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Gene information
General information
TC000678 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7630729..7632179
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000678
    iBeetle screen iB_00151

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000678 RA
      MTQLFELIA ATLWFWTFS IGFLVSIIS SVTILFCPT 
      LRWVLLAYL LWIWYDRKT SERGGRPIP WVRTFLWWR 
      LMKNYFSHK LISNENDEF DPKKNYLFA CYPHGMLPV 
      GTFASFVDG FRSSFPKHE AYTTVLNIQ FWMPVIREL 
      FLSIGAVSV SGPSIKYLL SNEKGGNIV SILIGGADE 
      SKYSKPGKY KIILNKRKG FVKMALQTG APLVPVFSF 
      GETDVFDQV DFPGFGCIR DLVKNTLQV GLVIPKGKF 
      FICPQRVPV VTVVGSPIN VTKTANPTN EQIDELHRQ 
      FVDGIKTLF DQYKHRYVK NPDKTVLEI V
    CDS sequence
    • >TC000678 RA
      atgactcaa ctgttcgaa ctaatcgcc gccacacta 
      tggttttgg acgttttca atcggtttc ctggtctca 
      attataagc tccgtcaca attttattt tgcccaacg 
      ctgcggtgg gtgctcctg gcgtacctc ctctggatc 
      tggtacgac cgcaagacc agtgaacga ggcggcaga 
      cccatccca tgggtgcgc acgttcctc tggtggcgc 
      ctcatgaag aactacttc agccacaag ctaatatca 
      aacgagaac gacgaattc gaccccaag aaaaactac 
      ttattcgcg tgctacccc catggaatg ctcccagtg 
      ggcaccttt gcctcgttc gtggacggc ttcaggagc 
      agtttcccc aaacacgaa gcctacacc acggtactc 
      aacatccag ttctggatg ccggtgata agagaactc 
      ttcttatca atcggggct gtctcggta tcaggcccc 
      tcaatcaag tatttacta agtaatgaa aaaggcggc 
      aatattgta tcaattcta atcggcggt gccgacgaa 
      tctaagtac agcaaaccg gggaaatac aaaatcatt 
      ttaaataaa aggaaaggt ttcgtgaaa atggccctc 
      caaacgggg gcgcccttg gtccccgtg ttttcgttc 
      ggggaaacg gacgttttc gaccaagtg gacttcccc 
      gggtttggt tgcatcaga gatttggtc aaaaacacg 
      ctgcaggtt gggttagtt ataccaaaa gggaagttt 
      ttcatttgt ccacaacga gtccctgtc gtgaccgtc 
      gttgggtcg ccgattaat gtcacgaaa accgcaaat 
      cccaccaat gaacaaatc gatgagctg caccgacaa 
      tttgtcgat ggtattaag acacttttc gaccaatac 
      aagcacaga tacgtcaaa aatccggac aagactgtt 
      ttggagatt gtttaa
    mRNA sequence
    • >TC000678 RA
      cgtcgcacc tcgaagtaa aatgttcag ttttgttgt 
      ttacacacc gaggtgttc ttcaggtca ctgtccttg 
      aaagatgtg cacagtaag cacaacctg gtgagtcgt 
      ggttgctca agaacagtg aaatcgtat tttttatcg 
      aaatattat caccatgac tcaactgtt cgaactaat 
      cgccgccac actatggtt ttggacgtt ttcaatcgg 
      tttcctggt ctcaattat aagctccgt cacaatttt 
      attttgccc aacgctgcg gtgggtgct cctggcgta 
      cctcctctg gatctggta cgaccgcaa gaccagtga 
      acgaggcgg cagacccat cccatgggt gcgcacgtt 
      cctctggtg gcgcctcat gaagaacta cttcagcca 
      caagctaat atcaaacga gaacgacga attcgaccc 
      caagaaaaa ctacttatt cgcgtgcta cccccatgg 
      aatgctccc agtgggcac ctttgcctc gttcgtgga 
      cggcttcag gagcagttt ccccaaaca cgaagccta 
      caccacggt actcaacat ccagttctg gatgccggt 
      gataagaga actcttctt atcaatcgg ggctgtctc 
      ggtatcagg cccctcaat caagtattt actaagtaa 
      tgaaaaagg cggcaatat tgtatcaat tctaatcgg 
      cggtgccga cgaatctaa gtacagcaa accggggaa 
      atacaaaat cattttaaa taaaaggaa aggtttcgt 
      gaaaatggc cctccaaac gggggcgcc cttggtccc 
      cgtgttttc gttcgggga aacggacgt tttcgacca 
      agtggactt ccccgggtt tggttgcat cagagattt 
      ggtcaaaaa cacgctgca ggttgggtt agttatacc 
      aaaagggaa gtttttcat ttgtccaca acgagtccc 
      tgtcgtgac cgtcgttgg gtcgccgat taatgtcac 
      gaaaaccgc aaatcccac caatgaaca aatcgatga 
      gctgcaccg acaatttgt cgatggtat taagacact 
      tttcgacca atacaagca cagatacgt caaaaatcc 
      ggacaagac tgttttgga gattgttta attttgatt 
      ttgcaggtt ttcgggggt tttgtgggt taattgatt 
      ttgttaatt tatttttta ttaaaaatg tgttggtat 
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