General information | |||
TC000620 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 8959442..8964318 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC000620 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00144 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
mature egg not present - Number of animals showing the phenotype: 4
tropharium sometimes degenerated - Number of animals showing the phenotype: 3
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC000620 RA
MEELNDKFY YVYSSSISN RIQIKIGTL EGKRQKPEY DTLLSDPIL KYSGLCEDG CSDLMVVCQ IYDQNQPLA LPVSTSYKA FTSRWSWNE WLTLPVQFN DLPRTAQLA LTIYDCVGP NKLWPVGGT TISLFSKHG LFRQGMVDL RVFPNREAD GNYPTQTPG KAKDQGKEQ MQRLTKLTK KHRNGHMTK VDWLDRLTF RELGLINNE EKSCSEYMY LMIEFPTVY IDLIPHYVV YFEQNGEDI HSFRAQSDI VTVPDQEIL KENLVERKH HKLARSLRS GHCDKDAKP NAAIRDILN TIVSYPSIK PLTNEEQDH VWKFRFYLS TQKKALTKF LKCVNWTQQ NEVRQALSM MRIWAPMDV EDALELLSP NFTHPAVRK YAISRLQQA PDEEILLYL LQLVQALKY EDFKDIQEG YSRISPGRE AIMVHEEKE AQLEKKDSK ESNSTITNE SVMHRSSSY NQADQLASS NVNSVIEVD ADQRAASLP ENILHNLNV YTDSANTDT LQDHMNEEE EVQDLATFL IQRACKNAK LANYFYWYL LIECEDPEP TMKQNMVIR ELYLTVMKT FSQTLAKGN AEMQRKRTN LSLQQKFID NLVKLVKTV LREGGNRKK KAEKLQQLL ADPTVFKFN FGNFEPIPF PLDPDVTIK GIIAEKASL FKSALMPSK LHFITSDNS EYVAIFKHG DDLRQDQLI LQMITLMDK LLRKENLDL KLTPYKVLA TSTKHGFVQ YIDSVSVAE AVNAEGSIH NYFRKYHPQ ENGPYGIAP EIMDSYVRS CAGYCVITY LLGVGDRHL DNLLVTTCG KLFHIDFGF ILGRDPKPL PPPMKLSKE MVEAMGGGN SEHYQEFRK QCYTAFLHL RRHANLMLN LFSLMIDTS VPDIALEPD KAVKKVQEK FRLDLGEEE AVHYIQNLI DASASAVMP AIVEQFHKI TQYIRK
- >TC000620 RA
atggaagaa ctcaacgat aaattctac tacgtgtac agttcctcg atttccaac cgcattcag atcaaaatt gggactttg gaaggcaaa cgccaaaag cccgaatac gacacgctt ctgtccgac cctatttta aaatactcg ggtttgtgt gaggatggt tgttcagac ttgatggtg gtgtgccaa atatacgat caaaaccaa ccgctcgct cttcctgtg tcaacttcc tataaagcg ttcacatcg cgctggagc tggaacgaa tggctcact ttgccagtt cagtttaac gaccttccg agaactgct caattagcc ctaacgatc tacgattgt gtggggccc aacaagcta tggccagtt gggggcacc acgatttcg ctgttcagc aaacatgga cttttcaga cagggcatg gtggatttg agggttttc cccaatagg gaggccgat gggaattac ccaactcaa acgccgggg aaagccaaa gaccaaggg aaggaacaa atgcaaaga ctgacgaaa ttaaccaaa aaacacaga aatggacac atgactaaa gtggattgg ctagatagg ttgactttt agggagtta ggtttgata aataacgag gagaagagt tgttcagaa tacatgtac ttaatgatc gagtttcca actgtttac attgattta attccgcat tatgtcgtg tattttgag caaaatggt gaagatata cattcgttt agggcccag agtgacatt gttacggtc cccgaccaa gaaatccta aaggaaaac ctcgtcgaa cgcaaacac cacaaattg gcgcgttct ttacgcagc ggccactgc gataaagac gccaaaccc aatgctgca atccgcgat atcctcaac accatagtc tcctaccct tccatcaaa ccgctaacg aacgaagag caagaccat gtatggaag ttccgtttt tacctcagc acccagaaa aaagcccta actaagttt ctaaaatgt gtgaattgg acgcaacag aacgaagtg cgacaggcg ctgtctatg atgcggatc tgggccccc atggacgtg gaagacgcc ctcgagctg ctgtcgccg aattttacg cacccagct gtgcgcaaa tacgccatt tccaggctg cagcaagcg cccgacgag gagattctg ctgtatttg ctccagttg gtgcaagcg ctgaagtat gaggatttt aaggatata caagagggg tattcgagg atttctcca ggacgggag gcgataatg gtgcatgag gagaaggag gcgcagttg gagaagaaa gacagtaag gagagcaac agcacgata acaaatgag tcggttatg caccgatca tcgagttat aaccaagcc gatcagctt gcctcttct aacgtcaac agtgtgata gaagtggac gctgatcaa cgcgctgct agtttaccc gaaaatatt ttacacaac ctcaacgtc tacaccgac tcggccaac actgacacg ctacaagac cacatgaac gaagaggaa gaagtccaa gatttggcc acgtttttg atacaaagg gcgtgcaaa aatgccaaa ttggccaat tatttctac tggtatttg ttgatcgag tgtgaggat ccagaaccg accatgaaa caaaacatg gtcataaga gagctgtac ctgacggtg atgaaaacg ttttcgcag actttggcc aaaggcaac gccgagatg caaaggaag cggacaaat ctgtcgctg cagcaaaaa ttcatcgat aatttggtc aagttggta aaaacggtg ttgcgtgag ggcggcaat aggaagaaa aaggccgag aaattgcag cagttgttg gccgatccg actgtgttt aagtttaat tttggtaat ttcgagccg attccgttc ccgcttgat cccgatgtc acaataaag gggataatt gcggaaaaa gcgagtttg ttcaagtcg gctttgatg ccgtccaaa ctgcacttt atcacgagt gataacagc gaatatgtg gccattttc aaacatggg gatgatttg aggcaagac cagttgatt ctgcaaatg ataactctg atggataag ttgttacgg aaggaaaac ctcgatttg aagctaaca ccgtataaa gtactcgcg actagtacc aagcacggt tttgtgcaa tatatcgat tctgtgagt gtggcagag gctgtgaat gcggaaggg agtattcat aactacttt cggaagtac cacccgcag gaaaacggg ccttacggc atcgccccc gaaataatg gacagttac gtgcggtcc tgtgccggt tattgcgtc attacttac ctactgggt gtgggcgat cgtcattta gataattta ttagtgacc acttgtggt aaattattt cacatcgat ttcgggttt atcctaggg cgggaccct aaacccttg cctccgcca atgaaattg agtaaagaa atggtggag gcgatgggg ggaggcaat tctgagcat taccaagag tttaggaag caatgctat actgcgttt ttgcacttg aggaggcat gccaactta atgttgaat cttttttca ttgatgatt gacacgagt gtgccagat attgctcta gagccggat aaggcggtc aaaaaggtg caagagaag tttaggttg gatttaggg gaggaggag gcggtgcat tatatccag aacttgatt gatgcgtcg gcttcggcc gttatgccg gctattgta gaacagttt cataaaatc acccagtat ataaggaag taa
- >TC000620 RA
cattaaaaa taaataaac aaataatcc acgtaaatg agggcttta agccgtggc aatggaaga actcaacga taaattcta ctacgtgta cagttcctc gatttccaa ccgcattca gatcaaaat tgggacttt ggaaggcaa acgccaaaa gcccgaata cgacacgct tctgtccga ccctatttt aaaatactc gggtttgtg tgaggatgg ttgttcaga cttgatggt ggtgtgcca aatatacga tcaaaacca accgctcgc tcttcctgt gtcaacttc ctataaagc gttcacatc gcgctggag ctggaacga atggctcac tttgccagt tcagtttaa cgaccttcc gagaactgc tcaattagc cctaacgat ctacgattg tgtggggcc caacaagct atggccagt tgggggcac cacgatttc gctgttcag caaacatgg acttttcag acagggcat ggtggattt gagggtttt ccccaatag ggaggccga tgggaatta cccaactca aacgccggg gaaagccaa agaccaagg gaaggaaca aatgcaaag actgacgaa attaaccaa aaaacacag aaatggaca catgactaa agtggattg gctagatag gttgacttt tagggagtt aggtttgat aaataacga ggagaagag ttgttcaga atacatgta cttaatgat cgagtttcc aactgttta cattgattt aattccgca ttatgtcgt gtattttga gcaaaatgg tgaagatat acattcgtt tagggccca gagtgacat tgttacggt ccccgacca agaaatcct aaaggaaaa cctcgtcga acgcaaaca ccacaaatt ggcgcgttc tttacgcag cggccactg cgataaaga cgccaaacc caatgctgc aatccgcga tatcctcaa caccatagt ctcctaccc ttccatcaa accgctaac gaacgaaga gcaagacca tgtatggaa gttccgttt ttacctcag cacccagaa aaaagccct aactaagtt tctaaaatg tgtgaattg gacgcaaca gaacgaagt gcgacaggc gctgtctat gatgcggat ctgggcccc catggacgt ggaagacgc cctcgagct gctgtcgcc gaattttac gcacccagc tgtgcgcaa atacgccat ttccaggct gcagcaagc gcccgacga ggagattct gctgtattt gctccagtt ggtgcaagc gctgaagta tgaggattt taaggatat acaagaggg gtattcgag gatttctcc aggacggga ggcgataat ggtgcatga ggagaagga ggcgcagtt ggagaagaa agacagtaa ggagagcaa cagcacgat aacaaatga gtcggttat gcaccgatc atcgagtta taaccaagc cgatcagct tgcctcttc taacgtcaa cagtgtgat agaagtgga cgctgatca acgcgctgc tagtttacc cgaaaatat tttacacaa cctcaacgt ctacaccga ctcggccaa cactgacac gctacaaga ccacatgaa cgaagagga agaagtcca agatttggc cacgttttt gatacaaag ggcgtgcaa aaatgccaa attggccaa ttatttcta ctggtattt gttgatcga gtgtgagga tccagaacc gaccatgaa acaaaacat ggtcataag agagctgta cctgacggt gatgaaaac gttttcgca gactttggc caaaggcaa cgccgagat gcaaaggaa gcggacaaa tctgtcgct gcagcaaaa attcatcga taatttggt caagttggt aaaaacggt gttgcgtga gggcggcaa taggaagaa aaaggccga gaaattgca gcagttgtt ggccgatcc gactgtgtt taagtttaa ttttggtaa tttcgagcc gattccgtt cccgcttga tcccgatgt cacaataaa ggggataat tgcggaaaa agcgagttt gttcaagtc ggctttgat gccgtccaa actgcactt tatcacgag tgataacag cgaatatgt ggccatttt caaacatgg ggatgattt gaggcaaga ccagttgat tctgcaaat gataactct gatggataa gttgttacg gaaggaaaa cctcgattt gaagctaac accgtataa agtactcgc gactagtac caagcacgg ttttgtgca atatatcga ttctgtgag tgtggcaga ggctgtgaa tgcggaagg gagtattca taactactt tcggaagta ccacccgca ggaaaacgg gccttacgg catcgcccc cgaaataat ggacagtta cgtgcggtc ctgtgccgg ttattgcgt cattactta cctactggg tgtgggcga tcgtcattt agataattt attagtgac cacttgtgg taaattatt tcacatcga tttcgggtt tatcctagg gcgggaccc taaaccctt gcctccgcc aatgaaatt gagtaaaga aatggtgga ggcgatggg gggaggcaa ttctgagca ttaccaaga gtttaggaa gcaatgcta tactgcgtt tttgcactt gaggaggca tgccaactt aatgttgaa tcttttttc attgatgat tgacacgag tgtgccaga tattgctct agagccgga taaggcggt caaaaaggt gcaagagaa gtttaggtt ggatttagg ggaggagga ggcggtgca ttatatcca gaacttgat tgatgcgtc ggcttcggc cgttatgcc ggctattgt agaacagtt tcataaaat cacccagta tataaggaa gtaaaacgt gtataaata aggttagtt gtttaataa aatttgtat aaggaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
