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Gene information
General information
TC000685 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7597187..7605179
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000685
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00152

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature differentiation incomplete - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000685 RA
      MDSVWARDP TEGFILARI SELLVEGAE VIPVNSKFP 
      KRVLPFSDI FKAQEDDVN KDYDDNCEM VFLNEGSLL 
      NNIRIRYYQ NKIYTYVAN ILIAVNPYK DIPQLYSPQ 
      TIKNYQGKS LGQMPPHVF AIADKAFRD MKTLKQSQS 
      IIVSGESGA GKTESTKHL LKYLCVNWG AQAGPIEQK 
      ILDANPVLE AFGNAKTTR NNNSSRFGK FMEVHFDKN 
      FHVAGGHIS HYLLEKSRI CSPQGDERS YHIFYMMMA 
      GAPEGLRQK LALTKPDDF NYLKSGCTR YFTTAANEK 
      KLTPGQKSA DHSKNGPLK DILIDDIAD FLTLDGALS 
      RLGLSDSDR FDIYSTVAA VLHLGNIEF EENPEDTRG 
      GCRVSPSKA KSLEIASGL LGIDPTELR QALEARVMQ 
      SSRGGAKGT VIMVPLKVY EANNARDAL AKAIYSNLF 
      DYIVNRINQ SIPFQTSSY YIGVLDIAG FEFFTVNSF 
      EQFCINYCN EKLQQFFNE VILKNEQEL YKREGLSVP 
      EVNFVDNQD CINLIETKS NGILQILDE ESKLPKPSY 
      SHFTSEVHK HWPNEFRIG LPRSSKLKS HRSLRDDEG 
      FLIRHFAGA VCYQTKQFI DKNNDALHA SLEGIVQDS 
      KSKLIQTLF ATSNAQKGK LTFISVGNK FKTQLLELM 
      EKLRKNGTN FIRCIKPNN KMLDNQFDG ALCLMQLKC 
      LGMTSVLEL MEHGYPSRA PFTDLYNMY KEYLPPELK 
      KLNPKTFCE AMLHSLKLK DRDFKFGVT RVFFRPGKF 
      AEFDKIMKS DPENLKAII ADVKKFLVK SRWTKAQFC 
      ALTVIKLKN KIIYRKRAL VVVQKTVKG YLTRKRHGP 
      RIKALTKIK SLDGNLKKM EAIAGQLKK EKEATTNEI 
      NQLKTEISG TIGRIKKED KMAASALDG LYKQLVAKV 
      DKQMDSLQK KLLEQKNAE EQARLKKIQ EEMEREKKR 
      KEEEERRLR EEEENRKKK AEMEARRKK EEEERKRQE 
      EADRRTAEL LQAQQAKDE LDERRYQEQ LEQERQDHE 
      LALRLAQES NGQLEESPP MIRKSEAVK NQQAALAKG 
      KYDLTKWKY SELRDTINT SCDIELLEA CRHEFHRRL 
      KVYHAWKAK NRKRTIMDE NERAPRSVM ESAARTPKI 
      QQKAPVLDN NSQRYFRIP FVRPGGDNS KRGWWYAHF 
      DGQYVARQI ELHPEKAPI LLVAGKDDM QMCELSLEE 
      TGLTRKRGA EILESEFNK EWEKHGGAK YVRPADRKQ
    CDS sequence
    • >TC000685 RA
      atggactcg gtttgggct cgggaccct accgaaggg 
      ttcatcctt gcccgaatt tccgaactt ttagtagag 
      ggtgctgaa gtaatcccc gtcaactcg aaattcccc 
      aaacgggtc ttgcctttc agcgacatt ttcaaagca 
      caagaagat gacgtaaat aaagactac gatgacaac 
      tgcgaaatg gttttccta aacgaaggt tcactcctc 
      aacaatatc cgtatcaga tattaccaa aataaaatc 
      tacacctac gtggcaaac atcctaatc gccgtcaac 
      ccctacaaa gacatcccc caactctac tccccccag 
      acaatcaaa aactaccaa ggcaaatct ttgggccaa 
      atgccaccc cacgttttc gcgatcgcc gacaaggct 
      tttcgcgat atgaaaacc ctgaagcaa tcccaatcg 
      attatagtt tccggagag tcaggagct ggcaaaacc 
      gaatccacc aaacatctc ctcaaatat ctctgtgtc 
      aattggggg gcgcaagcg ggtcccatt gaacagaaa 
      atcctcgac gcgaatcca gtccttgaa gctttcggt 
      aacgcgaaa acaacgcgg aacaacaat agttcccgt 
      ttcgggaaa ttcatggaa gtgcacttc gataaaaac 
      ttccacgtg gctggagga cacatttcc cactacctt 
      ctggaaaag tcgcggatt tgttcgccg caaggcgac 
      gaacgcagc tatcacatt ttttacatg atgatggct 
      ggagctccc gaggggtta agacaaaaa ctggccttg 
      actaaaccc gacgatttt aattatctc aaatcgggt 
      tgtacgcga tatttcacc accgccgcc aatgagaaa 
      aagttaact ccgggccag aaaagcgca gatcattcc 
      aaaaacggt cctttgaag gacatttta atcgacgat 
      attgccgat tttctgacc cttgatggt gctttatca 
      cggttgggt ttgagtgat tctgaccgg tttgacatc 
      tactcgact gtggcggca gttttgcac cttggcaac 
      atcgagttt gaggaaaac ccagaagat acaagaggt 
      ggttgcaga gtgtcacca agtaaagct aaatcactc 
      gaaattgca tctgggctt ttgggcatt gacccaact 
      gagcttcga caagccttg gaagctcga gtcatgcaa 
      agtagcaga ggtggggcc aaaggaaca gtcatcatg 
      gttccgctt aaagtttac gaagcgaat aatgctcgc 
      gacgctctc gcgaaagct atctacagc aaccttttc 
      gattacatt gtgaatcga atcaatcag agcattccg 
      ttccaaacc tccagctac tacatcggt gtgttagac 
      atagccggg tttgagttt ttcactgtt aacagtttt 
      gagcagttt tgtatcaac tactgtaac gaaaagctc 
      caacagttc tttaacgaa gtgattttg aaaaacgag 
      caagaactg tacaaacgt gaaggtttg agcgtgcct 
      gaggtgaac ttcgtggat aatcaagac tgcattaat 
      ttaattgaa actaagagt aacggaatt ctccaaatc 
      cttgacgaa gaatccaaa ctgccgaaa ccttcctac 
      agtcatttt acgtcagaa gtgcacaaa cactggcct 
      aacgagttc aggattggt ttaccgaga tcgtccaaa 
      ctgaaatcg caccgtagt cttcgggac gatgaaggt 
      ttcctaatc cggcatttc gccggtgct gtgtgttat 
      caaaccaaa caattcatt gataaaaac aatgacgcc 
      ttgcacgca tcactggaa gggatagtc caagacagc 
      aaaagcaag ctgatccaa actcttttc gccacatca 
      aatgcacaa aaggggaaa ctcacgttt ataagcgtc 
      ggcaataaa ttcaaaacg cagttgctc gaactgatg 
      gaaaagttg aggaagaat gggaccaat tttatccgt 
      tgtatcaaa ccgaataat aaaatgctc gacaatcaa 
      ttcgacggt gccttgtgt ttgatgcaa ctgaagtgc 
      cttggcatg acctcagtt ttggagctc atggagcat 
      gggtacccc agtcgggcc ccatttacc gatttgtac 
      aacatgtac aaagagtat cttccgcca gaattgaag 
      aaactcaac cctaagacg ttttgtgaa gcaatgctc 
      cacagtttg aaactaaaa gaccgggat ttcaaattc 
      ggagtcacc cgagtgttt ttccggccg ggaaaattc 
      gccgaattt gataaaatc atgaaatct gatcccgag 
      aatttgaaa gcgatcatc gctgatgtt aagaagttc 
      cttgtcaag tcgcggtgg accaaagca cagttctgt 
      gccctcact gttatcaaa ttgaaaaac aaaattatt 
      taccggaaa cgtgccctc gttgtcgta caaaagact 
      gtgaaaggt tacttgact cgaaaacgc cacggcccg 
      aggattaaa gctttgacc aaaattaag agtcttgat 
      ggtaattta aagaagatg gaagcgatt gctgggcaa 
      ttgaagaaa gagaaagaa gcaacaact aatgaaatt 
      aaccaactt aaaactgaa ataagtggg acgattggg 
      cgtattaag aaggaggat aaaatggca gcttccgcc 
      ctggacggt ctttacaag cagttggtt gcgaaagtt 
      gacaagcaa atggattct ttgcagaag aagttgcta 
      gaacagaag aatgcggaa gagcaagcc cgattgaag 
      aaaatccag gaagaaatg gaacgtgag aagaagcgc 
      aaagaggag gaagaacgt cgattgagg gaagaagaa 
      gagaacagg aagaagaag gccgaaatg gaagcccgc 
      cgaaagaag gaagaagaa gaacgcaaa cgccaagag 
      gaagccgat cgaagaaca gccgaactt cttcaagcg 
      caacaagca aaagacgaa ctggatgaa cgccgctat 
      caggaacaa ttggagcaa gaacgccaa gaccacgaa 
      cttgctctc cgtctagca caagaaagt aacggacaa 
      ctcgaagag agtcctccc atgattcga aaatcagaa 
      gctgtgaag aaccaacaa gcggccctc gccaaaggc 
      aagtacgac ctcaccaag tggaaatat tcggaactg 
      cgcgacacc atcaacact tcctgtgat atcgaactg 
      ctggaagct tgccgtcac gaattccac cgccgcttg 
      aaggtgtac cacgcctgg aaggccaag aaccgcaaa 
      cgcaccatc atggacgag aacgaacgc gccccgcgc 
      tcagtcatg gaatcagcc gccaggaca ccaaagatc 
      caacaaaaa gcaccagtt ttggacaat aactcccaa 
      cggtacttc cgcattccc ttcgtacgt ccaggaggg 
      gataacagt aaaaggggt tggtggtat gcgcatttc 
      gatggccag tatgtagca aggcagatc gagctgcat 
      ccggagaag gcgccgatt ttgcttgtt gcaggtaaa 
      gacgatatg caaatgtgt gaactttcg ttggaagaa 
      acgggattg acgaggaaa cgaggggcg gaaattttg 
      gaaagcgag tttaataag gagtgggag aagcacggt 
      ggagccaag tatgtgcga ccggctgat cgaaaacaa 
      tag
    mRNA sequence
    • >TC000685 RA
      aaagcctta aaagtgtgg taaatcgcc caccaatcg 
      aaatctctt tgtattaca ttcgaactc aagcgcttt 
      ccatgttgt ttcgctgag acgtgcgca ctcggcgga 
      cattttaat tcccattta aaaattctt cgctactcg 
      cggtgtgtg gaacggaaa gagcaaaca caaacacag 
      tgctgggtc atatgtgcg caaggtgtt cgtcaaggt 
      ggttttatc gcaacttta cggtagttt gttaataaa 
      actaggagg atttagcgg aaaacagag tgaaacatg 
      gactcggtt tgggctcgg gaccctacc gaagggttc 
      atccttgcc cgaatttcc gaactttta gtagagggt 
      gctgaagta atccccgtc aactcgaaa ttccccaaa 
      cgggtcttg cctttcagc gacattttc aaagcacaa 
      gaagatgac gtaaataaa gactacgat gacaactgc 
      gaaatggtt ttcctaaac gaaggttca ctcctcaac 
      aatatccgt atcagatat taccaaaat aaaatctac 
      acctacgtg gcaaacatc ctaatcgcc gtcaacccc 
      tacaaagac atcccccaa ctctactcc ccccagaca 
      atcaaaaac taccaaggc aaatctttg ggccaaatg 
      ccaccccac gttttcgcg atcgccgac aaggctttt 
      cgcgatatg aaaaccctg aagcaatcc caatcgatt 
      atagtttcc ggagagtca ggagctggc aaaaccgaa 
      tccaccaaa catctcctc aaatatctc tgtgtcaat 
      tggggggcg caagcgggt cccattgaa cagaaaatc 
      ctcgacgcg aatccagtc cttgaagct ttcggtaac 
      gcgaaaaca acgcggaac aacaatagt tcccgtttc 
      gggaaattc atggaagtg cacttcgat aaaaacttc 
      cacgtggct ggaggacac atttcccac taccttctg 
      gaaaagtcg cggatttgt tcgccgcaa ggcgacgaa 
      cgcagctat cacattttt tacatgatg atggctgga 
      gctcccgag gggttaaga caaaaactg gccttgact 
      aaacccgac gattttaat tatctcaaa tcgggttgt 
      acgcgatat ttcaccacc gccgccaat gagaaaaag 
      ttaactccg ggccagaaa agcgcagat cattccaaa 
      aacggtcct ttgaaggac attttaatc gacgatatt 
      gccgatttt ctgaccctt gatggtgct ttatcacgg 
      ttgggtttg agtgattct gaccggttt gacatctac 
      tcgactgtg gcggcagtt ttgcacctt ggcaacatc 
      gagtttgag gaaaaccca gaagataca agaggtggt 
      tgcagagtg tcaccaagt aaagctaaa tcactcgaa 
      attgcatct gggcttttg ggcattgac ccaactgag 
      cttcgacaa gccttggaa gctcgagtc atgcaaagt 
      agcagaggt ggggccaaa ggaacagtc atcatggtt 
      ccgcttaaa gtttacgaa gcgaataat gctcgcgac 
      gctctcgcg aaagctatc tacagcaac cttttcgat 
      tacattgtg aatcgaatc aatcagagc attccgttc 
      caaacctcc agctactac atcggtgtg ttagacata 
      gccgggttt gagtttttc actgttaac agttttgag 
      cagttttgt atcaactac tgtaacgaa aagctccaa 
      cagttcttt aacgaagtg attttgaaa aacgagcaa 
      gaactgtac aaacgtgaa ggtttgagc gtgcctgag 
      gtgaacttc gtggataat caagactgc attaattta 
      attgaaact aagagtaac ggaattctc caaatcctt 
      gacgaagaa tccaaactg ccgaaacct tcctacagt 
      cattttacg tcagaagtg cacaaacac tggcctaac 
      gagttcagg attggttta ccgagatcg tccaaactg 
      aaatcgcac cgtagtctt cgggacgat gaaggtttc 
      ctaatccgg catttcgcc ggtgctgtg tgttatcaa 
      accaaacaa ttcattgat aaaaacaat gacgccttg 
      cacgcatca ctggaaggg atagtccaa gacagcaaa 
      agcaagctg atccaaact cttttcgcc acatcaaat 
      gcacaaaag gggaaactc acgtttata agcgtcggc 
      aataaattc aaaacgcag ttgctcgaa ctgatggaa 
      aagttgagg aagaatggg accaatttt atccgttgt 
      atcaaaccg aataataaa atgctcgac aatcaattc 
      gacggtgcc ttgtgtttg atgcaactg aagtgcctt 
      ggcatgacc tcagttttg gagctcatg gagcatggg 
      taccccagt cgggcccca tttaccgat ttgtacaac 
      atgtacaaa gagtatctt ccgccagaa ttgaagaaa 
      ctcaaccct aagacgttt tgtgaagca atgctccac 
      agtttgaaa ctaaaagac cgggatttc aaattcgga 
      gtcacccga gtgtttttc cggccggga aaattcgcc 
      gaatttgat aaaatcatg aaatctgat cccgagaat 
      ttgaaagcg atcatcgct gatgttaag aagttcctt 
      gtcaagtcg cggtggacc aaagcacag ttctgtgcc 
      ctcactgtt atcaaattg aaaaacaaa attatttac 
      cggaaacgt gccctcgtt gtcgtacaa aagactgtg 
      aaaggttac ttgactcga aaacgccac ggcccgagg 
      attaaagct ttgaccaaa attaagagt cttgatggt 
      aatttaaag aagatggaa gcgattgct gggcaattg 
      aagaaagag aaagaagca acaactaat gaaattaac 
      caacttaaa actgaaata agtgggacg attgggcgt 
      attaagaag gaggataaa atggcagct tccgccctg 
      gacggtctt tacaagcag ttggttgcg aaagttgac 
      aagcaaatg gattctttg cagaagaag ttgctagaa 
      cagaagaat gcggaagag caagcccga ttgaagaaa 
      atccaggaa gaaatggaa cgtgagaag aagcgcaaa 
      gaggaggaa gaacgtcga ttgagggaa gaagaagag 
      aacaggaag aagaaggcc gaaatggaa gcccgccga 
      aagaaggaa gaagaagaa cgcaaacgc caagaggaa 
      gccgatcga agaacagcc gaacttctt caagcgcaa 
      caagcaaaa gacgaactg gatgaacgc cgctatcag 
      gaacaattg gagcaagaa cgccaagac cacgaactt 
      gctctccgt ctagcacaa gaaagtaac ggacaactc 
      gaagagagt cctcccatg attcgaaaa tcagaagct 
      gtgaagaac caacaagcg gccctcgcc aaaggcaag 
      tacgacctc accaagtgg aaatattcg gaactgcgc 
      gacaccatc aacacttcc tgtgatatc gaactgctg 
      gaagcttgc cgtcacgaa ttccaccgc cgcttgaag 
      gtgtaccac gcctggaag gccaagaac cgcaaacgc 
      accatcatg gacgagaac gaacgcgcc ccgcgctca 
      gtcatggaa tcagccgcc aggacacca aagatccaa 
      caaaaagca ccagttttg gacaataac tcccaacgg 
      tacttccgc attcccttc gtacgtcca ggaggggat 
      aacagtaaa aggggttgg tggtatgcg catttcgat 
      ggccagtat gtagcaagg cagatcgag ctgcatccg 
      gagaaggcg ccgattttg cttgttgca ggtaaagac 
      gatatgcaa atgtgtgaa ctttcgttg gaagaaacg 
      ggattgacg aggaaacga ggggcggaa attttggaa 
      agcgagttt aataaggag tgggagaag cacggtgga 
      gccaagtat gtgcgaccg gctgatcga aaacaatag 
      taaagtaaa taagtttgt tagttaaag gggttttga 
      taattttgc aaaaggtag ctttgcagt attaaatat 
      acagctata tattgaaat gtaaacaat gataagtta 
      ttgataata aattattta tcgttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY