Status
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Gene information
General information
TC000689 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7565387..7578169
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000689
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00155

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva mostly everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 11.11% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000689 RA
      MLHLVLLLP FLISPLAAL LEGPNVCTT QESYTTTVR 
      VSEQQPYQV REYVWCLNV PPRCSKYKI KFKTVYKTQ 
      NLVKFRPVD ECCKGYTKS SSEDRCIPV CSHDCVHGT 
      CTAPEQCLC ESGYGGPYC DITCPKGLW GRECQNHCE 
      CQNNSTCDP FDGTCRCAR GWIGTHCEM KCHKGSYGQ 
      DCAEKCRCV HGECDHVSG ECRCSSGWT GPLCDDSCP 
      LGKHGSECK SECRCQNDG TCDPETGQC FCKPGWTGP 
      VCANRCPFG FWGENCAQP CGCYNGASC HHVTGECEC 
      QPGFTGDKC LDTCPEGTW GLNCSEACA CQNGAKCSN 
      LNGQCACSH GWEGKFCEA RACPDDFWG YKCKNPCQC 
      NRDNTEMCH PWTGECICK AGWNSKDCS RPCPLLTYG 
      KGCHGLCKC KNNAQCSPV NGTCLCPPG FTGDDCGQT 
      CPDNTFGED CSQKCDCKN GATCSSETG QCQCTAGWE 
      GQQCDRPCS NYSYGIQCT KQCACKNGA SCNPQNGTC 
      TCGAGFTGQ FCENKCESG FFGVNCEQR CQCEQGNYI 
      GCDPVSGKC ICKPDWKGV RCESRCPPG KFGPDCKEI 
      CNCKNNSSC DPESGKCYC ARGWQGEDC SQPCDKGYY 
      GIGCKEVCP EVSEIAMGN KTCDHITGE YVCPAGYIG 
      ITCEHPCPL GTFGKNCKN NCSCRNGGD CHHVTGECQ 
      CLPGWVGKN CTTPCEPGR FGMNCSQHC KCSNKGQCR 
      RNDGVCKCQ PGWTGTQCT EICPEGYYG DHCMAPCEC 
      KNDNFVCHP AKGCVCRHG FDGDDCDTP ILLSRVHST 
      EENSNIGSV IAGVIVAII AIVAAGCIF VYYRRRVSN 
      LKTEIAHVQ YIADPSAFS PDRNHFDNP VYSYQGGAK 
      RDNEHLLNN SKTIRNNLH KPTNTNLER ARLGVACCS 
      TDDDDLKGS YCSDELQSL KNREADATN PNIYHSIDK 
      IDHVYDEIK QKDVKDIEL EYDHLDYTR PVTTLKPHY 
      QRMASPFGS RDLTKGDKS ETE
    CDS sequence
    • >TC000689 RA
      atgttacat ttggtgtta ttacttcca tttttaatt 
      agcccttta gcagccctt ttagaaggt cccaatgta 
      tgcacgacg caagagtcg tatacgacg actgttcga 
      gtttcggaa caacaacca tatcaagtg agagaatac 
      gtgtggtgt ctgaatgtt ccacccaga tgttcaaag 
      tacaaaatc aagttcaaa accgtgtac aaaacacaa 
      aatttagtt aaatttaga ccggtggat gaatgctgc 
      aaaggatac acgaaaagc agctcggag gataggtgt 
      ataccggtg tgttcgcat gactgtgtt catggaaca 
      tgtacagca cccgaacag tgtctttgt gagagcggc 
      tacggggga ccttactgt gatattaca tgccccaaa 
      ggcttatgg ggtcgcgag tgccaaaac cactgcgaa 
      tgccagaat aactccacg tgcgacccg tttgatgga 
      acatgtcgt tgcgctcgt ggttggatc ggcacacat 
      tgcgaaatg aaatgtcat aaaggtagc tacggtcaa 
      gactgtgcg gagaaatgt aggtgtgtt cacggcgaa 
      tgtgatcac gtctcgggg gaatgtcgt tgcagctct 
      ggctggaca ggaccactt tgcgacgac tcttgtcct 
      ctcggcaaa cacggaagc gaatgcaag tcggaatgt 
      cggtgccag aacgacgga acttgcgat cccgaaacg 
      ggtcaatgt ttctgcaaa ccgggctgg actggcccc 
      gtttgcgca aacaggtgt cctttcggg ttctggggc 
      gaaaattgc gcccagcct tgcgggtgc tacaacggc 
      gcttcctgc catcatgtc actggggag tgcgagtgt 
      caacctggc tttacaggg gacaagtgc ttggatact 
      tgtccggag ggaacgtgg ggcttgaac tgtagtgag 
      gcttgtgcc tgtcagaat ggggctaaa tgctcgaat 
      ttaaacgga cagtgtgcg tgttcgcac gggtgggaa 
      ggcaagttt tgcgaggct agggcttgt cctgacgat 
      ttttggggc tacaagtgc aaaaatccc tgccagtgc 
      aatcgcgac aacacggaa atgtgtcat ccgtggacg 
      ggggaatgt atttgcaag gctgggtgg aatagtaag 
      gattgttcc agaccgtgt cctttactt acttacggt 
      aaagggtgt cacggtttg tgcaaatgt aagaacaac 
      gctcaatgt tcgcctgtt aatggcacg tgtttgtgt 
      cctcccggg ttcactggg gatgattgt ggccaaact 
      tgtcccgat aataccttc ggagaggat tgttcacag 
      aaatgcgat tgtaaaaat ggagcaacc tgttcgagc 
      gagacagga cagtgtcag tgtaccgct ggttgggaa 
      ggtcagcag tgtgatcga ccctgcagt aactattcc 
      tacggtata caatgtact aaacagtgt gcttgcaaa 
      aatggagct tcatgtaat ccccaaaat ggaacttgt 
      acctgtgga gcaggattt actggtcag ttttgcgaa 
      aataaatgc gagtcggga ttttttggc gttaactgc 
      gaacaaagg tgccaatgc gaacaagga aattacatt 
      ggctgcgat ccagtcagt ggcaaatgc atttgtaaa 
      ccagactgg aaaggtgtc cgttgcgaa agtcggtgt 
      ccgccgggc aagttcggc cccgattgc aaagaaatt 
      tgcaattgc aagaacaac agctcgtgt gacccagaa 
      tctgggaaa tgttactgt gccagaggg tggcaaggg 
      gaagactgt tcccaaccg tgtgacaag ggttattat 
      ggcatcggt tgcaaggaa gtatgtccc gaagttagc 
      gaaatagcc atggggaac aagacatgt gaccacatc 
      actggagaa tacgtctgt ccagctggt tatatcggg 
      atcacatgc gagcatccc tgtccactt gggacattc 
      ggcaaaaac tgcaaaaat aactgctcg tgccgaaac 
      ggcggtgac tgccaccat gtcacaggt gagtgccag 
      tgtttgccg ggttgggtg ggcaaaaac tgcactaca 
      ccttgtgaa cctggaaga ttcggaatg aactgtagc 
      caacattgc aagtgttcg aataagggt cagtgtcgc 
      cgaaacgat ggtgtttgc aaatgtcag cctggatgg 
      acgggaacc cagtgcact gaaatttgc cccgaggga 
      tattacggg gatcattgc atggctcca tgcgaatgc 
      aagaacgat aatttcgtc tgtcaccca gcgaagggt 
      tgcgtttgc cgtcacggt ttcgacggc gacgattgt 
      gacacaccc attcttttg agtcgagtt cactcgact 
      gaagaaaat tcaaacatt ggtagtgtt atcgctggt 
      gttattgtt gccattatt gcaatagtg gccgccggc 
      tgtattttt gtatactac cggagacgc gtatcaaat 
      ctcaagact gaaattgct catgtacaa tatatagct 
      gatcctagc gcgttttcg ccggatcgc aatcatttc 
      gacaatcct gtctattcg tatcaaggt ggtgccaaa 
      cgagacaac gaacatctc ttgaacaat tccaaaaca 
      atacgtaac aatttgcac aaaccgacg aatacgaat 
      ttggaacga gccagatta ggggtggct tgttgctcg 
      actgacgat gatgacttg aagggtagt tactgttcg 
      gatgaatta cagtcgctg aaaaacaga gaggctgat 
      gctacgaat ccgaatatt tatcacagt attgacaaa 
      atcgatcat gtttatgac gaaatcaaa cagaaggat 
      gttaaagat atagagttg gagtatgat cacttagat 
      tatacaagg ccggtaact acgttaaaa cctcattat 
      cagaggatg gcgagtccg tttgggtcg agagacttg 
      acgaaaggt gataaatcg gagactgaa tga
    mRNA sequence
    • >TC000689 RA
      ttttcaatt gtgttggag gcggcttta tcaaatatt 
      gtgataaat gattagttc gatttttat tcaattgaa 
      ttttaattt gactcacat gttgtcctt gtctaattt 
      cgactgaac cgacaagcg cagaattga ctatgcaac 
      tgacgcatg tgcagttgt taggttaat tttttgaca 
      tgttgcttt cttcagttt gtgttggtt gatatgctg 
      gttcactta cgtttagtt attttttta gtgcaacat 
      ttgtgtttt taatttaat ttagttaac tcataagta 
      gccgtttac caagttttt ttactgaag tgccacaaa 
      ttctgtgtt ttcttaatt ttatacgac tggatggat 
      ttgtcttcc ttatcatgc acaaacagg atgggcgca 
      gttttataa cttttcaac aacgctaaa tgactttcg 
      tcaaactca aacaaaatg ttacatttg gtgttatta 
      cttccattt ttaattagc cctttagca gccctttta 
      gaaggtccc aatgtatgc acgacgcaa gagtcgtat 
      acgacgact gttcgagtt tcggaacaa caaccatat 
      caagtgaga gaatacgtg tggtgtctg aatgttcca 
      cccagatgt tcaaagtac aaaatcaag ttcaaaacc 
      gtgtacaaa acacaaaat ttagttaaa tttagaccg 
      gtggatgaa tgctgcaaa ggatacacg aaaagcagc 
      tcggaggat aggtgtata ccggtgtgt tcgcatgac 
      tgtgttcat ggaacatgt acagcaccc gaacagtgt 
      ctttgtgag agcggctac gggggacct tactgtgat 
      attacatgc cccaaaggc ttatggggt cgcgagtgc 
      caaaaccac tgcgaatgc cagaataac tccacgtgc 
      gacccgttt gatggaaca tgtcgttgc gctcgtggt 
      tggatcggc acacattgc gaaatgaaa tgtcataaa 
      ggtagctac ggtcaagac tgtgcggag aaatgtagg 
      tgtgttcac ggcgaatgt gatcacgtc tcgggggaa 
      tgtcgttgc agctctggc tggacagga ccactttgc 
      gacgactct tgtcctctc ggcaaacac ggaagcgaa 
      tgcaagtcg gaatgtcgg tgccagaac gacggaact 
      tgcgatccc gaaacgggt caatgtttc tgcaaaccg 
      ggctggact ggccccgtt tgcgcaaac aggtgtcct 
      ttcgggttc tggggcgaa aattgcgcc cagccttgc 
      gggtgctac aacggcgct tcctgccat catgtcact 
      ggggagtgc gagtgtcaa cctggcttt acaggggac 
      aagtgcttg gatacttgt ccggaggga acgtggggc 
      ttgaactgt agtgaggct tgtgcctgt cagaatggg 
      gctaaatgc tcgaattta aacggacag tgtgcgtgt 
      tcgcacggg tgggaaggc aagttttgc gaggctagg 
      gcttgtcct gacgatttt tggggctac aagtgcaaa 
      aatccctgc cagtgcaat cgcgacaac acggaaatg 
      tgtcatccg tggacgggg gaatgtatt tgcaaggct 
      gggtggaat agtaaggat tgttccaga ccgtgtcct 
      ttacttact tacggtaaa gggtgtcac ggtttgtgc 
      aaatgtaag aacaacgct caatgttcg cctgttaat 
      ggcacgtgt ttgtgtcct cccgggttc actggggat 
      gattgtggc caaacttgt cccgataat accttcgga 
      gaggattgt tcacagaaa tgcgattgt aaaaatgga 
      gcaacctgt tcgagcgag acaggacag tgtcagtgt 
      accgctggt tgggaaggt cagcagtgt gatcgaccc 
      tgcagtaac tattcctac ggtatacaa tgtactaaa 
      cagtgtgct tgcaaaaat ggagcttca tgtaatccc 
      caaaatgga acttgtacc tgtggagca ggatttact 
      ggtcagttt tgcgaaaat aaatgcgag tcgggattt 
      tttggcgtt aactgcgaa caaaggtgc caatgcgaa 
      caaggaaat tacattggc tgcgatcca gtcagtggc 
      aaatgcatt tgtaaacca gactggaaa ggtgtccgt 
      tgcgaaagt cggtgtccg ccgggcaag ttcggcccc 
      gattgcaaa gaaatttgc aattgcaag aacaacagc 
      tcgtgtgac ccagaatct gggaaatgt tactgtgcc 
      agagggtgg caaggggaa gactgttcc caaccgtgt 
      gacaagggt tattatggc atcggttgc aaggaagta 
      tgtcccgaa gttagcgaa atagccatg gggaacaag 
      acatgtgac cacatcact ggagaatac gtctgtcca 
      gctggttat atcgggatc acatgcgag catccctgt 
      ccacttggg acattcggc aaaaactgc aaaaataac 
      tgctcgtgc cgaaacggc ggtgactgc caccatgtc 
      acaggtgag tgccagtgt ttgccgggt tgggtgggc 
      aaaaactgc actacacct tgtgaacct ggaagattc 
      ggaatgaac tgtagccaa cattgcaag tgttcgaat 
      aagggtcag tgtcgccga aacgatggt gtttgcaaa 
      tgtcagcct ggatggacg ggaacccag tgcactgaa 
      atttgcccc gagggatat tacggggat cattgcatg 
      gctccatgc gaatgcaag aacgataat ttcgtctgt 
      cacccagcg aagggttgc gtttgccgt cacggtttc 
      gacggcgac gattgtgac acacccatt cttttgagt 
      cgagttcac tcgactgaa gaaaattca aacattggt 
      agtgttatc gctggtgtt attgttgcc attattgca 
      atagtggcc gccggctgt atttttgta tactaccgg 
      agacgcgta tcaaatctc aagactgaa attgctcat 
      gtacaatat atagctgat cctagcgcg ttttcgccg 
      gatcgcaat catttcgac aatcctgtc tattcgtat 
      caaggtggt gccaaacga gacaacgaa catctcttg 
      aacaattcc aaaacaata cgtaacaat ttgcacaaa 
      ccgacgaat acgaatttg gaacgagcc agattaggg 
      gtggcttgt tgctcgact gacgatgat gacttgaag 
      ggtagttac tgttcggat gaattacag tcgctgaaa 
      aacagagag gctgatgct acgaatccg aatatttat 
      cacagtatt gacaaaatc gatcatgtt tatgacgaa 
      atcaaacag aaggatgtt aaagatata gagttggag 
      tatgatcac ttagattat acaaggccg gtaactacg 
      ttaaaacct cattatcag aggatggcg agtccgttt 
      gggtcgaga gacttgacg aaaggtgat aaatcggag 
      actgaatga agatattga gatgacata tttgtatat 
      taatgtgtg tgttcgttt ttaacattt aaaatgctc 
      attaacggt aatagatgt agatctccc attaagcat 
      ttactttta actcatgtc attttatat atgcaagcg 
      ataacgtac tattttatg tatagtatg tgtaatttt 
      cttaggaga tattctcta attgataag taatatttt 
      aagtgagtg tatgtttat tatgtttta tttcgtttt 
      gcaatatgt gtatcagag caataattt tcttagcat 
      atcaaatat taagtaagg tgaagatat gtataatgt 
      aggatttaa ttttccaac ttattttaa gtaatgatg 
      tggattacc cacttttgt catttaatg atatgtata 
      tatactttc cttttaatt tatgtaata ccttctgtg 
      ataagtata aaataaatt ttatctttg aac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY