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Gene information
General information
TC000590 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 3834683..3837843
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000590
    iBeetle screen iB_00132
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    potentially confused/crosscontaminated with : iB # (comma separated) iB 147
    Pupal morphology
    potentially confused/crosscontaminated with : iB # (comma separated) iB 147
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000590 RA
      MVKETKFYD ILGVKPGCT QDDLKKAYR KLALKYHPD 
      KNPNEGEKF KQISQAYEV LSDPEKKRI YDQGGEQAL 
      KEGGVSGGF SSPMDLFDM FFGGGGFGG GRGRRRERK 
      GKDVIHQLN VSLEELYKG TVRKLALQK NVICDKCEG 
      RGGKKGAVE TCPTCRGSG MQVQIQQLG PGMIQQIQS 
      MCSDCRGQG QRINPKDRC KQCQGKKVT RERKILEVH 
      VDKGMVDGQ KIVFNGEGD QEPELEPGD IIIVLEEKE 
      HPVFRRSGL DLIIRLELQ LVESLCGFQ KVIRTLDDR 
      DLVITSLPG EVTKHGDVK CIMNEGMPQ YKNPFEKGR 
      LIVQFLVQF PDKLPPEVI PALENALPP RPEIMIPDQ 
      AEECILLPF DVDKQDSRR RQNRNVYDE DDEMHGPGQ 
      RVQCAAN
    CDS sequence
    • >TC000590 RA
      atggtaaaa gagacgaaa ttctacgac atcctgggc 
      gttaagccc ggctgcacc caggacgac ctgaagaaa 
      gcctaccga aaactcgcc cttaaatac caccccgac 
      aagaacccc aatgaaggg gagaagttc aagcaaatc 
      tcgcaagcg tacgaggtg ctgtccgac ccggaaaag 
      aagcgcatt tacgaccaa ggcggcgaa caggccctc 
      aaagagggc ggcgtctcg ggcggcttc tccagccct 
      atggacttg ttcgatatg ttcttcggc ggaggcggc 
      tttggtggc ggcagaggc cgacgtcgc gaacgtaaa 
      ggcaaagat gtcattcat caacttaat gtttctctg 
      gaagagttg tacaaagga accgtccgg aaattggct 
      ctacagaaa aacgtcatc tgtgataag tgcgaaggc 
      aggggtggg aagaagggg gctgtggag acgtgtccc 
      acgtgtcgg ggctccggc atgcaggtg caaatccag 
      cagttgggg ccaggcatg atccaacaa atccagagc 
      atgtgttcg gattgccga ggccaaggt caacgcatc 
      aatcccaaa gaccgttgc aagcaatgc caaggcaag 
      aaagtgact cgcgaacgc aaaattctc gaagtccac 
      gtggacaag ggcatggtt gacggtcaa aaaatcgtt 
      tttaacgga gaaggggac caagaacca gaactggaa 
      cctggcgat attataatt gtgcttgag gagaaagaa 
      catcccgtt tttcgaaga tccggcttg gatctcata 
      atccgcctt gagttgcag ttagtagag tcgctttgc 
      ggattccag aaagtaatc aggactttg gacgaccgc 
      gatttggtt atcacgtcg cttccgggc gaggtcacg 
      aaacacggg gatgttaag tgtattatg aacgaggga 
      atgccgcag tataagaat ccgttcgag aaaggacgg 
      cttatcgta caatttttg gtacagttt ccggataag 
      ttgccacct gaggtgatt ccggcgctg gagaatgcg 
      ctaccgcct cggcctgaa attatgatc ccggatcag 
      gcggaagaa tgcattttg ttgccgttt gatgtggat 
      aagcaggac tcgaggagg aggcagaat aggaatgtt 
      tatgacgaa gatgatgag atgcatggt ccgggacag 
      cgggtccaa tgtgcggct aactaa
    mRNA sequence
    • >TC000590 RA
      aacctttta agtggaata cgtgactta aaatcaaaa 
      attctatta aaaggcgcc gcttttcga ataagtaag 
      ctgagtagg cctgcagag ggcgtcctg tcaatttct 
      agaattcgt cattcgtta cgtcttcga cctttctct 
      tcgtcgaat tttccagaa cctcgtggc tgggagttt 
      tatccctga acacgcgct ctagaacca gtctttgct 
      ttgtgctgc gtcagtgtg caaacttaa aaaatccac 
      gagttgtaa tccccgttc cagaaatat tgattattt 
      ctgaggact acaaccatg gtaaaagag acgaaattc 
      tacgacatc ctgggcgtt aagcccggc tgcacccag 
      gacgacctg aagaaagcc taccgaaaa ctcgccctt 
      aaataccac cccgacaag aaccccaat gaaggggag 
      aagttcaag caaatctcg caagcgtac gaggtgctg 
      tccgacccg gaaaagaag cgcatttac gaccaaggc 
      ggcgaacag gccctcaaa gagggcggc gtctcgggc 
      ggcttctcc agccctatg gacttgttc gatatgttc 
      ttcggcgga ggcggcttt ggtggcggc agaggccga 
      cgtcgcgaa cgtaaaggc aaagatgtc attcatcaa 
      cttaatgtt tctctggaa gagttgtac aaaggaacc 
      gtccggaaa ttggctcta cagaaaaac gtcatctgt 
      gataagtgc gaaggcagg ggtgggaag aagggggct 
      gtggagacg tgtcccacg tgtcggggc tccggcatg 
      caggtgcaa atccagcag ttggggcca ggcatgatc 
      caacaaatc cagagcatg tgttcggat tgccgaggc 
      caaggtcaa cgcatcaat cccaaagac cgttgcaag 
      caatgccaa ggcaagaaa gtgactcgc gaacgcaaa 
      attctcgaa gtccacgtg gacaagggc atggttgac 
      ggtcaaaaa atcgttttt aacggagaa ggggaccaa 
      gaaccagaa ctggaacct ggcgatatt ataattgtg 
      cttgaggag aaagaacat cccgttttt cgaagatcc 
      ggcttggat ctcataatc cgccttgag ttgcagtta 
      gtagagtcg ctttgcgga ttccagaaa gtaatcagg 
      actttggac gaccgcgat ttggttatc acgtcgctt 
      ccgggcgag gtcacgaaa cacggggat gttaagtgt 
      attatgaac gagggaatg ccgcagtat aagaatccg 
      ttcgagaaa ggacggctt atcgtacaa tttttggta 
      cagtttccg gataagttg ccacctgag gtgattccg 
      gcgctggag aatgcgcta ccgcctcgg cctgaaatt 
      atgatcccg gatcaggcg gaagaatgc attttgttg 
      ccgtttgat gtggataag caggactcg aggaggagg 
      cagaatagg aatgtttat gacgaagat gatgagatg 
      catggtccg ggacagcgg gtccaatgt gcggctaac 
      taattttcg agtattatt aagggactt tgatataga 
      catcttgat agcatagac gaaattgtt ttagtaggc 
      aattttatt aagtaattg taattattt aaacataaa 
      atagggaca gcagaaggt tccgttcat gctgaatgt 
      atgtggctt tttgttgga attatttac ggtcttcta 
      attgtgaaa attgatcag tttcgtggt atttatttt 
      tttatttga agcgtctta ccgagatat tttgaataa 
      atgtttctt tatgtt