Status
Please wait ...
Gene information
General information
TC000542 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 4483082..4484525
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000542
    iBeetle screen iB_00125
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupation not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000542 RA
      MGSSHSALN FGAGPAKLP REVLAEVQT ELLSYQDSG 
      MSLMEMSHR GKEYTKINN DAQKAIREL LNVPDNYKV 
      LFMQGGGTG AFAAVALNL MNRTGVADY AVTGTWSSK 
      AAKEAAKYG KVNLVFPKA DKPGSIPPE SSWTLNPDA 
      SYVYYCDNE TVDGVEFPF IPTSPHGVP IVSDMSSSI 
      MTKKIDISK FGCIVAGAQ KNIGPAGVT VVIIREDLL 
      GNPMKICPT VFDFTVISQ QNSVHNTPA TFSVYVMEK 
      VLQWIKKNG GLQAMENQS QDKSKLLYE EMENSNNFY 
      SCPIDENCR SRINVPFRV GGPQGNEEL EAKFLEEAE 
      KLKMFQLKG HRLVGGIRA SLYNAVTRD EVALLVKFM 
      KEFRAQFS
    CDS sequence
    • >TC000542 RA
      atgggaagc agtcattca gccttaaat ttcggagcc 
      ggccctgcc aaactccct cgagaggtg ttagccgag 
      gtccaaacc gagctcctc tcgtaccaa gacagtggg 
      atgagtctc atggaaatg agccatcgt ggcaaagag 
      tacacgaaa atcaacaac gatgcacaa aaggccatt 
      cgagaatta ttgaatgtt ccagacaac tataaggtg 
      ctcttcatg caagggggc gggaccggg gccttcgcc 
      gccgtggcc ttgaatctg atgaaccgc accggcgtt 
      gccgactat gccgtgacg ggcacctgg tcctcgaag 
      gccgccaaa gaggccgca aaatacggc aaagtgaat 
      ctggtgttt ccgaaagcc gacaagcct ggaagcatt 
      ccgcccgaa tcgagctgg acgttgaac cctgacgct 
      tcttatgtc tactactgt gataatgag acagttgat 
      ggagtcgag tttcccttc atcccgacc tcgcctcac 
      ggcgtgccg atcgtcagc gacatgtcc tccagtatt 
      atgacgaag aaaatcgac atttccaag ttcgggtgc 
      atcgtcgcg ggggcgcag aagaacatt gggccggcg 
      ggagtcacc gttgtgatc atccgtgaa gaccttctg 
      ggaaacccc atgaaaatc tgccctaca gtattcgat 
      ttcacagtc atcagccag cagaattcc gtgcataac 
      acgcctgct accttctca gtttacgta atggagaaa 
      gtcctgcag tggatcaag aaaaatggt ggccttcaa 
      gccatggaa aatcagtcg caagacaag agcaagctt 
      ctgtacgaa gaaatggag aactcgaat aatttctac 
      agttgtccg attgatgaa aactgcaga agcaggatt 
      aatgtgccc ttcagggtc gggggcccc caaggcaac 
      gaggaacta gaagcgaaa ttcttggaa gaggcggaa 
      aaacttaaa atgttccaa ttgaaaggt caccgattg 
      gttggtgga attagagcg tcgttatac aacgctgtg 
      acgcgcgat gaagttgcg cttttagtc aaatttatg 
      aaagagttc agagcgcag ttcagttaa
    mRNA sequence
    • >TC000542 RA
      agcagtttg aaagtgcac agtacagct atcagttga 
      taaagttta gtgcaagtg taattatgg gaagcagtc 
      attcagcct taaatttcg gagccggcc ctgccaaac 
      tccctcgag aggtgttag ccgaggtcc aaaccgagc 
      tcctctcgt accaagaca gtgggatga gtctcatgg 
      aaatgagcc atcgtggca aagagtaca cgaaaatca 
      acaacgatg cacaaaagg ccattcgag aattattga 
      atgttccag acaactata aggtgctct tcatgcaag 
      ggggcggga ccggggcct tcgccgccg tggccttga 
      atctgatga accgcaccg gcgttgccg actatgccg 
      tgacgggca cctggtcct cgaaggccg ccaaagagg 
      ccgcaaaat acggcaaag tgaatctgg tgtttccga 
      aagccgaca agcctggaa gcattccgc ccgaatcga 
      gctggacgt tgaaccctg acgcttctt atgtctact 
      actgtgata atgagacag ttgatggag tcgagtttc 
      ccttcatcc cgacctcgc ctcacggcg tgccgatcg 
      tcagcgaca tgtcctcca gtattatga cgaagaaaa 
      tcgacattt ccaagttcg ggtgcatcg tcgcggggg 
      cgcagaaga acattgggc cggcgggag tcaccgttg 
      tgatcatcc gtgaagacc ttctgggaa accccatga 
      aaatctgcc ctacagtat tcgatttca cagtcatca 
      gccagcaga attccgtgc ataacacgc ctgctacct 
      tctcagttt acgtaatgg agaaagtcc tgcagtgga 
      tcaagaaaa atggtggcc ttcaagcca tggaaaatc 
      agtcgcaag acaagagca agcttctgt acgaagaaa 
      tggagaact cgaataatt tctacagtt gtccgattg 
      atgaaaact gcagaagca ggattaatg tgcccttca 
      gggtcgggg gcccccaag gcaacgagg aactagaag 
      cgaaattct tggaagagg cggaaaaac ttaaaatgt 
      tccaattga aaggtcacc gattggttg gtggaatta 
      gagcgtcgt tatacaacg ctgtgacgc gcgatgaag 
      ttgcgcttt tagtcaaat ttatgaaag agttcagag 
      cgcagttca gttaaatta atttttatt taattccca 
      tgtatttat tgtattgta tttttaagt attttagga 
      ataaatatt ttttcaagt t