| General information | |||
| TC000642 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 8601251..8607435 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC000642 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00149 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC000642 RA
MHCKQIKLG DVWRTCNKI RAAVFRTNL NLWEYFCHL DPNKNSIIS ESQFISVFT GHLQAIIGL SDQEISELA DYFRIQDGR ILYTQLCEV IYDSVPDFS QNAPLVTGL EWEDPLHVN RLSISEERR LNLLLTKIA CVVNMRNLL LRPYFQDYE LISKNNGTV TIGHFARVL DYLKILVSP DDFTLLLKK YLKDSYTVN YVAFIAAID EIVKYLDQH GVMDLSGDV LSLFPGRII NAELPKLPR PEIGKQLAA SIFGKQSIF HSALNEPKQ TQDLVATIK RIQRHVLEN RLRVSEFFK DFDALNSGR LTVSQFHRG LDMLGISGI QRLYLSLPE IEAIMIQYR DPCDPTRVC WKTFEDDID QVFTTKELE KYPCLCVDA PPREIVELP RMGGKDWQK VNPASRELC EEAVDKVKQ RVTKRRIML KPSFRDYDK HNNGYVSRS QMRQCLLSN GILLSDEEL YALEERFNN EVGFNYFWF LREVEPKSC EDRCPGAIH HEVCGEEYV KKPVPRAEQ DIVLIMAKI KGKVVRERI RVMEFLRDF DKCNQSVIS REDFKRGLS VCRFDLTEN EMETLMEVF ASPLRRECV DYRRFSDVV EEAFTQQSL ERAPLIVPL QHIPTRDGE RNFLNFDER VLLSSAMNK LSKKPEMEL NLIQIFQDY DKTNCGTVS GEHFERALS LRGLLNIIT RQEFDAVCK CFSFERGLR DEIDYRAFI KGLNILYNI NKYKPF
- >TC000642 RA
atgcattgc aaacaaata aaattaggt gatgtctgg cgcacttgt aataaaatc cgcgcagcg gtctttcgg acaaatcta aatctttgg gagtacttc tgtcatctc gacccgaac aaaaattcc ataatttct gagtcacag tttatttca gttttcacc ggtcatttg caggcgatt attggtctc tcggaccaa gaaatttca gaacttgcg gattatttc agaattcag gatggtcgt atcctgtat acgcaactg tgcgaagtt atttatgac agcgtgcct gatttttca caaaatgcc cccttagtc actggtctt gagtgggaa gaccctctc catgtaaac cgactgagc ataagtgaa gaaagacgc ttgaatctt cttctaact aaaattgca tgtgttgtg aacatgaga aatttgctg ctgaggccg tatttccaa gactacgaa cttatttct aaaaacaac ggaactgta acaatcggt catttcgct cgagttttg gactattta aaaattcta gtctcgcca gacgacttt actctctta ttgaaaaaa tacctgaag gacagttac acagtaaat tatgtcgca tttattgcg gcaatcgac gagattgta aaatacctg gaccagcac ggcgtcatg gacctgagt ggcgacgtc ctgagccta tttccggga agaataatc aacgcagaa ctgcccaaa ctgccgcga cccgaaatc ggcaaacaa ctagctgcg agcattttc ggcaaacaa agcatcttt cattccgcg ttaaacgaa ccgaaacaa acgcaagac ttggtcgct acaattaaa cgaattcaa agacacgtt ttggaaaat agattacgg gtgtcggaa tttttcaaa gactttgat gcgctaaat agtggacgc ttgaccgtg tcgcagttc caccgaggg ctggacatg ctggggatc agtggtatc cagagactt tacttgtct ttgcctgaa attgaggcc ataatgata caatatcgc gacccctgt gaccccact cgagtgtgt tggaagacg tttgaagac gatattgac caggttttc actaccaaa gagctggag aagtatccg tgtttgtgc gtggatgcg cccccgcgt gaaattgtc gagttgccc agaatgggg ggcaaagac tggcaaaaa gtaaatccc gcctccaga gaattgtgc gaagaagcg gttgataaa gtcaagcaa agagttacg aaaagacga atcatgtta aaaccatcc tttagggac tatgacaag cacaacaat ggttacgtt tcgcgttcg caaatgcgc cagtgttta ctgtctaat ggtattctc ttgtccgat gaagagttg tatgctttg gaagagcgt tttaataac gaggttggt ttcaactac ttctggttt ctgagggag gttgagccg aagagttgc gaggaccgc tgtcctggt gccatccac cacgaggtc tgtggcgaa gaatacgtg aaaaagccc gtgcctcgg gccgagcaa gacattgtc ctgattatg gccaaaatc aagggcaag gttgtgcgt gagcgaatc cgagtgatg gagtttttg cgtgatttt gacaagtgc aaccaaagt gttatttct cgtgaagat ttcaagcgg ggtttgtcg gtttgtcgc ttcgatttg accgaaaac gagatggaa acgctgatg gaagttttt gcctcgcct ttgaggcgc gaatgtgtg gactatcgc cggttttcg gacgtggtt gaggaggct ttcactcag caaagcctg gagcgtgct cctttaatc gtccccctg cagcatatc ccgaccagg gacggcgaa cggaatttc ctcaatttc gacgaaaga gtgctgctg tccagcgca atgaataag ctatcgaaa aagcccgaa atggagctc aatttgatt caaatcttc caggattat gacaagacc aactgtggg acggtttcg ggcgagcac ttcgaaagg gcgctgtcg ctgcggggc cttcttaac atcatcacg agacaggaa ttcgacgct gtttgcaaa tgcttcagt ttcgagagg gggctgcgg gacgagatc gactaccgg gcgttcatt aaggggctc aacatcctc tacaatata aataaatac aaacctttc tga
- >TC000642 RA
tagtgtgca gcaaaaatg cattgcaaa caaataaaa ttaggtgat gtctggcgc acttgtaat aaaatccgc gcagcggtc tttcggaca aatctaaat ctttgggag tacttctgt catctcgac ccgaacaaa aattccata atttctgag tcacagttt atttcagtt ttcaccggt catttgcag gcgattatt ggtctctcg gaccaagaa atttcagaa cttgcggat tatttcaga attcaggat ggtcgtatc ctgtatacg caactgtgc gaagttatt tatgacagc gtgcctgat ttttcacaa aatgccccc ttagtcact ggtcttgag tgggaagac cctctccat gtaaaccga ctgagcata agtgaagaa agacgcttg aatcttctt ctaactaaa attgcatgt gttgtgaac atgagaaat ttgctgctg aggccgtat ttccaagac tacgaactt atttctaaa aacaacgga actgtaaca atcggtcat ttcgctcga gttttggac tatttaaaa attctagtc tcgccagac gactttact ctcttattg aaaaaatac ctgaaggac agttacaca gtaaattat gtcgcattt attgcggca atcgacgag attgtaaaa tacctggac cagcacggc gtcatggac ctgagtggc gacgtcctg agcctattt ccgggaaga ataatcaac gcagaactg cccaaactg ccgcgaccc gaaatcggc aaacaacta gctgcgagc attttcggc aaacaaagc atctttcat tccgcgtta aacgaaccg aaacaaacg caagacttg gtcgctaca attaaacga attcaaaga cacgttttg gaaaataga ttacgggtg tcggaattt ttcaaagac tttgatgcg ctaaatagt ggacgcttg accgtgtcg cagttccac cgagggctg gacatgctg gggatcagt ggtatccag agactttac ttgtctttg cctgaaatt gaggccata atgatacaa tatcgcgac ccctgtgac cccactcga gtgtgttgg aagacgttt gaagacgat attgaccag gttttcact accaaagag ctggagaag tatccgtgt ttgtgcgtg gatgcgccc ccgcgtgaa attgtcgag ttgcccaga atggggggc aaagactgg caaaaagta aatcccgcc tccagagaa ttgtgcgaa gaagcggtt gataaagtc aagcaaaga gttacgaaa agacgaatc atgttaaaa ccatccttt agggactat gacaagcac aacaatggt tacgtttcg cgttcgcaa atgcgccag tgtttactg tctaatggt attctcttg tccgatgaa gagttgtat gctttggaa gagcgtttt aataacgag gttggtttc aactacttc tggtttctg agggaggtt gagccgaag agttgcgag gaccgctgt cctggtgcc atccaccac gaggtctgt ggcgaagaa tacgtgaaa aagcccgtg cctcgggcc gagcaagac attgtcctg attatggcc aaaatcaag ggcaaggtt gtgcgtgag cgaatccga gtgatggag tttttgcgt gattttgac aagtgcaac caaagtgtt atttctcgt gaagatttc aagcggggt ttgtcggtt tgtcgcttc gatttgacc gaaaacgag atggaaacg ctgatggaa gtttttgcc tcgcctttg aggcgcgaa tgtgtggac tatcgccgg ttttcggac gtggttgag gaggctttc actcagcaa agcctggag cgtgctcct ttaatcgtc cccctgcag catatcccg accagggac ggcgaacgg aatttcctc aatttcgac gaaagagtg ctgctgtcc agcgcaatg aataagcta tcgaaaaag cccgaaatg gagctcaat ttgattcaa atcttccag gattatgac aagaccaac tgtgggacg gtttcgggc gagcacttc gaaagggcg ctgtcgctg cggggcctt cttaacatc atcacgaga caggaattc gacgctgtt tgcaaatgc ttcagtttc gagaggggg ctgcgggac gagatcgac taccgggcg ttcattaag gggctcaac atcctctac aatataaat aaatacaaa cctttctga aaatatcgc gggcaaata tattgatat tattcgatt ttgtggctg attattccg tttggttgt tttccgatg gcgtcattg taagtgatt tgtcccggg agaggtgtg caagccgtt cgttgggtc ttgatgcaa taaatgaac caaattaga
