Status
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Gene information
General information
TC000530 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10141102..10143769
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000530
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00120

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen musculature partially irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    some embryos seem to have less abdominal segments. check for cuticle phenotype

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    dorsal ridge row partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Maxilla escort bristles are sometimes partially missing
    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen bristle dorsal partiallynot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 100
    Adult morphology
    remarks: later than schedule, hours 100
    iBeetle screen iB_06133

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000530 RA
      MALKNAPRD DFTSGLTHE CGVFGAIGT SDWPNTNSE 
IAQIICIGL EALQHRGQE SCGVATSEG SEDFHIHKG 
MGLVKNVFN NDNISKLKG SLGIGHTRY ATRAASAAI 
NCQPFVVHS MHGSLATAH NGELVNCSS LRKKVLDRG 
VGLSTHSDS EIITQALCL IPPEGEVNG PDWPARIRH 
FMQMAPLSY SLVLMLKDR IYAVRDPYG NRPLCLGKI 
LPPHCDRND ESVQPEGWL VSSESCAFL TITRYVREV 
LPGEIVEMT HQGVRTVDI VARPANKQL AFCIFEYVY 
FARPNSIFE GQEVYTVRS HCGRQLAIE HPVEADIVG 
SVPESGNAA AFGYSKESN IPMGELLTK NTYVGRTFI 
QPNNRLRQL NVALKFSPI STNVKGKRI ILIDDSIVR 
GNTIGPIIK LLRRVGAKE VHIRVASPP LKYPCFMGI 
NIPTREELI ANDLDSRSL ADKVGADSL EYLSVEGLV 
KAVHRNIEK GAEKGHCTA CLTGEYPEE IPYELQW
    CDS sequence
    • >TC000530 RA
      atggcattg aaaaatgcg cctcgggac gactttact 
tccggtttg acgcacgaa tgcggcgtt tttggggct 
atcggcaca agtgactgg cccaacacg aactctgaa 
atcgctcag ataatctgt atcggtttg gaagccctc 
cagcatcgg ggtcaagag tcatgcggt gtcgcaaca 
agcgaagga agcgaagat tttcacatc cacaaaggc 
atgggcttg gtcaagaac gtcttcaat aacgacaat 
atcagcaaa ctgaaaggc tcactaggg attgggcac 
acgagatat gccactagg gccgcttcg gctgctatc 
aattgccaa ccgtttgtg gtgcactcc atgcacggt 
tccttggcc acggcgcac aatggcgag ctagtaaac 
tgctctagt ttgaggaaa aaggttcta gaccggggt 
gtggggctt tctacgcac tccgacagc gaaattata 
acacaagct ctgtgctta ataccgcca gaaggcgaa 
gtcaatggt cccgattgg cccgcaagg atccgtcat 
ttcatgcaa atggccccc ttgagctac tcgctagtc 
ctgatgctc aaggaccgc atttacgct gttagggac 
ccgtacggc aaccggccc ttgtgtctc ggcaaaatt 
ttgccccct cattgcgac aggaatgac gaaagcgtg 
cagcctgag ggatggctc gtttcgtcc gaatcgtgc 
gcctttttg accatcacc agatacgtt cgggaggtt 
ttaccgggg gaaatcgtc gaaatgaca caccagggt 
gtgcgaacg gttgatata gtagctcga ccggctaac 
aaacaacta gctttttgc attttcgaa tatgtttat 
ttcgcgagg cccaatagt atttttgag gggcaagag 
gtgtacact gtgcggtcg cactgtggc aggcaattg 
gccattgaa catccggtt gaagcggac attgtgggg 
tctgtgccc gaatcgggg aatgcggcc gcttttggc 
tactccaag gagtcgaat atcccgatg ggggagctt 
ttgacaaaa aatacgtac gttggcagg acgttcatc 
caaccgaac aatcggctt cgccagctg aacgtcgcc 
ttgaagttt agccccata tcgacgaat gtaaaaggc 
aaaagaatt attcttatt gacgattcg attgtaaga 
ggcaatact attggtcct atcataaaa ttgttacgt 
cgcgtgggg gctaaagag gtgcatatt agagtggca 
agtccgcct ttgaagtac ccctgcttc atgggaatt 
aatattccg actagagaa gagttgatt gctaatgat 
ttagattcg agaagtctt gctgataaa gttggagcc 
gatagtttg gaatatttg agtgtggaa gggcttgtt 
aaggcggta cacagaaat attgaaaag ggggctgag 
aaggggcac tgcaccgct tgcttgact ggcgaatac 
ccagaagaa atcccctat gaattacag tggtaa
    mRNA sequence
    • >TC000530 RA
      cccattcat gggtctcaa aacgtgcaa caggtgttt 
tacagttat ttgtaacct gaaaccacg tgtgtgcag 
tttttgaat ttgtaagtt cagcaatgg cattgaaaa 
atgcgcctc gggacgact ttacttccg gtttgacgc 
acgaatgcg gcgtttttg gggctatcg gcacaagtg 
actggccca acacgaact ctgaaatcg ctcagataa 
tctgtatcg gtttggaag ccctccagc atcggggtc 
aagagtcat gcggtgtcg caacaagcg aaggaagcg 
aagattttc acatccaca aaggcatgg gcttggtca 
agaacgtct tcaataacg acaatatca gcaaactga 
aaggctcac tagggattg ggcacacga gatatgcca 
ctagggccg cttcggctg ctatcaatt gccaaccgt 
ttgtggtgc actccatgc acggttcct tggccacgg 
cgcacaatg gcgagctag taaactgct ctagtttga 
ggaaaaagg ttctagacc ggggtgtgg ggctttcta 
cgcactccg acagcgaaa ttataacac aagctctgt 
gcttaatac cgccagaag gcgaagtca atggtcccg 
attggcccg caaggatcc gtcatttca tgcaaatgg 
cccccttga gctactcgc tagtcctga tgctcaagg 
accgcattt acgctgtta gggacccgt acggcaacc 
ggcccttgt gtctcggca aaattttgc cccctcatt 
gcgacagga atgacgaaa gcgtgcagc ctgagggat 
ggctcgttt cgtccgaat cgtgcgcct ttttgacca 
tcaccagat acgttcggg aggttttac cgggggaaa 
tcgtcgaaa tgacacacc agggtgtgc gaacggttg 
atatagtag ctcgaccgg ctaacaaac aactagctt 
tttgcattt tcgaatatg tttatttcg cgaggccca 
atagtattt ttgaggggc aagaggtgt acactgtgc 
ggtcgcact gtggcaggc aattggcca ttgaacatc 
cggttgaag cggacattg tggggtctg tgcccgaat 
cggggaatg cggccgctt ttggctact ccaaggagt 
cgaatatcc cgatggggg agcttttga caaaaaata 
cgtacgttg gcaggacgt tcatccaac cgaacaatc 
ggcttcgcc agctgaacg tcgccttga agtttagcc 
ccatatcga cgaatgtaa aaggcaaaa gaattattc 
ttattgacg attcgattg taagaggca atactattg 
gtcctatca taaaattgt tacgtcgcg tgggggcta 
aagaggtgc atattagag tggcaagtc cgcctttga 
agtacccct gcttcatgg gaattaata ttccgacta 
gagaagagt tgattgcta atgatttag attcgagaa 
gtcttgctg ataaagttg gagccgata gtttggaat 
atttgagtg tggaagggc ttgttaagg cggtacaca 
gaaatattg aaaaggggg ctgagaagg ggcactgca 
ccgcttgct tgactggcg aatacccag aagaaatcc 
cctatgaat tacagtggt aatctcaag gaaaaatat 
tattgtaat atgatgtaa cattccatt atttttttg 
gacatgaca agtagtagc aataggatg tgatacaaa 
ttgcaatca caggtgata caaaatcag tattttgta 
ttataagaa taaataaac aaacatttt gattgaata 
ttttatttc taagccaaa agctttgac ctgaaatga 
atcgcataa taacacaca acaagcgaa aaagcaaca 
gctaaacat tcacaacta atccacggc cctttgtaa 
gtccctgga tgattgatt gtgatcact gcaaagtgc 
ggaactgtt ttcaaatca tctccagct ctctgtgct 
ttccgcaat aaggcgtaa ccatcgtcg tcaaagagt 
ttaatagtg atatcccca ttcctggca ttcttcact 
tcttcagtc caacttatc tggtatttg tttggtccc 
gtcattcca accggttga attgtatca cccagtaaa 
gaatacaaa tttgtaagt gatcccgac ccacatttt 
acattaaat tccgaaatg taagcaatg tgagtgagg 
attgttgcg tcttgagtt gtgtaactc gtcgactta 
atttccgga ttttcacac gaactgaac gcgccgctt 
ataagaaac acttggaac acaaaaaac agcgggatt 
ttattcatt tttcccgtt atttgaaca aattatgat 
caccggtag tttacaaat ttgtggtta ggtttaaaa 
tgtcattaa gtaacctca aaaaatttt aacacgttt 
ttttggttt tcggttctg caaggatta

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY