Status
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Gene information
General information
TC000526 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10182303..10184439
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC000526
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_00118

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      remarks: later than schedule, hours 100
      Adult morphology
      remarks: later than schedule, hours 100
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC000526 RA
        MGEDVELCL EIQVDSETK RILSNPHKC RSTLLDVTV 
        IKPKELEAF GTQRTNTLR ADEVRKLIT SMEKQRRKK 
        QKVLEKLKC DDTVLQIRK HDDADAKKE FAKNKERIS 
        SNSPVDDLE RKDFIGIAE SVEPLFCEI RRSLKNVET 
        RITNMQDIN LVISQTTVD ETASEEFVI SRHKKPSSL 
        PSSLSNRNH DCYRIETAP HDIRSILLP QKKQKLLDK 
        DDKVEGKCL CENCGIIGL LAECQKHPL LVELSESPS 
        PSSHKQFPS PRKKPKINL ENISKMRTK GNGDSYINH 
        LSNRIKVLE ERLAMQEEK VVPKDYFKK IISKMVTGL 
        SPKIVKSKS DTSLRSPLK NYKKCSKLQ DIQERPTEK 
        SLQCQSSEA LHCFRSFKE DKKPALKDD KGTITDEEL 
        FYGGYIWKW GEEILKPGI DLKNRIVNL LNDILGKFA 
        LSERGSIEA AQAESIREE AIRKIMRHM NAGNYDPRE 
        NIRHLSPRE KSPKSNPQR VKSPYSKSD HKHNKNVDV 
        SYINPKISL WRKDSPDVS QQKSNTSRK VSFWDRPSP 
        EKYKVSQRK SSAQSPQWR RSSPEKFRT SQRMLEQRN 
        NEQGKHEFL KIVSKAKQH QKDILWKNI WNQAKANGQ 
        TKNDKITIK IPVQSKGNK SEHIFETEI SIGEIEQIL 
        KGVISLC
      CDS sequence
      • >TC000526 RA
        atgggagag gatgtggaa ctctgttta gaaatccag 
        gtggattca gaaactaaa cgaatatta tctaatccc 
        cacaaatgt cgttccact ttgctagac gttactgta 
        atcaagcca aaagagctg gaagcgttt ggtacacaa 
        agaaccaat acgcttcga gctgacgaa gtcagaaaa 
        ttaatcacg tcgatggaa aagcagaga agaaaaaaa 
        cagaaagtt cttgaaaaa ttaaagtgt gacgatact 
        gttctccaa attcgcaaa cacgacgat gctgatgcg 
        aaaaaagaa ttcgccaaa aataaagaa cgaatttct 
        tccaattct ccagttgat gacttagaa cgtaaagac 
        tttatcggg attgctgag tcggtagaa cctttattt 
        tgcgaaatt cgtcgttcg ttgaaaaac gtggaaaca 
        agaataact aacatgcaa gacatcaat cttgttatc 
        agtcaaacc acagtcgac gaaactgct tcagaagaa 
        tttgtgatt tcgagacac aaaaagccc agttctttg 
        cccagttct ctcagtaat cggaaccat gattgctac 
        agaattgaa acagcaccg catgacata agatcaata 
        cttttacct caaaagaaa caaaaattg ttagataaa 
        gacgacaag gtcgaaggg aaatgtctg tgtgaaaac 
        tgcggtatt ataggattg cttgctgaa tgtcaaaag 
        catccactt cttgtcgaa cttagtgaa tctccatca 
        ccttcgtct cataaacag ttcccgagt ccaagaaaa 
        aaaccaaag ataaatctc gaaaatatc agtaaaatg 
        aggacaaag ggtaatggt gattcctac ataaaccat 
        ctttcgaac aggattaaa gtcctggag gagagacta 
        gcaatgcag gaggaaaaa gttgttcca aaagattac 
        ttcaaaaaa atcatctcc aagatggtc actggccta 
        tcccccaaa atcgtcaaa tctaaaagt gacacttct 
        ttgcgttcc ccacttaaa aattacaag aaatgttca 
        aagttgcaa gatatacag gaacgtccg actgaaaaa 
        tctctacaa tgtcaaagc tcggaagct ttgcattgt 
        ttccgcagt ttcaaagaa gataaaaaa ccagccttg 
        aaagacgac aaaggcaca atcaccgac gaggaactg 
        ttttacggc ggatacatt tggaaatgg ggcgaggag 
        attttaaaa ccaggaatt gatttgaaa aatagaatc 
        gtaaattta ctcaacgac attttgggc aagttcgct 
        ttgtccgag agagggtca atagaggcg gcgcaagcc 
        gaaagcata agagaagaa gctataaga aaaatcatg 
        cgacacatg aatgcgggt aattacgat cctcgtgaa 
        aatatccgt catttaagt ccacgcgaa aaaagtccc 
        aaaagtaat ccacaacgg gtcaagtct ccttactct 
        aaaagcgac cacaaacac aataaaaac gtcgatgtt 
        tcctacata aacccaaaa atctccctt tggagaaaa 
        gactctcca gacgtgtcg caacagaag agtaacaca 
        agtcggaaa gtgtctttt tgggacagg ccttcgcca 
        gaaaaatat aaagtatcg cagaggaaa agtagcgca 
        caatcgcct cagtggcgt aggtcctct ccagagaaa 
        tttaggacg agtcaaaga atgttggag cagagaaat 
        aacgaacaa ggcaaacat gaatttttg aaaattgtt 
        agtaaagca aagcagcat cagaaagac attttgtgg 
        aagaatata tggaaccag gccaaagcc aacggccag 
        acgaaaaac gacaaaata acaataaaa ataccagtg 
        cagagcaag ggaaataag agcgagcac atctttgaa 
        accgagata agtatcggc gagatcgag caaattctg 
        aaaggtgtt atctccctg tgttaa
      mRNA sequence
      • >TC000526 RA
        acaaatttc attacaaaa tttatgaaa tatgtaaca 
        atgaaaatt cctacgttt ttacgtgtt gttaagtgt 
        actacgttt tcgtgtctt gtgcaacat gggagagga 
        tgtggaact ctgtttaga aatccaggt ggattcaga 
        aactaaacg aatattatc taatcccca caaatgtcg 
        ttccacttt gctagacgt tactgtaat caagccaaa 
        agagctgga agcgtttgg tacacaaag aaccaatac 
        gcttcgagc tgacgaagt cagaaaatt aatcacgtc 
        gatggaaaa gcagagaag aaaaaaaca gaaagttct 
        tgaaaaatt aaagtgtga cgatactgt tctccaaat 
        tcgcaaaca cgacgatgc tgatgcgaa aaaagaatt 
        cgccaaaaa taaagaacg aatttcttc caattctcc 
        agttgatga cttagaacg taaagactt tatcgggat 
        tgctgagtc ggtagaacc tttattttg cgaaattcg 
        tcgttcgtt gaaaaacgt ggaaacaag aataactaa 
        catgcaaga catcaatct tgttatcag tcaaaccac 
        agtcgacga aactgcttc agaagaatt tgtgatttc 
        gagacacaa aaagcccag ttctttgcc cagttctct 
        cagtaatcg gaaccatga ttgctacag aattgaaac 
        agcaccgca tgacataag atcaatact tttacctca 
        aaagaaaca aaaattgtt agataaaga cgacaaggt 
        cgaagggaa atgtctgtg tgaaaactg cggtattat 
        aggattgct tgctgaatg tcaaaagca tccacttct 
        tgtcgaact tagtgaatc tccatcacc ttcgtctca 
        taaacagtt cccgagtcc aagaaaaaa accaaagat 
        aaatctcga aaatatcag taaaatgag gacaaaggg 
        taatggtga ttcctacat aaaccatct ttcgaacag 
        gattaaagt cctggagga gagactagc aatgcagga 
        ggaaaaagt tgttccaaa agattactt caaaaaaat 
        catctccaa gatggtcac tggcctatc ccccaaaat 
        cgtcaaatc taaaagtga cacttcttt gcgttcccc 
        acttaaaaa ttacaagaa atgttcaaa gttgcaaga 
        tatacagga acgtccgac tgaaaaatc tctacaatg 
        tcaaagctc ggaagcttt gcattgttt ccgcagttt 
        caaagaaga taaaaaacc agccttgaa agacgacaa 
        aggcacaat caccgacga ggaactgtt ttacggcgg 
        atacatttg gaaatgggg cgaggagat tttaaaacc 
        aggaattga tttgaaaaa tagaatcgt aaatttact 
        caacgacat tttgggcaa gttcgcttt gtccgagag 
        agggtcaat agaggcggc gcaagccga aagcataag 
        agaagaagc tataagaaa aatcatgcg acacatgaa 
        tgcgggtaa ttacgatcc tcgtgaaaa tatccgtca 
        tttaagtcc acgcgaaaa aagtcccaa aagtaatcc 
        acaacgggt caagtctcc ttactctaa aagcgacca 
        caaacacaa taaaaacgt cgatgtttc ctacataaa 
        cccaaaaat ctccctttg gagaaaaga ctctccaga 
        cgtgtcgca acagaagag taacacaag tcggaaagt 
        gtctttttg ggacaggcc ttcgccaga aaaatataa 
        agtatcgca gaggaaaag tagcgcaca atcgcctca 
        gtggcgtag gtcctctcc agagaaatt taggacgag 
        tcaaagaat gttggagca gagaaataa cgaacaagg 
        caaacatga atttttgaa aattgttag taaagcaaa 
        gcagcatca gaaagacat tttgtggaa gaatatatg 
        gaaccaggc caaagccaa cggccagac gaaaaacga 
        caaaataac aataaaaat accagtgca gagcaaggg 
        aaataagag cgagcacat ctttgaaac cgagataag 
        tatcggcga gatcgagca aattctgaa aggtgttat 
        ctccctgtg ttaagaata aaaaaaggt ttggttt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY