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Gene information
General information
TC000558 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9845353..9856928
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000558
    iBeetle screen iB_00126

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000558 RA
      MFLRLYFVT WVSFALIDL LYRKALVAC IPTLNGRYT 
RDIDNFKLT VPFRTSRGG EFATFQIPS YYKQNFNKK 
RRKRSLDDP ELIQYVVNL DGKQYEAQL WPNHNLISP 
DAIFEKRRP KLSVQERKI RKIDNDKMC HYTGYIKGE 
EGSKVALST CDGLAGYIT VREKRFFIE PVQEHAPNA 
KGQHLHIIY ERYPQEGDL RTSQCGTST NWEDAWRKR 
FFEKYSRGE FEKRSTESV HRYLETMVV CDKKFLEFH 
KNIDQETYV LTVMNMVAD FYHDSSSGN MMDVVVVRI 
LYLDKEEEE IDLVINPNA DDTLASFCK WQQKINPKD 
LANPNHHDI AVLLTRYDI CADNMSNCG LMGLAYVAS 
ACSGNEPCA INEDGGLVL GIVVAHEMG HVMGCAHDK 
EGESSCPAQ DEDKSFFVM APYVHLYTT RWSTCSRGF 
ITSLFENGL GDCLNDEPQ ISLYKDKEV LPGVVYDAE 
QQCKMWMPN STLCGFGQE NICEILLCE SKPEECQTK 
EEPAADGTK CGENKWCYR KKCVQMGQR PQAINGGWG 
KWSSFSECS RSCGGGVQT ATRECDNPV PKHRGRYCI 
GERKKIKVC NMDPCPPGS PSFREMQCQ EHNKIPFQD 
KLHQWKAFF KEEEPCVLY CINENRVFV KLEPRAKDG 
TKCKPGTHN MCISGACKK VGCDGEINS EAVEDICGI 
CNGDGTQCK IVDETYTAT GGRDYVKVA AVPVGSRNV 
LFEELAPAV NTLAVSDAS ERRFFLNGG NHEERDGNK 
MFGETEGIY THADEREKL FIHGPLTED IVLFVVFYS 
NANPGYHYK YAEPSLDTS YVPKYHWEQ TDWEECSAK 
CGGGTQAAR WVCVEEKAG KVSDSFCES QGSHPMDTR 
ECNTKPCTT KWKVGQWGK CHACKFKSG VRMREVECV 
RESPHPGAD DILVEDGEC KETKPGTRE LCNSHKKCK 
SRRFTDGLP DELMEDVWK QIHYVRFTR EDKVNSEKE 
GEKNTCGES GKTSKPVKL NVGQIVKDR IPPTEIKVI 
EVPLKQSHL SLNLSDTAF ETMGDQVGD SIDTSKARI 
ATGKDAVKT LKKLQNGGP EQCTEEQKE KT
    CDS sequence
    • >TC000558 RA
      atgttttta cgtctctac tttgtgact tgggtgtct 
tttgccctc atcgattta ttgtaccgc aaggctttg 
gtcgcttgc attcccact cttaatggt cgctacacg 
agggacatt gacaatttt aaattgact gtccctttc 
agaacatca cgcggtggt gagttcgca acattccaa 
ataccttct tactacaaa cagaatttc aacaagaaa 
cgacgaaaa cgatcgctg gatgatcca gaactcatc 
cagtacgtt gtaaattta gacggtaaa caatacgaa 
gcacagctc tggcccaac cataatctc atctcaccg 
gacgcaatt ttcgaaaaa cgcagacct aaattaagc 
gttcaggaa cgcaaaatt cgaaaaatc gacaatgac 
aaaatgtgc cactacacc ggctatatt aagggagaa 
gaaggttcg aaagttgca ctttcgact tgcgatggt 
ttggcaggt tacattaca gtgcgcgaa aaacgcttt 
tttatagaa ccggtccag gaacatgcc cctaatgca 
aaaggccaa catcttcat attatctat gagcggtat 
ccgcaggaa ggcgatttg cgaacaagt cagtgtggc 
acttccacc aactgggaa gacgcttgg aggaagcgc 
ttcttcgaa aaatacagt cgtggcgag tttgagaaa 
agaagcacc gaaagtgtg catcgctac ttggagacg 
atggttgtc tgtgacaag aaattcctc gagtttcat 
aagaacatc gatcaggaa acttatgtc ctgacagtt 
atgaatatg gtggcggat ttttaccac gattccagt 
agtgggaac atgatggac gtggtcgtg gtaaggatt 
ttgtatctt gataaagaa gaggaggaa atcgatttg 
gtcatcaat cccaatgct gatgacacc ttggctagt 
ttttgcaag tggcagcag aaaattaat cccaaggat 
ttggctaat cccaaccat catgatatt gctgtgtta 
ctgaccagg tacgatata tgtgcagac aatatgtca 
aactgtggc ctcatgggg ttggcgtac gtggccagc 
gcttgcagc ggaaacgaa ccttgtgcc atcaacgaa 
gacggagga ctcgtcctg gggattgtc gtcgcccat 
gagatgggg cacgtcatg ggctgcgct catgataag 
gagggagag tcctcttgt ccggctcag gacgaagac 
aagtccttt ttcgttatg gctccttac gtgcacttg 
tataccact cgatggtcc acttgcagt cggggtttt 
ataacttcc ttattcgag aacggatta ggggactgc 
ctcaatgac gagccccag atttcgctt tacaaggac 
aaggaggtc cttcctggc gtcgtctac gacgcggaa 
cagcagtgc aagatgtgg atgccgaac tcaactctt 
tgcggcttc ggccaggag aacatctgc gagatcctc 
ctgtgcgag agcaagccc gaggagtgc cagaccaag 
gaagaaccg gcagcggac ggaaccaaa tgcggcgag 
aacaagtgg tgttacagg aagaagtgt gttcagatg 
ggccagcgt ccccaggcg ataaacggc ggttggggt 
aaatggagc tcgttttcg gagtgttcg cggagctgc 
ggcggcggc gttcagaca gcgaccaga gaatgcgat 
aacccggtg cccaaacac cgaggccgt tattgcatc 
ggcgagcgg aaaaaaatt aaagtttgc aacatggac 
ccgtgtcct ccgggatcg ccatcgttt agagagatg 
caatgccag gaacacaac aagataccg tttcaagat 
aaattgcac cagtggaag gcgtttttc aaggaagag 
gagccttgc gttttatat tgtataaac gagaaccgt 
gtttttgta aaactggag ccgagagct aaagacgga 
actaaatgc aaacccgga acacacaat atgtgtatc 
agtggggct tgcaagaaa gttggatgt gatggtgaa 
attaattcg gaggctgtt gaggacatt tgtggtatt 
tgtaacggc gatgggact caatgtaaa atagttgat 
gagacgtac acagctact ggtggtcgc gattatgtg 
aaagtggct gcggtcccc gtcggcagt cgcaacgtc 
ctcttcgaa gagctggct cctgctgtc aacactttg 
gcagtgagc gacgcaagc gaacggaga tttttcctc 
aacggtgga aatcatgag gaaagggac ggtaacaaa 
atgtttggt gaaaccgaa gggatttac acgcatgcg 
gatgaaaga gagaagctg ttcatacac gggccgcta 
accgaagat atagttctt ttcgtagtt ttttacagc 
aatgcgaat ccgggatac cactacaag tatgccgag 
ccgtccttg gacacgtct tacgtgccc aaatatcac 
tgggagcag accgactgg gaggaatgc tcggcgaaa 
tgtggagga gggacccaa gccgccagg tgggtgtgc 
gttgaggag aaagcgggc aaagtgagc gactccttc 
tgcgagtcg cagggaagt caccccatg gatacgagg 
gaatgcaac acaaagccc tgcaccacc aagtggaag 
gtgggccaa tggggcaag tgccacgcg tgtaagttc 
aagtccggg gtccggatg cgcgaggtg gaatgtgtc 
cgggaatcg ccgcatcca ggtgccgat gacattctg 
gtcgaagac ggggaatgc aaggagaca aaacccggg 
actcgggaa ttatgcaat tcgcacaaa aagtgcaaa 
agtaggaga ttcaccgat ggtttaccg gacgaattg 
atggaggac gtttggaaa caaattcat tacgtgagg 
tttacgaga gaggacaaa gtaaatagt gaaaaggaa 
ggcgagaaa aatacttgt ggagagtcg ggaaaaaca 
tcaaaaccg gtaaaactt aatgtcggg caaatagta 
aaagatagg attcctcct actgagatt aaagtgata 
gaggtgcca ttaaaacaa tctcatctg tccctcaat 
ttatcagac acggcgttt gaaacgatg ggagatcaa 
gtgggagat agcatagat acatctaag gctagaatt 
gccacaggt aaggatgct gtcaagacg ctgaaaaaa 
ttgcaaaac ggggggcca gagcagtgc acagaggag 
cagaaggag aagacttag
    mRNA sequence
    • >TC000558 RA
      gaggctgac aggccagtt gtcaaactg cctctaaag 
atatatctg cacaagggc aaaggttgc aggattcga 
tccataatg ctatcaaga atgaaactg tcacagcga 
ttggttcca gtttttgac tttttatcc gactgtcaa 
gacagtatg tgacgatgt ttttacgtc tctactttg 
tgacttggg tgtcttttg ccctcatcg atttattgt 
accgcaagg ctttggtcg cttgcattc ccactctta 
atggtcgct acacgaggg acattgaca attttaaat 
tgactgtcc ctttcagaa catcacgcg gtggtgagt 
tcgcaacat tccaaatac cttcttact acaaacaga 
atttcaaca agaaacgac gaaaacgat cgctggatg 
atccagaac tcatccagt acgttgtaa atttagacg 
gtaaacaat acgaagcac agctctggc ccaaccata 
atctcatct caccggacg caattttcg aaaaacgca 
gacctaaat taagcgttc aggaacgca aaattcgaa 
aaatcgaca atgacaaaa tgtgccact acaccggct 
atattaagg gagaagaag gttcgaaag ttgcacttt 
cgacttgcg atggtttgg caggttaca ttacagtgc 
gcgaaaaac gctttttta tagaaccgg tccaggaac 
atgccccta atgcaaaag gccaacatc ttcatatta 
tctatgagc ggtatccgc aggaaggcg atttgcgaa 
caagtcagt gtggcactt ccaccaact gggaagacg 
cttggagga agcgcttct tcgaaaaat acagtcgtg 
gcgagtttg agaaaagaa gcaccgaaa gtgtgcatc 
gctacttgg agacgatgg ttgtctgtg acaagaaat 
tcctcgagt ttcataaga acatcgatc aggaaactt 
atgtcctga cagttatga atatggtgg cggattttt 
accacgatt ccagtagtg ggaacatga tggacgtgg 
tcgtggtaa ggattttgt atcttgata aagaagagg 
aggaaatcg atttggtca tcaatccca atgctgatg 
acaccttgg ctagttttt gcaagtggc agcagaaaa 
ttaatccca aggatttgg ctaatccca accatcatg 
atattgctg tgttactga ccaggtacg atatatgtg 
cagacaata tgtcaaact gtggcctca tggggttgg 
cgtacgtgg ccagcgctt gcagcggaa acgaacctt 
gtgccatca acgaagacg gaggactcg tcctgggga 
ttgtcgtcg cccatgaga tggggcacg tcatgggct 
gcgctcatg ataaggagg gagagtcct cttgtccgg 
ctcaggacg aagacaagt cctttttcg ttatggctc 
cttacgtgc acttgtata ccactcgat ggtccactt 
gcagtcggg gttttataa cttccttat tcgagaacg 
gattagggg actgcctca atgacgagc cccagattt 
cgctttaca aggacaagg aggtccttc ctggcgtcg 
tctacgacg cggaacagc agtgcaaga tgtggatgc 
cgaactcaa ctctttgcg gcttcggcc aggagaaca 
tctgcgaga tcctcctgt gcgagagca agcccgagg 
agtgccaga ccaaggaag aaccggcag cggacggaa 
ccaaatgcg gcgagaaca agtggtgtt acaggaaga 
agtgtgttc agatgggcc agcgtcccc aggcgataa 
acggcggtt ggggtaaat ggagctcgt tttcggagt 
gttcgcgga gctgcggcg gcggcgttc agacagcga 
ccagagaat gcgataacc cggtgccca aacaccgag 
gccgttatt gcatcggcg agcggaaaa aaattaaag 
tttgcaaca tggacccgt gtcctccgg gatcgccat 
cgtttagag agatgcaat gccaggaac acaacaaga 
taccgtttc aagataaat tgcaccagt ggaaggcgt 
ttttcaagg aagaggagc cttgcgttt tatattgta 
taaacgaga accgtgttt ttgtaaaac tggagccga 
gagctaaag acggaacta aatgcaaac ccggaacac 
acaatatgt gtatcagtg gggcttgca agaaagttg 
gatgtgatg gtgaaatta attcggagg ctgttgagg 
acatttgtg gtatttgta acggcgatg ggactcaat 
gtaaaatag ttgatgaga cgtacacag ctactggtg 
gtcgcgatt atgtgaaag tggctgcgg tccccgtcg 
gcagtcgca acgtcctct tcgaagagc tggctcctg 
ctgtcaaca ctttggcag tgagcgacg caagcgaac 
ggagatttt tcctcaacg gtggaaatc atgaggaaa 
gggacggta acaaaatgt ttggtgaaa ccgaaggga 
tttacacgc atgcggatg aaagagaga agctgttca 
tacacgggc cgctaaccg aagatatag ttcttttcg 
tagtttttt acagcaatg cgaatccgg gataccact 
acaagtatg ccgagccgt ccttggaca cgtcttacg 
tgcccaaat atcactggg agcagaccg actgggagg 
aatgctcgg cgaaatgtg gaggaggga cccaagccg 
ccaggtggg tgtgcgttg aggagaaag cgggcaaag 
tgagcgact ccttctgcg agtcgcagg gaagtcacc 
ccatggata cgagggaat gcaacacaa agccctgca 
ccaccaagt ggaaggtgg gccaatggg gcaagtgcc 
acgcgtgta agttcaagt ccggggtcc ggatgcgcg 
aggtggaat gtgtccggg aatcgccgc atccaggtg 
ccgatgaca ttctggtcg aagacgggg aatgcaagg 
agacaaaac ccgggactc gggaattat gcaattcgc 
acaaaaagt gcaaaagta ggagattca ccgatggtt 
taccggacg aattgatgg aggacgttt ggaaacaaa 
ttcattacg tgaggttta cgagagagg acaaagtaa 
atagtgaaa aggaaggcg agaaaaata cttgtggag 
agtcgggaa aaacatcaa aaccggtaa aacttaatg 
tcgggcaaa tagtaaaag ataggattc ctcctactg 
agattaaag tgatagagg tgccattaa aacaatctc 
atctgtccc tcaatttat cagacacgg cgtttgaaa 
cgatgggag atcaagtgg gagatagca tagatacat 
ctaaggcta gaattgcca caggtaagg atgctgtca 
agacgctga aaaaattgc aaaacgggg ggccagagc 
agtgcacag aggagcaga aggagaaga cttagtttt 
gtatatttt tacgtttta ttttaataa agttcttga 
atgagg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY