General information | |||
TC000570 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 9632281..9637086 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC000570 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00129 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
remarks: later than schedule, hours 100
tropharium partially size decreased - Number of animals showing the phenotype: 4
vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4
egg chamber sometimes segmentation/division not present - Number of animals showing the phenotype: 4
Comment: May be in some cases due to preparation artifacts |
- >TC000570 RA
MSHLSRVFL HSRDLPRFL GHFRARRSF HACVTTNLT TLSHTALDP VSKVFPLDL PKKSFSKLP RKTFNGAAT GSVRFFSSK DDSDGEKPP EEEPYNTQL PATVAVPEV WPHVPVIAI SRNIVFPRF IKLIELTNP QLIELIRRK VKLNQPYCG IFLKKNEEN DAEVVNNIN DVYNVGVFA QIHEMQDLG DRLRLVVMA HRRIKITGQ IIDNDQDGI KEEADAEKR RRKPARNNR VKKTETKTP DDKKEPPKE QFLMAEVEN VMHNKFKQT EEVKALTQE VIKTIRDII SLNPLYRDS LQQMMHQGQ RVVDNPVYL SDLGAALTA AEAKELQEV LEEMDIPKR LMLSLSLLK KEYELSKLQ QKIGKEVEE KVKQHHRKY ILQEQLKVI KKELGLEKE DKDAVGDKF RERIKDKVL PEAVNSVIE EELNKLNFL ESHSSEFNV TRNYLDWLT SLPWGVYSE ENLNLQRAS EILDQDHYG MEDIKRRIL EFIAVSQLK GSTQGKILC FHGPPGVGK TSIARSIAR ALNREYFRF SVGGMTDVA EIKGHRRTY VGAMPGKVI QCLKKTRTE NPLILIDEV DKIGKGYTG DPSSALLEL LDPEQNANF LDHYLDVPV DLSKVLFIC TANVVETIP EPLRDRMEM IDMSGYVAE EKLAIATKY LLPQAMKDS GLNDQHIKI DDEALNTLI KSYCRESGV RNLQKHIEK VVRKVAYKV VKEETNFVE VSNANLAEF VGKPVFSQE RMYPTTPPG VVMGLAWTA MGGSTLYIE TTTRKIAAE KETDGSLEL TGHLGDVMK ESAKIALTV ARNYLYKID STNKFLQSS HLHLHVPEG ATPKDGPSA GCTIVTALL SLAKNEPIR QDVAMTGEI SLTGKVLPV GGIKEKTIA AKRSGVKCI ILPEENKKD FNDLPKFIT DGLEVHFVS TYDEVYNIV FNKNL
- >TC000570 RA
atgtcgcat ttatcgcgt gttttcctc cactcgcgt gatttgcct cgtttttta ggacatttc cgcgcgcga agaagtttt cacgcgtgt gtaacaaca aacttaacc acactttcg cacaccgct ttagacccc gtgtccaaa gtctttcct ttggattta cccaagaag agcttttcg aaattgcct cgaaagacc tttaatggg gcggcgacc ggaagtgtg cgttttttc agttcgaag gacgactca gatggggaa aagcccccc gaggaggag ccctacaac acccagttg ccggccact gtggccgtc cccgaggtc tggccccac gtgcctgtc attgccatt agtcgcaat atagttttt ccacgtttt attaaacta atcgagctc actaatccg caattaatc gagttgata aggcgcaaa gttaagtta aatcaaccg tattgtggg attttcctc aagaaaaat gaggaaaac gatgctgaa gttgttaac aacattaat gatgtttac aatgttggg gttttcgcc caaattcat gaaatgcag gacttgggg gatcgtttg agactcgta gttatggcc catcgtaga attaaaatc acggggcaa atcatagac aacgaccag gacggaatt aaagaggaa gctgacgcg gaaaaacgg cgcagaaaa cccgcccga aataaccgc gttaaaaaa acagaaacc aaaacacct gacgataaa aaagagccc ccgaaagag cagttctta atggccgaa gtcgagaac gtcatgcac aacaaattc aaacaaacc gaggaggtc aaagctttg actcaggaa gttattaaa acaattcgt gatattatt agtttaaat cctctgtat cgggacagt ttgcaacaa atgatgcat caggggcag agagtggtc gataatccg gtttatttg agcgacttg ggggccgct ctcacggcc gcggaagcg aaggaatta caggaagtt ttagaggag atggatatt ccaaaacgc ctaatgttg tcactctca ctgctcaaa aaagagtac gagctgtcg aagttgcaa caaaaaatc gggaaagaa gtcgaggaa aaggtgaag caacaccac cgaaagtac atccttcag gaacaactc aaagtgatt aagaaagaa ttgggcttg gagaaggag gataaagac gcggtaggt gacaaattc cgggagaga atcaaagat aaagtcttg ccggaagcc gtcaactcc gtaattgag gaagaatta aacaaactc aatttcttg gaaagtcac agttcggag tttaatgtc acgcggaac tacctcgac tggttgacg tcactgcct tggggggtt tacagcgag gagaattta aatttgcaa agagcgtcg gaaattcta gatcaggat cattacgga atggaggat attaaacga cgcattttg gaatttatc gccgttagt cagttaaaa ggttcgact caagggaaa attttgtgt ttccatgga cctcccggt gtcggaaaa actagcatc gccaggtcg atcgcgcgt gccttgaac cgggagtat tttaggttt tcggtgggt ggtatgacc gatgtggcc gaaattaag ggccatagg aggacctac gtgggggcc atgcccgga aaagtcatc cagtgtttg aagaaaacg cgcaccgaa aatccccta attctcatt gatgaggtc gacaagatc ggcaaggga tacaccggc gacccgagt tcggccctc ctcgaactt ctggacccg gaacaaaat gcgaatttc ctcgaccat tacctcgat gttccggtt gatttatcc aaagtattg ttcatttgt actgcgaac gttgtcgag accattccc gagcctttg cgcgaccgt atggaaatg atagatatg tcgggttac gtagcagag gagaaactc gcaattgcg accaaatac ctcctgcca caagcaatg aaagatagc ggtttgaac gaccaacat atcaaaatc gacgacgag gccctaaac accctcatc aagagttac tgtcgcgaa tcaggcgtt cgaaacttg cagaaacac atcgagaaa gtggtccga aaagttgcc tacaaagtc gtgaaagaa gaaacgaac tttgtcgag gtctccaat gcgaatttg gccgagttt gtgggcaaa ccggtgttt tcgcaagag aggatgtac cccacgacg cccccgggg gtcgtcatg gggctggcg tggactgct atggggggg tcaactctc tacattgag accacaacg cgcaaaatt gcagcagag aaggagaca gacgggtct ttggaatta acggggcat ttgggggac gttatgaaa gaatcggcc aaaatcgcc ctgactgtg gccaggaat tatctctac aaaatagac agcactaat aaatttttg caaagtagt catttacac ttgcatgtg ccggaaggc gcaacgccg aaagatggc cctagtgcg ggttgtaca attgtcact gctttgtta tcgctggct aagaatgaa ccaatcagg caggatgta gcaatgacg ggggagatt tcactgacg gggaaggtt ttaccggtc ggagggatt aaagaaaag acaattgcg gccaaaaga tctggagtc aagtgtata atactgcca gaggaaaac aagaaagat ttcaatgat cttccgaaa tttatcacc gacgggttg gaagtacat ttcgttagt acttacgac gaggtttat aatattgtt tttaataaa aatctttga
- >TC000570 RA
tctcgactt ttccatcag tgttaaaat ttgaaatgt ttttgtgat tttatgaaa aaatgtcgc atttatcgc gtgttttcc tccactcgc gtgatttgc ctcgttttt taggacatt tccgcgcgc gaagaagtt ttcacgcgt gtgtaacaa caaacttaa ccacacttt cgcacaccg ctttagacc ccgtgtcca aagtctttc ctttggatt tacccaaga agagctttt cgaaattgc ctcgaaaga cctttaatg gggcggcga ccggaagtg tgcgttttt tcagttcga aggacgact cagatgggg aaaagcccc ccgaggagg agccctaca acacccagt tgccggcca ctgtggccg tccccgagg tctggcccc acgtgcctg tcattgcca ttagtcgca atatagttt ttccacgtt ttattaaac taatcgagc tcactaatc cgcaattaa tcgagttga taaggcgca aagttaagt taaatcaac cgtattgtg ggattttcc tcaagaaaa atgaggaaa acgatgctg aagttgtta acaacatta atgatgttt acaatgttg gggttttcg cccaaattc atgaaatgc aggacttgg gggatcgtt tgagactcg tagttatgg cccatcgta gaattaaaa tcacggggc aaatcatag acaacgacc aggacggaa ttaaagagg aagctgacg cggaaaaac ggcgcagaa aacccgccc gaaataacc gcgttaaaa aaacagaaa ccaaaacac ctgacgata aaaaagagc ccccgaaag agcagttct taatggccg aagtcgaga acgtcatgc acaacaaat tcaaacaaa ccgaggagg tcaaagctt tgactcagg aagttatta aaacaattc gtgatatta ttagtttaa atcctctgt atcgggaca gtttgcaac aaatgatgc atcaggggc agagagtgg tcgataatc cggtttatt tgagcgact tgggggccg ctctcacgg ccgcggaag cgaaggaat tacaggaag ttttagagg agatggata ttccaaaac gcctaatgt tgtcactct cactgctca aaaaagagt acgagctgt cgaagttgc aacaaaaaa tcgggaaag aagtcgagg aaaaggtga agcaacacc accgaaagt acatccttc aggaacaac tcaaagtga ttaagaaag aattgggct tggagaagg aggataaag acgcggtag gtgacaaat tccgggaga gaatcaaag ataaagtct tgccggaag ccgtcaact ccgtaattg aggaagaat taaacaaac tcaatttct tggaaagtc acagttcgg agtttaatg tcacgcgga actacctcg actggttga cgtcactgc cttgggggg tttacagcg aggagaatt taaatttgc aaagagcgt cggaaattc tagatcagg atcattacg gaatggagg atattaaac gacgcattt tggaattta tcgccgtta gtcagttaa aaggttcga ctcaaggga aaattttgt gtttccatg gacctcccg gtgtcggaa aaactagca tcgccaggt cgatcgcgc gtgccttga accgggagt attttaggt tttcggtgg gtggtatga ccgatgtgg ccgaaatta agggccata ggaggacct acgtggggg ccatgcccg gaaaagtca tccagtgtt tgaagaaaa cgcgcaccg aaaatcccc taattctca ttgatgagg tcgacaaga tcggcaagg gatacaccg gcgacccga gttcggccc tcctcgaac ttctggacc cggaacaaa atgcgaatt tcctcgacc attacctcg atgttccgg ttgatttat ccaaagtat tgttcattt gtactgcga acgttgtcg agaccattc ccgagcctt tgcgcgacc gtatggaaa tgatagata tgtcgggtt acgtagcag aggagaaac tcgcaattg cgaccaaat acctcctgc cacaagcaa tgaaagata gcggtttga acgaccaac atatcaaaa tcgacgacg aggccctaa acaccctca tcaagagtt actgtcgcg aatcaggcg ttcgaaact tgcagaaac acatcgaga aagtggtcc gaaaagttg cctacaaag tcgtgaaag aagaaacga actttgtcg aggtctcca atgcgaatt tggccgagt ttgtgggca aaccggtgt tttcgcaag agaggatgt accccacga cgcccccgg gggtcgtca tggggctgg cgtggactg ctatggggg ggtcaactc tctacattg agaccacaa cgcgcaaaa ttgcagcag agaaggaga cagacgggt ctttggaat taacggggc atttggggg acgttatga aagaatcgg ccaaaatcg ccctgactg tggccagga attatctct acaaaatag acagcacta ataaatttt tgcaaagta gtcatttac acttgcatg tgccggaag gcgcaacgc cgaaagatg gccctagtg cgggttgta caattgtca ctgctttgt tatcgctgg ctaagaatg aaccaatca ggcaggatg tagcaatga cgggggaga tttcactga cggggaagg ttttaccgg tcggaggga ttaaagaaa agacaattg cggccaaaa gatctggag tcaagtgta taatactgc cagaggaaa acaagaaag atttcaatg atcttccga aatttatca ccgacgggt tggaagtac atttcgtta gtacttacg acgaggttt ataatattg tttttaata aaaatcttt gattgtgat agtgttcac ttttttata cagacaagt gttttaatt gatttgtat aaaaatcgt atttattgt acaaaatag tcgaataaa gtttggatt atttg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
