General information | |||
TC000685 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 7597187..7605179 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC000685 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00152 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature differentiation incomplete - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 2
ovary without detectable defects - Number of animals showing the phenotype: 2
- >TC000685 RA
MDSVWARDP TEGFILARI SELLVEGAE VIPVNSKFP KRVLPFSDI FKAQEDDVN KDYDDNCEM VFLNEGSLL NNIRIRYYQ NKIYTYVAN ILIAVNPYK DIPQLYSPQ TIKNYQGKS LGQMPPHVF AIADKAFRD MKTLKQSQS IIVSGESGA GKTESTKHL LKYLCVNWG AQAGPIEQK ILDANPVLE AFGNAKTTR NNNSSRFGK FMEVHFDKN FHVAGGHIS HYLLEKSRI CSPQGDERS YHIFYMMMA GAPEGLRQK LALTKPDDF NYLKSGCTR YFTTAANEK KLTPGQKSA DHSKNGPLK DILIDDIAD FLTLDGALS RLGLSDSDR FDIYSTVAA VLHLGNIEF EENPEDTRG GCRVSPSKA KSLEIASGL LGIDPTELR QALEARVMQ SSRGGAKGT VIMVPLKVY EANNARDAL AKAIYSNLF DYIVNRINQ SIPFQTSSY YIGVLDIAG FEFFTVNSF EQFCINYCN EKLQQFFNE VILKNEQEL YKREGLSVP EVNFVDNQD CINLIETKS NGILQILDE ESKLPKPSY SHFTSEVHK HWPNEFRIG LPRSSKLKS HRSLRDDEG FLIRHFAGA VCYQTKQFI DKNNDALHA SLEGIVQDS KSKLIQTLF ATSNAQKGK LTFISVGNK FKTQLLELM EKLRKNGTN FIRCIKPNN KMLDNQFDG ALCLMQLKC LGMTSVLEL MEHGYPSRA PFTDLYNMY KEYLPPELK KLNPKTFCE AMLHSLKLK DRDFKFGVT RVFFRPGKF AEFDKIMKS DPENLKAII ADVKKFLVK SRWTKAQFC ALTVIKLKN KIIYRKRAL VVVQKTVKG YLTRKRHGP RIKALTKIK SLDGNLKKM EAIAGQLKK EKEATTNEI NQLKTEISG TIGRIKKED KMAASALDG LYKQLVAKV DKQMDSLQK KLLEQKNAE EQARLKKIQ EEMEREKKR KEEEERRLR EEEENRKKK AEMEARRKK EEEERKRQE EADRRTAEL LQAQQAKDE LDERRYQEQ LEQERQDHE LALRLAQES NGQLEESPP MIRKSEAVK NQQAALAKG KYDLTKWKY SELRDTINT SCDIELLEA CRHEFHRRL KVYHAWKAK NRKRTIMDE NERAPRSVM ESAARTPKI QQKAPVLDN NSQRYFRIP FVRPGGDNS KRGWWYAHF DGQYVARQI ELHPEKAPI LLVAGKDDM QMCELSLEE TGLTRKRGA EILESEFNK EWEKHGGAK YVRPADRKQ
- >TC000685 RA
atggactcg gtttgggct cgggaccct accgaaggg ttcatcctt gcccgaatt tccgaactt ttagtagag ggtgctgaa gtaatcccc gtcaactcg aaattcccc aaacgggtc ttgcctttc agcgacatt ttcaaagca caagaagat gacgtaaat aaagactac gatgacaac tgcgaaatg gttttccta aacgaaggt tcactcctc aacaatatc cgtatcaga tattaccaa aataaaatc tacacctac gtggcaaac atcctaatc gccgtcaac ccctacaaa gacatcccc caactctac tccccccag acaatcaaa aactaccaa ggcaaatct ttgggccaa atgccaccc cacgttttc gcgatcgcc gacaaggct tttcgcgat atgaaaacc ctgaagcaa tcccaatcg attatagtt tccggagag tcaggagct ggcaaaacc gaatccacc aaacatctc ctcaaatat ctctgtgtc aattggggg gcgcaagcg ggtcccatt gaacagaaa atcctcgac gcgaatcca gtccttgaa gctttcggt aacgcgaaa acaacgcgg aacaacaat agttcccgt ttcgggaaa ttcatggaa gtgcacttc gataaaaac ttccacgtg gctggagga cacatttcc cactacctt ctggaaaag tcgcggatt tgttcgccg caaggcgac gaacgcagc tatcacatt ttttacatg atgatggct ggagctccc gaggggtta agacaaaaa ctggccttg actaaaccc gacgatttt aattatctc aaatcgggt tgtacgcga tatttcacc accgccgcc aatgagaaa aagttaact ccgggccag aaaagcgca gatcattcc aaaaacggt cctttgaag gacatttta atcgacgat attgccgat tttctgacc cttgatggt gctttatca cggttgggt ttgagtgat tctgaccgg tttgacatc tactcgact gtggcggca gttttgcac cttggcaac atcgagttt gaggaaaac ccagaagat acaagaggt ggttgcaga gtgtcacca agtaaagct aaatcactc gaaattgca tctgggctt ttgggcatt gacccaact gagcttcga caagccttg gaagctcga gtcatgcaa agtagcaga ggtggggcc aaaggaaca gtcatcatg gttccgctt aaagtttac gaagcgaat aatgctcgc gacgctctc gcgaaagct atctacagc aaccttttc gattacatt gtgaatcga atcaatcag agcattccg ttccaaacc tccagctac tacatcggt gtgttagac atagccggg tttgagttt ttcactgtt aacagtttt gagcagttt tgtatcaac tactgtaac gaaaagctc caacagttc tttaacgaa gtgattttg aaaaacgag caagaactg tacaaacgt gaaggtttg agcgtgcct gaggtgaac ttcgtggat aatcaagac tgcattaat ttaattgaa actaagagt aacggaatt ctccaaatc cttgacgaa gaatccaaa ctgccgaaa ccttcctac agtcatttt acgtcagaa gtgcacaaa cactggcct aacgagttc aggattggt ttaccgaga tcgtccaaa ctgaaatcg caccgtagt cttcgggac gatgaaggt ttcctaatc cggcatttc gccggtgct gtgtgttat caaaccaaa caattcatt gataaaaac aatgacgcc ttgcacgca tcactggaa gggatagtc caagacagc aaaagcaag ctgatccaa actcttttc gccacatca aatgcacaa aaggggaaa ctcacgttt ataagcgtc ggcaataaa ttcaaaacg cagttgctc gaactgatg gaaaagttg aggaagaat gggaccaat tttatccgt tgtatcaaa ccgaataat aaaatgctc gacaatcaa ttcgacggt gccttgtgt ttgatgcaa ctgaagtgc cttggcatg acctcagtt ttggagctc atggagcat gggtacccc agtcgggcc ccatttacc gatttgtac aacatgtac aaagagtat cttccgcca gaattgaag aaactcaac cctaagacg ttttgtgaa gcaatgctc cacagtttg aaactaaaa gaccgggat ttcaaattc ggagtcacc cgagtgttt ttccggccg ggaaaattc gccgaattt gataaaatc atgaaatct gatcccgag aatttgaaa gcgatcatc gctgatgtt aagaagttc cttgtcaag tcgcggtgg accaaagca cagttctgt gccctcact gttatcaaa ttgaaaaac aaaattatt taccggaaa cgtgccctc gttgtcgta caaaagact gtgaaaggt tacttgact cgaaaacgc cacggcccg aggattaaa gctttgacc aaaattaag agtcttgat ggtaattta aagaagatg gaagcgatt gctgggcaa ttgaagaaa gagaaagaa gcaacaact aatgaaatt aaccaactt aaaactgaa ataagtggg acgattggg cgtattaag aaggaggat aaaatggca gcttccgcc ctggacggt ctttacaag cagttggtt gcgaaagtt gacaagcaa atggattct ttgcagaag aagttgcta gaacagaag aatgcggaa gagcaagcc cgattgaag aaaatccag gaagaaatg gaacgtgag aagaagcgc aaagaggag gaagaacgt cgattgagg gaagaagaa gagaacagg aagaagaag gccgaaatg gaagcccgc cgaaagaag gaagaagaa gaacgcaaa cgccaagag gaagccgat cgaagaaca gccgaactt cttcaagcg caacaagca aaagacgaa ctggatgaa cgccgctat caggaacaa ttggagcaa gaacgccaa gaccacgaa cttgctctc cgtctagca caagaaagt aacggacaa ctcgaagag agtcctccc atgattcga aaatcagaa gctgtgaag aaccaacaa gcggccctc gccaaaggc aagtacgac ctcaccaag tggaaatat tcggaactg cgcgacacc atcaacact tcctgtgat atcgaactg ctggaagct tgccgtcac gaattccac cgccgcttg aaggtgtac cacgcctgg aaggccaag aaccgcaaa cgcaccatc atggacgag aacgaacgc gccccgcgc tcagtcatg gaatcagcc gccaggaca ccaaagatc caacaaaaa gcaccagtt ttggacaat aactcccaa cggtacttc cgcattccc ttcgtacgt ccaggaggg gataacagt aaaaggggt tggtggtat gcgcatttc gatggccag tatgtagca aggcagatc gagctgcat ccggagaag gcgccgatt ttgcttgtt gcaggtaaa gacgatatg caaatgtgt gaactttcg ttggaagaa acgggattg acgaggaaa cgaggggcg gaaattttg gaaagcgag tttaataag gagtgggag aagcacggt ggagccaag tatgtgcga ccggctgat cgaaaacaa tag
- >TC000685 RA
aaagcctta aaagtgtgg taaatcgcc caccaatcg aaatctctt tgtattaca ttcgaactc aagcgcttt ccatgttgt ttcgctgag acgtgcgca ctcggcgga cattttaat tcccattta aaaattctt cgctactcg cggtgtgtg gaacggaaa gagcaaaca caaacacag tgctgggtc atatgtgcg caaggtgtt cgtcaaggt ggttttatc gcaacttta cggtagttt gttaataaa actaggagg atttagcgg aaaacagag tgaaacatg gactcggtt tgggctcgg gaccctacc gaagggttc atccttgcc cgaatttcc gaactttta gtagagggt gctgaagta atccccgtc aactcgaaa ttccccaaa cgggtcttg cctttcagc gacattttc aaagcacaa gaagatgac gtaaataaa gactacgat gacaactgc gaaatggtt ttcctaaac gaaggttca ctcctcaac aatatccgt atcagatat taccaaaat aaaatctac acctacgtg gcaaacatc ctaatcgcc gtcaacccc tacaaagac atcccccaa ctctactcc ccccagaca atcaaaaac taccaaggc aaatctttg ggccaaatg ccaccccac gttttcgcg atcgccgac aaggctttt cgcgatatg aaaaccctg aagcaatcc caatcgatt atagtttcc ggagagtca ggagctggc aaaaccgaa tccaccaaa catctcctc aaatatctc tgtgtcaat tggggggcg caagcgggt cccattgaa cagaaaatc ctcgacgcg aatccagtc cttgaagct ttcggtaac gcgaaaaca acgcggaac aacaatagt tcccgtttc gggaaattc atggaagtg cacttcgat aaaaacttc cacgtggct ggaggacac atttcccac taccttctg gaaaagtcg cggatttgt tcgccgcaa ggcgacgaa cgcagctat cacattttt tacatgatg atggctgga gctcccgag gggttaaga caaaaactg gccttgact aaacccgac gattttaat tatctcaaa tcgggttgt acgcgatat ttcaccacc gccgccaat gagaaaaag ttaactccg ggccagaaa agcgcagat cattccaaa aacggtcct ttgaaggac attttaatc gacgatatt gccgatttt ctgaccctt gatggtgct ttatcacgg ttgggtttg agtgattct gaccggttt gacatctac tcgactgtg gcggcagtt ttgcacctt ggcaacatc gagtttgag gaaaaccca gaagataca agaggtggt tgcagagtg tcaccaagt aaagctaaa tcactcgaa attgcatct gggcttttg ggcattgac ccaactgag cttcgacaa gccttggaa gctcgagtc atgcaaagt agcagaggt ggggccaaa ggaacagtc atcatggtt ccgcttaaa gtttacgaa gcgaataat gctcgcgac gctctcgcg aaagctatc tacagcaac cttttcgat tacattgtg aatcgaatc aatcagagc attccgttc caaacctcc agctactac atcggtgtg ttagacata gccgggttt gagtttttc actgttaac agttttgag cagttttgt atcaactac tgtaacgaa aagctccaa cagttcttt aacgaagtg attttgaaa aacgagcaa gaactgtac aaacgtgaa ggtttgagc gtgcctgag gtgaacttc gtggataat caagactgc attaattta attgaaact aagagtaac ggaattctc caaatcctt gacgaagaa tccaaactg ccgaaacct tcctacagt cattttacg tcagaagtg cacaaacac tggcctaac gagttcagg attggttta ccgagatcg tccaaactg aaatcgcac cgtagtctt cgggacgat gaaggtttc ctaatccgg catttcgcc ggtgctgtg tgttatcaa accaaacaa ttcattgat aaaaacaat gacgccttg cacgcatca ctggaaggg atagtccaa gacagcaaa agcaagctg atccaaact cttttcgcc acatcaaat gcacaaaag gggaaactc acgtttata agcgtcggc aataaattc aaaacgcag ttgctcgaa ctgatggaa aagttgagg aagaatggg accaatttt atccgttgt atcaaaccg aataataaa atgctcgac aatcaattc gacggtgcc ttgtgtttg atgcaactg aagtgcctt ggcatgacc tcagttttg gagctcatg gagcatggg taccccagt cgggcccca tttaccgat ttgtacaac atgtacaaa gagtatctt ccgccagaa ttgaagaaa ctcaaccct aagacgttt tgtgaagca atgctccac agtttgaaa ctaaaagac cgggatttc aaattcgga gtcacccga gtgtttttc cggccggga aaattcgcc gaatttgat aaaatcatg aaatctgat cccgagaat ttgaaagcg atcatcgct gatgttaag aagttcctt gtcaagtcg cggtggacc aaagcacag ttctgtgcc ctcactgtt atcaaattg aaaaacaaa attatttac cggaaacgt gccctcgtt gtcgtacaa aagactgtg aaaggttac ttgactcga aaacgccac ggcccgagg attaaagct ttgaccaaa attaagagt cttgatggt aatttaaag aagatggaa gcgattgct gggcaattg aagaaagag aaagaagca acaactaat gaaattaac caacttaaa actgaaata agtgggacg attgggcgt attaagaag gaggataaa atggcagct tccgccctg gacggtctt tacaagcag ttggttgcg aaagttgac aagcaaatg gattctttg cagaagaag ttgctagaa cagaagaat gcggaagag caagcccga ttgaagaaa atccaggaa gaaatggaa cgtgagaag aagcgcaaa gaggaggaa gaacgtcga ttgagggaa gaagaagag aacaggaag aagaaggcc gaaatggaa gcccgccga aagaaggaa gaagaagaa cgcaaacgc caagaggaa gccgatcga agaacagcc gaacttctt caagcgcaa caagcaaaa gacgaactg gatgaacgc cgctatcag gaacaattg gagcaagaa cgccaagac cacgaactt gctctccgt ctagcacaa gaaagtaac ggacaactc gaagagagt cctcccatg attcgaaaa tcagaagct gtgaagaac caacaagcg gccctcgcc aaaggcaag tacgacctc accaagtgg aaatattcg gaactgcgc gacaccatc aacacttcc tgtgatatc gaactgctg gaagcttgc cgtcacgaa ttccaccgc cgcttgaag gtgtaccac gcctggaag gccaagaac cgcaaacgc accatcatg gacgagaac gaacgcgcc ccgcgctca gtcatggaa tcagccgcc aggacacca aagatccaa caaaaagca ccagttttg gacaataac tcccaacgg tacttccgc attcccttc gtacgtcca ggaggggat aacagtaaa aggggttgg tggtatgcg catttcgat ggccagtat gtagcaagg cagatcgag ctgcatccg gagaaggcg ccgattttg cttgttgca ggtaaagac gatatgcaa atgtgtgaa ctttcgttg gaagaaacg ggattgacg aggaaacga ggggcggaa attttggaa agcgagttt aataaggag tgggagaag cacggtgga gccaagtat gtgcgaccg gctgatcga aaacaatag taaagtaaa taagtttgt tagttaaag gggttttga taattttgc aaaaggtag ctttgcagt attaaatat acagctata tattgaaat gtaaacaat gataagtta ttgataata aattattta tcgttaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
