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Gene information
General information
TC000529 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 4637527..4642621
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000529
    iBeetle screen iB_00119
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    egg chamber partially fused - Number of animals showing the phenotype: 2

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupa lethality as pupa - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 8

    wings shape irregular - Number of animals showing the phenotype: 2

    elytra blistered - Number of animals showing the phenotype: 3

    elytra necrotic - Number of animals showing the phenotype: 3

    dorsal thoracic muscles potentially differentiation irregular - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000529 RA
      MSRGYGKYR RGGSSHASF HFDNLFKEN ANKPSAARS 
      AATVGKWGI TSFTSIRTS NENEKNASS EGESVPKPK 
      KFFKSRNTE PPDLSSSYK ADMTYGNVT KKLKLVNEP 
      TEKSKSVGR KFFSSKSNS PEKTVLSSN VNNSKRNET 
      KPPIVLRIC KGKSQLVND SDESESTPT PSSTPSTSN 
      ATSPRALRD TAQRSPNCR ITRSARRSM QQDPSSSPA 
      TADTPTEFS LFTSPKQDI DLSPQYIPA ERYEMERKA 
      MYDNLLKPN SPSQLFPEE KNSTEDSGI DYVDSEISA 
      EKEPEETDK TEQIETKPS ETIEEKSQP VEETKSGEE 
      PVTKGSIEE EWDTDEDSN STTPDSEIK TQLTEDSEP 
      LPLPVEEQK EKSAEEGAP ETSPPVKLV ISKKKGSIF 
      KSRPLVTDV TKKRRALYR HKWSDDKDA TQKSNENPS 
      SNNTGGSVY DEFSFNDDP LTRVTVGGN SDGEDESVT 
      SIKCTKSDK GFYTVVRNV KKAHQIQEI GEFQEFNDD 
      VEYILDALQ DNNPISTRC LSAITLASK CMVPAFRMH 
      VRAHGTVAK FFKALHDAT KDQSLGLCT ATVMFVLSQ 
      DRLNMDLDR DCLELMLNL LESDVSYQQ ALDVCGLSS 
      AQLEKNKQK VRELCAEIQ SQGHAMHLN LDNITVGQL 
      AMETLLSLT SKRAGEWFK EELRELGGL EHIMKTICE 
      CCRQVSDYV VSWTDALLD KLRKVDRCL RVLENVTHN 
      NEENQTYLL KYNDGVILD TLVNLYKLC DGEIPLYPV 
      TDITDKEST GTIIREALL VTLKVLINL THHFSKQSM 
      GSTLIGSRN GIIETSLHL LLQVPHYIP EQKKFELGV 
      LVLMLLINL IQDNDNNKK LLIEAKAPS KLESIYSRE 
      ESAVEALIA QFYHWEGCA KIAEKRTNA ILDGEKDEN 
      SGNVAYKSN EEFIEETVA KLLQKAGTH MEYTFLASY 
      IVMLIGFLI MDNKEYQNN VRQFLRGNN FADMVELLK 
      KFFNFMNLT ASTEASSVC AIKATEKVI KFLEECDSP 
      PPEEKPSYT ECMDLSYTM AH
    CDS sequence
    • >TC000529 RA
      atgtcaaga gggtatggg aagtatcgc cgtggagga 
      agttcacat gcaagtttc catttcgac aatttattt 
      aaggaaaat gctaacaaa ccatctgcc gcaaggtct 
      gcagccact gtgggcaag tggggcatc acctccttc 
      acttccata cgtaccagc aatgaaaac gaaaaaaac 
      gcctcatct gaaggagag tccgtgccg aagccgaag 
      aagttcttc aagagtcgc aacacggaa ccccctgac 
      ctgtcttcg tcctacaag gccgacatg acatacggc 
      aacgtaaca aagaagctg aaattagta aacgaaccg 
      acggagaaa tccaaaagt gttggtcgt aaatttttt 
      tcgtcaaag agcaactcg cctgaaaag actgtacta 
      agttcaaat gtaaataat tctaaacga aacgaaaca 
      aaacctcca atagtttta aggatatgt aaaggaaaa 
      tcccagtta gtgaacgat tcggacgaa tctgaaagc 
      acgcccaca ccatccagc accccatcg acttcaaac 
      gccacctct ccaagggct ctcagggac acagcacaa 
      agatcacca aattgtcgc atcaccagg tcagcgaga 
      agaagcatg caacaggac cccagcagc agtccggca 
      acggccgac actccgacc gaattctcc ctcttcact 
      tcgcccaaa caggacata gacctgtcc ccgcaatac 
      atcccagct gaaagatac gaaatggag cggaaagcc 
      atgtacgat aacttgctt aaaccaaac tcgccttcg 
      cagctgttc cccgaagag aagaacagc accgaagat 
      tcaggaatt gattacgta gatagcgaa attagtgcc 
      gaaaaagag ccggaagaa accgacaaa accgaacaa 
      atcgaaact aaaccgagt gaaaccatc gaagaaaaa 
      agccaaccg gttgaagaa accaaatcg ggggaagaa 
      ccagtgacc aagggttca atagaagag gaatgggac 
      accgacgag gacagcaac agcaccacc ccagactca 
      gaaatcaaa acgcagcta accgaggat tcggaaccg 
      ctcccactg ccggtggaa gagcagaag gagaaaagt 
      gcggaagaa ggcgccccc gaaactagc cctcccgtt 
      aaattagtt atctcaaag aagaaagga agtattttt 
      aaaagtaga cctttagtt acggacgtg acgaaaaaa 
      cggcgcgcg ttgtacaga cacaagtgg agtgatgat 
      aaagacgcc acgcaaaaa tcgaacgaa aatcctagt 
      agcaataat acgggtggt tcagtgtat gacgagttc 
      agttttaat gatgatcct ttgacgagg gtcacggtt 
      gggggcaac agcgacggc gaggacgag agtgtgacc 
      agtatcaag tgcaccaag agtgacaaa gggttttac 
      acagtcgtc cgcaacgtg aagaaagcc caccagatc 
      caggaaatc ggcgaattt caagaattc aacgacgac 
      gtggagtac atccttgat gccttgcag gataataat 
      ccaatcagt actcgctgt ttatcggcc attactcta 
      gcttctaaa tgtatggtg cctgcgttc cggatgcac 
      gtgcgtgct cacggaacc gtagctaaa tttttcaag 
      gcactacat gacgccacc aaagaccaa agtcttggt 
      ctatgtaca gcgactgtc atgttcgtc ctgagccag 
      gaccggctc aacatggac ctagaccgc gattgcctc 
      gaactcatg ttaaacctt ctcgaatcg gacgtaagc 
      taccagcaa gcgctcgac gtctgcggc ttgagctcg 
      gcccagttg gagaaaaat aaacagaaa gttagggag 
      ctgtgtgcc gagatacaa agtcaaggg catgccatg 
      catctgaat ctcgataat ataactgtg ggtcaattg 
      gcaatggag acgcttttg agtctcacg tcgaaaagg 
      gcaggcgag tggttcaag gaggagctg cgggagttg 
      ggcggcttg gagcatatt atgaagacg atatgcgag 
      tgctgtaga caggtgtct gattatgtg gtttcgtgg 
      actgatgcc ttgttagat aaacttcgg aaagttgat 
      aggtgttta cgtgtgctg gaaaatgtc actcataat 
      aatgaagag aatcaaacg tacctgtta aaatataat 
      gatggagta attttagat acgctcgtt aatttgtat 
      aaactatgt gatggtgaa atacctctg tatccagtg 
      actgatata accgataag gagtcgact gggactata 
      ataagggaa gctcttctc gtaactctg aaagtatta 
      attaattta acacaccac tttagtaaa caatccatg 
      ggaagtacc ttaataggt tcgaggaac ggcattatt 
      gaaacaagc ctgcatctg cttctccaa gtgccgcac 
      tatatcccc gaacaaaag aaattcgaa ttgggagtt 
      ttggtcctc atgttgttg attaacctt attcaagat 
      aacgacaac aacaagaag ctgttgatt gaagccaaa 
      gccccgtca aagctggaa agtatttat tcaagagaa 
      gaaagtgcc gttgaggct ttgatcgcg caattttat 
      cactgggaa gggtgtgcg aaaatcgct gaaaaacgg 
      accaacgcg atattggac ggtgaaaaa gatgagaac 
      agtggtaac gttgcgtac aagtcaaat gaggaattt 
      atcgaggaa actgtcgct aaattgcta caaaaagcc 
      ggcacacat atggagtac accttcctg gcttcgtac 
      atcgttatg ttgattgga ttcctgata atggacaac 
      aaggagtac caaaataac gtgcgacag ttcctccgt 
      gggaacaat tttgccgat atggtcgaa ctgttgaag 
      aagttcttc aattttatg aatttaact gcttcgacg 
      gaagcgtca agcgtttgc gcaattaaa gccaccgaa 
      aaagtcatc aagtttctg gaagagtgt gacagtcct 
      ccgcctgaa gagaagccg agttatacg gagtgtatg 
      gatcttagt tacacaatg gcacactag
    mRNA sequence
    • >TC000529 RA
      tttataacg tgaaaattc tattgacaa gaaaaacct 
      aattatttc ccgcatttt cccaaaagt ggaaaccct 
      catacaaat aatgtggta tctgtgaca ccttaccaa 
      tgcctgcaa gcccactta ataaatatc aatggacct 
      tcatcattt gatgaaaat ggcgaagtt gaaccaatt 
      ttaacaaaa tacaccaaa atgtacaga taaatcgcc 
      aaaataaca tcatggaac tcgatagta ttactagat 
      aggacgttg gggacgcgt ttgtattgc caggaattt 
      tgccagggc tcaagtttt aatccgaaa tgtttgagt 
      gaggggtac ttttgagtt caactttta aaaatgtca 
      agagggtat gggaagtat cgccgtgga ggaagttca 
      catgcaagt ttccatttc gacaattta tttaaggaa 
      aatgctaac aaaccatct gccgcaagg tctgcagcc 
      actgtgggc aagtggggc atcacctcc ttcacttcc 
      atacgtacc agcaatgaa aacgaaaaa aacgcctca 
      tctgaagga gagtccgtg ccgaagccg aagaagttc 
      ttcaagagt cgcaacacg gaaccccct gacctgtct 
      tcgtcctac aaggccgac atgacatac ggcaacgta 
      acaaagaag ctgaaatta gtaaacgaa ccgacggag 
      aaatccaaa agtgttggt cgtaaattt ttttcgtca 
      aagagcaac tcgcctgaa aagactgta ctaagttca 
      aatgtaaat aattctaaa cgaaacgaa acaaaacct 
      ccaatagtt ttaaggata tgtaaagga aaatcccag 
      ttagtgaac gattcggac gaatctgaa agcacgccc 
      acaccatcc agcacccca tcgacttca aacgccacc 
      tctccaagg gctctcagg gacacagca caaagatca 
      ccaaattgt cgcatcacc aggtcagcg agaagaagc 
      atgcaacag gaccccagc agcagtccg gcaacggcc 
      gacactccg accgaattc tccctcttc acttcgccc 
      aaacaggac atagacctg tccccgcaa tacatccca 
      gctgaaaga tacgaaatg gagcggaaa gccatgtac 
      gataacttg cttaaacca aactcgcct tcgcagctg 
      ttccccgaa gagaagaac agcaccgaa gattcagga 
      attgattac gtagatagc gaaattagt gccgaaaaa 
      gagccggaa gaaaccgac aaaaccgaa caaatcgaa 
      actaaaccg agtgaaacc atcgaagaa aaaagccaa 
      ccggttgaa gaaaccaaa tcgggggaa gaaccagtg 
      accaagggt tcaatagaa gaggaatgg gacaccgac 
      gaggacagc aacagcacc accccagac tcagaaatc 
      aaaacgcag ctaaccgag gattcggaa ccgctccca 
      ctgccggtg gaagagcag aaggagaaa agtgcggaa 
      gaaggcgcc cccgaaact agccctccc gttaaatta 
      gttatctca aagaagaaa ggaagtatt tttaaaagt 
      agaccttta gttacggac gtgacgaaa aaacggcgc 
      gcgttgtac agacacaag tggagtgat gataaagac 
      gccacgcaa aaatcgaac gaaaatcct agtagcaat 
      aatacgggt ggttcagtg tatgacgag ttcagtttt 
      aatgatgat cctttgacg agggtcacg gttgggggc 
      aacagcgac ggcgaggac gagagtgtg accagtatc 
      aagtgcacc aagagtgac aaagggttt tacacagtc 
      gtccgcaac gtgaagaaa gcccaccag atccaggaa 
      atcggcgaa tttcaagaa ttcaacgac gacgtggag 
      tacatcctt gatgccttg caggataat aatccaatc 
      agtactcgc tgtttatcg gccattact ctagcttct 
      aaatgtatg gtgcctgcg ttccggatg cacgtgcgt 
      gctcacgga accgtagct aaatttttc aaggcacta 
      catgacgcc accaaagac caaagtctt ggtctatgt 
      acagcgact gtcatgttc gtcctgagc caggaccgg 
      ctcaacatg gacctagac cgcgattgc ctcgaactc 
      atgttaaac cttctcgaa tcggacgta agctaccag 
      caagcgctc gacgtctgc ggcttgagc tcggcccag 
      ttggagaaa aataaacag aaagttagg gagctgtgt 
      gccgagata caaagtcaa gggcatgcc atgcatctg 
      aatctcgat aatataact gtgggtcaa ttggcaatg 
      gagacgctt ttgagtctc acgtcgaaa agggcaggc 
      gagtggttc aaggaggag ctgcgggag ttgggcggc 
      ttggagcat attatgaag acgatatgc gagtgctgt 
      agacaggtg tctgattat gtggtttcg tggactgat 
      gccttgtta gataaactt cggaaagtt gataggtgt 
      ttacgtgtg ctggaaaat gtcactcat aataatgaa 
      gagaatcaa acgtacctg ttaaaatat aatgatgga 
      gtaatttta gatacgctc gttaatttg tataaacta 
      tgtgatggt gaaatacct ctgtatcca gtgactgat 
      ataaccgat aaggagtcg actgggact ataataagg 
      gaagctctt ctcgtaact ctgaaagta ttaattaat 
      ttaacacac cactttagt aaacaatcc atgggaagt 
      accttaata ggttcgagg aacggcatt attgaaaca 
      agcctgcat ctgcttctc caagtgccg cactatatc 
      cccgaacaa aagaaattc gaattggga gttttggtc 
      ctcatgttg ttgattaac cttattcaa gataacgac 
      aacaacaag aagctgttg attgaagcc aaagccccg 
      tcaaagctg gaaagtatt tattcaaga gaagaaagt 
      gccgttgag gctttgatc gcgcaattt tatcactgg 
      gaagggtgt gcgaaaatc gctgaaaaa cggaccaac 
      gcgatattg gacggtgaa aaagatgag aacagtggt 
      aacgttgcg tacaagtca aatgaggaa tttatcgag 
      gaaactgtc gctaaattg ctacaaaaa gccggcaca 
      catatggag tacaccttc ctggcttcg tacatcgtt 
      atgttgatt ggattcctg ataatggac aacaaggag 
      taccaaaat aacgtgcga cagttcctc cgtgggaac 
      aattttgcc gatatggtc gaactgttg aagaagttc 
      ttcaatttt atgaattta actgcttcg acggaagcg 
      tcaagcgtt tgcgcaatt aaagccacc gaaaaagtc 
      atcaagttt ctggaagag tgtgacagt cctccgcct 
      gaagagaag ccgagttat acggagtgt atggatctt 
      agttacaca atggcacac tagcattta aatattgta 
      aagaatcag ttttggtaa atcgtaaga acgtttcaa 
      agtggtgat tgactatga aaatgcacc atcatttct 
      ctagtactt acagtttac tgaaatcac aaaagtaga 
      aaaaataca aaaaaatag tcatattga tagttgacc 
      tagattact gaactgaaa catttcatt ctttatttt 
      attacctta ttctgttgt tagtattat ttagtgtta 
      agcctatga aatttaacg taaggttca gtatgtcgt 
      tttatattt ttatcgtgg cgaatttat agttttgtt 
      caagtgtat atacttact actgtatat gaattttct 
      tatttattt catgactag attacttgt aaaattacc 
      ataatgtta tttggattg tttaaatat aatgtacat 
      aataaa