Status
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Gene information
General information
TC000570 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9632281..9637086
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000570
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00129

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 100
    Pupal morphology
    elytra distal orientation irregular - Number of animals showing the phenotype: 2

    Adult morphology
    pronotum shape irregular - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection



    remarks: later than schedule, hours 100
    Fertility
    egg production: number no/almost no eggs -
    tropharium partially size decreased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4
    egg chamber sometimes segmentation/division not present - Number of animals showing the phenotype: 4
    Comment: May be in some cases due to preparation artifacts
    egg chamber differentiation irregular - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000570 RA
      MSHLSRVFL HSRDLPRFL GHFRARRSF HACVTTNLT 
      TLSHTALDP VSKVFPLDL PKKSFSKLP RKTFNGAAT 
      GSVRFFSSK DDSDGEKPP EEEPYNTQL PATVAVPEV 
      WPHVPVIAI SRNIVFPRF IKLIELTNP QLIELIRRK 
      VKLNQPYCG IFLKKNEEN DAEVVNNIN DVYNVGVFA 
      QIHEMQDLG DRLRLVVMA HRRIKITGQ IIDNDQDGI 
      KEEADAEKR RRKPARNNR VKKTETKTP DDKKEPPKE 
      QFLMAEVEN VMHNKFKQT EEVKALTQE VIKTIRDII 
      SLNPLYRDS LQQMMHQGQ RVVDNPVYL SDLGAALTA 
      AEAKELQEV LEEMDIPKR LMLSLSLLK KEYELSKLQ 
      QKIGKEVEE KVKQHHRKY ILQEQLKVI KKELGLEKE 
      DKDAVGDKF RERIKDKVL PEAVNSVIE EELNKLNFL 
      ESHSSEFNV TRNYLDWLT SLPWGVYSE ENLNLQRAS 
      EILDQDHYG MEDIKRRIL EFIAVSQLK GSTQGKILC 
      FHGPPGVGK TSIARSIAR ALNREYFRF SVGGMTDVA 
      EIKGHRRTY VGAMPGKVI QCLKKTRTE NPLILIDEV 
      DKIGKGYTG DPSSALLEL LDPEQNANF LDHYLDVPV 
      DLSKVLFIC TANVVETIP EPLRDRMEM IDMSGYVAE 
      EKLAIATKY LLPQAMKDS GLNDQHIKI DDEALNTLI 
      KSYCRESGV RNLQKHIEK VVRKVAYKV VKEETNFVE 
      VSNANLAEF VGKPVFSQE RMYPTTPPG VVMGLAWTA 
      MGGSTLYIE TTTRKIAAE KETDGSLEL TGHLGDVMK 
      ESAKIALTV ARNYLYKID STNKFLQSS HLHLHVPEG 
      ATPKDGPSA GCTIVTALL SLAKNEPIR QDVAMTGEI 
      SLTGKVLPV GGIKEKTIA AKRSGVKCI ILPEENKKD 
      FNDLPKFIT DGLEVHFVS TYDEVYNIV FNKNL
    CDS sequence
    • >TC000570 RA
      atgtcgcat ttatcgcgt gttttcctc cactcgcgt 
      gatttgcct cgtttttta ggacatttc cgcgcgcga 
      agaagtttt cacgcgtgt gtaacaaca aacttaacc 
      acactttcg cacaccgct ttagacccc gtgtccaaa 
      gtctttcct ttggattta cccaagaag agcttttcg 
      aaattgcct cgaaagacc tttaatggg gcggcgacc 
      ggaagtgtg cgttttttc agttcgaag gacgactca 
      gatggggaa aagcccccc gaggaggag ccctacaac 
      acccagttg ccggccact gtggccgtc cccgaggtc 
      tggccccac gtgcctgtc attgccatt agtcgcaat 
      atagttttt ccacgtttt attaaacta atcgagctc 
      actaatccg caattaatc gagttgata aggcgcaaa 
      gttaagtta aatcaaccg tattgtggg attttcctc 
      aagaaaaat gaggaaaac gatgctgaa gttgttaac 
      aacattaat gatgtttac aatgttggg gttttcgcc 
      caaattcat gaaatgcag gacttgggg gatcgtttg 
      agactcgta gttatggcc catcgtaga attaaaatc 
      acggggcaa atcatagac aacgaccag gacggaatt 
      aaagaggaa gctgacgcg gaaaaacgg cgcagaaaa 
      cccgcccga aataaccgc gttaaaaaa acagaaacc 
      aaaacacct gacgataaa aaagagccc ccgaaagag 
      cagttctta atggccgaa gtcgagaac gtcatgcac 
      aacaaattc aaacaaacc gaggaggtc aaagctttg 
      actcaggaa gttattaaa acaattcgt gatattatt 
      agtttaaat cctctgtat cgggacagt ttgcaacaa 
      atgatgcat caggggcag agagtggtc gataatccg 
      gtttatttg agcgacttg ggggccgct ctcacggcc 
      gcggaagcg aaggaatta caggaagtt ttagaggag 
      atggatatt ccaaaacgc ctaatgttg tcactctca 
      ctgctcaaa aaagagtac gagctgtcg aagttgcaa 
      caaaaaatc gggaaagaa gtcgaggaa aaggtgaag 
      caacaccac cgaaagtac atccttcag gaacaactc 
      aaagtgatt aagaaagaa ttgggcttg gagaaggag 
      gataaagac gcggtaggt gacaaattc cgggagaga 
      atcaaagat aaagtcttg ccggaagcc gtcaactcc 
      gtaattgag gaagaatta aacaaactc aatttcttg 
      gaaagtcac agttcggag tttaatgtc acgcggaac 
      tacctcgac tggttgacg tcactgcct tggggggtt 
      tacagcgag gagaattta aatttgcaa agagcgtcg 
      gaaattcta gatcaggat cattacgga atggaggat 
      attaaacga cgcattttg gaatttatc gccgttagt 
      cagttaaaa ggttcgact caagggaaa attttgtgt 
      ttccatgga cctcccggt gtcggaaaa actagcatc 
      gccaggtcg atcgcgcgt gccttgaac cgggagtat 
      tttaggttt tcggtgggt ggtatgacc gatgtggcc 
      gaaattaag ggccatagg aggacctac gtgggggcc 
      atgcccgga aaagtcatc cagtgtttg aagaaaacg 
      cgcaccgaa aatccccta attctcatt gatgaggtc 
      gacaagatc ggcaaggga tacaccggc gacccgagt 
      tcggccctc ctcgaactt ctggacccg gaacaaaat 
      gcgaatttc ctcgaccat tacctcgat gttccggtt 
      gatttatcc aaagtattg ttcatttgt actgcgaac 
      gttgtcgag accattccc gagcctttg cgcgaccgt 
      atggaaatg atagatatg tcgggttac gtagcagag 
      gagaaactc gcaattgcg accaaatac ctcctgcca 
      caagcaatg aaagatagc ggtttgaac gaccaacat 
      atcaaaatc gacgacgag gccctaaac accctcatc 
      aagagttac tgtcgcgaa tcaggcgtt cgaaacttg 
      cagaaacac atcgagaaa gtggtccga aaagttgcc 
      tacaaagtc gtgaaagaa gaaacgaac tttgtcgag 
      gtctccaat gcgaatttg gccgagttt gtgggcaaa 
      ccggtgttt tcgcaagag aggatgtac cccacgacg 
      cccccgggg gtcgtcatg gggctggcg tggactgct 
      atggggggg tcaactctc tacattgag accacaacg 
      cgcaaaatt gcagcagag aaggagaca gacgggtct 
      ttggaatta acggggcat ttgggggac gttatgaaa 
      gaatcggcc aaaatcgcc ctgactgtg gccaggaat 
      tatctctac aaaatagac agcactaat aaatttttg 
      caaagtagt catttacac ttgcatgtg ccggaaggc 
      gcaacgccg aaagatggc cctagtgcg ggttgtaca 
      attgtcact gctttgtta tcgctggct aagaatgaa 
      ccaatcagg caggatgta gcaatgacg ggggagatt 
      tcactgacg gggaaggtt ttaccggtc ggagggatt 
      aaagaaaag acaattgcg gccaaaaga tctggagtc 
      aagtgtata atactgcca gaggaaaac aagaaagat 
      ttcaatgat cttccgaaa tttatcacc gacgggttg 
      gaagtacat ttcgttagt acttacgac gaggtttat 
      aatattgtt tttaataaa aatctttga
    mRNA sequence
    • >TC000570 RA
      tctcgactt ttccatcag tgttaaaat ttgaaatgt 
      ttttgtgat tttatgaaa aaatgtcgc atttatcgc 
      gtgttttcc tccactcgc gtgatttgc ctcgttttt 
      taggacatt tccgcgcgc gaagaagtt ttcacgcgt 
      gtgtaacaa caaacttaa ccacacttt cgcacaccg 
      ctttagacc ccgtgtcca aagtctttc ctttggatt 
      tacccaaga agagctttt cgaaattgc ctcgaaaga 
      cctttaatg gggcggcga ccggaagtg tgcgttttt 
      tcagttcga aggacgact cagatgggg aaaagcccc 
      ccgaggagg agccctaca acacccagt tgccggcca 
      ctgtggccg tccccgagg tctggcccc acgtgcctg 
      tcattgcca ttagtcgca atatagttt ttccacgtt 
      ttattaaac taatcgagc tcactaatc cgcaattaa 
      tcgagttga taaggcgca aagttaagt taaatcaac 
      cgtattgtg ggattttcc tcaagaaaa atgaggaaa 
      acgatgctg aagttgtta acaacatta atgatgttt 
      acaatgttg gggttttcg cccaaattc atgaaatgc 
      aggacttgg gggatcgtt tgagactcg tagttatgg 
      cccatcgta gaattaaaa tcacggggc aaatcatag 
      acaacgacc aggacggaa ttaaagagg aagctgacg 
      cggaaaaac ggcgcagaa aacccgccc gaaataacc 
      gcgttaaaa aaacagaaa ccaaaacac ctgacgata 
      aaaaagagc ccccgaaag agcagttct taatggccg 
      aagtcgaga acgtcatgc acaacaaat tcaaacaaa 
      ccgaggagg tcaaagctt tgactcagg aagttatta 
      aaacaattc gtgatatta ttagtttaa atcctctgt 
      atcgggaca gtttgcaac aaatgatgc atcaggggc 
      agagagtgg tcgataatc cggtttatt tgagcgact 
      tgggggccg ctctcacgg ccgcggaag cgaaggaat 
      tacaggaag ttttagagg agatggata ttccaaaac 
      gcctaatgt tgtcactct cactgctca aaaaagagt 
      acgagctgt cgaagttgc aacaaaaaa tcgggaaag 
      aagtcgagg aaaaggtga agcaacacc accgaaagt 
      acatccttc aggaacaac tcaaagtga ttaagaaag 
      aattgggct tggagaagg aggataaag acgcggtag 
      gtgacaaat tccgggaga gaatcaaag ataaagtct 
      tgccggaag ccgtcaact ccgtaattg aggaagaat 
      taaacaaac tcaatttct tggaaagtc acagttcgg 
      agtttaatg tcacgcgga actacctcg actggttga 
      cgtcactgc cttgggggg tttacagcg aggagaatt 
      taaatttgc aaagagcgt cggaaattc tagatcagg 
      atcattacg gaatggagg atattaaac gacgcattt 
      tggaattta tcgccgtta gtcagttaa aaggttcga 
      ctcaaggga aaattttgt gtttccatg gacctcccg 
      gtgtcggaa aaactagca tcgccaggt cgatcgcgc 
      gtgccttga accgggagt attttaggt tttcggtgg 
      gtggtatga ccgatgtgg ccgaaatta agggccata 
      ggaggacct acgtggggg ccatgcccg gaaaagtca 
      tccagtgtt tgaagaaaa cgcgcaccg aaaatcccc 
      taattctca ttgatgagg tcgacaaga tcggcaagg 
      gatacaccg gcgacccga gttcggccc tcctcgaac 
      ttctggacc cggaacaaa atgcgaatt tcctcgacc 
      attacctcg atgttccgg ttgatttat ccaaagtat 
      tgttcattt gtactgcga acgttgtcg agaccattc 
      ccgagcctt tgcgcgacc gtatggaaa tgatagata 
      tgtcgggtt acgtagcag aggagaaac tcgcaattg 
      cgaccaaat acctcctgc cacaagcaa tgaaagata 
      gcggtttga acgaccaac atatcaaaa tcgacgacg 
      aggccctaa acaccctca tcaagagtt actgtcgcg 
      aatcaggcg ttcgaaact tgcagaaac acatcgaga 
      aagtggtcc gaaaagttg cctacaaag tcgtgaaag 
      aagaaacga actttgtcg aggtctcca atgcgaatt 
      tggccgagt ttgtgggca aaccggtgt tttcgcaag 
      agaggatgt accccacga cgcccccgg gggtcgtca 
      tggggctgg cgtggactg ctatggggg ggtcaactc 
      tctacattg agaccacaa cgcgcaaaa ttgcagcag 
      agaaggaga cagacgggt ctttggaat taacggggc 
      atttggggg acgttatga aagaatcgg ccaaaatcg 
      ccctgactg tggccagga attatctct acaaaatag 
      acagcacta ataaatttt tgcaaagta gtcatttac 
      acttgcatg tgccggaag gcgcaacgc cgaaagatg 
      gccctagtg cgggttgta caattgtca ctgctttgt 
      tatcgctgg ctaagaatg aaccaatca ggcaggatg 
      tagcaatga cgggggaga tttcactga cggggaagg 
      ttttaccgg tcggaggga ttaaagaaa agacaattg 
      cggccaaaa gatctggag tcaagtgta taatactgc 
      cagaggaaa acaagaaag atttcaatg atcttccga 
      aatttatca ccgacgggt tggaagtac atttcgtta 
      gtacttacg acgaggttt ataatattg tttttaata 
      aaaatcttt gattgtgat agtgttcac ttttttata 
      cagacaagt gttttaatt gatttgtat aaaaatcgt 
      atttattgt acaaaatag tcgaataaa gtttggatt 
      atttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY