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Gene information
General information
TC000515 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10315884..10316788
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC000515
      iBeetle screen iB_00113

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      remarks: later than schedule, hours 100
      Adult morphology
      remarks: later than schedule, hours 100
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC000515 RA
        MFAFALLGL IAAAQAVEF EIVNNEGGP 
        VWVGIQGNP GHEHLSNGG LILNQGQSV 
        TLQAAEDWA GRFWPRTWC DPDTQHCQT 
        GDCGNKVEC GGAGGVPPA SLAEITLKG 
        YGGIDYYDI SLVDGYNIR IAMEPIGGH 
        GDGSQYSCT KCACSVNLL DICPQELKI 
        INGEGYTIA CNSACGAFN TDEYCCRGA 
        HNTPETCKS SDWPVDYPA FFKSNCPDA 
        YSYAYDDHK STFTCQAEK YRVTFG
      CDS sequence
      • >TC000515 RA
        atgttcgca ttcgctctt cttggtctc atcgctgcg 
        gctcaggcc gtcgaattt gaaatagtc aacaacgaa 
        ggtggccct gtttgggtg ggcattcag ggaaaccca 
        ggacatgaa cacctcagc aatggtgga ttgattttg 
        aatcagggc caaagtgtc actttgcaa gctgcagaa 
        gactgggct ggtcgtttc tggcccagg acttggtgc 
        gaccccgac acccagcat tgccagacc ggagactgt 
        ggaaacaaa gtcgaatgt gggggtgct ggcggtgtt 
        cctccagct tccttggcc gaaatcacc cttaaaggg 
        tatggaggt atcgactat tatgatatt tctttggtc 
        gatggctac aacatccga attgccatg gaaccaatt 
        gggggacat ggggatggt agccaatac agttgcacc 
        aaatgtgcc tgcagtgta aatctcctc gacatttgc 
        ccacaagaa ctcaaaatc atcaatggt gaaggttac 
        accattgct tgtaactcc gcttgtggt gctttcaac 
        accgatgag tactgttgc cgtggcgct cacaacacg 
        cctgaaacc tgcaagtcg tccgattgg cccgtcgat 
        tatccagct ttcttcaaa agcaattgt cctgacgct 
        tacagctac gcttacgat gaccataag agtaccttc 
        acttgccaa gcagaaaaa taccgtgtt accttcggt 
        taa
      mRNA sequence
      • >TC000515 RA
        agttccaca atgttcgca ttcgctctt cttggtctc 
        atcgctgcg gctcaggcc gtcgaattt gaaatagtc 
        aacaacgaa ggtggccct gtttgggtg ggcattcag 
        ggaaaccca ggacatgaa cacctcagc aatggtgga 
        ttgattttg aatcagggc caaagtgtc actttgcaa 
        gctgcagaa gactgggct ggtcgtttc tggcccagg 
        acttggtgc gaccccgac acccagcat tgccagacc 
        ggagactgt ggaaacaaa gtcgaatgt gggggtgct 
        ggcggtgtt cctccagct tccttggcc gaaatcacc 
        cttaaaggg tatggaggt atcgactat tatgatatt 
        tctttggtc gatggctac aacatccga attgccatg 
        gaaccaatt gggggacat ggggatggt agccaatac 
        agttgcacc aaatgtgcc tgcagtgta aatctcctc 
        gacatttgc ccacaagaa ctcaaaatc atcaatggt 
        gaaggttac accattgct tgtaactcc gcttgtggt 
        gctttcaac accgatgag tactgttgc cgtggcgct 
        cacaacacg cctgaaacc tgcaagtcg tccgattgg 
        cccgtcgat tatccagct ttcttcaaa agcaattgt 
        cctgacgct tacagctac gcttacgat gaccataag 
        agtaccttc acttgccaa gcagaaaaa taccgtgtt 
        accttcggt taagcgttt tatatttac tcatttatt 
        taataataa aacattttc tcagtaaaa atcgtatct 
        cttattttt cttaatcct ttatttacg ttctcacga 
        aacataaac aataaatgt tgcaaggtg ag
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY