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Gene information
General information
TC000608 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9095585..9097909
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000608
    iBeetle screen iB_00139

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000608 RA
      MTGPVIAGF TKSECLCEP EEINFAQNV DEDAHITVP 
      EWRLHKVQS PIELKHDET NEYDHFEPL EFHGHWDHG 
      GDATLNNNG FTATLRFQN REMPVLVGG PLHEDQYIF 
      EQLHFHWSD DDHSGCEHI FEGQAFSME AHAVHYNKK 
      YGDFKNAAD RHDGLAVVA FFLKATDDD EHPCFNKMT 
      EAVKKIDKI NSTTSLPSD CLTWIREAA QCKGYYTYQ 
      GSLTTEPYT ESVTWIIYP TPIHVSREQ VSEFRKMKS 
      TPCERHNIV KNVRPIQTP PAHKKLDII YARSHKMSE
    CDS sequence
    • >TC000608 RA
      atgaccgga cctgtgatt gctggcttt acgaaaagt 
      gagtgcttg tgcgagcca gaagaaatc aatttcgcc 
      caaaatgtc gacgaagac gctcacata acagttccc 
      gaatggcgc ctccacaag gtccagtcc cccatcgag 
      ctgaagcac gacgagacc aacgaatac gaccacttc 
      gagccctta gagttccac gggcactgg gaccacggg 
      ggcgacgcc actttgaac aacaacggc ttcacagca 
      actctccgg ttccaaaac cgcgaaatg ccggtcctg 
      gtcgggggc cccttgcac gaggaccag tacatattc 
      gaacaattg catttccat tggagcgac gatgatcac 
      agcggatgt gaacatatt ttcgaagga caagcgttc 
      tcaatggaa gcgcatgcc gttcactac aataaaaag 
      tacggggat tttaaaaac gcggccgac aggcatgac 
      gggctcgcc gttgtggcg ttttttctt aaagccaca 
      gatgacgac gaacatccg tgtttcaac aaaatgaca 
      gaagcggtg aaaaagatc gataaaatc aactcgacg 
      accagtctt cccagcgac tgtttgact tggatcaga 
      gaggcggcc caatgcaag ggctattat acatatcag 
      gggtctctg acaacagag ccatacacc gagagcgtc 
      acttggatt atttatccg acgcccatt cacgtctca 
      cgcgaacaa gtttcagag ttcaggaaa atgaaatcg 
      actccttgt gaaagacac aacattgtt aaaaatgta 
      cgaccgatt cagacgccc ccagcgcat aaaaaactc 
      gacatcatt tacgcaaga tcgcacaaa atgagcgaa 
      taa
    mRNA sequence
    • >TC000608 RA
      gtttagttt acccgtgct taaccattt gatgcacgc 
      gaataaaat tacctaagc taaaatgac cggacctgt 
      gattgctgg ctttacgaa aagtgagtg cttgtgcga 
      gccagaaga aatcaattt cgcccaaaa tgtcgacga 
      agacgctca cataacagt tcccgaatg gcgcctcca 
      caaggtcca gtcccccat cgagctgaa gcacgacga 
      gaccaacga atacgacca cttcgagcc cttagagtt 
      ccacgggca ctgggacca cgggggcga cgccacttt 
      gaacaacaa cggcttcac agcaactct ccggttcca 
      aaaccgcga aatgccggt cctggtcgg gggcccctt 
      gcacgagga ccagtacat attcgaaca attgcattt 
      ccattggag cgacgatga tcacagcgg atgtgaaca 
      tattttcga aggacaagc gttctcaat ggaagcgca 
      tgccgttca ctacaataa aaagtacgg ggattttaa 
      aaacgcggc cgacaggca tgacgggct cgccgttgt 
      ggcgttttt tcttaaagc cacagatga cgacgaaca 
      tccgtgttt caacaaaat gacagaagc ggtgaaaaa 
      gatcgataa aatcaactc gacgaccag tcttcccag 
      cgactgttt gacttggat cagagaggc ggcccaatg 
      caagggcta ttatacata tcaggggtc tctgacaac 
      agagccata caccgagag cgtcacttg gattattta 
      tccgacgcc cattcacgt ctcacgcga acaagtttc 
      agagttcag gaaaatgaa atcgactcc ttgtgaaag 
      acacaacat tgttaaaaa tgtacgacc gattcagac 
      gcccccagc gcataaaaa actcgacat catttacgc 
      aagatcgca caaaatgag cgaataatt tcagtatta 
      ttttattac ataactttt taacattat gtaaccagt 
      ttattacaa ataaattca tagtttcca g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY