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Gene information
General information
TC000591 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9310555..9340546
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000591
    iBeetle screen iB_00133

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    posterior terminus irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen segment number # of remaining segments increased - Penetrance (percentage of animals/eggs on slide): more than 80%

    urogomphi not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    urogomphi potentially transformed - Penetrance (percentage of animals/eggs on slide): more than 80%
    pygopodium potentially size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupa lethality during eclosion - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection



    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 9

    wings shortened - Number of animals showing the phenotype: 9

    oblique dorsal thoracic muscles partially shortened - Number of animals showing the phenotype: 9

    female genital lobes potentially duplicated - Number of animals showing the phenotype: 9

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000591 RA
      MAGKPELSP APGVQNVVV YVSNDNFAQ AYAVGGQNA 
      PQYGPPKPY PEKRPDYPE GYFVSSNGY GYEERPAKE 
      CDYDPAYVT VMGAEGPPQ SVRCDSVRS ETAESSCSS 
      LSSADEGMV LVQNNQPEM VVYDPSVSV RPGVVFVGA 
      GVHHHPNVA ATAAANLPA AQQPSITVP YGWKRILNN 
      GSIMYISPS NTALSSPEQ VKEYLLTSG TCKCGLECH 
      FKYDNTFNF DPKVASKPW TLSPDTNTG DLTKLCNHK 
      RKIIAMASL ECKPPDPAA KMRKDCNNK KTKRKVGMP 
      YAGCVSLSQ IFTRPDKLA LSQQHFNKE GLGNQAQVW 
      PQSPTSSSQ MGNRLQNYP ESQQMVRYQ NQEQQNRIV 
      NGSPMVVGD NSTMMVPQQ QSNISQQIH QNHNGQNPS 
      VPQQHIIGP NGQVISLQG ALNSNQVNN GVVIQGQNV 
      VPNPNQSRV FINSQPSEP SGPGRPNIQ MIPVGVTNQ 
      RDAQNRLHH FYNNQQYPQ IQYDASKQM RPTFMPGHN 
      QMNGFRPQN NQIMQAPVM INNHQKMPW PNRIQNSPN 
      AYERVPPLH PHTPSPTMW QDEVKRKKV KLGKIVKNR 
      PYHMMDSAL HAPCPNIDV RQIPAENGR QVIINQNSA 
      SPSFMEDPS GYLAQQTAL LNNTINRQT GVNACAGMV 
      CSSPQQPHL TPPNSQTNE VPLPSNVVI SHMKQTQNI 
      TNRTNQTLH VVKSQVPQV MSQQYNQMM AAHADSSVT 
      NDQCQNCDS HKGHSRPNS QPGTPSSSA IDDPVTSST 
      YSDKQNNQS PDSRPIQGG TVSTSNVSP LDGSQSDPP 
      TPNPHTPTP PRNDNMPYA VYQSREVCS TNYPPKSDC 
      CSKMNTPYI SSIVTTMAS GRTVGSNTI TSVLAGRAN 
      TATVSVNSP ANLPASTTP TTVTQTVSK SPLEMVQSV 
      VSSIQVPQS QTNPQISPP HIVKHSPTG LPPGHILVS 
      SGGQLIMAN TGNSQSGVM PPPPPKLVS NQTAMPPMS 
      VSPMITNVT AAVSQVIPA VAQQVLGQQ TVLVNALPT 
      PFVLQGGVT MTMDGSMVG QNMQLPQIV AGNVIQQQI 
      QLDNSDPRR TTALLSPET KKKGKKRKM SQTVANMLH 
      IAAQQNSGV VMSQQGFPQ QIQMAHSPQ GLATGPVMQ 
      ALTIVPSKT GGPPQIVMN GQPMTNTSP QLIANSQPA 
      QQINLLQPV NLINGTTGV VQNFPTIQQ FIVPNLGGM 
      VMNPDGTAT ILQDTSNIG MQLQLQNVN GQNVLTPVQ 
      NNGMFNGGQ SILAASPAG MVIRAPTSQ GKLIQQQQH 
      SPGAQFLSP NGGQFVVNG TQFSGQLSP LVASVSPSQ 
      QVTFGTSTP QIRPTQGQQ EFIQCGQMG QTLMVPCAP 
      TVAVSSSQN TTFVQQNTT IVQQQTTMV SNSQQLQNF 
      QNNGGNRTT VNVDQNFII ANDNKQVQA LVQVQRESP 
      GTNYRQSVS TQTAVNQNA QTVTTFCQT STLCAGSPP 
      DTTTHSPLA SGGQSPATA DTTTHTGST DDGLSPAPS 
      NCSVTCDSA GRQPACTMA MVHCISSSE PDSADLNTQ 
      GSDHDWSRA SSIPQQKSD LSEIHFPKK QNTPTHVAY 
      AESSTMMAG LHFIGDQMK AQEAVVSSH NFDKIVQKR 
      KSSDTLLVM ETHHMHSSL FDEEEDNEK REKSEHDFV 
      VGDLVWGPA KASPAWPGK IIQVDGNDV SVKWFGSEK 
      LITKLDRGS LQTLTEGLD AHHQARKKC RTSRKLNSH 
      LEKAIQEAM AELDKDLEQ ETKEVKQQK KKAHRVRSG 
      HR
    CDS sequence
    • >TC000591 RA
      atggcagga aagcctgag ctgtcgccc gcccccggc 
      gtccagaat gtggtagtg tacgtttcg aacgacaac 
      ttcgcccaa gcgtacgcc gtcgggggg cagaatgcg 
      ccccagtac ggccccccg aagccctac cccgagaag 
      cggcccgac taccccgag ggctacttc gtctcgtcc 
      aacggctac ggatacgag gagcgcccg gccaaggag 
      tgcgactac gacccggcc tatgtgacg gtcatgggg 
      gccgagggg cccccgcag agtgtgcgg tgtgattca 
      gtgcggagc gagacggcc gagtcgagc tgcagcagc 
      ttgagctcg gccgacgag gggatggtg ctagtacag 
      aacaatcaa ccagagatg gtggtgtat gatcccagt 
      gttagtgtt agacccggt gtcgtgttt gtgggggcc 
      ggcgtccac caccacccc aatgtcgcc gccaccgcc 
      gccgccaac ctacccgcc gcccaacag ccctccatc 
      actgtgccc tacggctgg aagaggatt ctcaacaac 
      ggatccatc atgtacata agcccaagt aacacagct 
      ttaagttct ccggaacaa gtcaaagaa tatctcctc 
      acgtcgggc acttgcaag tgtggatta gaatgtcat 
      ttcaaatat gacaacact tttaatttc gatcctaag 
      gtggccagt aaaccgtgg acgctatca ccggacacc 
      aatacaggg gacctgacc aaattgtgc aaccacaag 
      agaaaaatc atcgccatg gcctcgttg gagtgcaag 
      cccccggac cccgccgcc aaaatgcga aaagactgc 
      aacaataag aaaaccaag cgaaaagtc ggcatgcct 
      tacgccggt tgtgtttct ttatcgcaa attttcact 
      cgtccggac aaactcgct ttgagtcaa cagcatttc 
      aacaaggaa ggtctcgga aatcaagct caggtgtgg 
      cctcagtca ccgactagc agctctcaa atgggtaat 
      cgtctgcag aattacccc gagagccag cagatggtc 
      cgatatcaa aaccaggag caacagaat cgaatcgtt 
      aacggttcg cccatggtg gtcggtgat aacagtacc 
      atgatggta ccccaacaa cagtccaac atatcgcaa 
      cagatccac cagaaccac aatggtcaa aatcccagc 
      gttcctcag cagcacatt atcgggccc aacggccaa 
      gtgataagt ctccaaggg gcccttaac tcgaatcag 
      gtcaataat ggagtggtc atacagggt caaaacgtc 
      gttcctaat ccaaatcaa tcccgagtt ttcatcaac 
      agccaacca agcgaaccc agtggacca ggccggccc 
      aacatccag atgattccc gtaggagtc acgaatcaa 
      agagatgcc caaaaccgc ctccaccac ttctacaac 
      aaccaacaa tacccacag atacaatac gacgcgtcg 
      aagcaaatg cgaccgaca tttatgccc ggccacaac 
      caaatgaac ggttttcgc ccgcaaaat aatcagatt 
      atgcaagcg ccggttatg attaacaac catcaaaag 
      atgccatgg cctaatcgc attcaaaat tcgcccaat 
      gcctacgaa agagtgccg ccgctgcat ccacacaca 
      ccatcgccc actatgtgg caagacgag gtcaaacgg 
      aaaaaggtc aaattgggg aaaatcgtt aaaaatcgg 
      ccgtatcat atgatggat tcggcgttg catgcaccc 
      tgtcccaac attgacgtg cggcaaata ccagccgaa 
      aacggccga caggttatt atcaaccag aattcggcc 
      agtccctcg tttatggag gaccccagt gggtattta 
      gcccagcag acggccctg ctcaataat accatcaac 
      agacaaaca ggggtgaac gcgtgcgcg ggcatggtg 
      tgcagcagc ccgcagcag ccccaccta acaccaccg 
      aattcgcaa acaaacgag gtgccttta cccagcaac 
      gtggtgata tctcatatg aaacaaacg caaaacatt 
      acaaatcga accaatcaa acgttacac gtagtaaaa 
      agtcaagtt cctcaagta atgtcccag cagtataat 
      caaatgatg gcggctcac gccgacagt agcgtcacc 
      aacgaccaa tgtcaaaat tgcgacagt cataaaggc 
      cattcgaga cctaacagt caacctgga actccgagt 
      tcgtcggct attgacgat ccggtgaca tcatcgact 
      tattccgac aaacaaaat aatcaatca ccggattca 
      agaccgatt cagggcggt actgttagt actagcaat 
      gtatcgcca ttggatgga tcacaatca gatccacct 
      acacctaac ccacacact ccaacgcct cctaggaat 
      gataatatg ccatatgct gtctaccaa tcgcgtgag 
      gtttgcagt acgaattac ccaccgaaa agtgactgt 
      tgttcgaaa atgaacacg ccgtatatt tcgagtata 
      gttacgacg atggcaagt ggacgaaca gttggtagt 
      aataccata acgtcggtt ttggccggt cgtgccaac 
      actgccaca gtttcagtc aacagtcct gctaacctc 
      ccagcatcc acaacccca acaacagtc acacaaacc 
      gtttcaaaa tccccactt gaaatggtc caaagcgtc 
      gtcagtagc atacaagtc cctcagtct caaaccaat 
      ccccaaatc tctccacca catatcgtc aaacattcc 
      cccaccggg ttacctcct ggccatatc cttgtctca 
      agcggcggc cagttaata atggcaaac accggaaac 
      agccaatcg ggcgttatg ccaccccca ccccccaaa 
      ctagtctcc aatcaaacc gcaatgccg ccaatgtcc 
      gtctcacca atgatcaca aatgtcact gcagcggtc 
      agtcaggtc ataccggca gtagctcag caagtttta 
      ggccaacaa accgtttta gtgaacgcc ttacccaca 
      ccttttgtc ttacaaggc ggagtcaca atgaccatg 
      gacgggtcc atggtcggc caaaacatg caattacct 
      caaatcgtc gccggaaat gtgatacaa caacaaatc 
      caactggat aacagtgac ccacgacgt accaccgcg 
      ctactctca cccgaaacc aagaaaaaa ggaaagaaa 
      cgaaaaatg tcacaaaca gtggccaac atgcttcac 
      atagctgcc cagcaaaac tccggcgtt gtgatgtca 
      caacaaggc ttcccgcag caaattcaa atggctcat 
      agtccgcaa ggtctagcc acgggccca gtgatgcaa 
      gccttgacc atagtcccc agcaaaaca gggggaccc 
      ccacagatc gtaatgaac gggcaaccc atgaccaac 
      accagtccc caactcatt gcaaactcg caaccggcg 
      caacaaatc aacctcctt cagccggtg aatctcatc 
      aatggcacc accggagtg gtgcaaaat ttccccacg 
      attcaacaa ttcatagtc ccgaattta ggcggcatg 
      gttatgaac ccggacggc accgccact atattacag 
      gacacttca aatatcggc atgcagtta caactacag 
      aacgttaac ggacaaaac gtcctgact ccggtccaa 
      aacaacgga atgttcaac gggggtcag agtattcta 
      gcagcgagt ccggccggc atggtcatt agggccccc 
      acctcgcag gggaaatta atccagcag cagcagcac 
      agtccaggt gcccagttt ttgtcaccc aacgggggc 
      cagtttgtc gtcaatggg acacaattc agcggccag 
      ttgagcccg cttgtggct agtgtgagt ccgagtcag 
      caagttacc tttgggacg tccacgccg cagattaga 
      ccaactcag ggtcaacaa gaatttata cagtgtggt 
      caaatgggc cagactttg atggttccg tgtgcacca 
      acggtcgcc gtttcctcc tcgcaaaac acgactttt 
      gtccagcaa aacactacg attgttcag cagcaaacc 
      acgatggtt tcgaatagt caacagtta cagaatttc 
      cagaataat ggagggaat cgaacgact gtgaatgtg 
      gatcagaat tttatcatc gcgaatgat aataagcaa 
      gtgcaggcg cttgttcag gttcagagg gagagtccc 
      gggacgaat tatagacag tcggtgtcg acgcaaaca 
      gcggttaat cagaatgca cagacagtg accacgttt 
      tgtcagacg tccacgctg tgtgcgggg agtcccccg 
      gatactaca acgcatagt ccgttggcg tcgggagga 
      cagagtcct gcaacggcc gatacgacc acacatacg 
      ggaagtaca gacgatggg ctgtcgcca gcgccgtcg 
      aattgttcg gtgacgtgc gatagtgca gggagacag 
      ccggcttgc acgatggcg atggtgcac tgcatatcc 
      agcagcgag cccgattcg gctgatctg aacacccaa 
      ggctctgac catgactgg tcccgagcg tcgtctatc 
      cctcagcaa aaaagcgac ttgtcggag atacatttc 
      cccaaaaaa caaaacact ccgacccac gtggcttac 
      gccgaatcg tcgacaatg atggccggt ttgcatttt 
      atcggcgat caaatgaag gcccaagag gccgtcgtc 
      agttctcac aatttcgat aaaatcgta cagaaacgc 
      aaaagcagc gatactttg ctcgtcatg gaaacccat 
      cacatgcat tcgtcgctt ttcgacgag gaggaagat 
      aatgagaaa cgtgaaaag tcagaacat gactttgtt 
      gttggcgat ttggtttgg gggccggct aaagcaagt 
      ccagcttgg cctggcaaa ataatacaa gtcgacggg 
      aacgatgtt tcggttaaa tggttcggc agcgaaaaa 
      ttaattaca aaattagac agaggttcc ttgcagaca 
      ctcactgaa ggtctcgat gctcaccac caagcgcgc 
      aaaaaatgc agaacatct cgcaaactg aattctcat 
      ctggagaag gccatccaa gaggccatg gctgagctg 
      gataaggac ttggagcaa gagacgaag gaggtgaag 
      cagcagaag aagaaagcg caccgagtg agaagcggt 
      catcgctag
    mRNA sequence
    • >TC000591 RA
      ggagtgtga gatggtttc cattttcag tgtcgagtt 
      ttattacgc ggcgcataa cattagcgc gaataaatc 
      cctataaaa taggtgttg taagttgtt aaaaccgcg 
      ttttgtctt cgcctacaa ctcgtagta gctaaaaat 
      gcccaaact gaaattatt tcagccgaa tggaacgta 
      atttgttgg aagcaggtg ttgggttaa ccacggtga 
      aattaaaac gtagaatat cctgctcaa atcgatgtt 
      ttaagagat tgtaatggt aattatcgc tggtcgtcg 
      ctattctca gtacacaga gacgagatg cagcggggg 
      ctagcaggg ggctcgacg cgagcgccg ttatgctgt 
      cacgatggc aggaaagcc tgagctgtc gcccgcccc 
      cggcgtcca gaatgtggt agtgtacgt ttcgaacga 
      caacttcgc ccaagcgta cgccgtcgg ggggcagaa 
      tgcgcccca gtacggccc cccgaagcc ctaccccga 
      gaagcggcc cgactaccc cgagggcta cttcgtctc 
      gtccaacgg ctacggata cgaggagcg cccggccaa 
      ggagtgcga ctacgaccc ggcctatgt gacggtcat 
      gggggccga ggggccccc gcagagtgt gcggtgtga 
      ttcagtgcg gagcgagac ggccgagtc gagctgcag 
      cagcttgag ctcggccga cgaggggat ggtgctagt 
      acagaacaa tcaaccaga gatggtggt gtatgatcc 
      cagtgttag tgttagacc cggtgtcgt gtttgtggg 
      ggccggcgt ccaccacca ccccaatgt cgccgccac 
      cgccgccgc caacctacc cgccgccca acagccctc 
      catcactgt gccctacgg ctggaagag gattctcaa 
      caacggatc catcatgta cataagccc aagtaacac 
      agctttaag ttctccgga acaagtcaa agaatatct 
      cctcacgtc gggcacttg caagtgtgg attagaatg 
      tcatttcaa atatgacaa cacttttaa tttcgatcc 
      taaggtggc cagtaaacc gtggacgct atcaccgga 
      caccaatac aggggacct gaccaaatt gtgcaacca 
      caagagaaa aatcatcgc catggcctc gttggagtg 
      caagccccc ggaccccgc cgccaaaat gcgaaaaga 
      ctgcaacaa taagaaaac caagcgaaa agtcggcat 
      gccttacgc cggttgtgt ttctttatc gcaaatttt 
      cactcgtcc ggacaaact cgctttgag tcaacagca 
      tttcaacaa ggaaggtct cggaaatca agctcaggt 
      gtggcctca gtcaccgac tagcagctc tcaaatggg 
      taatcgtct gcagaatta ccccgagag ccagcagat 
      ggtccgata tcaaaacca ggagcaaca gaatcgaat 
      cgttaacgg ttcgcccat ggtggtcgg tgataacag 
      taccatgat ggtacccca acaacagtc caacatatc 
      gcaacagat ccaccagaa ccacaatgg tcaaaatcc 
      cagcgttcc tcagcagca cattatcgg gcccaacgg 
      ccaagtgat aagtctcca aggggccct taactcgaa 
      tcaggtcaa taatggagt ggtcataca gggtcaaaa 
      cgtcgttcc taatccaaa tcaatcccg agttttcat 
      caacagcca accaagcga acccagtgg accaggccg 
      gcccaacat ccagatgat tcccgtagg agtcacgaa 
      tcaaagaga tgcccaaaa ccgcctcca ccacttcta 
      caacaacca acaataccc acagataca atacgacgc 
      gtcgaagca aatgcgacc gacatttat gcccggcca 
      caaccaaat gaacggttt tcgcccgca aaataatca 
      gattatgca agcgccggt tatgattaa caaccatca 
      aaagatgcc atggcctaa tcgcattca aaattcgcc 
      caatgccta cgaaagagt gccgccgct gcatccaca 
      cacaccatc gcccactat gtggcaaga cgaggtcaa 
      acggaaaaa ggtcaaatt ggggaaaat cgttaaaaa 
      tcggccgta tcatatgat ggattcggc gttgcatgc 
      accctgtcc caacattga cgtgcggca aataccagc 
      cgaaaacgg ccgacaggt tattatcaa ccagaattc 
      ggccagtcc ctcgtttat ggaggaccc cagtgggta 
      tttagccca gcagacggc cctgctcaa taataccat 
      caacagaca aacaggggt gaacgcgtg cgcgggcat 
      ggtgtgcag cagcccgca gcagcccca cctaacacc 
      accgaattc gcaaacaaa cgaggtgcc tttacccag 
      caacgtggt gatatctca tatgaaaca aacgcaaaa 
      cattacaaa tcgaaccaa tcaaacgtt acacgtagt 
      aaaaagtca agttcctca agtaatgtc ccagcagta 
      taatcaaat gatggcggc tcacgccga cagtagcgt 
      caccaacga ccaatgtca aaattgcga cagtcataa 
      aggccattc gagacctaa cagtcaacc tggaactcc 
      gagttcgtc ggctattga cgatccggt gacatcatc 
      gacttattc cgacaaaca aaataatca atcaccgga 
      ttcaagacc gattcaggg cggtactgt tagtactag 
      caatgtatc gccattgga tggatcaca atcagatcc 
      acctacacc taacccaca cactccaac gcctcctag 
      gaatgataa tatgccata tgctgtcta ccaatcgcg 
      tgaggtttg cagtacgaa ttacccacc gaaaagtga 
      ctgttgttc gaaaatgaa cacgccgta tatttcgag 
      tatagttac gacgatggc aagtggacg aacagttgg 
      tagtaatac cataacgtc ggttttggc cggtcgtgc 
      caacactgc cacagtttc agtcaacag tcctgctaa 
      cctcccagc atccacaac cccaacaac agtcacaca 
      aaccgtttc aaaatcccc acttgaaat ggtccaaag 
      cgtcgtcag tagcataca agtccctca gtctcaaac 
      caatcccca aatctctcc accacatat cgtcaaaca 
      ttcccccac cgggttacc tcctggcca tatccttgt 
      ctcaagcgg cggccagtt aataatggc aaacaccgg 
      aaacagcca atcgggcgt tatgccacc cccaccccc 
      caaactagt ctccaatca aaccgcaat gccgccaat 
      gtccgtctc accaatgat cacaaatgt cactgcagc 
      ggtcagtca ggtcatacc ggcagtagc tcagcaagt 
      tttaggcca acaaaccgt tttagtgaa cgccttacc 
      cacaccttt tgtcttaca aggcggagt cacaatgac 
      catggacgg gtccatggt cggccaaaa catgcaatt 
      acctcaaat cgtcgccgg aaatgtgat acaacaaca 
      aatccaact ggataacag tgacccacg acgtaccac 
      cgcgctact ctcacccga aaccaagaa aaaaggaaa 
      gaaacgaaa aatgtcaca aacagtggc caacatgct 
      tcacatagc tgcccagca aaactccgg cgttgtgat 
      gtcacaaca aggcttccc gcagcaaat tcaaatggc 
      tcatagtcc gcaaggtct agccacggg cccagtgat 
      gcaagcctt gaccatagt ccccagcaa aacaggggg 
      acccccaca gatcgtaat gaacgggca acccatgac 
      caacaccag tccccaact cattgcaaa ctcgcaacc 
      ggcgcaaca aatcaacct ccttcagcc ggtgaatct 
      catcaatgg caccaccgg agtggtgca aaatttccc 
      cacgattca acaattcat agtcccgaa tttaggcgg 
      catggttat gaacccgga cggcaccgc cactatatt 
      acaggacac ttcaaatat cggcatgca gttacaact 
      acagaacgt taacggaca aaacgtcct gactccggt 
      ccaaaacaa cggaatgtt caacggggg tcagagtat 
      tctagcagc gagtccggc cggcatggt cattagggc 
      ccccacctc gcaggggaa attaatcca gcagcagca 
      gcacagtcc aggtgccca gtttttgtc acccaacgg 
      gggccagtt tgtcgtcaa tgggacaca attcagcgg 
      ccagttgag cccgcttgt ggctagtgt gagtccgag 
      tcagcaagt tacctttgg gacgtccac gccgcagat 
      tagaccaac tcagggtca acaagaatt tatacagtg 
      tggtcaaat gggccagac tttgatggt tccgtgtgc 
      accaacggt cgccgtttc ctcctcgca aaacacgac 
      ttttgtcca gcaaaacac tacgattgt tcagcagca 
      aaccacgat ggtttcgaa tagtcaaca gttacagaa 
      tttccagaa taatggagg gaatcgaac gactgtgaa 
      tgtggatca gaattttat catcgcgaa tgataataa 
      gcaagtgca ggcgcttgt tcaggttca gagggagag 
      tcccgggac gaattatag acagtcggt gtcgacgca 
      aacagcggt taatcagaa tgcacagac agtgaccac 
      gttttgtca gacgtccac gctgtgtgc ggggagtcc 
      cccggatac tacaacgca tagtccgtt ggcgtcggg 
      aggacagag tcctgcaac ggccgatac gaccacaca 
      tacgggaag tacagacga tgggctgtc gccagcgcc 
      gtcgaattg ttcggtgac gtgcgatag tgcagggag 
      acagccggc ttgcacgat ggcgatggt gcactgcat 
      atccagcag cgagcccga ttcggctga tctgaacac 
      ccaaggctc tgaccatga ctggtcccg agcgtcgtc 
      tatccctca gcaaaaaag cgacttgtc ggagataca 
      tttccccaa aaaacaaaa cactccgac ccacgtggc 
      ttacgccga atcgtcgac aatgatggc cggtttgca 
      ttttatcgg cgatcaaat gaaggccca agaggccgt 
      cgtcagttc tcacaattt cgataaaat cgtacagaa 
      acgcaaaag cagcgatac tttgctcgt catggaaac 
      ccatcacat gcattcgtc gcttttcga cgaggagga 
      agataatga gaaacgtga aaagtcaga acatgactt 
      tgttgttgg cgatttggt ttgggggcc ggctaaagc 
      aagtccagc ttggcctgg caaaataat acaagtcga 
      cgggaacga tgtttcggt taaatggtt cggcagcga 
      aaaattaat tacaaaatt agacagagg ttccttgca 
      gacactcac tgaaggtct cgatgctca ccaccaagc 
      gcgcaaaaa atgcagaac atctcgcaa actgaattc 
      tcatctgga gaaggccat ccaagaggc catggctga 
      gctggataa ggacttgga gcaagagac gaaggaggt 
      gaagcagca gaagaagaa agcgcaccg agtgagaag 
      cggtcatcg ctagatttt atatcaaat taataataa 
      aaatggacc aacatcgaa tgaaatgtg tataaatgt 
      acgtagcga gttgatgaa aaacgtaga agatacatt 
      tttactaac gcttcttta atgtaaaaa tataatcat 
      agaccgatg ttataactg gtattgatg attcctaga 
      tgataatat aatcgtata tgagatatt tttcaaatg 
      cctttttta aactagcgg tatggcacc ataataagc 
      ctaatcttt aaaatttgt actaacaca tattcaatt 
      tatgtaaaa aaatcataa cggaaattg ggctttcat 
      gatgtttgt ctaattagt tatgtaaaa gatacatag 
      agcttagac tttatccaa aatatattg cgggtctta 
      taaaagtaa acaaatcat ttatgaaac gacacctac 
      aagttttgt atttctagt caatgtttt aagagctga 
      cagttaaac gacaataag atgtaccat tggtttaaa 
      gcgtttatt tttcggtac tgtgatatg ctggaaata 
      tatccaata tgggtttgt catgggttt attgattta 
      gggaacttg ccatgtttc gaatcggct cgaagtgag 
      caagccata ttaggactt ggttttaat tacactgca 
      gtgtactgt ataggtttt atcaattgc attttagta 
      ctaacaacc ataatcaaa ttatgaaac aatgcttac 
      attaagtag ttacagtta agtgaaagt tgtacatta 
      tatttttat attatgtat ttattgttt atagttctt 
      tccggtctt agttttaag taggatgtt aatttattt 
      cgtgtgtga ctcgaaagt gacactttc gaggctaag 
      tgtcgctct cgagtgcca caataagtt gaaatttat 
      ttaacccag tgttttact gcgtctgca tcacaataa 
      tctctagat taaacaaag tgaaatagg taaccagag 
      gccgtccgc tttaaagca aatgcaatg tggcatttc 
      tcgcatcac ccttagagt ggacaaagc attttcaat 
      ttttttcct tgattatgt attatttat tttcgtctt 
      atgtatata tttttatag gtacctgtt ctgtgtcac 
      agatatgga ttgtataac cggtgtcat tttttgtat 
      tgtgtttat ggagtggtt gtgataatt actggaaaa 
      taagagaaa cgaaactac tctgtaagc acgatttcg 
      tactataat ccagtgtta gagctacgc gtcacatga 
      atgaagcat gtgcggtag ctgtgtctg tttctgtaa 
      atatattgt attaatact ttactgttt ggcctgcta 
      tgttgtggt tgaacattt ctccagccc aataaatac 
      tggattaga ca
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