General information | |||
TC000605 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 9124761..9134019 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC000605 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00138 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)beetle stock: bad (increased lethality),
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 11.11% (includes death as larva, prepupa, pupa, adult)
- >TC000605 RA
MTFMSKCEK SKVSIGHLG ACTVKPAWP NEDSYAAKT LEGGKLRCP APISRLRVE KIEGGLMVA GVVVAANCQ IILPIFGFL FLLVGCVLT AASYRGPGE DEEPDHYAA RIAFTGNSR ILGPACIVV GVLMLAAGF VLCVLTRRA RRREQTIGF HCPLHGDFY PLSPVTSSR TIAAIEKGG NTCLMCWPR KRGPGAIAV GPPQCPHSQ VSSTRSSLA SSPHSQCPT PLPFLVNSG SVGGLVPVT AQLSPDQPF GSIRSLAAG REVASFPLS RTPTPPPSA FERLGSPNL VQVPDEEKL VESNFDRVS RSGPRKSVS IVLPSEDKG
- >TC000605 RA
atgacgttt atgtccaag tgtgaaaaa agcaaagtt tcgatcggc catctggga gcttgtaca gttaagcca gcatggcct aacgaagac tcatacgcg gccaagacg ctggagggc ggcaagttg cggtgcccg gcgccaatt tcccgcctc cgggtggag aaaatcgag gggggtctt atggtggcc ggagtcgtc gtggctgcc aattgccag attatttta cccattttt ggattctta ttcctgctg gttggctgt gttttgacc gctgcgtct tatcgtggt ccaggtgag gacgaagag ccggaccat tatgcagcc cgtatcgca tttacgggt aattcccgc atcttagga ccagcttgt atcgtcgtg ggtgtgctt atgttagcc gctggtttc gttttatgt gttttgact cgaagagcc cgaagacgg gaacaaacc atcggcttt cactgcccc ctccatgga gatttctac cccctaagt cccgtaact agctcaaga acaatagcc gctattgaa aaaggtggc aatacgtgt ttaatgtgt tggcctcga aaaagaggt ccaggtgct atcgctgtg ggccctcct cagtgcccc cacagtcag gtctcgagc acgaggagt tctctggcg agttcgccc catagtcag tgccccacg ccgttacct ttcctcgtt aattcagga tcagtcggt ggcctagtc ccggtgacg gcacagtta tcgccagat caacccttt gggtcgatt cgatcgttg gcagccggc cgagaagtg gctagtttc cctttatcg cggacgccc acgccgccc ccttcagcg tttgagaga ttaggaagt ccgaatcta gtccaagtc cccgacgaa gagaagctc gtagagtca aatttcgac cgagtgtcc cggagcggt cctcgaaaa tcggtctcg attgtattg ccgtcggag gacaaaggg tga
- >TC000605 RA
ccagttccg atgtaatca taattcagt tcgaagttg acgacagtt cggccatgg caattttca caagatgca ccagaaaca tgaccgttg ctaaattgg ggattttgt cggccgtta ctgacatga accaagttc tttgtgtgt ttatcatcg cacggactc tgcgacaag ctttcagaa atttctgaa gcgctcgaa gcgcgtagc gagtttcct gcggagtca gtcgttcgc gtgcagccg gtcagaagc ctcgctctc acactatgg atcatatct ctttgttac ggtaatgaa tgacgatga ttgcccttt gtccgtcac tcgccacca ttcagtgat ctgtgtcct gtcgtgtta aactatgac gtttatgtc caagtgtga aaaaagcaa agtttcgat cggccatct gggagcttg tacagttaa gccagcatg gcctaacga agactcata cgcggccaa gacgctgga gggcggcaa gttgcggtg cccggcgcc aatttcccg cctccgggt ggagaaaat cgagggggg tcttatggt ggccggagt cgtcgtggc tgccaattg ccagattat tttacccat ttttggatt cttattcct gctggttgg ctgtgtttt gaccgctgc gtcttatcg tggtccagg tgaggacga agagccgga ccattatgc agcccgtat cgcatttac gggtaattc ccgcatctt aggaccagc ttgtatcgt cgtgggtgt gcttatgtt agccgctgg tttcgtttt atgtgtttt gactcgaag agcccgaag acgggaaca aaccatcgg ctttcactg ccccctcca tggagattt ctaccccct aagtcccgt aactagctc aagaacaat agccgctat tgaaaaagg tggcaatac gtgtttaat gtgttggcc tcgaaaaag aggtccagg tgctatcgc tgtgggccc tcctcagtg cccccacag tcaggtctc gagcacgag gagttctct ggcgagttc gccccatag tcagtgccc cacgccgtt acctttcct cgttaattc aggatcagt cggtggcct agtcccggt gacggcaca gttatcgcc agatcaacc ctttgggtc gattcgatc gttggcagc cggccgaga agtggctag tttcccttt atcgcggac gcccacgcc gcccccttc agcgtttga gagattagg aagtccgaa tctagtcca agtccccga cgaagagaa gctcgtaga gtcaaattt cgaccgagt gtcccggag cggtcctcg aaaatcggt ctcgattgt attgccgtc ggaggacaa agggtgacc agctgatta agcaatcag cgttcgcgg aaccaagtg tttttcaca aaatccaga ctataaaga cattgtcat cttttttat cgaatttcg ttgtgtgtt tagcatgtt tggtgtcat aaatggcat tgtttagac tgacgtgtt ttattaaaa cctttgtca atgttggac gttccacga gtttggctt tttgcctat aaacgtgct ccgtaagcg agctaaatt ctagtctgg acaagacta aatcaatcg cattgacaa tgggttcaa ttctgtgat tcagatcat gttatacgt aattttttt gcagtagct tattgtttc acatgaaat ttgatatgt gggacatca tcacaatat catgtgtac caaaacggc tgttccccc atagttgct tctgtagtt tttctctga atcacgata ttatgtgag tgatattac ataatttat tttccaatt tgtgtacaa aactgacga aatacttat tcctttctt accacagta tgagaaagt tcgtagtaa atgcacatt taagactga tttatttaa aactacttt atttttaca ttgttaaca tatcaaccg gaaggaata aatatttca cgcacccag tccgtactt agcttgcaa tgggatgac tagaatttt ttttatccg tccttgagt gccaaaatt taacaaaca accaattcg aagcgtttt catttcatt tgattcgtt catctcatt gcaattttt ttgtacata tgttatgcc cgattttta ttcatttag ctttctgtt gagcaaaag ctaaataat aaaaattca actattttg tagtacgac aagtgatat gtattatgt ataattaga ttatatgtc gaggctgat tacacttta agatttagt tttaggata aaatgtttt tactcaacg tgtatatta tacaattta attgttaac aaagtaatg gctctattt cacttgctc attctttta agataagtt gtaaaacag tgattacag ctaactgat ctttattct atacataag tttaatgta ttgatcaat aaatagatt aaacgtaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
