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Gene information
General information
TC000570 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 4125738..4130543
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000570
    iBeetle screen iB_00129
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 100
    Pupal morphology
    elytra distal orientation irregular - Number of animals showing the phenotype: 2

    Adult morphology
    pronotum shape irregular - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    remarks: later than schedule, hours 100
    Fertility
    egg production: number no/almost no eggs -
    tropharium partially size decreased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4
    egg chamber sometimes segmentation/division not present - Number of animals showing the phenotype: 4
    Comment: May be in some cases due to preparation artifacts
    egg chamber differentiation irregular - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000570 RA
      MSHLSRVFL HSRDLPRFL GHFRARRSF HACVTTNLT 
TLSHTALDP VSKVFPLDL PKKSFSKLP RKTFNGAAT 
GSVRFFSSK DDSDGEKPP EEEPYNTQL PATVAVPEV 
WPHVPVIAI SRNIVFPRF IKLIELTNP QLIELIRRK 
VKLNQPYCG IFLKKNEEN DAEVVNNIN DVYNVGVFA 
QIHEMQDLG DRLRLVVMA HRRIKITGQ IIDNDQDGI 
KEEADAEKR RRKPARNNR VKKTETKTP DDKKEPPKE 
QFLMAEVEN VMHNKFKQT EEVKALTQE VIKTIRDII 
SLNPLYRDS LQQMMHQGQ RVVDNPVYL SDLGAALTA 
AEAKELQEV LEEMDIPKR LMLSLSLLK KEYELSKLQ 
QKIGKEVEE KVKQHHRKY ILQEQLKVI KKELGLEKE 
DKDAVGDKF RERIKDKVL PEAVNSVIE EELNKLNFL 
ESHSSEFNV TRNYLDWLT SLPWGVYSE ENLNLQRAS 
EILDQDHYG MEDIKRRIL EFIAVSQLK GSTQGKILC 
FHGPPGVGK TSIARSIAR ALNREYFRF SVGGMTDVA 
EIKGHRRTY VGAMPGKVI QCLKKTRTE NPLILIDEV 
DKIGKGYTG DPSSALLEL LDPEQNANF LDHYLDVPV 
DLSKVLFIC TANVVETIP EPLRDRMEM IDMSGYVAE 
EKLAIATKY LLPQAMKDS GLNDQHIKI DDEALNTLI 
KSYCRESGV RNLQKHIEK VVRKVAYKV VKEETNFVE 
VSNANLAEF VGKPVFSQE RMYPTTPPG VVMGLAWTA 
MGGSTLYIE TTTRKIAAE KETDGSLEL TGHLGDVMK 
ESAKIALTV ARNYLYKID STNKFLQSS HLHLHVPEG 
ATPKDGPSA GCTIVTALL SLAKNEPIR QDVAMTGEI 
SLTGKVLPV GGIKEKTIA AKRSGVKCI ILPEENKKD 
FNDLPKFIT DGLEVHFVS TYDEVYNIV FNKNL
    CDS sequence
    • >TC000570 RA
      atgtcgcat ttatcgcgt gttttcctc cactcgcgt 
gatttgcct cgtttttta ggacatttc cgcgcgcga 
agaagtttt cacgcgtgt gtaacaaca aacttaacc 
acactttcg cacaccgct ttagacccc gtgtccaaa 
gtctttcct ttggattta cccaagaag agcttttcg 
aaattgcct cgaaagacc tttaatggg gcggcgacc 
ggaagtgtg cgttttttc agttcgaag gacgactca 
gatggggaa aagcccccc gaggaggag ccctacaac 
acccagttg ccggccact gtggccgtc cccgaggtc 
tggccccac gtgcctgtc attgccatt agtcgcaat 
atagttttt ccacgtttt attaaacta atcgagctc 
actaatccg caattaatc gagttgata aggcgcaaa 
gttaagtta aatcaaccg tattgtggg attttcctc 
aagaaaaat gaggaaaac gatgctgaa gttgttaac 
aacattaat gatgtttac aatgttggg gttttcgcc 
caaattcat gaaatgcag gacttgggg gatcgtttg 
agactcgta gttatggcc catcgtaga attaaaatc 
acggggcaa atcatagac aacgaccag gacggaatt 
aaagaggaa gctgacgcg gaaaaacgg cgcagaaaa 
cccgcccga aataaccgc gttaaaaaa acagaaacc 
aaaacacct gacgataaa aaagagccc ccgaaagag 
cagttctta atggccgaa gtcgagaac gtcatgcac 
aacaaattc aaacaaacc gaggaggtc aaagctttg 
actcaggaa gttattaaa acaattcgt gatattatt 
agtttaaat cctctgtat cgggacagt ttgcaacaa 
atgatgcat caggggcag agagtggtc gataatccg 
gtttatttg agcgacttg ggggccgct ctcacggcc 
gcggaagcg aaggaatta caggaagtt ttagaggag 
atggatatt ccaaaacgc ctaatgttg tcactctca 
ctgctcaaa aaagagtac gagctgtcg aagttgcaa 
caaaaaatc gggaaagaa gtcgaggaa aaggtgaag 
caacaccac cgaaagtac atccttcag gaacaactc 
aaagtgatt aagaaagaa ttgggcttg gagaaggag 
gataaagac gcggtaggt gacaaattc cgggagaga 
atcaaagat aaagtcttg ccggaagcc gtcaactcc 
gtaattgag gaagaatta aacaaactc aatttcttg 
gaaagtcac agttcggag tttaatgtc acgcggaac 
tacctcgac tggttgacg tcactgcct tggggggtt 
tacagcgag gagaattta aatttgcaa agagcgtcg 
gaaattcta gatcaggat cattacgga atggaggat 
attaaacga cgcattttg gaatttatc gccgttagt 
cagttaaaa ggttcgact caagggaaa attttgtgt 
ttccatgga cctcccggt gtcggaaaa actagcatc 
gccaggtcg atcgcgcgt gccttgaac cgggagtat 
tttaggttt tcggtgggt ggtatgacc gatgtggcc 
gaaattaag ggccatagg aggacctac gtgggggcc 
atgcccgga aaagtcatc cagtgtttg aagaaaacg 
cgcaccgaa aatccccta attctcatt gatgaggtc 
gacaagatc ggcaaggga tacaccggc gacccgagt 
tcggccctc ctcgaactt ctggacccg gaacaaaat 
gcgaatttc ctcgaccat tacctcgat gttccggtt 
gatttatcc aaagtattg ttcatttgt actgcgaac 
gttgtcgag accattccc gagcctttg cgcgaccgt 
atggaaatg atagatatg tcgggttac gtagcagag 
gagaaactc gcaattgcg accaaatac ctcctgcca 
caagcaatg aaagatagc ggtttgaac gaccaacat 
atcaaaatc gacgacgag gccctaaac accctcatc 
aagagttac tgtcgcgaa tcaggcgtt cgaaacttg 
cagaaacac atcgagaaa gtggtccga aaagttgcc 
tacaaagtc gtgaaagaa gaaacgaac tttgtcgag 
gtctccaat gcgaatttg gccgagttt gtgggcaaa 
ccggtgttt tcgcaagag aggatgtac cccacgacg 
cccccgggg gtcgtcatg gggctggcg tggactgct 
atggggggg tcaactctc tacattgag accacaacg 
cgcaaaatt gcagcagag aaggagaca gacgggtct 
ttggaatta acggggcat ttgggggac gttatgaaa 
gaatcggcc aaaatcgcc ctgactgtg gccaggaat 
tatctctac aaaatagac agcactaat aaatttttg 
caaagtagt catttacac ttgcatgtg ccggaaggc 
gcaacgccg aaagatggc cctagtgcg ggttgtaca 
attgtcact gctttgtta tcgctggct aagaatgaa 
ccaatcagg caggatgta gcaatgacg ggggagatt 
tcactgacg gggaaggtt ttaccggtc ggagggatt 
aaagaaaag acaattgcg gccaaaaga tctggagtc 
aagtgtata atactgcca gaggaaaac aagaaagat 
ttcaatgat cttccgaaa tttatcacc gacgggttg 
gaagtacat ttcgttagt acttacgac gaggtttat 
aatattgtt tttaataaa aatctttga
    mRNA sequence
    • >TC000570 RA
      tctcgactt ttccatcag tgttaaaat ttgaaatgt 
ttttgtgat tttatgaaa aaatgtcgc atttatcgc 
gtgttttcc tccactcgc gtgatttgc ctcgttttt 
taggacatt tccgcgcgc gaagaagtt ttcacgcgt 
gtgtaacaa caaacttaa ccacacttt cgcacaccg 
ctttagacc ccgtgtcca aagtctttc ctttggatt 
tacccaaga agagctttt cgaaattgc ctcgaaaga 
cctttaatg gggcggcga ccggaagtg tgcgttttt 
tcagttcga aggacgact cagatgggg aaaagcccc 
ccgaggagg agccctaca acacccagt tgccggcca 
ctgtggccg tccccgagg tctggcccc acgtgcctg 
tcattgcca ttagtcgca atatagttt ttccacgtt 
ttattaaac taatcgagc tcactaatc cgcaattaa 
tcgagttga taaggcgca aagttaagt taaatcaac 
cgtattgtg ggattttcc tcaagaaaa atgaggaaa 
acgatgctg aagttgtta acaacatta atgatgttt 
acaatgttg gggttttcg cccaaattc atgaaatgc 
aggacttgg gggatcgtt tgagactcg tagttatgg 
cccatcgta gaattaaaa tcacggggc aaatcatag 
acaacgacc aggacggaa ttaaagagg aagctgacg 
cggaaaaac ggcgcagaa aacccgccc gaaataacc 
gcgttaaaa aaacagaaa ccaaaacac ctgacgata 
aaaaagagc ccccgaaag agcagttct taatggccg 
aagtcgaga acgtcatgc acaacaaat tcaaacaaa 
ccgaggagg tcaaagctt tgactcagg aagttatta 
aaacaattc gtgatatta ttagtttaa atcctctgt 
atcgggaca gtttgcaac aaatgatgc atcaggggc 
agagagtgg tcgataatc cggtttatt tgagcgact 
tgggggccg ctctcacgg ccgcggaag cgaaggaat 
tacaggaag ttttagagg agatggata ttccaaaac 
gcctaatgt tgtcactct cactgctca aaaaagagt 
acgagctgt cgaagttgc aacaaaaaa tcgggaaag 
aagtcgagg aaaaggtga agcaacacc accgaaagt 
acatccttc aggaacaac tcaaagtga ttaagaaag 
aattgggct tggagaagg aggataaag acgcggtag 
gtgacaaat tccgggaga gaatcaaag ataaagtct 
tgccggaag ccgtcaact ccgtaattg aggaagaat 
taaacaaac tcaatttct tggaaagtc acagttcgg 
agtttaatg tcacgcgga actacctcg actggttga 
cgtcactgc cttgggggg tttacagcg aggagaatt 
taaatttgc aaagagcgt cggaaattc tagatcagg 
atcattacg gaatggagg atattaaac gacgcattt 
tggaattta tcgccgtta gtcagttaa aaggttcga 
ctcaaggga aaattttgt gtttccatg gacctcccg 
gtgtcggaa aaactagca tcgccaggt cgatcgcgc 
gtgccttga accgggagt attttaggt tttcggtgg 
gtggtatga ccgatgtgg ccgaaatta agggccata 
ggaggacct acgtggggg ccatgcccg gaaaagtca 
tccagtgtt tgaagaaaa cgcgcaccg aaaatcccc 
taattctca ttgatgagg tcgacaaga tcggcaagg 
gatacaccg gcgacccga gttcggccc tcctcgaac 
ttctggacc cggaacaaa atgcgaatt tcctcgacc 
attacctcg atgttccgg ttgatttat ccaaagtat 
tgttcattt gtactgcga acgttgtcg agaccattc 
ccgagcctt tgcgcgacc gtatggaaa tgatagata 
tgtcgggtt acgtagcag aggagaaac tcgcaattg 
cgaccaaat acctcctgc cacaagcaa tgaaagata 
gcggtttga acgaccaac atatcaaaa tcgacgacg 
aggccctaa acaccctca tcaagagtt actgtcgcg 
aatcaggcg ttcgaaact tgcagaaac acatcgaga 
aagtggtcc gaaaagttg cctacaaag tcgtgaaag 
aagaaacga actttgtcg aggtctcca atgcgaatt 
tggccgagt ttgtgggca aaccggtgt tttcgcaag 
agaggatgt accccacga cgcccccgg gggtcgtca 
tggggctgg cgtggactg ctatggggg ggtcaactc 
tctacattg agaccacaa cgcgcaaaa ttgcagcag 
agaaggaga cagacgggt ctttggaat taacggggc 
atttggggg acgttatga aagaatcgg ccaaaatcg 
ccctgactg tggccagga attatctct acaaaatag 
acagcacta ataaatttt tgcaaagta gtcatttac 
acttgcatg tgccggaag gcgcaacgc cgaaagatg 
gccctagtg cgggttgta caattgtca ctgctttgt 
tatcgctgg ctaagaatg aaccaatca ggcaggatg 
tagcaatga cgggggaga tttcactga cggggaagg 
ttttaccgg tcggaggga ttaaagaaa agacaattg 
cggccaaaa gatctggag tcaagtgta taatactgc 
cagaggaaa acaagaaag atttcaatg atcttccga 
aatttatca ccgacgggt tggaagtac atttcgtta 
gtacttacg acgaggttt ataatattg tttttaata 
aaaatcttt gattgtgat agtgttcac ttttttata 
cagacaagt gttttaatt gatttgtat aaaaatcgt 
atttattgt acaaaatag tcgaataaa gtttggatt 
atttg