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Gene information
General information
TC000620 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 3452899..3457775
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000620
    iBeetle screen iB_00144
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Fertility
    vitellogenic egg chamber differentiation aborted - Number of animals showing the phenotype: 4
    mature egg not present - Number of animals showing the phenotype: 4

    tropharium sometimes degenerated - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection


    Pupal morphology
    abdomen gin-trap setae number decreased - Number of animals showing the phenotype: 9

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000620 RA
      MEELNDKFY YVYSSSISN RIQIKIGTL EGKRQKPEY 
DTLLSDPIL KYSGLCEDG CSDLMVVCQ IYDQNQPLA 
LPVSTSYKA FTSRWSWNE WLTLPVQFN DLPRTAQLA 
LTIYDCVGP NKLWPVGGT TISLFSKHG LFRQGMVDL 
RVFPNREAD GNYPTQTPG KAKDQGKEQ MQRLTKLTK 
KHRNGHMTK VDWLDRLTF RELGLINNE EKSCSEYMY 
LMIEFPTVY IDLIPHYVV YFEQNGEDI HSFRAQSDI 
VTVPDQEIL KENLVERKH HKLARSLRS GHCDKDAKP 
NAAIRDILN TIVSYPSIK PLTNEEQDH VWKFRFYLS 
TQKKALTKF LKCVNWTQQ NEVRQALSM MRIWAPMDV 
EDALELLSP NFTHPAVRK YAISRLQQA PDEEILLYL 
LQLVQALKY EDFKDIQEG YSRISPGRE AIMVHEEKE 
AQLEKKDSK ESNSTITNE SVMHRSSSY NQADQLASS 
NVNSVIEVD ADQRAASLP ENILHNLNV YTDSANTDT 
LQDHMNEEE EVQDLATFL IQRACKNAK LANYFYWYL 
LIECEDPEP TMKQNMVIR ELYLTVMKT FSQTLAKGN 
AEMQRKRTN LSLQQKFID NLVKLVKTV LREGGNRKK 
KAEKLQQLL ADPTVFKFN FGNFEPIPF PLDPDVTIK 
GIIAEKASL FKSALMPSK LHFITSDNS EYVAIFKHG 
DDLRQDQLI LQMITLMDK LLRKENLDL KLTPYKVLA 
TSTKHGFVQ YIDSVSVAE AVNAEGSIH NYFRKYHPQ 
ENGPYGIAP EIMDSYVRS CAGYCVITY LLGVGDRHL 
DNLLVTTCG KLFHIDFGF ILGRDPKPL PPPMKLSKE 
MVEAMGGGN SEHYQEFRK QCYTAFLHL RRHANLMLN 
LFSLMIDTS VPDIALEPD KAVKKVQEK FRLDLGEEE 
AVHYIQNLI DASASAVMP AIVEQFHKI TQYIRK
    CDS sequence
    • >TC000620 RA
      atggaagaa ctcaacgat aaattctac tacgtgtac 
agttcctcg atttccaac cgcattcag atcaaaatt 
gggactttg gaaggcaaa cgccaaaag cccgaatac 
gacacgctt ctgtccgac cctatttta aaatactcg 
ggtttgtgt gaggatggt tgttcagac ttgatggtg 
gtgtgccaa atatacgat caaaaccaa ccgctcgct 
cttcctgtg tcaacttcc tataaagcg ttcacatcg 
cgctggagc tggaacgaa tggctcact ttgccagtt 
cagtttaac gaccttccg agaactgct caattagcc 
ctaacgatc tacgattgt gtggggccc aacaagcta 
tggccagtt gggggcacc acgatttcg ctgttcagc 
aaacatgga cttttcaga cagggcatg gtggatttg 
agggttttc cccaatagg gaggccgat gggaattac 
ccaactcaa acgccgggg aaagccaaa gaccaaggg 
aaggaacaa atgcaaaga ctgacgaaa ttaaccaaa 
aaacacaga aatggacac atgactaaa gtggattgg 
ctagatagg ttgactttt agggagtta ggtttgata 
aataacgag gagaagagt tgttcagaa tacatgtac 
ttaatgatc gagtttcca actgtttac attgattta 
attccgcat tatgtcgtg tattttgag caaaatggt 
gaagatata cattcgttt agggcccag agtgacatt 
gttacggtc cccgaccaa gaaatccta aaggaaaac 
ctcgtcgaa cgcaaacac cacaaattg gcgcgttct 
ttacgcagc ggccactgc gataaagac gccaaaccc 
aatgctgca atccgcgat atcctcaac accatagtc 
tcctaccct tccatcaaa ccgctaacg aacgaagag 
caagaccat gtatggaag ttccgtttt tacctcagc 
acccagaaa aaagcccta actaagttt ctaaaatgt 
gtgaattgg acgcaacag aacgaagtg cgacaggcg 
ctgtctatg atgcggatc tgggccccc atggacgtg 
gaagacgcc ctcgagctg ctgtcgccg aattttacg 
cacccagct gtgcgcaaa tacgccatt tccaggctg 
cagcaagcg cccgacgag gagattctg ctgtatttg 
ctccagttg gtgcaagcg ctgaagtat gaggatttt 
aaggatata caagagggg tattcgagg atttctcca 
ggacgggag gcgataatg gtgcatgag gagaaggag 
gcgcagttg gagaagaaa gacagtaag gagagcaac 
agcacgata acaaatgag tcggttatg caccgatca 
tcgagttat aaccaagcc gatcagctt gcctcttct 
aacgtcaac agtgtgata gaagtggac gctgatcaa 
cgcgctgct agtttaccc gaaaatatt ttacacaac 
ctcaacgtc tacaccgac tcggccaac actgacacg 
ctacaagac cacatgaac gaagaggaa gaagtccaa 
gatttggcc acgtttttg atacaaagg gcgtgcaaa 
aatgccaaa ttggccaat tatttctac tggtatttg 
ttgatcgag tgtgaggat ccagaaccg accatgaaa 
caaaacatg gtcataaga gagctgtac ctgacggtg 
atgaaaacg ttttcgcag actttggcc aaaggcaac 
gccgagatg caaaggaag cggacaaat ctgtcgctg 
cagcaaaaa ttcatcgat aatttggtc aagttggta 
aaaacggtg ttgcgtgag ggcggcaat aggaagaaa 
aaggccgag aaattgcag cagttgttg gccgatccg 
actgtgttt aagtttaat tttggtaat ttcgagccg 
attccgttc ccgcttgat cccgatgtc acaataaag 
gggataatt gcggaaaaa gcgagtttg ttcaagtcg 
gctttgatg ccgtccaaa ctgcacttt atcacgagt 
gataacagc gaatatgtg gccattttc aaacatggg 
gatgatttg aggcaagac cagttgatt ctgcaaatg 
ataactctg atggataag ttgttacgg aaggaaaac 
ctcgatttg aagctaaca ccgtataaa gtactcgcg 
actagtacc aagcacggt tttgtgcaa tatatcgat 
tctgtgagt gtggcagag gctgtgaat gcggaaggg 
agtattcat aactacttt cggaagtac cacccgcag 
gaaaacggg ccttacggc atcgccccc gaaataatg 
gacagttac gtgcggtcc tgtgccggt tattgcgtc 
attacttac ctactgggt gtgggcgat cgtcattta 
gataattta ttagtgacc acttgtggt aaattattt 
cacatcgat ttcgggttt atcctaggg cgggaccct 
aaacccttg cctccgcca atgaaattg agtaaagaa 
atggtggag gcgatgggg ggaggcaat tctgagcat 
taccaagag tttaggaag caatgctat actgcgttt 
ttgcacttg aggaggcat gccaactta atgttgaat 
cttttttca ttgatgatt gacacgagt gtgccagat 
attgctcta gagccggat aaggcggtc aaaaaggtg 
caagagaag tttaggttg gatttaggg gaggaggag 
gcggtgcat tatatccag aacttgatt gatgcgtcg 
gcttcggcc gttatgccg gctattgta gaacagttt 
cataaaatc acccagtat ataaggaag taa
    mRNA sequence
    • >TC000620 RA
      cattaaaaa taaataaac aaataatcc acgtaaatg 
agggcttta agccgtggc aatggaaga actcaacga 
taaattcta ctacgtgta cagttcctc gatttccaa 
ccgcattca gatcaaaat tgggacttt ggaaggcaa 
acgccaaaa gcccgaata cgacacgct tctgtccga 
ccctatttt aaaatactc gggtttgtg tgaggatgg 
ttgttcaga cttgatggt ggtgtgcca aatatacga 
tcaaaacca accgctcgc tcttcctgt gtcaacttc 
ctataaagc gttcacatc gcgctggag ctggaacga 
atggctcac tttgccagt tcagtttaa cgaccttcc 
gagaactgc tcaattagc cctaacgat ctacgattg 
tgtggggcc caacaagct atggccagt tgggggcac 
cacgatttc gctgttcag caaacatgg acttttcag 
acagggcat ggtggattt gagggtttt ccccaatag 
ggaggccga tgggaatta cccaactca aacgccggg 
gaaagccaa agaccaagg gaaggaaca aatgcaaag 
actgacgaa attaaccaa aaaacacag aaatggaca 
catgactaa agtggattg gctagatag gttgacttt 
tagggagtt aggtttgat aaataacga ggagaagag 
ttgttcaga atacatgta cttaatgat cgagtttcc 
aactgttta cattgattt aattccgca ttatgtcgt 
gtattttga gcaaaatgg tgaagatat acattcgtt 
tagggccca gagtgacat tgttacggt ccccgacca 
agaaatcct aaaggaaaa cctcgtcga acgcaaaca 
ccacaaatt ggcgcgttc tttacgcag cggccactg 
cgataaaga cgccaaacc caatgctgc aatccgcga 
tatcctcaa caccatagt ctcctaccc ttccatcaa 
accgctaac gaacgaaga gcaagacca tgtatggaa 
gttccgttt ttacctcag cacccagaa aaaagccct 
aactaagtt tctaaaatg tgtgaattg gacgcaaca 
gaacgaagt gcgacaggc gctgtctat gatgcggat 
ctgggcccc catggacgt ggaagacgc cctcgagct 
gctgtcgcc gaattttac gcacccagc tgtgcgcaa 
atacgccat ttccaggct gcagcaagc gcccgacga 
ggagattct gctgtattt gctccagtt ggtgcaagc 
gctgaagta tgaggattt taaggatat acaagaggg 
gtattcgag gatttctcc aggacggga ggcgataat 
ggtgcatga ggagaagga ggcgcagtt ggagaagaa 
agacagtaa ggagagcaa cagcacgat aacaaatga 
gtcggttat gcaccgatc atcgagtta taaccaagc 
cgatcagct tgcctcttc taacgtcaa cagtgtgat 
agaagtgga cgctgatca acgcgctgc tagtttacc 
cgaaaatat tttacacaa cctcaacgt ctacaccga 
ctcggccaa cactgacac gctacaaga ccacatgaa 
cgaagagga agaagtcca agatttggc cacgttttt 
gatacaaag ggcgtgcaa aaatgccaa attggccaa 
ttatttcta ctggtattt gttgatcga gtgtgagga 
tccagaacc gaccatgaa acaaaacat ggtcataag 
agagctgta cctgacggt gatgaaaac gttttcgca 
gactttggc caaaggcaa cgccgagat gcaaaggaa 
gcggacaaa tctgtcgct gcagcaaaa attcatcga 
taatttggt caagttggt aaaaacggt gttgcgtga 
gggcggcaa taggaagaa aaaggccga gaaattgca 
gcagttgtt ggccgatcc gactgtgtt taagtttaa 
ttttggtaa tttcgagcc gattccgtt cccgcttga 
tcccgatgt cacaataaa ggggataat tgcggaaaa 
agcgagttt gttcaagtc ggctttgat gccgtccaa 
actgcactt tatcacgag tgataacag cgaatatgt 
ggccatttt caaacatgg ggatgattt gaggcaaga 
ccagttgat tctgcaaat gataactct gatggataa 
gttgttacg gaaggaaaa cctcgattt gaagctaac 
accgtataa agtactcgc gactagtac caagcacgg 
ttttgtgca atatatcga ttctgtgag tgtggcaga 
ggctgtgaa tgcggaagg gagtattca taactactt 
tcggaagta ccacccgca ggaaaacgg gccttacgg 
catcgcccc cgaaataat ggacagtta cgtgcggtc 
ctgtgccgg ttattgcgt cattactta cctactggg 
tgtgggcga tcgtcattt agataattt attagtgac 
cacttgtgg taaattatt tcacatcga tttcgggtt 
tatcctagg gcgggaccc taaaccctt gcctccgcc 
aatgaaatt gagtaaaga aatggtgga ggcgatggg 
gggaggcaa ttctgagca ttaccaaga gtttaggaa 
gcaatgcta tactgcgtt tttgcactt gaggaggca 
tgccaactt aatgttgaa tcttttttc attgatgat 
tgacacgag tgtgccaga tattgctct agagccgga 
taaggcggt caaaaaggt gcaagagaa gtttaggtt 
ggatttagg ggaggagga ggcggtgca ttatatcca 
gaacttgat tgatgcgtc ggcttcggc cgttatgcc 
ggctattgt agaacagtt tcataaaat cacccagta 
tataaggaa gtaaaacgt gtataaata aggttagtt 
gtttaataa aatttgtat aaggaa