Status
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Gene information
General information
TC000592 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9303869..9306526
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000592
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00134

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 33.33% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 33.33% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000592 RA
      MKPRKTKAK KSDLDESQH HAIKEKPYI TKEIETSIT 
      LLESPESGL VLEALLFLS KYADIQRNN LIFLHSRGL 
      MPKLLALLD HNICIVRLS LRLLSQLLA LEAAQLELN 
      QQKFDERVL KIAGFYTGH KDPHVKEFA ILILAAFAK 
      LGRAHILLA EAGLISAVL KTVKTPESD TILNSSLEL 
      FSKFIEMEQ VRGDLPTEK DFHLDLFIQ HLNTSEACF 
      RTILVIFEK ITYFSDDHL LNRLKECQI VEHMFDIIM 
      DPSHAEHTQ TCLNIVWHC MGNEITRTY FVTTLEFLK 
      FCQWVKNCP PEVGLICAR IFLELAKID SIKQLLFDL 
      SIEDTILSF FKSDDKQVL NVTCDSVCF MSKHKYCCE 
      KIVTPIVVK KLLTILGRQ NDVYDPDNE TALSTLYYL 
      SRRDSRTVH MVRAFDGVE ILMRYLRKK LPQDSYRKL 
      LEIVNMFVT NNQLQKVIL SPDLYEIIL TQTLSKFVE 
      ICKRSLEIM INCLGVEEF RNYFLLNHG SRVIVDKLA 
      TSTDEETIR LLVIFVHNA LIFEKLAND FVLRKILLV 
      LKKIPEGVK YRNPLIQRV INLVFNMCL PVKFFETGR 
      LELMDKLHE RFYVITGPW SEPFPFLEV LEIRRMSTI 
      NTIYVVDDC GHHKTDTKE AHDCFSIQR QSITESALC 
      SSSIISISS DQMKCAINY GRLSHDPFL PKYFSRVEK 
      LTSGVSRLP EKIQILAKF VADCLCGPT EKGSISDKL 
      HTFKLHIEC IKEKLGTSM IPIGYLRLG FHCERALLF 
      KALADHVYI PATLVKGKS KLYWNEVAL IQSEKKAKV 
      LRMFVVDLM DNVGQLLPV GSRECNVYC DIL
    CDS sequence
    • >TC000592 RA
      atgaagccg agaaaaacc aaggcgaaa aagtccgac 
      ttggacgaa tctcagcac cacgccatc aaagaaaag 
      ccgtacata acaaaggaa attgaaaca tcaataacc 
      ctcctcgaa agtccagaa agcggcctc gtattggag 
      gcgctccta ttcctatca aaatatgcg gacattcaa 
      cggaacaac ttaatattc ctgcactcg cgtggcctc 
      atgccaaag ctgctggca ctgctcgac cataacatt 
      tgcatcgtg cgactctcc ttaagacta ttatcccaa 
      ttactggcc ctggaggcc gcccagctc gagctcaac 
      cagcagaag ttcgacgag cgggtgctc aaaatcgcc 
      ggcttttac accggacac aaggacccg cacgtgaaa 
      gagttcgca attctcatc ctggcggcg tttgccaaa 
      ttggggcgc gcccatatt ttactggct gaagccggc 
      ttaatttca gccgttctg aaaacggtg aagacgcct 
      gaaagtgac actattctg aatagttcg cttgaatta 
      ttcagcaag tttatcgaa atggagcaa gtgaggggg 
      gatttaccc acagaaaag gacttccat ttggacttg 
      ttcatccag cacttgaac acttcggaa gcctgcttc 
      aggaccatc ctcgtcatt ttcgaaaaa atcacctac 
      ttcagcgac gaccatctc ctgaaccgg ctcaaagaa 
      tgccaaatt gtcgagcac atgtttgac attatcatg 
      gaccccagc cacgcagag cacacgcag acttgtctc 
      aacatcgtc tggcactgc atggggaac gaaatcacc 
      cggacttac tttgttacg actctcgag tttttgaaa 
      ttctgtcaa tgggtcaaa aactgcccc ccggaggtc 
      ggcctcatc tgtgctaga attttcctc gaactcgcc 
      aaaatcgac tcgattaaa caactcctc tttgactta 
      tcaatcgag gacacgatt ttgtcgttt ttcaaaagt 
      gatgataaa caagtgttg aacgtaacg tgcgattcg 
      gtttgtttc atgtccaag cacaagtac tgttgcgag 
      aagatagtg acgcctata gttgttaaa aaactgctt 
      acgatccta ggccggcaa aacgatgtt tacgaccct 
      gataatgaa acggcttta tcgactttg tattacttg 
      agccggcgc gactctcga acggttcat atggtgcgg 
      gcttttgac ggggttgaa attttgatg aggtatttg 
      cgcaaaaaa ctgccccaa gactcgtac cgcaagctc 
      ttggaaatt gtcaatatg ttcgttact aataatcaa 
      ttgcaaaaa gtgattctt tccccggat ttgtacgag 
      ataattctc acacaaact ttgagtaaa tttgttgaa 
      atatgcaag aggagtttg gaaattatg ataaactgt 
      ttaggcgtc gaagaattt cggaattac tttttatta 
      aatcacggt tcgcgcgtt attgttgat aaactagcg 
      acgagcacg gacgaggaa actatccga ctgcttgtg 
      attttcgtt cacaatgct ttgatcttc gagaaatta 
      gcgaatgat ttcgtgttg aggaaaatc ctgcttgtg 
      cttaagaaa atcccggag ggtgtgaaa taccggaac 
      ccgctgatc caacgcgtt ataaatttg gtgtttaat 
      atgtgtttg ccggtgaag ttttttgag actgggcga 
      ttggaacta atggataag ttgcacgag aggttttac 
      gttatcaca ggtccttgg agcgagccg tttccattt 
      ttggaggtg ttggaaatc aggcgaatg tccaccata 
      aacacaatt tacgtggtc gatgattgt ggacaccac 
      aaaacggac acgaaagaa gcccacgac tgtttcagt 
      atccaaagg cagagcatc acagaatct gctctgtgt 
      tcgtcgtca attatatca atctcctcc gatcaaatg 
      aagtgcgcg ataaattac ggccggctt tcgcacgac 
      ccctttttg cctaaatac ttctcccga gtcgagaaa 
      ctaacaagt ggtgtgtcg cgtttgcct gaaaaaatc 
      caaattctg gcgaaattc gttgcagat tgtctgtgt 
      ggtccgaca gagaagggt tcgatttcg gataagttg 
      cacaccttc aagcttcat atcgaatgc attaaggaa 
      aagctcggt actagcatg atcccgatc gggtatttg 
      cgtttgggg tttcactgt gaaagggct ttgcttttt 
      aaagcattg gctgatcat gtttacata ccggcgact 
      ttggttaaa ggcaagagt aaattgtat tggaacgaa 
      gtagcgctg attcagagc gaaaagaag gcgaaggtt 
      ttgagaatg ttcgtggtg gatttgatg gacaatgtg 
      ggacagctg ttgccggtt ggctcgagg gagtgcaat 
      gtgtattgt gatattctt taa
    mRNA sequence
    • >TC000592 RA
      gtgggaaag gttgcaggc tctcattcc agaaatgga 
      cctaaataa taattaata agcgctcga ctcgcaaaa 
      actccatca gaatttttc acaaattga caaatgatt 
      cattctgac aatcttgtc atttattca gaatgaagc 
      cgagaaaaa ccaaggcga aaaagtccg acttggacg 
      aatctcagc accacgcca tcaaagaaa agccgtaca 
      taacaaagg aaattgaaa catcaataa ccctcctcg 
      aaagtccag aaagcggcc tcgtattgg aggcgctcc 
      tattcctat caaaatatg cggacattc aacggaaca 
      acttaatat tcctgcact cgcgtggcc tcatgccaa 
      agctgctgg cactgctcg accataaca tttgcatcg 
      tgcgactct ccttaagac tattatccc aattactgg 
      ccctggagg ccgcccagc tcgagctca accagcaga 
      agttcgacg agcgggtgc tcaaaatcg ccggctttt 
      acaccggac acaaggacc cgcacgtga aagagttcg 
      caattctca tcctggcgg cgtttgcca aattggggc 
      gcgcccata ttttactgg ctgaagccg gcttaattt 
      cagccgttc tgaaaacgg tgaagacgc ctgaaagtg 
      acactattc tgaatagtt cgcttgaat tattcagca 
      agtttatcg aaatggagc aagtgaggg gggatttac 
      ccacagaaa aggacttcc atttggact tgttcatcc 
      agcacttga acacttcgg aagcctgct tcaggacca 
      tcctcgtca ttttcgaaa aaatcacct acttcagcg 
      acgaccatc tcctgaacc ggctcaaag aatgccaaa 
      ttgtcgagc acatgtttg acattatca tggacccca 
      gccacgcag agcacacgc agacttgtc tcaacatcg 
      tctggcact gcatgggga acgaaatca cccggactt 
      actttgtta cgactctcg agtttttga aattctgtc 
      aatgggtca aaaactgcc ccccggagg tcggcctca 
      tctgtgcta gaattttcc tcgaactcg ccaaaatcg 
      actcgatta aacaactcc tctttgact tatcaatcg 
      aggacacga ttttgtcgt ttttcaaaa gtgatgata 
      aacaagtgt tgaacgtaa cgtgcgatt cggtttgtt 
      tcatgtcca agcacaagt actgttgcg agaagatag 
      tgacgccta tagttgtta aaaaactgc ttacgatcc 
      taggccggc aaaacgatg tttacgacc ctgataatg 
      aaacggctt tatcgactt tgtattact tgagccggc 
      gcgactctc gaacggttc atatggtgc gggcttttg 
      acggggttg aaattttga tgaggtatt tgcgcaaaa 
      aactgcccc aagactcgt accgcaagc tcttggaaa 
      ttgtcaata tgttcgtta ctaataatc aattgcaaa 
      aagtgattc tttccccgg atttgtacg agataattc 
      tcacacaaa ctttgagta aatttgttg aaatatgca 
      agaggagtt tggaaatta tgataaact gtttaggcg 
      tcgaagaat ttcggaatt actttttat taaatcacg 
      gttcgcgcg ttattgttg ataaactag cgacgagca 
      cggacgagg aaactatcc gactgcttg tgattttcg 
      ttcacaatg ctttgatct tcgagaaat tagcgaatg 
      atttcgtgt tgaggaaaa tcctgcttg tgcttaaga 
      aaatcccgg agggtgtga aataccgga acccgctga 
      tccaacgcg ttataaatt tggtgttta atatgtgtt 
      tgccggtga agttttttg agactgggc gattggaac 
      taatggata agttgcacg agaggtttt acgttatca 
      caggtcctt ggagcgagc cgtttccat ttttggagg 
      tgttggaaa tcaggcgaa tgtccacca taaacacaa 
      tttacgtgg tcgatgatt gtggacacc acaaaacgg 
      acacgaaag aagcccacg actgtttca gtatccaaa 
      ggcagagca tcacagaat ctgctctgt gttcgtcgt 
      caattatat caatctcct ccgatcaaa tgaagtgcg 
      cgataaatt acggccggc tttcgcacg acccctttt 
      tgcctaaat acttctccc gagtcgaga aactaacaa 
      gtggtgtgt cgcgtttgc ctgaaaaaa tccaaattc 
      tggcgaaat tcgttgcag attgtctgt gtggtccga 
      cagagaagg gttcgattt cggataagt tgcacacct 
      tcaagcttc atatcgaat gcattaagg aaaagctcg 
      gtactagca tgatcccga tcgggtatt tgcgtttgg 
      ggtttcact gtgaaaggg ctttgcttt ttaaagcat 
      tggctgatc atgtttaca taccggcga ctttggtta 
      aaggcaaga gtaaattgt attggaacg aagtagcgc 
      tgattcaga gcgaaaaga aggcgaagg ttttgagaa 
      tgttcgtgg tggatttga tggacaatg tgggacagc 
      tgttgccgg ttggctcga gggagtgca atgtgtatt 
      gtgatattc tttaataaa tgtccattg ttggaaaaa 
      ttttatgtt atgcctttt ttttaagat ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY