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Gene information
General information
TC000641 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8629199..8633865
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000641
    iBeetle screen iB_00148

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    remarks: earlier than schedule, adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Pupal morphology
    remarks: earlier than schedule,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000641 RA
      MTAVEQPCY TLINVPTDT EPYNEMQLK IDLEKGDVK 
      QKVEALKKT IHMILAGER LPPGLLMTI IRYVLPLQD 
      HTAKKLLLI FWEIVPKTS PDGKLLQEM ILVCDAYRK 
      DLQHPNEFL RGSTLRFLC KLKEPELLE PLMPSIRAC 
      LEHRHSYVR RNAVLAIFT IYRNFEFLI PDAPELISN 
      YLDGEQDMS CKRNAFLML LHADQERAL SYLASCLDQ 
      VTSFGDILQ LVIVELIYK VCHSNPSER SRFIRCIYN 
      LLNSSSPAV RYEAAGTLV TLSTAPTAI KAAASCYIE 
      LIIKESDNN VKLIVLDRL IALKDHPTH ERVLQDLVM 
      DILRVLSSP DLEVRKKTL NLAMDLVSS RNIEEMVLV 
      LKKEVAKTH DVEHEDTGK YRQLLVRTL HSCCIRFPD 
      VAATVIPVL VEFLSDTNE LAAVDVLIF VREAIQKFD 
      NLQPLIIEK LLEAFPNIK SVKIHRAAL WILGEYATT 
      VADIQSVLK AINQTLGEG PLLEAEQRL VAGDSEEVT 
      QPNPTQASS TLVTSDGTY ATQSAFNTV QKSTKEKRP 
      PLRQYLIDG DFFIGAAIA STLTKLALR YGSSVKNAV 
      ERNRFDAEV MMIMAGIIH LGKSGLPTK AMTNDDKDH 
      VLFCLRVLS DRQPSIIKV FTDLCRQAL NDMLVAKEI 
      EEALNQKAK EKAGNVIQA DDPINFMQL QADKSGELG 
      ENVFETSLS QALVGGRVG GSDTSTGVN KLNKVTQLT 
      GFSDPVYAE AYVHVNQYD IVLDVLIVN QTNDTLQNC 
      TLELATLGD LKLVEKPQP VVLAPKDFC NIKANVKVA 
      STENGIIFG NIVYDVTGA ASDRNVVVL DDIHIDIMD 
      YIVPATCSD SEFRQMWAE FEWENKVSV NTTLTDLAE 
      YLKHLIKST NMKCLTPEK ALSGQCGFM AANMYAKSI 
      FGEDALANL SIEKPFNKP DAPVTGHIR IRAKSQGMA 
      LSLGDKINM TQKGQQSKV IAA
    CDS sequence
    • >TC000641 RA
      atgactgca gtcgaacag ccctgttac accctcata 
      aacgtcccc accgacaca gaaccctac aatgagatg 
      caacttaag atagaccta gaaaagggc gacgtcaaa 
      caaaaagtc gaagctttg aaaaagacc attcacatg 
      atcctggcc ggcgagcgc ctgcccccg ggcctcctc 
      atgaccata atccgctac gtcctccct ttgcaagac 
      cacaccgcc aaaaaacta ctcctcatc ttctgggag 
      atcgtccct aagacctcc cccgacggc aaactcctc 
      caggaaatg atcctcgtc tgcgacgcc taccgcaag 
      gatttgcaa cacccgaac gaatttctc aggggctca 
      actttgcgc ttcttgtgc aaattgaaa gagcccgag 
      cttttggag cccctgatg ccctcgatc cgggcctgt 
      ctggagcac agacactcc tacgtgcgg cgcaatgcc 
      gtccttgcg attttcaca atttaccga aatttcgaa 
      tttttgatc ccggacgct ccagagttg atttcgaat 
      tacttggac ggggagcaa gacatgtcg tgtaagcgg 
      aacgctttc ctcatgctg ctccacgca gatcaggag 
      cgcgccttg agctatttg gcctcgtgc ttggaccaa 
      gtgacctcc ttcggggac atcctccag ttggttatt 
      gtcgaattg atttacaaa gtttgccac agcaatccg 
      tctgaacgg tccaggttc atccgctgt atttacaat 
      ttgctcaat tcgagcagc cccgctgtg cggtatgag 
      gctgcgggg actttggtc acgctctcc acagccccc 
      acggcgatt aaagcggcc gccagttgc tacattgag 
      ctcataatc aaggaaagc gacaacaat gtcaagctc 
      attgttttg gaccgtctg atcgctctc aaagaccac 
      ccaactcac gaaagggtt cttcaagac ttggttatg 
      gatattttg agggttttg tcgagtcct gatttggag 
      gtgcgcaaa aagactctt aatctggcg atggatttg 
      gtttcgtcg cgaaatatc gaagaaatg gtcttggtt 
      ttgaagaaa gaagttgcg aaaacgcac gatgttgag 
      catgaagat accggaaaa tatagacag ttgctagtc 
      cggactttg cattcttgt tgtattcga tttccggat 
      gttgcagca actgttatt ccagttttg gtcgaattc 
      ttatctgat acaaatgag cttgcagcc gttgatgtt 
      ctgatcttc gtcagagag gcgattcag aaattcgat 
      aatttgcag ccgctcatc attgagaaa ctcctcgaa 
      gctttcccc aacataaaa tcggtgaaa atccaccga 
      gccgccctt tggatttta ggcgaatac gcaacaact 
      gtggccgac atccagtcc gtacttaaa gccatcaac 
      caaacccta ggcgagggc ccccttctg gaagcggag 
      caacgtcta gtggccgga gattcggaa gaagtcacc 
      caaccgaac ccaacacaa gcttccagc accctcgtc 
      acttctgac ggaacttac gccacccaa tcagccttc 
      aacacggtt cagaaaagc acaaaagag aagcggccc 
      ccacttcgc cagtatcta atcgacggc gatttcttt 
      atcggggct gcgatcgcc tcaactttg accaaactg 
      gctctaagg tacggcagt tcggttaaa aatgcggtt 
      gaaaggaat cggtttgat gccgaagtt atgatgatc 
      atggccggg attattcac ttggggaag tcgggtttg 
      ccaactaag gccatgacc aatgatgat aaggaccac 
      gttttattc tgcttgcgg gtgctttcg gaccggcag 
      ccgagcatt atcaaggtt tttaccgac ttgtgccga 
      caggcactt aacgacatg ttagtcgcc aaagaaatt 
      gaagaagca ctcaatcag aaagcgaaa gaaaaggct 
      ggtaatgtc atacaagcc gacgatccg atcaatttt 
      atgcaatta caagccgac aagtcgggc gaattgggt 
      gaaaacgtc ttcgaaacg tccttgtca caagccttg 
      gttgggggc cgagtcggc ggcagtgac acaagcact 
      ggggttaac aagctgaac aaagtgact caattgacc 
      ggcttttca gacccggtg tatgccgaa gcctacgtc 
      catgtcaac cagtatgat attgttttg gacgttttg 
      atagtcaac cagaccaac gatacgtta caaaactgc 
      acccttgag ttggcaact ctcggcgat ttgaaactc 
      gtggaaaaa ccgcagccg gtggttctg gcgcccaaa 
      gacttctgc aacattaag gccaacgtg aaagtggcc 
      tcgaccgaa aacgggatt attttcggg aacatagtt 
      tatgacgtc acgggagca gcgtccgac cgaaacgtg 
      gtggtcctg gacgacatc catattgat attatggat 
      tacatagtc ccggccact tgtagcgac tcggagttc 
      aggcaaatg tgggccgag ttcgagtgg gagaataag 
      gtttcggtt aatacgacc ttaaccgac cttgcggaa 
      tatttgaag cacttgatc aaaagcacc aacatgaag 
      tgtttgact ccggagaag gccctgtca gggcagtgc 
      ggtttcatg gccgccaat atgtacgca aaatcaatc 
      tttggagag gacgctctc gctaatttg agtattgaa 
      aaaccgttc aacaaacct gatgcaccc gttactgga 
      catatcagg atcagggcc aagagtcag gggatggct 
      ctgagtttg ggtgacaag atcaatatg acacagaag 
      gggcaacag agtaaagtt atcgctgca taa
    mRNA sequence
    • >TC000641 RA
      gttgacacg tcgttgaca tttaattcc aaaatatta 
      acattacgt gttttgtgt taaataagt gttttttct 
      cagttaaag tggtaaatc tccacaatg actgcagtc 
      gaacagccc tgttacacc ctcataaac gtccccacc 
      gacacagaa ccctacaat gagatgcaa cttaagata 
      gacctagaa aagggcgac gtcaaacaa aaagtcgaa 
      gctttgaaa aagaccatt cacatgatc ctggccggc 
      gagcgcctg cccccgggc ctcctcatg accataatc 
      cgctacgtc ctccctttg caagaccac accgccaaa 
      aaactactc ctcatcttc tgggagatc gtccctaag 
      acctccccc gacggcaaa ctcctccag gaaatgatc 
      ctcgtctgc gacgcctac cgcaaggat ttgcaacac 
      ccgaacgaa tttctcagg ggctcaact ttgcgcttc 
      ttgtgcaaa ttgaaagag cccgagctt ttggagccc 
      ctgatgccc tcgatccgg gcctgtctg gagcacaga 
      cactcctac gtgcggcgc aatgccgtc cttgcgatt 
      ttcacaatt taccgaaat ttcgaattt ttgatcccg 
      gacgctcca gagttgatt tcgaattac ttggacggg 
      gagcaagac atgtcgtgt aagcggaac gctttcctc 
      atgctgctc cacgcagat caggagcgc gccttgagc 
      tatttggcc tcgtgcttg gaccaagtg acctccttc 
      ggggacatc ctccagttg gttattgtc gaattgatt 
      tacaaagtt tgccacagc aatccgtct gaacggtcc 
      aggttcatc cgctgtatt tacaatttg ctcaattcg 
      agcagcccc gctgtgcgg tatgaggct gcggggact 
      ttggtcacg ctctccaca gcccccacg gcgattaaa 
      gcggccgcc agttgctac attgagctc ataatcaag 
      gaaagcgac aacaatgtc aagctcatt gttttggac 
      cgtctgatc gctctcaaa gaccaccca actcacgaa 
      agggttctt caagacttg gttatggat attttgagg 
      gttttgtcg agtcctgat ttggaggtg cgcaaaaag 
      actcttaat ctggcgatg gatttggtt tcgtcgcga 
      aatatcgaa gaaatggtc ttggttttg aagaaagaa 
      gttgcgaaa acgcacgat gttgagcat gaagatacc 
      ggaaaatat agacagttg ctagtccgg actttgcat 
      tcttgttgt attcgattt ccggatgtt gcagcaact 
      gttattcca gttttggtc gaattctta tctgataca 
      aatgagctt gcagccgtt gatgttctg atcttcgtc 
      agagaggcg attcagaaa ttcgataat ttgcagccg 
      ctcatcatt gagaaactc ctcgaagct ttccccaac 
      ataaaatcg gtgaaaatc caccgagcc gccctttgg 
      attttaggc gaatacgca acaactgtg gccgacatc 
      cagtccgta cttaaagcc atcaaccaa accctaggc 
      gagggcccc cttctggaa gcggagcaa cgtctagtg 
      gccggagat tcggaagaa gtcacccaa ccgaaccca 
      acacaagct tccagcacc ctcgtcact tctgacgga 
      acttacgcc acccaatca gccttcaac acggttcag 
      aaaagcaca aaagagaag cggccccca cttcgccag 
      tatctaatc gacggcgat ttctttatc ggggctgcg 
      atcgcctca actttgacc aaactggct ctaaggtac 
      ggcagttcg gttaaaaat gcggttgaa aggaatcgg 
      tttgatgcc gaagttatg atgatcatg gccgggatt 
      attcacttg gggaagtcg ggtttgcca actaaggcc 
      atgaccaat gatgataag gaccacgtt ttattctgc 
      ttgcgggtg ctttcggac cggcagccg agcattatc 
      aaggttttt accgacttg tgccgacag gcacttaac 
      gacatgtta gtcgccaaa gaaattgaa gaagcactc 
      aatcagaaa gcgaaagaa aaggctggt aatgtcata 
      caagccgac gatccgatc aattttatg caattacaa 
      gccgacaag tcgggcgaa ttgggtgaa aacgtcttc 
      gaaacgtcc ttgtcacaa gccttggtt gggggccga 
      gtcggcggc agtgacaca agcactggg gttaacaag 
      ctgaacaaa gtgactcaa ttgaccggc ttttcagac 
      ccggtgtat gccgaagcc tacgtccat gtcaaccag 
      tatgatatt gttttggac gttttgata gtcaaccag 
      accaacgat acgttacaa aactgcacc cttgagttg 
      gcaactctc ggcgatttg aaactcgtg gaaaaaccg 
      cagccggtg gttctggcg cccaaagac ttctgcaac 
      attaaggcc aacgtgaaa gtggcctcg accgaaaac 
      gggattatt ttcgggaac atagtttat gacgtcacg 
      ggagcagcg tccgaccga aacgtggtg gtcctggac 
      gacatccat attgatatt atggattac atagtcccg 
      gccacttgt agcgactcg gagttcagg caaatgtgg 
      gccgagttc gagtgggag aataaggtt tcggttaat 
      acgacctta accgacctt gcggaatat ttgaagcac 
      ttgatcaaa agcaccaac atgaagtgt ttgactccg 
      gagaaggcc ctgtcaggg cagtgcggt ttcatggcc 
      gccaatatg tacgcaaaa tcaatcttt ggagaggac 
      gctctcgct aatttgagt attgaaaaa ccgttcaac 
      aaacctgat gcacccgtt actggacat atcaggatc 
      agggccaag agtcagggg atggctctg agtttgggt 
      gacaagatc aatatgaca cagaagggg caacagagt 
      aaagttatc gctgcataa tttgtattt tatggtcgt 
      attaatatt aataaagtt tatttgaaa aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY