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Gene information
General information
TC000537 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10107053..10109028
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000537
    iBeetle screen iB_00122

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    pupation delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000537 RA
      MEFSMKNFK FYPICPTFL VLLSVTCSS EVSRPRGVS 
      LSRASLYNP DRNFLCFDN SKSIPFSQV NDEYCDCPD 
      GSDEPGTSA CPNGVFHCT NAGHKPLNL AASRVNDGI 
      CDCCDGSDE YAGNTVTTC PNICLQLGR HAREEAQKL 
      AEIIKAGKQ LKAELSQKG LRLKEEKKE KLVELQKNK 
      EEAEKVKAE KQRIKDEIE ALENKSLEV YRKLEEEEK 
      QRKAEAEAQ KTRQEATET FTKFDSNQD GLVDIAELQ 
      TRQSFDKDR NGEVSVEEA KYFLNNQES VDLETFISD 
      AWANIKPYL MMDAGLFKP PVPQDGEAE DLQDGDDEH 
      DGAPEGDEG EEEEEPYQP EEEPEETKL QYDEETQKI 
      VDQATAARN EFTDAERAV REIESEIGN INDYLEKDF 
      GPEEEFATL QGECFDYTD HEYIYKLCP FEKATQQPK 
      SGSSETRLG TWARWNGPE DNKYGSMLY DKGQSCWNG 
      PPRSTKVNI VCGTESKVT AVSEPNRCE YLFEFVTPA 
      ACREIPSEA DDLHDEL
    CDS sequence
    • >TC000537 RA
      atggagttc tctatgaaa aactttaaa ttttaccca 
      atttgcccg acattttta gtgctgttg agtgtcaca 
      tgcagttcc gaggtgtcc aggcctcgc ggggtttca 
      ctatcgaga gcttcttta tacaatcca gatcgtaat 
      tttttatgt tttgataac agcaaaagc atacctttt 
      tcgcaagta aatgacgag tattgcgac tgtcccgat 
      ggcagcgac gagccaggg acgtcggcg tgccccaat 
      ggggttttt cactgcacc aacgccggc cataaaccc 
      ttgaattta gccgcgagc cgagttaac gacgggatt 
      tgcgactgt tgtgacggg agcgatgaa tacgccgga 
      aacactgtg accacgtgc cccaacatt tgcctgcaa 
      ttggggcga cacgccaga gaggaagcc caaaagctg 
      gcggaaatt atcaaagcc ggcaaacag ctcaaagct 
      gagcttagc cagaaaggt ttgagactg aaagaagag 
      aagaaggag aagttggtg gagttgcaa aagaacaaa 
      gaagaggct gagaaagtt aaagccgag aaacagaga 
      attaaagat gagattgaa gcgcttgaa aacaagtca 
      cttgaagtt taccggaag ctggaagag gaagagaag 
      cagcgcaaa gctgaggcc gaagcacag aaaacgaga 
      caggaggcc actgaaacg ttcacgaaa tttgattcc 
      aatcaggat ggcttagtt gatattgca gagctccag 
      acgagacaa tcatttgat aaggatagg aacggcgaa 
      gtgtcggtg gaagaagct aagtatttt ttgaacaat 
      caagaatcc gtggacctt gaaacattt atatcagac 
      gcttgggct aacattaag ccttatcta atgatggat 
      gctggttta ttcaaacca ccggtcccc caggatggt 
      gaagctgaa gacttgcag gacggagac gacgagcat 
      gacggtgct ccagagggc gatgaagga gaggaggag 
      gaagagcca taccagcct gaggaagaa ccagaagaa 
      acgaaactg cagtacgat gaagaaacg cagaaaatt 
      gtagaccaa gccaccgct gcgcggaac gagtttacc 
      gacgcggag cgcgcagtg agggaaatt gagtcagaa 
      attggcaat attaacgac tacctagag aaggatttt 
      gggcccgag gaagaattt gcgaccctt caaggggaa 
      tgtttcgac tacaccgac catgagtac atttacaaa 
      ctgtgtccc tttgaaaag gcgacacaa cagcccaaa 
      tccggctct tctgagacc agattagga acgtgggcg 
      agatggaac ggccctgaa gacaataaa tacggttcc 
      atgctgtac gacaaaggg cagagctgc tggaatggc 
      cctccgagg tcgacaaaa gtgaatatt gtgtgcggt 
      actgaaagt aaagtgact gcggtttct gaacctaat 
      aggtgcgaa tatttgttc gagtttgta acgcctgca 
      gcttgcaga gaaattcca agtgaagct gatgattta 
      cacgatgaa ttgtag
    mRNA sequence
    • >TC000537 RA
      aggtgtact tcaattttc actgacgtg tcctttcat 
      ccgcgcatg ttttgtcgc cccaaacgt caacgtttt 
      gaccggtgt tgtgaaacg tgatggagt tctctatga 
      aaaacttta aattttacc caatttgcc cgacatttt 
      tagtgctgt tgagtgtca catgcagtt ccgaggtgt 
      ccaggcctc gcggggttt cactatcga gagcttctt 
      tatacaatc cagatcgta attttttat gttttgata 
      acagcaaaa gcatacctt tttcgcaag taaatgacg 
      agtattgcg actgtcccg atggcagcg acgagccag 
      ggacgtcgg cgtgcccca atggggttt ttcactgca 
      ccaacgccg gccataaac ccttgaatt tagccgcga 
      gccgagtta acgacggga tttgcgact gttgtgacg 
      ggagcgatg aatacgccg gaaacactg tgaccacgt 
      gccccaaca tttgcctgc aattggggc gacacgcca 
      gagaggaag cccaaaagc tggcggaaa ttatcaaag 
      ccggcaaac agctcaaag ctgagctta gccagaaag 
      gtttgagac tgaaagaag agaagaagg agaagttgg 
      tggagttgc aaaagaaca aagaagagg ctgagaaag 
      ttaaagccg agaaacaga gaattaaag atgagattg 
      aagcgcttg aaaacaagt cacttgaag tttaccgga 
      agctggaag aggaagaga agcagcgca aagctgagg 
      ccgaagcac agaaaacga gacaggagg ccactgaaa 
      cgttcacga aatttgatt ccaatcagg atggcttag 
      ttgatattg cagagctcc agacgagac aatcatttg 
      ataaggata ggaacggcg aagtgtcgg tggaagaag 
      ctaagtatt ttttgaaca atcaagaat ccgtggacc 
      ttgaaacat ttatatcag acgcttggg ctaacatta 
      agccttatc taatgatgg atgctggtt tattcaaac 
      caccggtcc cccaggatg gtgaagctg aagacttgc 
      aggacggag acgacgagc atgacggtg ctccagagg 
      gcgatgaag gagaggagg aggaagagc cataccagc 
      ctgaggaag aaccagaag aaacgaaac tgcagtacg 
      atgaagaaa cgcagaaaa ttgtagacc aagccaccg 
      ctgcgcgga acgagttta ccgacgcgg agcgcgcag 
      tgagggaaa ttgagtcag aaattggca atattaacg 
      actacctag agaaggatt ttgggcccg aggaagaat 
      ttgcgaccc ttcaagggg aatgtttcg actacaccg 
      accatgagt acatttaca aactgtgtc cctttgaaa 
      aggcgacac aacagccca aatccggct cttctgaga 
      ccagattag gaacgtggg cgagatgga acggccctg 
      aagacaata aatacggtt ccatgctgt acgacaaag 
      ggcagagct gctggaatg gccctccga ggtcgacaa 
      aagtgaata ttgtgtgcg gtactgaaa gtaaagtga 
      ctgcggttt ctgaaccta ataggtgcg aatatttgt 
      tcgagtttg taacgcctg cagcttgca gagaaattc 
      caagtgaag ctgatgatt tacacgatg aattgtaga 
      gttttctaa ttgtgaatt tataacttt tatataatc 
      catggtagt ttgtacata catttccta aaaatttac 
      agtaatttt caagtattg cgcattgtg agataggta 
      agtttggta gtttgttta ttttgtgtg gcggaaaat 
      cagtgataa tttaagacc aagaacttt gttattagt 
      ggaggatca tgtttaaga aaaaattta aataaaagt 
      tttgaatta aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY