| General information | |||
| TC030994
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 9580333..9598953 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC030994 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00131 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC030994 RA
MGFTVQFLL GTFLVLLCC SSIRSTQVK NEEETTEKT QIEKITEAN LESNKPKTE NSTENSVGS KLQKPEEIG RSIDIEKYV SKEFHNKNN VYTLPTSPD TEKYIDPDY DFHATTLKH HEHEEFNYE SELTTPKSS IFDHRYKEA VESVDQKLE HIDDSHEVS AETDSKESV DLEKLYEET KKNDTKADL NYINFKNVV ANDTGSKNS LRKNVVLES GTNLNFQSS YNHTTIDEN DSGNNTIPK EDKKTESTT LSNVIAVKG ELEKAESST VKATLTTRK SLVLTKPPS SRNSSHISI LSNKIETET VKAINPTDK PKGDKENSS ESYTQKTPI DITKRGSVK YAHTISTEP TTVSNNIKK SEQLLQENQ VIPVHVEAP PTAINSTTE TPSTTTPET ETRITYSIE TRIKSVSVG TQPPTETTT DAQTTETTF LSDSTAPTT VTTENPETT EPLTTTEIT ITTENAVRT SKGLNFGEN VSTIPTEVD VTTTEKMFE TTTEEMVPT NSLPQMFSE KMFTTEKIA PKMFSAMGL TTTEKMFQA TQETTERSE PTRMFSDVA QTTTATQET TEKIEPTNH TLKMFPDVA PTTTEKMFT ATQETTERI EPTNHIPKM FSDVTPEKI PATTERITP TNSIAKMFS DVIPETTTE LEPTVSTNR TNVPVFRNV PTEPSEPEI TENFTITSD SYESSTGAP DDSSGKTAA IAISSIAAV CLIVIAGLL VFMRKRQKR FNYGQRCRP VSLDDYSMD NVSVYNSVR RKGMLRGSK RSYGNPAFE DPVTVTHPL NFPALGKFA TNLEDIKAE FEEIPQITA RTTELPEGC ETKNRYANV IPLPETRVL LNLIDGYPT SDYINANYV TGPKNTKGY YIACQAPMQ NTVDDFWRM IWEQQCKVV LMVTHLFEN GVEKCVDYL PPSEVLDCH RLFGDFQVT LKKREVKDK YIISSLQLK NMVSNSWRE VTHFWYMGW PEKGVPTEA NSLIAFLIE ARSYMKSSS MDRKDVTNG NLANGGAKN EVNPVVVHC SPGTGRTGV VIACDIAIR EFEQTRLVD VPRIVYKIR RDRASAVQT KEQYAFIYK VITLYATKL TGDNGAFQL HENGVKEDT ISDLKPTAN YPIQKRKKS LVQLTREAL PRLENYRNS RRAAKRPSL GELHGGEDV VKREPNGTS EEEVPAPGS HDGIKLGWI QGVLIPCLL NIFGVMLFL RLSWVVAEA GIGQSLVII GISAVVCII TTLSLSAIS TNGEVKGGG IYYIISRSL GPEFGASVG IVFAFANAV SASMNTIGF CNSLSDLLG RYGLKIIDS GINDARIIG IVAILVMIL ICAIGMEWE SKAQNILIV AIVAAIVDF LVGAIIGPL SDEKQAQGF TGFNYTTFA TNWGPDYRR SEGMDYDFF QVFAIFFPS VTGIQAGAN ISGDLKDPA SAIPKGTFL ALVISMVTY AIFVMVAGA AAIRDASGS VGDLVNGTL SGCSPDCRY GIFNNYQMM EIMSAWGPL IYIGCWAAT LSTALTNLL SVPRLIQAL GIDRIYPGL IYFSKGYGK AGEPYRGYI LTFLVSAAF LLIGELNAI APLISNFYL ASYALINFC TFHAALIKP LGWRPTFKY YNTWLSLAG FISCVVIMF LINWQSSLI TFGVIIALY LLVVYRKPD VNWGSSTQA QTYKTALTT AHRLINIGE HVKNYKPQI LVLCGSPSS RPALIDFGN LITKNNSLL IIGDVTEAR LQHRARSAR IQNVYSWLR INKIKAFYS ILDNTQFEN GAKALLQTA GIGKLCPNV LMMGYKNDW RTCPREELM AYFNVLHAA FDHRLAVTI LRLKDGLDY SKLMGEDDG SPSEGTLSQ QPTMDLRGF ATSSMMHAD SNLSLADLG KSDTVTSKN TINGVKNGH GLLNSVFAK KQKVADSTP DKTSVQHIP NFAMENMTL FRNKQNKGN IDVWWLYDD GGLTMLLPY IISTRQHWA NCKLRVFAL STNKNEIEE EEKNMAFLL SKFRLNYSS LKIVSLSDK PQEAMIKFF NDMISDFRT NEDLDDESK VSEAEIASL QDKTYRQLR LRELLLEHS SEANLIVMS LPMPRKGKL SAPLYMAWL EALTRDLPP YLLVRGNQQ SVLTFYS
- >TC030994 RA
atgggcttc acggttcag ttcttgcta ggcacgttc ctggtcctt ttatgctgc tcctcgatc cgaagtacc caagttaaa aatgaggaa gaaacgacc gaaaaaacc caaatcgaa aagataact gaagcgaat ctcgaaagc aacaaacca aagactgaa aatagtact gaaaatagt gtaggaagc aaattgcaa aaacccgaa gaaattggc cgatcgatt gatatcgaa aaatacgtt tcgaaagaa tttcacaat aaaaataac gtttacact ttaccaacc tcgccggat actgagaaa tacatagat ccggattat gatttccac gctacgact ctgaagcat catgaacac gaggaattc aactacgag tcggagtta acaacgccg aagagttcg attttcgat cacaggtac aaggaagca gtggagagt gttgaccag aagttggaa catatcgat gattcgcat gaagtttcg gctgaaacc gactcaaag gaaagtgtc gacttggag aaactttac gaagaaacg aagaagaac gacaccaaa gccgatctg aattatatc aactttaaa aatgtggtt gctaatgat acgggtagt aagaactct ttgcggaag aatgtggtg ctcgagagt ggaactaat ttgaatttc caatcatcc tataatcac acaacgatc gatgaaaat gatagcgga aataacacg attccgaaa gaagataag aaaactgaa agcacgact ttgagcaat gtgattgcc gttaaaggc gaattggaa aaagccgaa agttcgacg gttaaagcc actttaacc acccgaaaa agtctagtt ttgaccaaa cctccgtcg agtcgcaat tcgagccat atctcaatt ttgagcaac aaaattgaa accgaaact gtcaaagcg atcaacccg acagacaag ccgaaagga gataaagaa aattcctcc gaaagctac acccagaaa acgcccatt gacataaca aagcggggc tcagtcaag tacgcacac accatttca accgaaccg acaacagtc tcgaacaac atcaaaaaa tcggaacaa cttttgcaa gagaatcaa gtgattcca gtccacgtt gaagcacct ccaactgcc ataaacagc acaactgaa actccctca accacaacg cccgaaact gaaacacgc attacctac tcgattgaa actaggatt aaaagtgtt tcagtgggg acacaaccg ccgactgaa accacaacc gacgcacaa acaaccgaa actacgttt ttatcagac agtactgcg cccacaacg gttacaacc gaaaatcct gaaacaact gagccccta acaacgact gaaattaca atcactacc gaaaatgca gtgagaacc tcgaaaggt ttaaatttc ggggaaaat gtgtccaca ataccgact gaggtggac gtgacaacg actgaaaaa atgttcgaa actacaacc gaagaaatg gtaccgact aattctctt ccgcaaatg ttctcagaa aaaatgttc acaactgag aaaattgca ccgaaaatg ttctcagct atgggcttg actacgact gaaaaaatg ttccaagcg actcaggaa acaactgag agaagtgaa cccacgaga atgttctca gatgtggcc caaactacg actgcaact caggaaaca accgagaaa attgaacct acgaatcat actctgaaa atgttccca gatgtggcc ccgactacg actgagaaa atgttcaca gcgactcag gaaacaacc gagagaatt gaaccgacc aatcatatt ccgaaaatg ttctcagac gtgactccc gaaaaaatt ccagcgaca actgagagg attacaccg acgaattca atcgcgaaa atgttctca gatgtgatt cctgagact acgactgag ctggaaccg actgtttca accaaccgc acaaatgta cctgttttt agaaatgtc cctactgag ccaagcgag cctgaaatc actgaaaac ttcacaata acttcagat tcgtacgaa tccagcaca ggggctcct gacgacagc agtggtaaa accgccgca attgctata tcttcgatc gctgctgtt tgtcttatt gtaatagcc ggacttctg gtgtttatg cggaagagg cagaagagg tttaactac gggcaaaga tgccggcct gtgagtctc gacgactac agtatggac aacgtctcc gtgtacaac agcgttcgc aggaagggg atgttaaga gggtcgaaa cgctcgtac ggaaatccg gcgtttgag gaccctgtc accgtcact cacccgctg aacttcccg gctttgggg aaatttgca acgaatttg gaagacata aaggccgaa tttgaggaa atcccccag atcacggcc cgaactacc gaacttccc gaaggatgt gagacgaaa aacagatat gcgaatgtg atcccactt ccagaaacg cgcgttttg ttgaaccta attgacggt taccccacc agtgactac ataaatgct aattatgtt acgggcccc aaaaacaca aaggggtat tacatagcg tgccaggcg cctatgcag aacacagtt gatgatttc tggcgcatg atttgggag caacaatgc aaagttgtt ctaatggtg acccatttg tttgaaaac ggggtcgaa aaatgcgtt gattacctg ccgccttcg gaagtgcta gactgtcac cgcctcttt ggcgacttc caagtcaca ctgaaaaaa cgcgaagtc aaagacaag tatatcatt tccagcctc caactaaag aatatggtg tcaaatagc tggcgcgaa gtcacgcat ttctggtac atggggtgg ccggaaaaa ggcgtcccg acggaggca aattcgctc attgcattt ttgatagaa gcccggagt tacatgaaa tcgtcgtcg atggatagg aaggacgtt actaatggt aatttggcg aatggtggc gcgaaaaat gaggtgaat cctgtggta gtgcactgt agtcctggg acaggacga acaggtgtg gtgatagct tgtgatatt gcgatacga gagtttgaa caaacccga cttgttgat gtaccaagg atagtctat aagattaga cgggatagg gcgagcgca gtacaaact aaagagcag tacgcattt atttataag gtgattact ctttacgct actaaactt acaggcgac aatggtgct ttccagtta cacgagaac ggggtgaaa gaggacact atcagtgat ttgaaaccg acagcaaat tatccgatc caaaaacgc aaaaaatcg ctcgtgcaa ctcacacgc gaggcgctc ccgaggttg gagaactac aggaattcc cgaagagcg gccaaaagg ccctcgctg ggcgaatta cacggcggc gaagacgtc gtcaagcgt gaacccaat ggcacgtcc gaggaggag gtcccagcc cctggcagc cacgatggc atcaaactg ggctggatc caaggcgtc ctcatcccc tgcctcctc aatatcttc ggtgttatg ctattttta cgtctttcg tgggtcgtt gcagaagct ggaattggc caaagcttg gtcataatc ggcatatca gccgtggtt tgtatcatc accaccctc tccttgtcg gcaattagc acaaacggc gaagtcaaa ggaggcgga atttactac atcatttcg cgatcattg ggccccgaa ttcggggct tccgtcggc attgtcttc gcgttcgct aacgcggtc tcagcctcg atgaacacc atcggtttc tgcaactcc ttgagcgat ctcttgggc cgttacggc ctaaaaatc atcgactcg ggcatcaac gatgcccgc attattggc attgtggcc attttggtc atgattttg atttgtgcc atcgggatg gagtgggaa tccaaagcc cagaacatc ctaatcgtg gcaattgtg gccgctatt gtggatttc ctcgtcggg gccataatc gggcccttg tcggacgaa aaacaagcc cagggcttc accggcttc aattatacg acttttgcg acgaactgg ggcccggat taccgccgc tcggagggc atggactat gattttttc caagttttt gccattttt ttcccttcg gtgaccgga attcaggcc ggggcgaac atttccggg gatttgaag gatccggcg tcagcgatc ccgaaaggg acgtttttg gctttggtg atttcgatg gtgacttat gcgattttt gtaatggtt gccggagcc gcagcaatc cgggacgcc agtggcagt gtcggggac ctagtcaac ggaacgctt tccggttgt tcccccgac tgccggtac gggattttc aacaattat cagatgatg gagatcatg tcggcttgg gggcccctc atctacatc ggctgctgg gcggccact ttaagcacg gccctgacc aatctcctc tcggtcccg agattaatc caggccttg ggaatcgac aggatttat ccaggatta atctacttt tccaaagga tatggcaag gccggggaa ccgtatcga ggatacatt ttaacgttc ctggtatcg gcggctttt ctcctaata ggcgaattg aatgcaatc gcaccgttg atttccaat ttttatcta gcctcgtac gcccttatc aatttttgc actttccat gccgcgcta atcaaaccc ttggggtgg agaccgacg ttcaaatat tacaatacg tggctaagc cttgccggg ttcatcagc tgcgtcgtt atcatgttc ttaattaat tggcaatca tccctaatt actttcggc gtaattatc gccttgtat ctcttggtg gtttaccga aagccagat gttaattgg ggctcgtcc actcaggcc cagacgtac aaaacggcc ctgacaacg gcccaccgc ttgataaac atcggggag cacgtaaaa aattacaaa ccgcaaatt ttggtgctt tgtggctcc ccgtcctca cggcccgct ctaatcgat tttggcaac ttgataacg aaaaacaat tcgttgctg atcatcggg gacgtgaca gaggcgagg ctgcagcac cgggccagg tcggctcgc atccaaaac gtctactcc tggctcagg attaacaaa atcaaagcc ttttactcg attttggac aacacgcag ttcgaaaac ggggccaag gctttgctg caaacggcc ggcatcggc aagctttgc cccaacgtc ctcatgatg ggttataag aacgactgg aggacttgt ccccgtgag gaattaatg gcttatttt aatgtttta catgcggcg tttgaccac cgccttgct gtgacaatt ttgcgtctg aaagacggt ctggattac agtaaactg atgggcgaa gatgatggt tcgcccagc gagggcact ctgtcccag caacccaca atggatttg cgaggtttc gccacaagt agtatgatg catgctgat tcgaattta agtttagct gacttagga aaatcagat actgttacg tctaaaaat acaataaac ggggtcaaa aacggtcac gggttgtta aattcagtc tttgccaag aaacagaaa gtcgctgat tcgaccccc gacaagacc agtgtgcaa catatcccc aatttcgca atggagaat atgactctg ttccgaaat aagcaaaac aagggaaac atcgatgtt tggtggttg tatgacgat gggggcctg acgatgctt ttgccgtac attatttca accaggcag cattgggcc aattgcaag ctgcgagtt ttcgccctc agtacaaac aaaaacgag atcgaagaa gaggagaaa aatatggcg tttttgttg tcgaaattc cgactgaat tactccagt ttgaaaatc gtttcgctt tcggataag ccacaagag gcgatgatt aaatttttc aacgatatg attagcgat tttaggacg aatgaggac ctcgatgat gaatccaag gtttccgaa gccgagatt gcctcgctt caggataag acgtaccgg cagttgaga cttcgtgag ttgctgtta gagcactcg agtgaagca aatttgatt gtcatgtct ttaccgatg ccgaggaaa ggcaaatta tcagcgcct ttgtacatg gcatggttg gaagcgctg acccgggat ttgcccccg tatttgcta gtgagaggg aaccagcag tccgttctc actttttat tcctaa
- >TC030994 RA
cccactcag cgtgttcaa agcaaacat tttatctcc acctctgga caaaaattc gaacggtgt gataacacc aaggaaatt tgtgcgtca agtgtgtgt gtggtgtgt ttttatcga ttgtagtcc ttgctcaaa aatcggata tctgctatg ataatcgag ttttggtga caacaatgc caacattat gggcttcac ggttcagtt cttgctagg cacgttcct ggtcctttt atgctgctc ctcgatccg aagtaccca agttaaaaa tgaggaaga aacgaccga aaaaaccca aatcgaaaa gataactga agcgaatct cgaaagcaa caaaccaaa gactgaaaa tagtactga aaatagtgt aggaagcaa attgcaaaa acccgaaga aattggccg atcgattga tatcgaaaa atacgtttc gaaagaatt tcacaataa aaataacgt ttacacttt accaacctc gccggatac tgagaaata catagatcc ggattatga tttccacgc tacgactct gaagcatca tgaacacga ggaattcaa ctacgagtc ggagttaac aacgccgaa gagttcgat tttcgatca caggtacaa ggaagcagt ggagagtgt tgaccagaa gttggaaca tatcgatga ttcgcatga agtttcggc tgaaaccga ctcaaagga aagtgtcga cttggagaa actttacga agaaacgaa gaagaacga caccaaagc cgatctgaa ttatatcaa ctttaaaaa tgtggttgc taatgatac gggtagtaa gaactcttt gcggaagaa tgtggtgct cgagagtgg aactaattt gaatttcca atcatccta taatcacac aacgatcga tgaaaatga tagcggaaa taacacgat tccgaaaga agataagaa aactgaaag cacgacttt gagcaatgt gattgccgt taaaggcga attggaaaa agccgaaag ttcgacggt taaagccac tttaaccac ccgaaaaag tctagtttt gaccaaacc tccgtcgag tcgcaattc gagccatat ctcaatttt gagcaacaa aattgaaac cgaaactgt caaagcgat caacccgac agacaagcc gaaaggaga taaagaaaa ttcctccga aagctacac ccagaaaac gcccattga cataacaaa gcggggctc agtcaagta cgcacacac catttcaac cgaaccgac aacagtctc gaacaacat caaaaaatc ggaacaact tttgcaaga gaatcaagt gattccagt ccacgttga agcacctcc aactgccat aaacagcac aactgaaac tccctcaac cacaacgcc cgaaactga aacacgcat tacctactc gattgaaac taggattaa aagtgtttc agtggggac acaaccgcc gactgaaac cacaaccga cgcacaaac aaccgaaac tacgttttt atcagacag tactgcgcc cacaacggt tacaaccga aaatcctga aacaactga gcccctaac aacgactga aattacaat cactaccga aaatgcagt gagaacctc gaaaggttt aaatttcgg ggaaaatgt gtccacaat accgactga ggtggacgt gacaacgac tgaaaaaat gttcgaaac tacaaccga agaaatggt accgactaa ttctcttcc gcaaatgtt ctcagaaaa aatgttcac aactgagaa aattgcacc gaaaatgtt ctcagctat gggcttgac tacgactga aaaaatgtt ccaagcgac tcaggaaac aactgagag aagtgaacc cacgagaat gttctcaga tgtggccca aactacgac tgcaactca ggaaacaac cgagaaaat tgaacctac gaatcatac tctgaaaat gttcccaga tgtggcccc gactacgac tgagaaaat gttcacagc gactcagga aacaaccga gagaattga accgaccaa tcatattcc gaaaatgtt ctcagacgt gactcccga aaaaattcc agcgacaac tgagaggat tacaccgac gaattcaat cgcgaaaat gttctcaga tgtgattcc tgagactac gactgagct ggaaccgac tgtttcaac caaccgcac aaatgtacc tgtttttag aaatgtccc tactgagcc aagcgagcc tgaaatcac tgaaaactt cacaataac ttcagattc gtacgaatc cagcacagg ggctcctga cgacagcag tggtaaaac cgccgcaat tgctatatc ttcgatcgc tgctgtttg tcttattgt aatagccgg acttctggt gtttatgcg gaagaggca gaagaggtt taactacgg gcaaagatg ccggcctgt gagtctcga cgactacag tatggacaa cgtctccgt gtacaacag cgttcgcag gaaggggat gttaagagg gtcgaaacg ctcgtacgg aaatccggc gtttgagga ccctgtcac cgtcactca cccgctgaa cttcccggc tttggggaa atttgcaac gaatttgga agacataaa ggccgaatt tgaggaaat cccccagat cacggcccg aactaccga acttcccga aggatgtga gacgaaaaa cagatatgc gaatgtgat cccacttcc agaaacgcg cgttttgtt gaacctaat tgacggtta ccccaccag tgactacat aaatgctaa ttatgttac gggccccaa aaacacaaa ggggtatta catagcgtg ccaggcgcc tatgcagaa cacagttga tgatttctg gcgcatgat ttgggagca acaatgcaa agttgttct aatggtgac ccatttgtt tgaaaacgg ggtcgaaaa atgcgttga ttacctgcc gccttcgga agtgctaga ctgtcaccg cctctttgg cgacttcca agtcacact gaaaaaacg cgaagtcaa agacaagta tatcatttc cagcctcca actaaagaa tatggtgtc aaatagctg gcgcgaagt cacgcattt ctggtacat ggggtggcc ggaaaaagg cgtcccgac ggaggcaaa ttcgctcat tgcattttt gatagaagc ccggagtta catgaaatc gtcgtcgat ggataggaa ggacgttac taatggtaa tttggcgaa tggtggcgc gaaaaatga ggtgaatcc tgtggtagt gcactgtag tcctgggac aggacgaac aggtgtggt gatagcttg tgatattgc gatacgaga gtttgaaca aacccgact tgttgatgt accaaggat agtctataa gattagacg ggatagggc gagcgcagt acaaactaa agagcagta cgcatttat ttataaggt gattactct ttacgctac taaacttac aggcgacaa tggtgcttt ccagttaca cgagaacgg ggtgaaaga ggacactat cagtgattt gaaaccgac agcaaatta tccgatcca aaaacgcaa aaaatcgct cgtgcaact cacacgcga ggcgctccc gaggttgga gaactacag gaattcccg aagagcggc caaaaggcc ctcgctggg cgaattaca cggcggcga agacgtcgt caagcgtga acccaatgg cacgtccga ggaggaggt cccagcccc tggcagcca cgatggcat caaactggg ctggatcca aggcgtcct catcccctg cctcctcaa tatcttcgg tgttatgct atttttacg tctttcgtg ggtcgttgc agaagctgg aattggcca aagcttggt cataatcgg catatcagc cgtggtttg tatcatcac caccctctc cttgtcggc aattagcac aaacggcga agtcaaagg aggcggaat ttactacat catttcgcg atcattggg ccccgaatt cggggcttc cgtcggcat tgtcttcgc gttcgctaa cgcggtctc agcctcgat gaacaccat cggtttctg caactcctt gagcgatct cttgggccg ttacggcct aaaaatcat cgactcggg catcaacga tgcccgcat tattggcat tgtggccat tttggtcat gattttgat ttgtgccat cgggatgga gtgggaatc caaagccca gaacatcct aatcgtggc aattgtggc cgctattgt ggatttcct cgtcggggc cataatcgg gcccttgtc ggacgaaaa acaagccca gggcttcac cggcttcaa ttatacgac ttttgcgac gaactgggg cccggatta ccgccgctc ggagggcat ggactatga ttttttcca agtttttgc cattttttt cccttcggt gaccggaat tcaggccgg ggcgaacat ttccgggga tttgaagga tccggcgtc agcgatccc gaaagggac gtttttggc tttggtgat ttcgatggt gacttatgc gatttttgt aatggttgc cggagccgc agcaatccg ggacgccag tggcagtgt cggggacct agtcaacgg aacgctttc cggttgttc ccccgactg ccggtacgg gattttcaa caattatca gatgatgga gatcatgtc ggcttgggg gcccctcat ctacatcgg ctgctgggc ggccacttt aagcacggc cctgaccaa tctcctctc ggtcccgag attaatcca ggccttggg aatcgacag gatttatcc aggattaat ctacttttc caaaggata tggcaaggc cggggaacc gtatcgagg atacatttt aacgttcct ggtatcggc ggcttttct cctaatagg cgaattgaa tgcaatcgc accgttgat ttccaattt ttatctagc ctcgtacgc ccttatcaa tttttgcac tttccatgc cgcgctaat caaaccctt ggggtggag accgacgtt caaatatta caatacgtg gctaagcct tgccgggtt catcagctg cgtcgttat catgttctt aattaattg gcaatcatc cctaattac tttcggcgt aattatcgc cttgtatct cttggtggt ttaccgaaa gccagatgt taattgggg ctcgtccac tcaggccca gacgtacaa aacggccct gacaacggc ccaccgctt gataaacat cggggagca cgtaaaaaa ttacaaacc gcaaatttt ggtgctttg tggctcccc gtcctcacg gcccgctct aatcgattt tggcaactt gataacgaa aaacaattc gttgctgat catcgggga cgtgacaga ggcgaggct gcagcaccg ggccaggtc ggctcgcat ccaaaacgt ctactcctg gctcaggat taacaaaat caaagcctt ttactcgat tttggacaa cacgcagtt cgaaaacgg ggccaaggc tttgctgca aacggccgg catcggcaa gctttgccc caacgtcct catgatggg ttataagaa cgactggag gacttgtcc ccgtgagga attaatggc ttattttaa tgttttaca tgcggcgtt tgaccaccg ccttgctgt gacaatttt gcgtctgaa agacggtct ggattacag taaactgat gggcgaaga tgatggttc gcccagcga gggcactct gtcccagca acccacaat ggatttgcg aggtttcgc cacaagtag tatgatgca tgctgattc gaatttaag tttagctga cttaggaaa atcagatac tgttacgtc taaaaatac aataaacgg ggtcaaaaa cggtcacgg gttgttaaa ttcagtctt tgccaagaa acagaaagt cgctgattc gacccccga caagaccag tgtgcaaca tatccccaa tttcgcaat ggagaatat gactctgtt ccgaaataa gcaaaacaa gggaaacat cgatgtttg gtggttgta tgacgatgg gggcctgac gatgctttt gccgtacat tatttcaac caggcagca ttgggccaa ttgcaagct gcgagtttt cgccctcag tacaaacaa aaacgagat cgaagaaga ggagaaaaa tatggcgtt tttgttgtc gaaattccg actgaatta ctccagttt gaaaatcgt ttcgctttc ggataagcc acaagaggc gatgattaa atttttcaa cgatatgat tagcgattt taggacgaa tgaggacct cgatgatga atccaaggt ttccgaagc cgagattgc ctcgcttca ggataagac gtaccggca gttgagact tcgtgagtt gctgttaga gcactcgag tgaagcaaa tttgattgt catgtcttt accgatgcc gaggaaagg caaattatc agcgccttt gtacatggc atggttgga agcgctgac ccgggattt gcccccgta tttgctagt gagagggaa ccagcagtc cgttctcac tttttattc ctaaggaac gtactcaag aagtattct tataccaaa aatgttatt gatagcgtt aagtgtagt ttacgtgcc taaatattt attttatta actgtaata attatgtaa tcaaatttt ataaatttg tacgacgac aaagacttt aataaatat tttttgtag aa
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