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Gene information
General information
TC000610 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 3574220..3576756
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000610
    iBeetle screen iB_00140
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 0 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000610 RA
      MFRLRTVNL VKKKCRQLS IEAVQNKGK IKVNDQVES 
      PLTDLFGRN HTYLRISLS ERCNLRCQY CMPEEGVKL 
      SPSEKILTS DEIIQISRL FVNEGVTKI RLTGGEPTV 
      RRDLLAIIE NLKKIEGLE TVAMTTNGL TLTRQLVAL 
      QRAGLDIVN ISLDTLKRD KYEFITRRK GWERAMMGI 
      DLALQLGYD PVKINCVVM KGFNDDEII DFVGFTKDR 
      NVDIRFIEY MPFTGNKWD LSKLVSYRD MLSVIKKQY 
      PDLYPLENK PNDTSKAWK VPKFRGQVG FITSMSEHF 
      CGSCNRLRI TADGNLKVC LFGNTEISL RDALRSGCS 
      EDDLIALVN AAVKRKKKQ HAGMLNLSQ MENRPMILI 
      APILARAYS TKLSHVTSE GKATMVDVT PKPDTVRTA 
      KARGIVRVN YKTCDLIRD VTAKKGDVL TVAQLAGIM 
      GAKKTSQLI PLCHNIAIS NVQVNVKLN YEQKHIDIE 
      ASVRSVGKT GVEMEALTA VSVAALTVY DMCKSVSHD 
      IVIGEISLV SKSGGKSDF GEPIVLKTL EESVKDTEI 
      RVGAI
    CDS sequence
    • >TC000610 RA
      atgtttcgc ttacgcaca gtaaaccta gtgaaaaaa 
      aagtgccgt caattgtcc atcgaggcg gttcagaat 
      aagggaaaa atcaaggtc aacgatcag gtagaaagt 
      ccgctcaca gacttattt gggagaaac cacacatac 
      ttgcgaata tcactcagt gagagatgt aatttacga 
      tgtcaatat tgtatgcct gaagaaggg gtcaaacta 
      tccccgagc gaaaaaatc ctaacatct gacgaaatc 
      attcaaata tcacgcctt tttgttaac gaaggagtg 
      actaaaatc agactaacg gggggtgag cccactgta 
      cggagagat ttgctcgca atcatagaa aatttgaaa 
      aaaatcgaa gggcttgaa acagtagcg atgaccaca 
      aacggttta actctgaca cgtcagttg gttgcccta 
      caaagggca ggcttagac atagttaat ataagttta 
      gacacccta aagcgggac aaatacgag tttattacg 
      agacgaaaa gggtgggaa agggccatg atggggatt 
      gacttggcc ctacagttg gggtacgac cctgttaaa 
      attaattgt gtcgtcatg aaagggttc aatgacgac 
      gaaataatc gattttgtc ggttttacc aaagataga 
      aacgtcgat ataagattt atcgagtac atgccgttc 
      acgggaaat aaatgggat ttgagtaaa ttagtgtca 
      tatcgagat atgttatct gtgataaaa aaacaatac 
      cccgatttg taccctctt gaaaacaaa ccaaacgat 
      acgtctaaa gcctggaag gtaccaaaa tttcgagga 
      caagtaggt tttataaca tcgatgagt gagcacttt 
      tgtggctct tgcaaccgc ttaagaatc acagctgac 
      gggaatctt aaagtttgc ctctttgga aacactgaa 
      atttcatta cgagatgca ctacgaagt ggttgcagt 
      gaagacgat ctaatcgct ttagtaaac gcggctgtg 
      aaaaggaaa aaaaagcag catgcaggc atgctgaac 
      ctttctcag atggaaaac cggccgatg atactaatc 
      gctcccatt ttggcccgc gcctactca accaagctc 
      agtcacgtc acctccgaa ggcaaggcc actatggtg 
      gacgtcacc ccgaagcca gatactgtg aggacagcc 
      aaagctcgg ggcattgtc cgggtcaat tacaaaacg 
      tgcgattta attcgggac gtcactgcg aaaaaaggc 
      gacgttttg accgttgca caacttgct ggcattatg 
      ggggcgaaa aaaacgtcc caactcatt cccttgtgc 
      cacaatatt gcgattagc aacgtccaa gtcaacgtc 
      aagctcaat tacgagcag aaacacatc gacattgag 
      gcgagtgtg cggtccgtt ggcaagact ggtgtcgag 
      atggaagct ttgaccgct gtcagtgtg gcggcactg 
      actgtttac gatatgtgc aagtcggtc agtcacgac 
      attgtgatt ggtgaaata tctctcgtg agcaagagt 
      gggggcaag agtgacttc ggcgagcca atcgtattg 
      aagacgctc gaggagtct gtcaaggat acggaaata 
      cgggtggga gcaatctga
    mRNA sequence
    • >TC000610 RA
      tcgttccaa tcacaaagt attttctta ttttaatta 
      ttaggaaac aaattgttt attaatttg attgggttc 
      gtcgaatat ttagataag ttctcgtga tgatgacac 
      agccgaatt cagtaagtg tttcgtcga aatgattta 
      tatattaat taagtgcgt cattttttg tctataaaa 
      ttaagtgaa acctgttaa actgtgtca cttgtattt 
      gtatggaag gagtataaa atgtgttaa caaatacat 
      atatgcaag gtcattcca gtctaattg tgtttactt 
      tcgttacta ccagtcact cgaatgcta tcggagttt 
      atctcaaaa acttaagag aagtgataa tcgtgagca 
      gtgcactgt ttacaaaaa attaaaatg tttcgctta 
      cgcacagta aacctagtg aaaaaaaag tgccgtcaa 
      ttgtccatc gaggcggtt cagaataag ggaaaaatc 
      aaggtcaac gatcaggta gaaagtccg ctcacagac 
      ttatttggg agaaaccac acatacttg cgaatatca 
      ctcagtgag agatgtaat ttacgatgt caatattgt 
      atgcctgaa gaaggggtc aaactatcc ccgagcgaa 
      aaaatccta acatctgac gaaatcatt caaatatca 
      cgccttttt gttaacgaa ggagtgact aaaatcaga 
      ctaacgggg ggtgagccc actgtacgg agagatttg 
      ctcgcaatc atagaaaat ttgaaaaaa atcgaaggg 
      cttgaaaca gtagcgatg accacaaac ggtttaact 
      ctgacacgt cagttggtt gccctacaa agggcaggc 
      ttagacata gttaatata agtttagac accctaaag 
      cgggacaaa tacgagttt attacgaga cgaaaaggg 
      tgggaaagg gccatgatg gggattgac ttggcccta 
      cagttgggg tacgaccct gttaaaatt aattgtgtc 
      gtcatgaaa gggttcaat gacgacgaa ataatcgat 
      tttgtcggt tttaccaaa gatagaaac gtcgatata 
      agatttatc gagtacatg ccgttcacg ggaaataaa 
      tgggatttg agtaaatta gtgtcatat cgagatatg 
      ttatctgtg ataaaaaaa caatacccc gatttgtac 
      cctcttgaa aacaaacca aacgatacg tctaaagcc 
      tggaaggta ccaaaattt cgaggacaa gtaggtttt 
      ataacatcg atgagtgag cacttttgt ggctcttgc 
      aaccgctta agaatcaca gctgacggg aatcttaaa 
      gtttgcctc tttggaaac actgaaatt tcattacga 
      gatgcacta cgaagtggt tgcagtgaa gacgatcta 
      atcgcttta gtaaacgcg gctgtgaaa aggaaaaaa 
      aagcagcat gcaggcatg ctgaacctt tctcagatg 
      gaaaaccgg ccgatgata ctaatcgct cccattttg 
      gcccgcgcc tactcaacc aagctcagt cacgtcacc 
      tccgaaggc aaggccact atggtggac gtcaccccg 
      aagccagat actgtgagg acagccaaa gctcggggc 
      attgtccgg gtcaattac aaaacgtgc gatttaatt 
      cgggacgtc actgcgaaa aaaggcgac gttttgacc 
      gttgcacaa cttgctggc attatgggg gcgaaaaaa 
      acgtcccaa ctcattccc ttgtgccac aatattgcg 
      attagcaac gtccaagtc aacgtcaag ctcaattac 
      gagcagaaa cacatcgac attgaggcg agtgtgcgg 
      tccgttggc aagactggt gtcgagatg gaagctttg 
      accgctgtc agtgtggcg gcactgact gtttacgat 
      atgtgcaag tcggtcagt cacgacatt gtgattggt 
      gaaatatct ctcgtgagc aagagtggg ggcaagagt 
      gacttcggc gagccaatc gtattgaag acgctcgag 
      gagtctgtc aaggatacg gaaatacgg gtgggagca 
      atctgatct atcgtttgt tactgttta ttgttacaa 
      aattgcgcc aaaataaat aattactta cttagggtt 
      ttttctgat gaggtctta gaaaaaggt tatcctcag 
      gcatcaaaa ttctaaaaa aaagaaaaa accgaaaaa 
      att