Status
Please wait ...
Gene information
General information
TC000558 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9845353..9856928
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000558
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00126

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000558 RA
      MFLRLYFVT WVSFALIDL LYRKALVAC IPTLNGRYT 
      RDIDNFKLT VPFRTSRGG EFATFQIPS YYKQNFNKK 
      RRKRSLDDP ELIQYVVNL DGKQYEAQL WPNHNLISP 
      DAIFEKRRP KLSVQERKI RKIDNDKMC HYTGYIKGE 
      EGSKVALST CDGLAGYIT VREKRFFIE PVQEHAPNA 
      KGQHLHIIY ERYPQEGDL RTSQCGTST NWEDAWRKR 
      FFEKYSRGE FEKRSTESV HRYLETMVV CDKKFLEFH 
      KNIDQETYV LTVMNMVAD FYHDSSSGN MMDVVVVRI 
      LYLDKEEEE IDLVINPNA DDTLASFCK WQQKINPKD 
      LANPNHHDI AVLLTRYDI CADNMSNCG LMGLAYVAS 
      ACSGNEPCA INEDGGLVL GIVVAHEMG HVMGCAHDK 
      EGESSCPAQ DEDKSFFVM APYVHLYTT RWSTCSRGF 
      ITSLFENGL GDCLNDEPQ ISLYKDKEV LPGVVYDAE 
      QQCKMWMPN STLCGFGQE NICEILLCE SKPEECQTK 
      EEPAADGTK CGENKWCYR KKCVQMGQR PQAINGGWG 
      KWSSFSECS RSCGGGVQT ATRECDNPV PKHRGRYCI 
      GERKKIKVC NMDPCPPGS PSFREMQCQ EHNKIPFQD 
      KLHQWKAFF KEEEPCVLY CINENRVFV KLEPRAKDG 
      TKCKPGTHN MCISGACKK VGCDGEINS EAVEDICGI 
      CNGDGTQCK IVDETYTAT GGRDYVKVA AVPVGSRNV 
      LFEELAPAV NTLAVSDAS ERRFFLNGG NHEERDGNK 
      MFGETEGIY THADEREKL FIHGPLTED IVLFVVFYS 
      NANPGYHYK YAEPSLDTS YVPKYHWEQ TDWEECSAK 
      CGGGTQAAR WVCVEEKAG KVSDSFCES QGSHPMDTR 
      ECNTKPCTT KWKVGQWGK CHACKFKSG VRMREVECV 
      RESPHPGAD DILVEDGEC KETKPGTRE LCNSHKKCK 
      SRRFTDGLP DELMEDVWK QIHYVRFTR EDKVNSEKE 
      GEKNTCGES GKTSKPVKL NVGQIVKDR IPPTEIKVI 
      EVPLKQSHL SLNLSDTAF ETMGDQVGD SIDTSKARI 
      ATGKDAVKT LKKLQNGGP EQCTEEQKE KT
    CDS sequence
    • >TC000558 RA
      atgttttta cgtctctac tttgtgact tgggtgtct 
      tttgccctc atcgattta ttgtaccgc aaggctttg 
      gtcgcttgc attcccact cttaatggt cgctacacg 
      agggacatt gacaatttt aaattgact gtccctttc 
      agaacatca cgcggtggt gagttcgca acattccaa 
      ataccttct tactacaaa cagaatttc aacaagaaa 
      cgacgaaaa cgatcgctg gatgatcca gaactcatc 
      cagtacgtt gtaaattta gacggtaaa caatacgaa 
      gcacagctc tggcccaac cataatctc atctcaccg 
      gacgcaatt ttcgaaaaa cgcagacct aaattaagc 
      gttcaggaa cgcaaaatt cgaaaaatc gacaatgac 
      aaaatgtgc cactacacc ggctatatt aagggagaa 
      gaaggttcg aaagttgca ctttcgact tgcgatggt 
      ttggcaggt tacattaca gtgcgcgaa aaacgcttt 
      tttatagaa ccggtccag gaacatgcc cctaatgca 
      aaaggccaa catcttcat attatctat gagcggtat 
      ccgcaggaa ggcgatttg cgaacaagt cagtgtggc 
      acttccacc aactgggaa gacgcttgg aggaagcgc 
      ttcttcgaa aaatacagt cgtggcgag tttgagaaa 
      agaagcacc gaaagtgtg catcgctac ttggagacg 
      atggttgtc tgtgacaag aaattcctc gagtttcat 
      aagaacatc gatcaggaa acttatgtc ctgacagtt 
      atgaatatg gtggcggat ttttaccac gattccagt 
      agtgggaac atgatggac gtggtcgtg gtaaggatt 
      ttgtatctt gataaagaa gaggaggaa atcgatttg 
      gtcatcaat cccaatgct gatgacacc ttggctagt 
      ttttgcaag tggcagcag aaaattaat cccaaggat 
      ttggctaat cccaaccat catgatatt gctgtgtta 
      ctgaccagg tacgatata tgtgcagac aatatgtca 
      aactgtggc ctcatgggg ttggcgtac gtggccagc 
      gcttgcagc ggaaacgaa ccttgtgcc atcaacgaa 
      gacggagga ctcgtcctg gggattgtc gtcgcccat 
      gagatgggg cacgtcatg ggctgcgct catgataag 
      gagggagag tcctcttgt ccggctcag gacgaagac 
      aagtccttt ttcgttatg gctccttac gtgcacttg 
      tataccact cgatggtcc acttgcagt cggggtttt 
      ataacttcc ttattcgag aacggatta ggggactgc 
      ctcaatgac gagccccag atttcgctt tacaaggac 
      aaggaggtc cttcctggc gtcgtctac gacgcggaa 
      cagcagtgc aagatgtgg atgccgaac tcaactctt 
      tgcggcttc ggccaggag aacatctgc gagatcctc 
      ctgtgcgag agcaagccc gaggagtgc cagaccaag 
      gaagaaccg gcagcggac ggaaccaaa tgcggcgag 
      aacaagtgg tgttacagg aagaagtgt gttcagatg 
      ggccagcgt ccccaggcg ataaacggc ggttggggt 
      aaatggagc tcgttttcg gagtgttcg cggagctgc 
      ggcggcggc gttcagaca gcgaccaga gaatgcgat 
      aacccggtg cccaaacac cgaggccgt tattgcatc 
      ggcgagcgg aaaaaaatt aaagtttgc aacatggac 
      ccgtgtcct ccgggatcg ccatcgttt agagagatg 
      caatgccag gaacacaac aagataccg tttcaagat 
      aaattgcac cagtggaag gcgtttttc aaggaagag 
      gagccttgc gttttatat tgtataaac gagaaccgt 
      gtttttgta aaactggag ccgagagct aaagacgga 
      actaaatgc aaacccgga acacacaat atgtgtatc 
      agtggggct tgcaagaaa gttggatgt gatggtgaa 
      attaattcg gaggctgtt gaggacatt tgtggtatt 
      tgtaacggc gatgggact caatgtaaa atagttgat 
      gagacgtac acagctact ggtggtcgc gattatgtg 
      aaagtggct gcggtcccc gtcggcagt cgcaacgtc 
      ctcttcgaa gagctggct cctgctgtc aacactttg 
      gcagtgagc gacgcaagc gaacggaga tttttcctc 
      aacggtgga aatcatgag gaaagggac ggtaacaaa 
      atgtttggt gaaaccgaa gggatttac acgcatgcg 
      gatgaaaga gagaagctg ttcatacac gggccgcta 
      accgaagat atagttctt ttcgtagtt ttttacagc 
      aatgcgaat ccgggatac cactacaag tatgccgag 
      ccgtccttg gacacgtct tacgtgccc aaatatcac 
      tgggagcag accgactgg gaggaatgc tcggcgaaa 
      tgtggagga gggacccaa gccgccagg tgggtgtgc 
      gttgaggag aaagcgggc aaagtgagc gactccttc 
      tgcgagtcg cagggaagt caccccatg gatacgagg 
      gaatgcaac acaaagccc tgcaccacc aagtggaag 
      gtgggccaa tggggcaag tgccacgcg tgtaagttc 
      aagtccggg gtccggatg cgcgaggtg gaatgtgtc 
      cgggaatcg ccgcatcca ggtgccgat gacattctg 
      gtcgaagac ggggaatgc aaggagaca aaacccggg 
      actcgggaa ttatgcaat tcgcacaaa aagtgcaaa 
      agtaggaga ttcaccgat ggtttaccg gacgaattg 
      atggaggac gtttggaaa caaattcat tacgtgagg 
      tttacgaga gaggacaaa gtaaatagt gaaaaggaa 
      ggcgagaaa aatacttgt ggagagtcg ggaaaaaca 
      tcaaaaccg gtaaaactt aatgtcggg caaatagta 
      aaagatagg attcctcct actgagatt aaagtgata 
      gaggtgcca ttaaaacaa tctcatctg tccctcaat 
      ttatcagac acggcgttt gaaacgatg ggagatcaa 
      gtgggagat agcatagat acatctaag gctagaatt 
      gccacaggt aaggatgct gtcaagacg ctgaaaaaa 
      ttgcaaaac ggggggcca gagcagtgc acagaggag 
      cagaaggag aagacttag
    mRNA sequence
    • >TC000558 RA
      gaggctgac aggccagtt gtcaaactg cctctaaag 
      atatatctg cacaagggc aaaggttgc aggattcga 
      tccataatg ctatcaaga atgaaactg tcacagcga 
      ttggttcca gtttttgac tttttatcc gactgtcaa 
      gacagtatg tgacgatgt ttttacgtc tctactttg 
      tgacttggg tgtcttttg ccctcatcg atttattgt 
      accgcaagg ctttggtcg cttgcattc ccactctta 
      atggtcgct acacgaggg acattgaca attttaaat 
      tgactgtcc ctttcagaa catcacgcg gtggtgagt 
      tcgcaacat tccaaatac cttcttact acaaacaga 
      atttcaaca agaaacgac gaaaacgat cgctggatg 
      atccagaac tcatccagt acgttgtaa atttagacg 
      gtaaacaat acgaagcac agctctggc ccaaccata 
      atctcatct caccggacg caattttcg aaaaacgca 
      gacctaaat taagcgttc aggaacgca aaattcgaa 
      aaatcgaca atgacaaaa tgtgccact acaccggct 
      atattaagg gagaagaag gttcgaaag ttgcacttt 
      cgacttgcg atggtttgg caggttaca ttacagtgc 
      gcgaaaaac gctttttta tagaaccgg tccaggaac 
      atgccccta atgcaaaag gccaacatc ttcatatta 
      tctatgagc ggtatccgc aggaaggcg atttgcgaa 
      caagtcagt gtggcactt ccaccaact gggaagacg 
      cttggagga agcgcttct tcgaaaaat acagtcgtg 
      gcgagtttg agaaaagaa gcaccgaaa gtgtgcatc 
      gctacttgg agacgatgg ttgtctgtg acaagaaat 
      tcctcgagt ttcataaga acatcgatc aggaaactt 
      atgtcctga cagttatga atatggtgg cggattttt 
      accacgatt ccagtagtg ggaacatga tggacgtgg 
      tcgtggtaa ggattttgt atcttgata aagaagagg 
      aggaaatcg atttggtca tcaatccca atgctgatg 
      acaccttgg ctagttttt gcaagtggc agcagaaaa 
      ttaatccca aggatttgg ctaatccca accatcatg 
      atattgctg tgttactga ccaggtacg atatatgtg 
      cagacaata tgtcaaact gtggcctca tggggttgg 
      cgtacgtgg ccagcgctt gcagcggaa acgaacctt 
      gtgccatca acgaagacg gaggactcg tcctgggga 
      ttgtcgtcg cccatgaga tggggcacg tcatgggct 
      gcgctcatg ataaggagg gagagtcct cttgtccgg 
      ctcaggacg aagacaagt cctttttcg ttatggctc 
      cttacgtgc acttgtata ccactcgat ggtccactt 
      gcagtcggg gttttataa cttccttat tcgagaacg 
      gattagggg actgcctca atgacgagc cccagattt 
      cgctttaca aggacaagg aggtccttc ctggcgtcg 
      tctacgacg cggaacagc agtgcaaga tgtggatgc 
      cgaactcaa ctctttgcg gcttcggcc aggagaaca 
      tctgcgaga tcctcctgt gcgagagca agcccgagg 
      agtgccaga ccaaggaag aaccggcag cggacggaa 
      ccaaatgcg gcgagaaca agtggtgtt acaggaaga 
      agtgtgttc agatgggcc agcgtcccc aggcgataa 
      acggcggtt ggggtaaat ggagctcgt tttcggagt 
      gttcgcgga gctgcggcg gcggcgttc agacagcga 
      ccagagaat gcgataacc cggtgccca aacaccgag 
      gccgttatt gcatcggcg agcggaaaa aaattaaag 
      tttgcaaca tggacccgt gtcctccgg gatcgccat 
      cgtttagag agatgcaat gccaggaac acaacaaga 
      taccgtttc aagataaat tgcaccagt ggaaggcgt 
      ttttcaagg aagaggagc cttgcgttt tatattgta 
      taaacgaga accgtgttt ttgtaaaac tggagccga 
      gagctaaag acggaacta aatgcaaac ccggaacac 
      acaatatgt gtatcagtg gggcttgca agaaagttg 
      gatgtgatg gtgaaatta attcggagg ctgttgagg 
      acatttgtg gtatttgta acggcgatg ggactcaat 
      gtaaaatag ttgatgaga cgtacacag ctactggtg 
      gtcgcgatt atgtgaaag tggctgcgg tccccgtcg 
      gcagtcgca acgtcctct tcgaagagc tggctcctg 
      ctgtcaaca ctttggcag tgagcgacg caagcgaac 
      ggagatttt tcctcaacg gtggaaatc atgaggaaa 
      gggacggta acaaaatgt ttggtgaaa ccgaaggga 
      tttacacgc atgcggatg aaagagaga agctgttca 
      tacacgggc cgctaaccg aagatatag ttcttttcg 
      tagtttttt acagcaatg cgaatccgg gataccact 
      acaagtatg ccgagccgt ccttggaca cgtcttacg 
      tgcccaaat atcactggg agcagaccg actgggagg 
      aatgctcgg cgaaatgtg gaggaggga cccaagccg 
      ccaggtggg tgtgcgttg aggagaaag cgggcaaag 
      tgagcgact ccttctgcg agtcgcagg gaagtcacc 
      ccatggata cgagggaat gcaacacaa agccctgca 
      ccaccaagt ggaaggtgg gccaatggg gcaagtgcc 
      acgcgtgta agttcaagt ccggggtcc ggatgcgcg 
      aggtggaat gtgtccggg aatcgccgc atccaggtg 
      ccgatgaca ttctggtcg aagacgggg aatgcaagg 
      agacaaaac ccgggactc gggaattat gcaattcgc 
      acaaaaagt gcaaaagta ggagattca ccgatggtt 
      taccggacg aattgatgg aggacgttt ggaaacaaa 
      ttcattacg tgaggttta cgagagagg acaaagtaa 
      atagtgaaa aggaaggcg agaaaaata cttgtggag 
      agtcgggaa aaacatcaa aaccggtaa aacttaatg 
      tcgggcaaa tagtaaaag ataggattc ctcctactg 
      agattaaag tgatagagg tgccattaa aacaatctc 
      atctgtccc tcaatttat cagacacgg cgtttgaaa 
      cgatgggag atcaagtgg gagatagca tagatacat 
      ctaaggcta gaattgcca caggtaagg atgctgtca 
      agacgctga aaaaattgc aaaacgggg ggccagagc 
      agtgcacag aggagcaga aggagaaga cttagtttt 
      gtatatttt tacgtttta ttttaataa agttcttga 
      atgagg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY