acgttgctg gatccgatc gtgaagggc gactacgtt atcgcctac ttccatacg cttacctcc agcagcaac tatccgtcg ttctcatgg ctgagagag gtctataat gtgctgcct tacaagtat aagaagaat cttaaggct ttctacatc gtgcatccg acgttttgg acgaagatg atgacttgg tggtttacc acgtttatg gcaccggcc attaagcaa aaagtgcac agtttaccc ggagtggag tacctctat gcggtgatg tcgcccgat cagttggaa attcccgca tttattacc gaatatgat atgactata aacggcctg aggtatttt cagcccgga gcgccgaca taatcgccc tcaacgtta gtgttcagt gccgaactt agtattcat tccctaagt gataagttt gcaatattt gactagtgc taagttgtg ctgtgccaa ctgatatat tagaacagt cgctgtttg cagccgaca tttcatttt tcaacttcg cctctaatc aattattaa tcatgagaa ctaaccgaa atatatagc gtacttcta actgcttaa taaatcgat catttcatg gacgacagt agtttattt gacgtattt tacttagtt ttaagttga cattccgct gaaattgtt ggtttatgc gaaggtcct ctgccattg gaacacttc gggcagctt tgtcttgca caaattcaa ttcaaaaga ctggagttc aagggtgcc aaactgttg gattgtgtc agtggtaga ttgcctttc catattgtt aaattttat gtcattgtt aatgttaca gcatatctt gtaagacat gtttgttat tttattgtt tttgcgttt gtagacaaa tgaaattca tggttagaa tacgttggg tgcgtttac gtaatttca taattgttg tatcagtac ataacttac taactatat tttaatctt atgtaataa ggttcgagg tactcagag tgctcaact ggcgtaata gtgactaat atttaatag caaaaatat taaatatcg gtcagcgat gagtgaaga atgtttata gcgcctaat ttgcatctt gttgatttt gattgcaat gatcgactt ttccaaacg tttgtcaat gttgatgca tttatacct ctataagaa ttctcttgt attctatca agaatttta ctaacgttt tcgatactg ttgcttgat aacatagtc actatgttc ctgacggga tactgttaa tcacgttta cacaaaaat tttgaaaat ttctttaat gatcaacaa atgaacatc aagagagca atatgaaga tatgaatct aaatttatt atagtgttg tagttgcat attgtaatg atgttatta cttataaat attttacat ggaaaaatc cagctgcga gttgttgag tattaattt tataaatga caacaatat ggaataaat attgtacac ctca
- >TC032080 RB
tggaatcaa accaaatct ttaattttt gtaacttgc gttgcgcaa acaagtaag cactttgtg gtgggggtt tccgtaaaa acgtcattg gtttgacag ttcgtgtag gtgttgttt cgtgtgcta agtgtaatg gttttgtat gaaaatagt aaaattctg tgaaaacgt tgatataaa cgcttatac ttgatgcgg atcataatg aatcaggag cccttaggg gtagcatcg gacgtggtg cacccgacc gatctgccg aggtggagc gaaatagct ccagctcat tatctgact gattacagt gccagcgga gcacccaag cacaataac tcacccttc ccttacgac tccgaactc aatcacaaa attatctta tggtctggt gatatttcc gcactccaa gtagatgct atagtcaat tccacgaac gaatcgatg aacgaaagt aatccggtc agtgataga attttccaa agggccgga tctgaactt aaagaggaa atcaattta gatataaga gagtgtagg acgggagaa gtcagagtc acacaagga catgcactg ccagctcga tatattata cacaccgtt ggacccaaa tataatata aaataccaa tctgcttcg gaaaacaca ttacatata tgttataga aacgttctc cagaaatca aaagaaatg ggtctccac tccatcgcc ctatgcgtc ataaactcc gtgaaaaga aactatcct cccgacgaa ggagcacac atagcatta cgtacggtg aggagattc ctcgagcac cacggtagt tcttttgat accgtcatc ttcacggtc gacaataca gatttaggt atttacgaa gtgctatta cctctctat tttccacga tctaaattc gaggaggac gccgctcgt tggcagcta ccttcagac gtcggagga cacgacgga gagcccatg gtgccggac agacaaatt cggattatc gacaatcca caacacact ctgcatgcc gacgaagaa tctatagat ttgtcgtct caactggaa acatctata aatgtgggt gaacatgca ttcagtcaa atgcagggt gatctcgac caacagcgg cttctgggg gaaagaccc tataatgat ccactcgcc gatattatg gtcaaggaa atacaacac caggaacgc gggatggcc gtcgaaagt ccctatcca aatgtgctc acaagcgtc accaagttt ctaggatgg acaagcatc gaaatcgac gaagacaga tacgagcgg ctcttgaga cgtgccaaa accgaagac ctgacggag gtctccggc attggctgt ctgtaccag agcggagtg gaccgacta ggtcggcca gtggtggta ttcatcgga aaatggttt cctttcaac aaaattaat ttagataag gctttattg tatctaata acgttgctg gatccgatc gtgaagggc gactacgtt atcgcctac ttccatacg cttacctcc agcagcaac tatccgtcg ttctcatgg ctgagagag gtctataat gtgctgcct tacaagtat aagaagaat cttaaggct ttctacatc gtgcatccg acgttttgg acgaagatg atgacttgg tggtttacc acgtttatg gcaccggcc attaagcaa aaagtgcac agtttaccc ggagtggag tacctctat gcggtgatg tcgcccgat cagttggaa attcccgca tttattacc gaatatgat atgactata aacggcctg aggtatttt cagcccgga gcgccgaca taatcgccc tcaacgtta gtgttcagt gccgaactt agtattcat tccctaagt gataagttt gcaatattt gactagtgc taagttgtg ctgtgccaa ctgatatat tagaacagt cgctgtttg cagccgaca tttcatttt tcaacttcg cctctaatc aattattaa tcatgagaa ctaaccgaa atatatagc gtacttcta actgcttaa taaatcgat catttcatg gacgacagt agtttattt gacgtattt tacttagtt ttaagttga cattccgct gaaattgtt ggtttatgc gaaggtcct ctgccattg gaacacttc gggcagctt tgtcttgca caaattcaa ttcaaaaga ctggagttc aagggtgcc aaactgttg gattgtgtc agtggtaga ttgcctttc catattgtt aaattttat gtcattgtt aatgttaca gcatatctt gtaagacat gtttgttat tttattgtt tttgcgttt gtagacaaa tgaaattca tggttagaa tacgttggg tgcgtttac gtaatttca taattgttg tatcagtac ataacttac taactatat tttaatctt atgtaataa ggttcgagg tactcagag tgctcaact ggcgtaata gtgactaat atttaatag caaaaatat taaatatcg gtcagcgat gagtgaaga atgtttata gcgcctaat ttgcatctt gttgatttt gattgcaat gatcgactt ttccaaacg tttgtcaat gttgatgca tttatacct ctataagaa ttctcttgt attctatca agaatttta ctaacgttt tcgatactg ttgcttgat aacatagtc actatgttc ctgacggga tactgttaa tcacgttta cacaaaaat tttgaaaat ttctttaat gatcaacaa atgaacatc aagagagca atatgaaga tatgaatct aaatttatt atagtgttg tagttgcat attgtaatg atgttatta cttataaat attttacat ggaaaaatc cagctgcga gttgttgag tattaattt tataaatga caacaatat ggaataaat attgtacac ctca
