Status
Please wait ...
Gene information
General information
TC000693 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 1932716..1934925
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000693
    iBeetle screen iB_00156
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature differentiation incomplete - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 42.86% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Pupal morphology
    abdomen gin-traps partially orientation irregular - Number of animals showing the phenotype: 6

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000693 RA
      MVVLEGGGM LSTSGNQYL QPDYLSPLP TTLDAKKSP 
      LALLAQTCS QIGADTPSS KPLISPLEK PKKSQENST 
      RSPPVKNDK SAQRNTPTE NKLAFKPYE NNVVTKKSE 
      DNRPNSKSG STHQNSTPS DEKKSETQS PERKSVSPA 
      TSTAATPAP ETGKTSPGS SENKSSTPS ARGASPIIR 
      SGLEILQGH PKDTPLGAY KHGALSSGL SSGLNGPLG 
      SLCCPPGLE HTNPAFRPP FPGAPFSHH HAAMLAAGY 
      PGATPGPYL SYARVKTPS GGEALVPVC KDPYCTSCQ 
      FSMHNQQLL MSGASGPCP SGCTQCDHQ KYNLAMAMS 
      LVPPGPVPS SLAYPQLGR PYVCNWIAG DTYCGKRFV 
      TSEELLQHL RTHTSGSDG SAAVTSSAS PLLNPHSLF 
      SSAASLHRP GYPNPPLSP LSAARYHPY GKPTLPTSL 
      GASPYSAFN PTALGAYYS PYSVYGQRL GAAAVHP
    CDS sequence
    • >TC000693 RA
      atggttgtg ttggagggt ggaggcatg ctgtcaacg 
      tccggaaat caatatttg cagccggat tacctatca 
      ccgttaccc actacgttg gatgctaag aaaagtccg 
      ttggcactc ctcgcccaa acatgcagt caaatagga 
      gcagacact ccatcgtcc aagcctttg atttctcca 
      ttggagaaa ccaaagaag tcgcaagag aattcgacg 
      cgttcgcct ccggtaaaa aacgataag agtgctcaa 
      agaaacaca ccgactgaa aataagctc gcttttaaa 
      ccatatgaa aataatgtt gttacgaaa aaaagcgaa 
      gacaaccgg cctaactct aaatcagga tccactcat 
      caaaattcg acaccaagt gacgagaag aaatcagaa 
      acgcaaagt cctgaacgg aagtcagtg tcaccagca 
      accagcacc gctgcgaca ccggccccc gaaaccggg 
      aagacttcc ccaggttcg agtgaaaac aaatcgtca 
      actccgtcg gctcgtggt gcaagcccc atcatcaga 
      tccggtctg gaaattctg caaggacat ccgaaagat 
      acacctttg ggggcgtac aaacacggt gctttatcg 
      tccgggtta tcgtcagga ctgaacggc cctttggga 
      tcgctgtgt tgtcctccc ggactcgaa cacacgaat 
      ccagctttc aggcccccg tttcccgga gctccgttt 
      tcgcaccac catgccgcc atgctcgct gccggctat 
      ccgggagcc actccggga ccgtacttg agttacgcg 
      cgtgtcaaa actccatca ggtggtgaa gctttagtt 
      ccagtgtgt aaagaccct tattgcacc agttgccaa 
      ttcagcatg cacaatcag cagttgttg atgagtgga 
      gcatctggc ccttgtccc agtggctgc acgcaatgc 
      gaccaccag aagtataat ttggccatg gcgatgtcg 
      ttagtgccc ccgggtccc gtgccgtcg tcacttgct 
      taccctcag ctgggacgg ccatatgtg tgtaattgg 
      atagcgggg gacacctac tgcgggaag aggttcgtc 
      acatctgaa gaactgttg caacatttg agaacacac 
      acgagtgga tctgacggg tcggcggcg gtgacgtcc 
      tcggcatcg cctttactt aacccccat tcgttgttc 
      tcgtcggcg gcgtcgctc cataggcct gggtatccc 
      aatcctccg cttagcccg ttatcggca gcgaggtat 
      cacccgtat gggaaaccc actctacct acctctttg 
      ggagcttcg ccatacagt gcttttaat ccgacggct 
      cttggagct tattactct ccgtattcc gtttacgga 
      caacggctt ggcgctgct gctgtgcat ccatag
    mRNA sequence
    • >TC000693 RA
      ataaaataa ataaagctc aaccgtatt ttacgaccg 
      acaggttgt aataacttc gcagtgaac tgcgccacc 
      atccatccg tcatcattg tgcttttgg gcggctaac 
      atcaaagca tccagaagg tctgtcgtc gtcacttca 
      tgggtggta gaaaccgac aggccagaa acgtggctg 
      tattagcgc tttgtcatg ctaggactt tcagcaggg 
      ctgcaaagc ctggagggg ctgccaaac ctgcgcaat 
      agcctctcg tttagagag cggagctct gttgccttc 
      agactttca tactggagt tgtccgtcg tgtgaaagc 
      tagtttgcg ctcgtgttc cgtcagttt attaatcgt 
      gtaattgtt tttatccgc cgtaaacgt gcatttatt 
      ccaaatggt tgtgttgga gggtggagg catgctgtc 
      aacgtccgg aaatcaata tttgcagcc ggattacct 
      atcaccgtt acccactac gttggatgc taagaaaag 
      tccgttggc actcctcgc ccaaacatg cagtcaaat 
      aggagcaga cactccatc gtccaagcc tttgatttc 
      tccattgga gaaaccaaa gaagtcgca agagaattc 
      gacgcgttc gcctccggt aaaaaacga taagagtgc 
      tcaaagaaa cacaccgac tgaaaataa gctcgcttt 
      taaaccata tgaaaataa tgttgttac gaaaaaaag 
      cgaagacaa ccggcctaa ctctaaatc aggatccac 
      tcatcaaaa ttcgacacc aagtgacga gaagaaatc 
      agaaacgca aagtcctga acggaagtc agtgtcacc 
      agcaaccag caccgctgc gacaccggc ccccgaaac 
      cgggaagac ttccccagg ttcgagtga aaacaaatc 
      gtcaactcc gtcggctcg tggtgcaag ccccatcat 
      cagatccgg tctggaaat tctgcaagg acatccgaa 
      agatacacc tttgggggc gtacaaaca cggtgcttt 
      atcgtccgg gttatcgtc aggactgaa cggcccttt 
      gggatcgct gtgttgtcc tcccggact cgaacacac 
      gaatccagc tttcaggcc cccgtttcc cggagctcc 
      gttttcgca ccaccatgc cgccatgct cgctgccgg 
      ctatccggg agccactcc gggaccgta cttgagtta 
      cgcgcgtgt caaaactcc atcaggtgg tgaagcttt 
      agttccagt gtgtaaaga cccttattg caccagttg 
      ccaattcag catgcacaa tcagcagtt gttgatgag 
      tggagcatc tggcccttg tcccagtgg ctgcacgca 
      atgcgacca ccagaagta taatttggc catggcgat 
      gtcgttagt gcccccggg tcccgtgcc gtcgtcact 
      tgcttaccc tcagctggg acggccata tgtgtgtaa 
      ttggatagc gggggacac ctactgcgg gaagaggtt 
      cgtcacatc tgaagaact gttgcaaca tttgagaac 
      acacacgag tggatctga cgggtcggc ggcggtgac 
      gtcctcggc atcgccttt acttaaccc ccattcgtt 
      gttctcgtc ggcggcgtc gctccatag gcctgggta 
      tcccaatcc tccgcttag cccgttatc ggcagcgag 
      gtatcaccc gtatgggaa acccactct acctacctc 
      tttgggagc ttcgccata cagtgcttt taatccgac 
      ggctcttgg agcttatta ctctccgta ttccgttta 
      cggacaacg gcttggcgc tgctgctgt gcatccata 
      gtgatactt gtagtaatt tctctcatg ttttgactc 
      atacagaat ggcgtctgt atcggtctg tttgctcca 
      ctgtgataa cgtgtaaat aatttgtcg aagctaaag 
      ctgattaaa attaagtgc aatctgaag cggacttaa 
      tcatagggc aataagtta taaaacagt ccattttct 
      ttctaagtt gttctcccg tttcaacga tactttaat 
      ctttaaata attcaataa aaatatttg tatagactt 
      gtaagtatt taatgttga gtttcgata ttgctttaa 
      taaaatgtt tgtagaacg