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Gene information
General information
TC000530 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10141102..10143769
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000530
    iBeetle screen iB_00120

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen musculature partially irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    some embryos seem to have less abdominal segments. check for cuticle phenotype

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    dorsal ridge row partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Maxilla escort bristles are sometimes partially missing
    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen bristle dorsal partiallynot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 100
    Adult morphology
    remarks: later than schedule, hours 100
    iBeetle screen iB_06133

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000530 RA
      MALKNAPRD DFTSGLTHE CGVFGAIGT SDWPNTNSE 
      IAQIICIGL EALQHRGQE SCGVATSEG SEDFHIHKG 
      MGLVKNVFN NDNISKLKG SLGIGHTRY ATRAASAAI 
      NCQPFVVHS MHGSLATAH NGELVNCSS LRKKVLDRG 
      VGLSTHSDS EIITQALCL IPPEGEVNG PDWPARIRH 
      FMQMAPLSY SLVLMLKDR IYAVRDPYG NRPLCLGKI 
      LPPHCDRND ESVQPEGWL VSSESCAFL TITRYVREV 
      LPGEIVEMT HQGVRTVDI VARPANKQL AFCIFEYVY 
      FARPNSIFE GQEVYTVRS HCGRQLAIE HPVEADIVG 
      SVPESGNAA AFGYSKESN IPMGELLTK NTYVGRTFI 
      QPNNRLRQL NVALKFSPI STNVKGKRI ILIDDSIVR 
      GNTIGPIIK LLRRVGAKE VHIRVASPP LKYPCFMGI 
      NIPTREELI ANDLDSRSL ADKVGADSL EYLSVEGLV 
      KAVHRNIEK GAEKGHCTA CLTGEYPEE IPYELQW
    CDS sequence
    • >TC000530 RA
      atggcattg aaaaatgcg cctcgggac gactttact 
      tccggtttg acgcacgaa tgcggcgtt tttggggct 
      atcggcaca agtgactgg cccaacacg aactctgaa 
      atcgctcag ataatctgt atcggtttg gaagccctc 
      cagcatcgg ggtcaagag tcatgcggt gtcgcaaca 
      agcgaagga agcgaagat tttcacatc cacaaaggc 
      atgggcttg gtcaagaac gtcttcaat aacgacaat 
      atcagcaaa ctgaaaggc tcactaggg attgggcac 
      acgagatat gccactagg gccgcttcg gctgctatc 
      aattgccaa ccgtttgtg gtgcactcc atgcacggt 
      tccttggcc acggcgcac aatggcgag ctagtaaac 
      tgctctagt ttgaggaaa aaggttcta gaccggggt 
      gtggggctt tctacgcac tccgacagc gaaattata 
      acacaagct ctgtgctta ataccgcca gaaggcgaa 
      gtcaatggt cccgattgg cccgcaagg atccgtcat 
      ttcatgcaa atggccccc ttgagctac tcgctagtc 
      ctgatgctc aaggaccgc atttacgct gttagggac 
      ccgtacggc aaccggccc ttgtgtctc ggcaaaatt 
      ttgccccct cattgcgac aggaatgac gaaagcgtg 
      cagcctgag ggatggctc gtttcgtcc gaatcgtgc 
      gcctttttg accatcacc agatacgtt cgggaggtt 
      ttaccgggg gaaatcgtc gaaatgaca caccagggt 
      gtgcgaacg gttgatata gtagctcga ccggctaac 
      aaacaacta gctttttgc attttcgaa tatgtttat 
      ttcgcgagg cccaatagt atttttgag gggcaagag 
      gtgtacact gtgcggtcg cactgtggc aggcaattg 
      gccattgaa catccggtt gaagcggac attgtgggg 
      tctgtgccc gaatcgggg aatgcggcc gcttttggc 
      tactccaag gagtcgaat atcccgatg ggggagctt 
      ttgacaaaa aatacgtac gttggcagg acgttcatc 
      caaccgaac aatcggctt cgccagctg aacgtcgcc 
      ttgaagttt agccccata tcgacgaat gtaaaaggc 
      aaaagaatt attcttatt gacgattcg attgtaaga 
      ggcaatact attggtcct atcataaaa ttgttacgt 
      cgcgtgggg gctaaagag gtgcatatt agagtggca 
      agtccgcct ttgaagtac ccctgcttc atgggaatt 
      aatattccg actagagaa gagttgatt gctaatgat 
      ttagattcg agaagtctt gctgataaa gttggagcc 
      gatagtttg gaatatttg agtgtggaa gggcttgtt 
      aaggcggta cacagaaat attgaaaag ggggctgag 
      aaggggcac tgcaccgct tgcttgact ggcgaatac 
      ccagaagaa atcccctat gaattacag tggtaa
    mRNA sequence
    • >TC000530 RA
      cccattcat gggtctcaa aacgtgcaa caggtgttt 
      tacagttat ttgtaacct gaaaccacg tgtgtgcag 
      tttttgaat ttgtaagtt cagcaatgg cattgaaaa 
      atgcgcctc gggacgact ttacttccg gtttgacgc 
      acgaatgcg gcgtttttg gggctatcg gcacaagtg 
      actggccca acacgaact ctgaaatcg ctcagataa 
      tctgtatcg gtttggaag ccctccagc atcggggtc 
      aagagtcat gcggtgtcg caacaagcg aaggaagcg 
      aagattttc acatccaca aaggcatgg gcttggtca 
      agaacgtct tcaataacg acaatatca gcaaactga 
      aaggctcac tagggattg ggcacacga gatatgcca 
      ctagggccg cttcggctg ctatcaatt gccaaccgt 
      ttgtggtgc actccatgc acggttcct tggccacgg 
      cgcacaatg gcgagctag taaactgct ctagtttga 
      ggaaaaagg ttctagacc ggggtgtgg ggctttcta 
      cgcactccg acagcgaaa ttataacac aagctctgt 
      gcttaatac cgccagaag gcgaagtca atggtcccg 
      attggcccg caaggatcc gtcatttca tgcaaatgg 
      cccccttga gctactcgc tagtcctga tgctcaagg 
      accgcattt acgctgtta gggacccgt acggcaacc 
      ggcccttgt gtctcggca aaattttgc cccctcatt 
      gcgacagga atgacgaaa gcgtgcagc ctgagggat 
      ggctcgttt cgtccgaat cgtgcgcct ttttgacca 
      tcaccagat acgttcggg aggttttac cgggggaaa 
      tcgtcgaaa tgacacacc agggtgtgc gaacggttg 
      atatagtag ctcgaccgg ctaacaaac aactagctt 
      tttgcattt tcgaatatg tttatttcg cgaggccca 
      atagtattt ttgaggggc aagaggtgt acactgtgc 
      ggtcgcact gtggcaggc aattggcca ttgaacatc 
      cggttgaag cggacattg tggggtctg tgcccgaat 
      cggggaatg cggccgctt ttggctact ccaaggagt 
      cgaatatcc cgatggggg agcttttga caaaaaata 
      cgtacgttg gcaggacgt tcatccaac cgaacaatc 
      ggcttcgcc agctgaacg tcgccttga agtttagcc 
      ccatatcga cgaatgtaa aaggcaaaa gaattattc 
      ttattgacg attcgattg taagaggca atactattg 
      gtcctatca taaaattgt tacgtcgcg tgggggcta 
      aagaggtgc atattagag tggcaagtc cgcctttga 
      agtacccct gcttcatgg gaattaata ttccgacta 
      gagaagagt tgattgcta atgatttag attcgagaa 
      gtcttgctg ataaagttg gagccgata gtttggaat 
      atttgagtg tggaagggc ttgttaagg cggtacaca 
      gaaatattg aaaaggggg ctgagaagg ggcactgca 
      ccgcttgct tgactggcg aatacccag aagaaatcc 
      cctatgaat tacagtggt aatctcaag gaaaaatat 
      tattgtaat atgatgtaa cattccatt atttttttg 
      gacatgaca agtagtagc aataggatg tgatacaaa 
      ttgcaatca caggtgata caaaatcag tattttgta 
      ttataagaa taaataaac aaacatttt gattgaata 
      ttttatttc taagccaaa agctttgac ctgaaatga 
      atcgcataa taacacaca acaagcgaa aaagcaaca 
      gctaaacat tcacaacta atccacggc cctttgtaa 
      gtccctgga tgattgatt gtgatcact gcaaagtgc 
      ggaactgtt ttcaaatca tctccagct ctctgtgct 
      ttccgcaat aaggcgtaa ccatcgtcg tcaaagagt 
      ttaatagtg atatcccca ttcctggca ttcttcact 
      tcttcagtc caacttatc tggtatttg tttggtccc 
      gtcattcca accggttga attgtatca cccagtaaa 
      gaatacaaa tttgtaagt gatcccgac ccacatttt 
      acattaaat tccgaaatg taagcaatg tgagtgagg 
      attgttgcg tcttgagtt gtgtaactc gtcgactta 
      atttccgga ttttcacac gaactgaac gcgccgctt 
      ataagaaac acttggaac acaaaaaac agcgggatt 
      ttattcatt tttcccgtt atttgaaca aattatgat 
      caccggtag tttacaaat ttgtggtta ggtttaaaa 
      tgtcattaa gtaacctca aaaaatttt aacacgttt 
      ttttggttt tcggttctg caaggatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY