Status
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Gene information
General information
TC000620 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8959442..8964318
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000620
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00144

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber differentiation aborted - Number of animals showing the phenotype: 4
    mature egg not present - Number of animals showing the phenotype: 4

    tropharium sometimes degenerated - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection



    Pupal morphology
    abdomen gin-trap setae number decreased - Number of animals showing the phenotype: 9

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000620 RA
      MEELNDKFY YVYSSSISN RIQIKIGTL EGKRQKPEY 
      DTLLSDPIL KYSGLCEDG CSDLMVVCQ IYDQNQPLA 
      LPVSTSYKA FTSRWSWNE WLTLPVQFN DLPRTAQLA 
      LTIYDCVGP NKLWPVGGT TISLFSKHG LFRQGMVDL 
      RVFPNREAD GNYPTQTPG KAKDQGKEQ MQRLTKLTK 
      KHRNGHMTK VDWLDRLTF RELGLINNE EKSCSEYMY 
      LMIEFPTVY IDLIPHYVV YFEQNGEDI HSFRAQSDI 
      VTVPDQEIL KENLVERKH HKLARSLRS GHCDKDAKP 
      NAAIRDILN TIVSYPSIK PLTNEEQDH VWKFRFYLS 
      TQKKALTKF LKCVNWTQQ NEVRQALSM MRIWAPMDV 
      EDALELLSP NFTHPAVRK YAISRLQQA PDEEILLYL 
      LQLVQALKY EDFKDIQEG YSRISPGRE AIMVHEEKE 
      AQLEKKDSK ESNSTITNE SVMHRSSSY NQADQLASS 
      NVNSVIEVD ADQRAASLP ENILHNLNV YTDSANTDT 
      LQDHMNEEE EVQDLATFL IQRACKNAK LANYFYWYL 
      LIECEDPEP TMKQNMVIR ELYLTVMKT FSQTLAKGN 
      AEMQRKRTN LSLQQKFID NLVKLVKTV LREGGNRKK 
      KAEKLQQLL ADPTVFKFN FGNFEPIPF PLDPDVTIK 
      GIIAEKASL FKSALMPSK LHFITSDNS EYVAIFKHG 
      DDLRQDQLI LQMITLMDK LLRKENLDL KLTPYKVLA 
      TSTKHGFVQ YIDSVSVAE AVNAEGSIH NYFRKYHPQ 
      ENGPYGIAP EIMDSYVRS CAGYCVITY LLGVGDRHL 
      DNLLVTTCG KLFHIDFGF ILGRDPKPL PPPMKLSKE 
      MVEAMGGGN SEHYQEFRK QCYTAFLHL RRHANLMLN 
      LFSLMIDTS VPDIALEPD KAVKKVQEK FRLDLGEEE 
      AVHYIQNLI DASASAVMP AIVEQFHKI TQYIRK
    CDS sequence
    • >TC000620 RA
      atggaagaa ctcaacgat aaattctac tacgtgtac 
      agttcctcg atttccaac cgcattcag atcaaaatt 
      gggactttg gaaggcaaa cgccaaaag cccgaatac 
      gacacgctt ctgtccgac cctatttta aaatactcg 
      ggtttgtgt gaggatggt tgttcagac ttgatggtg 
      gtgtgccaa atatacgat caaaaccaa ccgctcgct 
      cttcctgtg tcaacttcc tataaagcg ttcacatcg 
      cgctggagc tggaacgaa tggctcact ttgccagtt 
      cagtttaac gaccttccg agaactgct caattagcc 
      ctaacgatc tacgattgt gtggggccc aacaagcta 
      tggccagtt gggggcacc acgatttcg ctgttcagc 
      aaacatgga cttttcaga cagggcatg gtggatttg 
      agggttttc cccaatagg gaggccgat gggaattac 
      ccaactcaa acgccgggg aaagccaaa gaccaaggg 
      aaggaacaa atgcaaaga ctgacgaaa ttaaccaaa 
      aaacacaga aatggacac atgactaaa gtggattgg 
      ctagatagg ttgactttt agggagtta ggtttgata 
      aataacgag gagaagagt tgttcagaa tacatgtac 
      ttaatgatc gagtttcca actgtttac attgattta 
      attccgcat tatgtcgtg tattttgag caaaatggt 
      gaagatata cattcgttt agggcccag agtgacatt 
      gttacggtc cccgaccaa gaaatccta aaggaaaac 
      ctcgtcgaa cgcaaacac cacaaattg gcgcgttct 
      ttacgcagc ggccactgc gataaagac gccaaaccc 
      aatgctgca atccgcgat atcctcaac accatagtc 
      tcctaccct tccatcaaa ccgctaacg aacgaagag 
      caagaccat gtatggaag ttccgtttt tacctcagc 
      acccagaaa aaagcccta actaagttt ctaaaatgt 
      gtgaattgg acgcaacag aacgaagtg cgacaggcg 
      ctgtctatg atgcggatc tgggccccc atggacgtg 
      gaagacgcc ctcgagctg ctgtcgccg aattttacg 
      cacccagct gtgcgcaaa tacgccatt tccaggctg 
      cagcaagcg cccgacgag gagattctg ctgtatttg 
      ctccagttg gtgcaagcg ctgaagtat gaggatttt 
      aaggatata caagagggg tattcgagg atttctcca 
      ggacgggag gcgataatg gtgcatgag gagaaggag 
      gcgcagttg gagaagaaa gacagtaag gagagcaac 
      agcacgata acaaatgag tcggttatg caccgatca 
      tcgagttat aaccaagcc gatcagctt gcctcttct 
      aacgtcaac agtgtgata gaagtggac gctgatcaa 
      cgcgctgct agtttaccc gaaaatatt ttacacaac 
      ctcaacgtc tacaccgac tcggccaac actgacacg 
      ctacaagac cacatgaac gaagaggaa gaagtccaa 
      gatttggcc acgtttttg atacaaagg gcgtgcaaa 
      aatgccaaa ttggccaat tatttctac tggtatttg 
      ttgatcgag tgtgaggat ccagaaccg accatgaaa 
      caaaacatg gtcataaga gagctgtac ctgacggtg 
      atgaaaacg ttttcgcag actttggcc aaaggcaac 
      gccgagatg caaaggaag cggacaaat ctgtcgctg 
      cagcaaaaa ttcatcgat aatttggtc aagttggta 
      aaaacggtg ttgcgtgag ggcggcaat aggaagaaa 
      aaggccgag aaattgcag cagttgttg gccgatccg 
      actgtgttt aagtttaat tttggtaat ttcgagccg 
      attccgttc ccgcttgat cccgatgtc acaataaag 
      gggataatt gcggaaaaa gcgagtttg ttcaagtcg 
      gctttgatg ccgtccaaa ctgcacttt atcacgagt 
      gataacagc gaatatgtg gccattttc aaacatggg 
      gatgatttg aggcaagac cagttgatt ctgcaaatg 
      ataactctg atggataag ttgttacgg aaggaaaac 
      ctcgatttg aagctaaca ccgtataaa gtactcgcg 
      actagtacc aagcacggt tttgtgcaa tatatcgat 
      tctgtgagt gtggcagag gctgtgaat gcggaaggg 
      agtattcat aactacttt cggaagtac cacccgcag 
      gaaaacggg ccttacggc atcgccccc gaaataatg 
      gacagttac gtgcggtcc tgtgccggt tattgcgtc 
      attacttac ctactgggt gtgggcgat cgtcattta 
      gataattta ttagtgacc acttgtggt aaattattt 
      cacatcgat ttcgggttt atcctaggg cgggaccct 
      aaacccttg cctccgcca atgaaattg agtaaagaa 
      atggtggag gcgatgggg ggaggcaat tctgagcat 
      taccaagag tttaggaag caatgctat actgcgttt 
      ttgcacttg aggaggcat gccaactta atgttgaat 
      cttttttca ttgatgatt gacacgagt gtgccagat 
      attgctcta gagccggat aaggcggtc aaaaaggtg 
      caagagaag tttaggttg gatttaggg gaggaggag 
      gcggtgcat tatatccag aacttgatt gatgcgtcg 
      gcttcggcc gttatgccg gctattgta gaacagttt 
      cataaaatc acccagtat ataaggaag taa
    mRNA sequence
    • >TC000620 RA
      cattaaaaa taaataaac aaataatcc acgtaaatg 
      agggcttta agccgtggc aatggaaga actcaacga 
      taaattcta ctacgtgta cagttcctc gatttccaa 
      ccgcattca gatcaaaat tgggacttt ggaaggcaa 
      acgccaaaa gcccgaata cgacacgct tctgtccga 
      ccctatttt aaaatactc gggtttgtg tgaggatgg 
      ttgttcaga cttgatggt ggtgtgcca aatatacga 
      tcaaaacca accgctcgc tcttcctgt gtcaacttc 
      ctataaagc gttcacatc gcgctggag ctggaacga 
      atggctcac tttgccagt tcagtttaa cgaccttcc 
      gagaactgc tcaattagc cctaacgat ctacgattg 
      tgtggggcc caacaagct atggccagt tgggggcac 
      cacgatttc gctgttcag caaacatgg acttttcag 
      acagggcat ggtggattt gagggtttt ccccaatag 
      ggaggccga tgggaatta cccaactca aacgccggg 
      gaaagccaa agaccaagg gaaggaaca aatgcaaag 
      actgacgaa attaaccaa aaaacacag aaatggaca 
      catgactaa agtggattg gctagatag gttgacttt 
      tagggagtt aggtttgat aaataacga ggagaagag 
      ttgttcaga atacatgta cttaatgat cgagtttcc 
      aactgttta cattgattt aattccgca ttatgtcgt 
      gtattttga gcaaaatgg tgaagatat acattcgtt 
      tagggccca gagtgacat tgttacggt ccccgacca 
      agaaatcct aaaggaaaa cctcgtcga acgcaaaca 
      ccacaaatt ggcgcgttc tttacgcag cggccactg 
      cgataaaga cgccaaacc caatgctgc aatccgcga 
      tatcctcaa caccatagt ctcctaccc ttccatcaa 
      accgctaac gaacgaaga gcaagacca tgtatggaa 
      gttccgttt ttacctcag cacccagaa aaaagccct 
      aactaagtt tctaaaatg tgtgaattg gacgcaaca 
      gaacgaagt gcgacaggc gctgtctat gatgcggat 
      ctgggcccc catggacgt ggaagacgc cctcgagct 
      gctgtcgcc gaattttac gcacccagc tgtgcgcaa 
      atacgccat ttccaggct gcagcaagc gcccgacga 
      ggagattct gctgtattt gctccagtt ggtgcaagc 
      gctgaagta tgaggattt taaggatat acaagaggg 
      gtattcgag gatttctcc aggacggga ggcgataat 
      ggtgcatga ggagaagga ggcgcagtt ggagaagaa 
      agacagtaa ggagagcaa cagcacgat aacaaatga 
      gtcggttat gcaccgatc atcgagtta taaccaagc 
      cgatcagct tgcctcttc taacgtcaa cagtgtgat 
      agaagtgga cgctgatca acgcgctgc tagtttacc 
      cgaaaatat tttacacaa cctcaacgt ctacaccga 
      ctcggccaa cactgacac gctacaaga ccacatgaa 
      cgaagagga agaagtcca agatttggc cacgttttt 
      gatacaaag ggcgtgcaa aaatgccaa attggccaa 
      ttatttcta ctggtattt gttgatcga gtgtgagga 
      tccagaacc gaccatgaa acaaaacat ggtcataag 
      agagctgta cctgacggt gatgaaaac gttttcgca 
      gactttggc caaaggcaa cgccgagat gcaaaggaa 
      gcggacaaa tctgtcgct gcagcaaaa attcatcga 
      taatttggt caagttggt aaaaacggt gttgcgtga 
      gggcggcaa taggaagaa aaaggccga gaaattgca 
      gcagttgtt ggccgatcc gactgtgtt taagtttaa 
      ttttggtaa tttcgagcc gattccgtt cccgcttga 
      tcccgatgt cacaataaa ggggataat tgcggaaaa 
      agcgagttt gttcaagtc ggctttgat gccgtccaa 
      actgcactt tatcacgag tgataacag cgaatatgt 
      ggccatttt caaacatgg ggatgattt gaggcaaga 
      ccagttgat tctgcaaat gataactct gatggataa 
      gttgttacg gaaggaaaa cctcgattt gaagctaac 
      accgtataa agtactcgc gactagtac caagcacgg 
      ttttgtgca atatatcga ttctgtgag tgtggcaga 
      ggctgtgaa tgcggaagg gagtattca taactactt 
      tcggaagta ccacccgca ggaaaacgg gccttacgg 
      catcgcccc cgaaataat ggacagtta cgtgcggtc 
      ctgtgccgg ttattgcgt cattactta cctactggg 
      tgtgggcga tcgtcattt agataattt attagtgac 
      cacttgtgg taaattatt tcacatcga tttcgggtt 
      tatcctagg gcgggaccc taaaccctt gcctccgcc 
      aatgaaatt gagtaaaga aatggtgga ggcgatggg 
      gggaggcaa ttctgagca ttaccaaga gtttaggaa 
      gcaatgcta tactgcgtt tttgcactt gaggaggca 
      tgccaactt aatgttgaa tcttttttc attgatgat 
      tgacacgag tgtgccaga tattgctct agagccgga 
      taaggcggt caaaaaggt gcaagagaa gtttaggtt 
      ggatttagg ggaggagga ggcggtgca ttatatcca 
      gaacttgat tgatgcgtc ggcttcggc cgttatgcc 
      ggctattgt agaacagtt tcataaaat cacccagta 
      tataaggaa gtaaaacgt gtataaata aggttagtt 
      gtttaataa aatttgtat aaggaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY