| General information | |||
| TC000537 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 10107053..10109028 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC000537 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00122 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (19 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (22 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (41 dpi)
- >TC000537 RA
MEFSMKNFK FYPICPTFL VLLSVTCSS EVSRPRGVS LSRASLYNP DRNFLCFDN SKSIPFSQV NDEYCDCPD GSDEPGTSA CPNGVFHCT NAGHKPLNL AASRVNDGI CDCCDGSDE YAGNTVTTC PNICLQLGR HAREEAQKL AEIIKAGKQ LKAELSQKG LRLKEEKKE KLVELQKNK EEAEKVKAE KQRIKDEIE ALENKSLEV YRKLEEEEK QRKAEAEAQ KTRQEATET FTKFDSNQD GLVDIAELQ TRQSFDKDR NGEVSVEEA KYFLNNQES VDLETFISD AWANIKPYL MMDAGLFKP PVPQDGEAE DLQDGDDEH DGAPEGDEG EEEEEPYQP EEEPEETKL QYDEETQKI VDQATAARN EFTDAERAV REIESEIGN INDYLEKDF GPEEEFATL QGECFDYTD HEYIYKLCP FEKATQQPK SGSSETRLG TWARWNGPE DNKYGSMLY DKGQSCWNG PPRSTKVNI VCGTESKVT AVSEPNRCE YLFEFVTPA ACREIPSEA DDLHDEL
- >TC000537 RA
atggagttc tctatgaaa aactttaaa ttttaccca atttgcccg acattttta gtgctgttg agtgtcaca tgcagttcc gaggtgtcc aggcctcgc ggggtttca ctatcgaga gcttcttta tacaatcca gatcgtaat tttttatgt tttgataac agcaaaagc atacctttt tcgcaagta aatgacgag tattgcgac tgtcccgat ggcagcgac gagccaggg acgtcggcg tgccccaat ggggttttt cactgcacc aacgccggc cataaaccc ttgaattta gccgcgagc cgagttaac gacgggatt tgcgactgt tgtgacggg agcgatgaa tacgccgga aacactgtg accacgtgc cccaacatt tgcctgcaa ttggggcga cacgccaga gaggaagcc caaaagctg gcggaaatt atcaaagcc ggcaaacag ctcaaagct gagcttagc cagaaaggt ttgagactg aaagaagag aagaaggag aagttggtg gagttgcaa aagaacaaa gaagaggct gagaaagtt aaagccgag aaacagaga attaaagat gagattgaa gcgcttgaa aacaagtca cttgaagtt taccggaag ctggaagag gaagagaag cagcgcaaa gctgaggcc gaagcacag aaaacgaga caggaggcc actgaaacg ttcacgaaa tttgattcc aatcaggat ggcttagtt gatattgca gagctccag acgagacaa tcatttgat aaggatagg aacggcgaa gtgtcggtg gaagaagct aagtatttt ttgaacaat caagaatcc gtggacctt gaaacattt atatcagac gcttgggct aacattaag ccttatcta atgatggat gctggttta ttcaaacca ccggtcccc caggatggt gaagctgaa gacttgcag gacggagac gacgagcat gacggtgct ccagagggc gatgaagga gaggaggag gaagagcca taccagcct gaggaagaa ccagaagaa acgaaactg cagtacgat gaagaaacg cagaaaatt gtagaccaa gccaccgct gcgcggaac gagtttacc gacgcggag cgcgcagtg agggaaatt gagtcagaa attggcaat attaacgac tacctagag aaggatttt gggcccgag gaagaattt gcgaccctt caaggggaa tgtttcgac tacaccgac catgagtac atttacaaa ctgtgtccc tttgaaaag gcgacacaa cagcccaaa tccggctct tctgagacc agattagga acgtgggcg agatggaac ggccctgaa gacaataaa tacggttcc atgctgtac gacaaaggg cagagctgc tggaatggc cctccgagg tcgacaaaa gtgaatatt gtgtgcggt actgaaagt aaagtgact gcggtttct gaacctaat aggtgcgaa tatttgttc gagtttgta acgcctgca gcttgcaga gaaattcca agtgaagct gatgattta cacgatgaa ttgtag
- >TC000537 RA
aggtgtact tcaattttc actgacgtg tcctttcat ccgcgcatg ttttgtcgc cccaaacgt caacgtttt gaccggtgt tgtgaaacg tgatggagt tctctatga aaaacttta aattttacc caatttgcc cgacatttt tagtgctgt tgagtgtca catgcagtt ccgaggtgt ccaggcctc gcggggttt cactatcga gagcttctt tatacaatc cagatcgta attttttat gttttgata acagcaaaa gcatacctt tttcgcaag taaatgacg agtattgcg actgtcccg atggcagcg acgagccag ggacgtcgg cgtgcccca atggggttt ttcactgca ccaacgccg gccataaac ccttgaatt tagccgcga gccgagtta acgacggga tttgcgact gttgtgacg ggagcgatg aatacgccg gaaacactg tgaccacgt gccccaaca tttgcctgc aattggggc gacacgcca gagaggaag cccaaaagc tggcggaaa ttatcaaag ccggcaaac agctcaaag ctgagctta gccagaaag gtttgagac tgaaagaag agaagaagg agaagttgg tggagttgc aaaagaaca aagaagagg ctgagaaag ttaaagccg agaaacaga gaattaaag atgagattg aagcgcttg aaaacaagt cacttgaag tttaccgga agctggaag aggaagaga agcagcgca aagctgagg ccgaagcac agaaaacga gacaggagg ccactgaaa cgttcacga aatttgatt ccaatcagg atggcttag ttgatattg cagagctcc agacgagac aatcatttg ataaggata ggaacggcg aagtgtcgg tggaagaag ctaagtatt ttttgaaca atcaagaat ccgtggacc ttgaaacat ttatatcag acgcttggg ctaacatta agccttatc taatgatgg atgctggtt tattcaaac caccggtcc cccaggatg gtgaagctg aagacttgc aggacggag acgacgagc atgacggtg ctccagagg gcgatgaag gagaggagg aggaagagc cataccagc ctgaggaag aaccagaag aaacgaaac tgcagtacg atgaagaaa cgcagaaaa ttgtagacc aagccaccg ctgcgcgga acgagttta ccgacgcgg agcgcgcag tgagggaaa ttgagtcag aaattggca atattaacg actacctag agaaggatt ttgggcccg aggaagaat ttgcgaccc ttcaagggg aatgtttcg actacaccg accatgagt acatttaca aactgtgtc cctttgaaa aggcgacac aacagccca aatccggct cttctgaga ccagattag gaacgtggg cgagatgga acggccctg aagacaata aatacggtt ccatgctgt acgacaaag ggcagagct gctggaatg gccctccga ggtcgacaa aagtgaata ttgtgtgcg gtactgaaa gtaaagtga ctgcggttt ctgaaccta ataggtgcg aatatttgt tcgagtttg taacgcctg cagcttgca gagaaattc caagtgaag ctgatgatt tacacgatg aattgtaga gttttctaa ttgtgaatt tataacttt tatataatc catggtagt ttgtacata catttccta aaaatttac agtaatttt caagtattg cgcattgtg agataggta agtttggta gtttgttta ttttgtgtg gcggaaaat cagtgataa tttaagacc aagaacttt gttattagt ggaggatca tgtttaaga aaaaattta aataaaagt tttgaatta aa
