General information | |||
TC000530 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 10141102..10143769 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC000530 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00120 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
abdomen musculature partially irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
some embryos seem to have less abdominal segments. check for cuticle phenotype |
head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
dorsal ridge row partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Comment: Maxilla escort bristles are sometimes partially missing |
abdomen bristle dorsal partiallynot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06133 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC000530 RA
MALKNAPRD DFTSGLTHE CGVFGAIGT SDWPNTNSE IAQIICIGL EALQHRGQE SCGVATSEG SEDFHIHKG MGLVKNVFN NDNISKLKG SLGIGHTRY ATRAASAAI NCQPFVVHS MHGSLATAH NGELVNCSS LRKKVLDRG VGLSTHSDS EIITQALCL IPPEGEVNG PDWPARIRH FMQMAPLSY SLVLMLKDR IYAVRDPYG NRPLCLGKI LPPHCDRND ESVQPEGWL VSSESCAFL TITRYVREV LPGEIVEMT HQGVRTVDI VARPANKQL AFCIFEYVY FARPNSIFE GQEVYTVRS HCGRQLAIE HPVEADIVG SVPESGNAA AFGYSKESN IPMGELLTK NTYVGRTFI QPNNRLRQL NVALKFSPI STNVKGKRI ILIDDSIVR GNTIGPIIK LLRRVGAKE VHIRVASPP LKYPCFMGI NIPTREELI ANDLDSRSL ADKVGADSL EYLSVEGLV KAVHRNIEK GAEKGHCTA CLTGEYPEE IPYELQW
- >TC000530 RA
atggcattg aaaaatgcg cctcgggac gactttact tccggtttg acgcacgaa tgcggcgtt tttggggct atcggcaca agtgactgg cccaacacg aactctgaa atcgctcag ataatctgt atcggtttg gaagccctc cagcatcgg ggtcaagag tcatgcggt gtcgcaaca agcgaagga agcgaagat tttcacatc cacaaaggc atgggcttg gtcaagaac gtcttcaat aacgacaat atcagcaaa ctgaaaggc tcactaggg attgggcac acgagatat gccactagg gccgcttcg gctgctatc aattgccaa ccgtttgtg gtgcactcc atgcacggt tccttggcc acggcgcac aatggcgag ctagtaaac tgctctagt ttgaggaaa aaggttcta gaccggggt gtggggctt tctacgcac tccgacagc gaaattata acacaagct ctgtgctta ataccgcca gaaggcgaa gtcaatggt cccgattgg cccgcaagg atccgtcat ttcatgcaa atggccccc ttgagctac tcgctagtc ctgatgctc aaggaccgc atttacgct gttagggac ccgtacggc aaccggccc ttgtgtctc ggcaaaatt ttgccccct cattgcgac aggaatgac gaaagcgtg cagcctgag ggatggctc gtttcgtcc gaatcgtgc gcctttttg accatcacc agatacgtt cgggaggtt ttaccgggg gaaatcgtc gaaatgaca caccagggt gtgcgaacg gttgatata gtagctcga ccggctaac aaacaacta gctttttgc attttcgaa tatgtttat ttcgcgagg cccaatagt atttttgag gggcaagag gtgtacact gtgcggtcg cactgtggc aggcaattg gccattgaa catccggtt gaagcggac attgtgggg tctgtgccc gaatcgggg aatgcggcc gcttttggc tactccaag gagtcgaat atcccgatg ggggagctt ttgacaaaa aatacgtac gttggcagg acgttcatc caaccgaac aatcggctt cgccagctg aacgtcgcc ttgaagttt agccccata tcgacgaat gtaaaaggc aaaagaatt attcttatt gacgattcg attgtaaga ggcaatact attggtcct atcataaaa ttgttacgt cgcgtgggg gctaaagag gtgcatatt agagtggca agtccgcct ttgaagtac ccctgcttc atgggaatt aatattccg actagagaa gagttgatt gctaatgat ttagattcg agaagtctt gctgataaa gttggagcc gatagtttg gaatatttg agtgtggaa gggcttgtt aaggcggta cacagaaat attgaaaag ggggctgag aaggggcac tgcaccgct tgcttgact ggcgaatac ccagaagaa atcccctat gaattacag tggtaa
- >TC000530 RA
cccattcat gggtctcaa aacgtgcaa caggtgttt tacagttat ttgtaacct gaaaccacg tgtgtgcag tttttgaat ttgtaagtt cagcaatgg cattgaaaa atgcgcctc gggacgact ttacttccg gtttgacgc acgaatgcg gcgtttttg gggctatcg gcacaagtg actggccca acacgaact ctgaaatcg ctcagataa tctgtatcg gtttggaag ccctccagc atcggggtc aagagtcat gcggtgtcg caacaagcg aaggaagcg aagattttc acatccaca aaggcatgg gcttggtca agaacgtct tcaataacg acaatatca gcaaactga aaggctcac tagggattg ggcacacga gatatgcca ctagggccg cttcggctg ctatcaatt gccaaccgt ttgtggtgc actccatgc acggttcct tggccacgg cgcacaatg gcgagctag taaactgct ctagtttga ggaaaaagg ttctagacc ggggtgtgg ggctttcta cgcactccg acagcgaaa ttataacac aagctctgt gcttaatac cgccagaag gcgaagtca atggtcccg attggcccg caaggatcc gtcatttca tgcaaatgg cccccttga gctactcgc tagtcctga tgctcaagg accgcattt acgctgtta gggacccgt acggcaacc ggcccttgt gtctcggca aaattttgc cccctcatt gcgacagga atgacgaaa gcgtgcagc ctgagggat ggctcgttt cgtccgaat cgtgcgcct ttttgacca tcaccagat acgttcggg aggttttac cgggggaaa tcgtcgaaa tgacacacc agggtgtgc gaacggttg atatagtag ctcgaccgg ctaacaaac aactagctt tttgcattt tcgaatatg tttatttcg cgaggccca atagtattt ttgaggggc aagaggtgt acactgtgc ggtcgcact gtggcaggc aattggcca ttgaacatc cggttgaag cggacattg tggggtctg tgcccgaat cggggaatg cggccgctt ttggctact ccaaggagt cgaatatcc cgatggggg agcttttga caaaaaata cgtacgttg gcaggacgt tcatccaac cgaacaatc ggcttcgcc agctgaacg tcgccttga agtttagcc ccatatcga cgaatgtaa aaggcaaaa gaattattc ttattgacg attcgattg taagaggca atactattg gtcctatca taaaattgt tacgtcgcg tgggggcta aagaggtgc atattagag tggcaagtc cgcctttga agtacccct gcttcatgg gaattaata ttccgacta gagaagagt tgattgcta atgatttag attcgagaa gtcttgctg ataaagttg gagccgata gtttggaat atttgagtg tggaagggc ttgttaagg cggtacaca gaaatattg aaaaggggg ctgagaagg ggcactgca ccgcttgct tgactggcg aatacccag aagaaatcc cctatgaat tacagtggt aatctcaag gaaaaatat tattgtaat atgatgtaa cattccatt atttttttg gacatgaca agtagtagc aataggatg tgatacaaa ttgcaatca caggtgata caaaatcag tattttgta ttataagaa taaataaac aaacatttt gattgaata ttttatttc taagccaaa agctttgac ctgaaatga atcgcataa taacacaca acaagcgaa aaagcaaca gctaaacat tcacaacta atccacggc cctttgtaa gtccctgga tgattgatt gtgatcact gcaaagtgc ggaactgtt ttcaaatca tctccagct ctctgtgct ttccgcaat aaggcgtaa ccatcgtcg tcaaagagt ttaatagtg atatcccca ttcctggca ttcttcact tcttcagtc caacttatc tggtatttg tttggtccc gtcattcca accggttga attgtatca cccagtaaa gaatacaaa tttgtaagt gatcccgac ccacatttt acattaaat tccgaaatg taagcaatg tgagtgagg attgttgcg tcttgagtt gtgtaactc gtcgactta atttccgga ttttcacac gaactgaac gcgccgctt ataagaaac acttggaac acaaaaaac agcgggatt ttattcatt tttcccgtt atttgaaca aattatgat caccggtag tttacaaat ttgtggtta ggtttaaaa tgtcattaa gtaacctca aaaaatttt aacacgttt ttttggttt tcggttctg caaggatta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
