| General information | |||
| TC000558 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 9845353..9856928 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC000558 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00126 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC000558 RA
MFLRLYFVT WVSFALIDL LYRKALVAC IPTLNGRYT RDIDNFKLT VPFRTSRGG EFATFQIPS YYKQNFNKK RRKRSLDDP ELIQYVVNL DGKQYEAQL WPNHNLISP DAIFEKRRP KLSVQERKI RKIDNDKMC HYTGYIKGE EGSKVALST CDGLAGYIT VREKRFFIE PVQEHAPNA KGQHLHIIY ERYPQEGDL RTSQCGTST NWEDAWRKR FFEKYSRGE FEKRSTESV HRYLETMVV CDKKFLEFH KNIDQETYV LTVMNMVAD FYHDSSSGN MMDVVVVRI LYLDKEEEE IDLVINPNA DDTLASFCK WQQKINPKD LANPNHHDI AVLLTRYDI CADNMSNCG LMGLAYVAS ACSGNEPCA INEDGGLVL GIVVAHEMG HVMGCAHDK EGESSCPAQ DEDKSFFVM APYVHLYTT RWSTCSRGF ITSLFENGL GDCLNDEPQ ISLYKDKEV LPGVVYDAE QQCKMWMPN STLCGFGQE NICEILLCE SKPEECQTK EEPAADGTK CGENKWCYR KKCVQMGQR PQAINGGWG KWSSFSECS RSCGGGVQT ATRECDNPV PKHRGRYCI GERKKIKVC NMDPCPPGS PSFREMQCQ EHNKIPFQD KLHQWKAFF KEEEPCVLY CINENRVFV KLEPRAKDG TKCKPGTHN MCISGACKK VGCDGEINS EAVEDICGI CNGDGTQCK IVDETYTAT GGRDYVKVA AVPVGSRNV LFEELAPAV NTLAVSDAS ERRFFLNGG NHEERDGNK MFGETEGIY THADEREKL FIHGPLTED IVLFVVFYS NANPGYHYK YAEPSLDTS YVPKYHWEQ TDWEECSAK CGGGTQAAR WVCVEEKAG KVSDSFCES QGSHPMDTR ECNTKPCTT KWKVGQWGK CHACKFKSG VRMREVECV RESPHPGAD DILVEDGEC KETKPGTRE LCNSHKKCK SRRFTDGLP DELMEDVWK QIHYVRFTR EDKVNSEKE GEKNTCGES GKTSKPVKL NVGQIVKDR IPPTEIKVI EVPLKQSHL SLNLSDTAF ETMGDQVGD SIDTSKARI ATGKDAVKT LKKLQNGGP EQCTEEQKE KT
- >TC000558 RA
atgttttta cgtctctac tttgtgact tgggtgtct tttgccctc atcgattta ttgtaccgc aaggctttg gtcgcttgc attcccact cttaatggt cgctacacg agggacatt gacaatttt aaattgact gtccctttc agaacatca cgcggtggt gagttcgca acattccaa ataccttct tactacaaa cagaatttc aacaagaaa cgacgaaaa cgatcgctg gatgatcca gaactcatc cagtacgtt gtaaattta gacggtaaa caatacgaa gcacagctc tggcccaac cataatctc atctcaccg gacgcaatt ttcgaaaaa cgcagacct aaattaagc gttcaggaa cgcaaaatt cgaaaaatc gacaatgac aaaatgtgc cactacacc ggctatatt aagggagaa gaaggttcg aaagttgca ctttcgact tgcgatggt ttggcaggt tacattaca gtgcgcgaa aaacgcttt tttatagaa ccggtccag gaacatgcc cctaatgca aaaggccaa catcttcat attatctat gagcggtat ccgcaggaa ggcgatttg cgaacaagt cagtgtggc acttccacc aactgggaa gacgcttgg aggaagcgc ttcttcgaa aaatacagt cgtggcgag tttgagaaa agaagcacc gaaagtgtg catcgctac ttggagacg atggttgtc tgtgacaag aaattcctc gagtttcat aagaacatc gatcaggaa acttatgtc ctgacagtt atgaatatg gtggcggat ttttaccac gattccagt agtgggaac atgatggac gtggtcgtg gtaaggatt ttgtatctt gataaagaa gaggaggaa atcgatttg gtcatcaat cccaatgct gatgacacc ttggctagt ttttgcaag tggcagcag aaaattaat cccaaggat ttggctaat cccaaccat catgatatt gctgtgtta ctgaccagg tacgatata tgtgcagac aatatgtca aactgtggc ctcatgggg ttggcgtac gtggccagc gcttgcagc ggaaacgaa ccttgtgcc atcaacgaa gacggagga ctcgtcctg gggattgtc gtcgcccat gagatgggg cacgtcatg ggctgcgct catgataag gagggagag tcctcttgt ccggctcag gacgaagac aagtccttt ttcgttatg gctccttac gtgcacttg tataccact cgatggtcc acttgcagt cggggtttt ataacttcc ttattcgag aacggatta ggggactgc ctcaatgac gagccccag atttcgctt tacaaggac aaggaggtc cttcctggc gtcgtctac gacgcggaa cagcagtgc aagatgtgg atgccgaac tcaactctt tgcggcttc ggccaggag aacatctgc gagatcctc ctgtgcgag agcaagccc gaggagtgc cagaccaag gaagaaccg gcagcggac ggaaccaaa tgcggcgag aacaagtgg tgttacagg aagaagtgt gttcagatg ggccagcgt ccccaggcg ataaacggc ggttggggt aaatggagc tcgttttcg gagtgttcg cggagctgc ggcggcggc gttcagaca gcgaccaga gaatgcgat aacccggtg cccaaacac cgaggccgt tattgcatc ggcgagcgg aaaaaaatt aaagtttgc aacatggac ccgtgtcct ccgggatcg ccatcgttt agagagatg caatgccag gaacacaac aagataccg tttcaagat aaattgcac cagtggaag gcgtttttc aaggaagag gagccttgc gttttatat tgtataaac gagaaccgt gtttttgta aaactggag ccgagagct aaagacgga actaaatgc aaacccgga acacacaat atgtgtatc agtggggct tgcaagaaa gttggatgt gatggtgaa attaattcg gaggctgtt gaggacatt tgtggtatt tgtaacggc gatgggact caatgtaaa atagttgat gagacgtac acagctact ggtggtcgc gattatgtg aaagtggct gcggtcccc gtcggcagt cgcaacgtc ctcttcgaa gagctggct cctgctgtc aacactttg gcagtgagc gacgcaagc gaacggaga tttttcctc aacggtgga aatcatgag gaaagggac ggtaacaaa atgtttggt gaaaccgaa gggatttac acgcatgcg gatgaaaga gagaagctg ttcatacac gggccgcta accgaagat atagttctt ttcgtagtt ttttacagc aatgcgaat ccgggatac cactacaag tatgccgag ccgtccttg gacacgtct tacgtgccc aaatatcac tgggagcag accgactgg gaggaatgc tcggcgaaa tgtggagga gggacccaa gccgccagg tgggtgtgc gttgaggag aaagcgggc aaagtgagc gactccttc tgcgagtcg cagggaagt caccccatg gatacgagg gaatgcaac acaaagccc tgcaccacc aagtggaag gtgggccaa tggggcaag tgccacgcg tgtaagttc aagtccggg gtccggatg cgcgaggtg gaatgtgtc cgggaatcg ccgcatcca ggtgccgat gacattctg gtcgaagac ggggaatgc aaggagaca aaacccggg actcgggaa ttatgcaat tcgcacaaa aagtgcaaa agtaggaga ttcaccgat ggtttaccg gacgaattg atggaggac gtttggaaa caaattcat tacgtgagg tttacgaga gaggacaaa gtaaatagt gaaaaggaa ggcgagaaa aatacttgt ggagagtcg ggaaaaaca tcaaaaccg gtaaaactt aatgtcggg caaatagta aaagatagg attcctcct actgagatt aaagtgata gaggtgcca ttaaaacaa tctcatctg tccctcaat ttatcagac acggcgttt gaaacgatg ggagatcaa gtgggagat agcatagat acatctaag gctagaatt gccacaggt aaggatgct gtcaagacg ctgaaaaaa ttgcaaaac ggggggcca gagcagtgc acagaggag cagaaggag aagacttag
- >TC000558 RA
gaggctgac aggccagtt gtcaaactg cctctaaag atatatctg cacaagggc aaaggttgc aggattcga tccataatg ctatcaaga atgaaactg tcacagcga ttggttcca gtttttgac tttttatcc gactgtcaa gacagtatg tgacgatgt ttttacgtc tctactttg tgacttggg tgtcttttg ccctcatcg atttattgt accgcaagg ctttggtcg cttgcattc ccactctta atggtcgct acacgaggg acattgaca attttaaat tgactgtcc ctttcagaa catcacgcg gtggtgagt tcgcaacat tccaaatac cttcttact acaaacaga atttcaaca agaaacgac gaaaacgat cgctggatg atccagaac tcatccagt acgttgtaa atttagacg gtaaacaat acgaagcac agctctggc ccaaccata atctcatct caccggacg caattttcg aaaaacgca gacctaaat taagcgttc aggaacgca aaattcgaa aaatcgaca atgacaaaa tgtgccact acaccggct atattaagg gagaagaag gttcgaaag ttgcacttt cgacttgcg atggtttgg caggttaca ttacagtgc gcgaaaaac gctttttta tagaaccgg tccaggaac atgccccta atgcaaaag gccaacatc ttcatatta tctatgagc ggtatccgc aggaaggcg atttgcgaa caagtcagt gtggcactt ccaccaact gggaagacg cttggagga agcgcttct tcgaaaaat acagtcgtg gcgagtttg agaaaagaa gcaccgaaa gtgtgcatc gctacttgg agacgatgg ttgtctgtg acaagaaat tcctcgagt ttcataaga acatcgatc aggaaactt atgtcctga cagttatga atatggtgg cggattttt accacgatt ccagtagtg ggaacatga tggacgtgg tcgtggtaa ggattttgt atcttgata aagaagagg aggaaatcg atttggtca tcaatccca atgctgatg acaccttgg ctagttttt gcaagtggc agcagaaaa ttaatccca aggatttgg ctaatccca accatcatg atattgctg tgttactga ccaggtacg atatatgtg cagacaata tgtcaaact gtggcctca tggggttgg cgtacgtgg ccagcgctt gcagcggaa acgaacctt gtgccatca acgaagacg gaggactcg tcctgggga ttgtcgtcg cccatgaga tggggcacg tcatgggct gcgctcatg ataaggagg gagagtcct cttgtccgg ctcaggacg aagacaagt cctttttcg ttatggctc cttacgtgc acttgtata ccactcgat ggtccactt gcagtcggg gttttataa cttccttat tcgagaacg gattagggg actgcctca atgacgagc cccagattt cgctttaca aggacaagg aggtccttc ctggcgtcg tctacgacg cggaacagc agtgcaaga tgtggatgc cgaactcaa ctctttgcg gcttcggcc aggagaaca tctgcgaga tcctcctgt gcgagagca agcccgagg agtgccaga ccaaggaag aaccggcag cggacggaa ccaaatgcg gcgagaaca agtggtgtt acaggaaga agtgtgttc agatgggcc agcgtcccc aggcgataa acggcggtt ggggtaaat ggagctcgt tttcggagt gttcgcgga gctgcggcg gcggcgttc agacagcga ccagagaat gcgataacc cggtgccca aacaccgag gccgttatt gcatcggcg agcggaaaa aaattaaag tttgcaaca tggacccgt gtcctccgg gatcgccat cgtttagag agatgcaat gccaggaac acaacaaga taccgtttc aagataaat tgcaccagt ggaaggcgt ttttcaagg aagaggagc cttgcgttt tatattgta taaacgaga accgtgttt ttgtaaaac tggagccga gagctaaag acggaacta aatgcaaac ccggaacac acaatatgt gtatcagtg gggcttgca agaaagttg gatgtgatg gtgaaatta attcggagg ctgttgagg acatttgtg gtatttgta acggcgatg ggactcaat gtaaaatag ttgatgaga cgtacacag ctactggtg gtcgcgatt atgtgaaag tggctgcgg tccccgtcg gcagtcgca acgtcctct tcgaagagc tggctcctg ctgtcaaca ctttggcag tgagcgacg caagcgaac ggagatttt tcctcaacg gtggaaatc atgaggaaa gggacggta acaaaatgt ttggtgaaa ccgaaggga tttacacgc atgcggatg aaagagaga agctgttca tacacgggc cgctaaccg aagatatag ttcttttcg tagtttttt acagcaatg cgaatccgg gataccact acaagtatg ccgagccgt ccttggaca cgtcttacg tgcccaaat atcactggg agcagaccg actgggagg aatgctcgg cgaaatgtg gaggaggga cccaagccg ccaggtggg tgtgcgttg aggagaaag cgggcaaag tgagcgact ccttctgcg agtcgcagg gaagtcacc ccatggata cgagggaat gcaacacaa agccctgca ccaccaagt ggaaggtgg gccaatggg gcaagtgcc acgcgtgta agttcaagt ccggggtcc ggatgcgcg aggtggaat gtgtccggg aatcgccgc atccaggtg ccgatgaca ttctggtcg aagacgggg aatgcaagg agacaaaac ccgggactc gggaattat gcaattcgc acaaaaagt gcaaaagta ggagattca ccgatggtt taccggacg aattgatgg aggacgttt ggaaacaaa ttcattacg tgaggttta cgagagagg acaaagtaa atagtgaaa aggaaggcg agaaaaata cttgtggag agtcgggaa aaacatcaa aaccggtaa aacttaatg tcgggcaaa tagtaaaag ataggattc ctcctactg agattaaag tgatagagg tgccattaa aacaatctc atctgtccc tcaatttat cagacacgg cgtttgaaa cgatgggag atcaagtgg gagatagca tagatacat ctaaggcta gaattgcca caggtaagg atgctgtca agacgctga aaaaattgc aaaacgggg ggccagagc agtgcacag aggagcaga aggagaaga cttagtttt gtatatttt tacgtttta ttttaataa agttcttga atgagg
