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Gene information
General information
TC000511 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 4923913..4928605
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000511
    iBeetle screen iB_00111
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000511 RA
      MAFIRVIAL ALALASTTA WSSHPFESA RDQYEARED 
      RPQRQLLTE ATDHRPGVH MSQQSDFIL PVQRKSVRK 
      RSYYPWQNE VTYEVPYQS ITVWKSPVY QLSRPYYIP 
      IFGVPGRVP IYYPPQPFV NPGVPKDNT GKKPFVAPT 
      YLPPVETTT MTIRDRFNG GDNYDDRPI WADETGKTT 
      QRPGTGAVP TRKPRPSRP RTPPPLVHN PTDPEGFAK 
      ESEDSNQPS TTVSTPQFR PNEPSRCVW AIISCCSST 
      SEVSYDCFE QRGCPGAFW DKSPCDNEF AQAAIEAAL 
      NYYNKK
    CDS sequence
    • >TC000511 RA
      atggcattt atccgcgtg atagcattg gcgttagcg 
      ctggcaagc actacagca tggagttct catcccttc 
      gaatcggca cgagatcaa tacgaagcg cgcgaggat 
      agaccccaa cgccagctg ctcaccgaa gcaacagat 
      catcgtcca ggagtccac atgtctcaa caatccgac 
      ttcatcctt ccagtccaa aggaaaagt gtgcgaaaa 
      cgaagttac tatccttgg caaaacgaa gtgacttac 
      gaagtgcca tatcagagc atcactgtt tggaagtct 
      ccagtctac caactgagc aggccgtac tatatcccc 
      attttcggg gttccgggg cgagtccct atttactac 
      ccgccacaa cctttcgtt aatcccgga gtaccgaag 
      gataatacg ggcaaaaag ccatttgtg gcgcccacg 
      tacttgcca ccggtcgaa accacaacc atgactatc 
      agagacagg tttaacggt ggtgataac tacgacgac 
      aggcccata tgggccgat gagaccgga aaaacgacc 
      caaaggccg gggactggc gcggttcca acccggaaa 
      ccacgacca tcaaggccc aggacgccc ccgcctctt 
      gtccacaac cccactgat cctgaagga ttcgcgaag 
      gaatcggaa gattcaaat caaccgtca actaccgtg 
      tcaacgcct caatttagg cccaatgaa cccagcaga 
      tgcgtctgg gcgataatt tcctgctgc tcgtccacg 
      tctgaagtc agttatgac tgcttcgaa caacgaggg 
      tgtcctgga gcgttctgg gacaagagt ccgtgtgac 
      aacgagttt gcccaagcg gccatcgag gccgcgctt 
      aattattac aataagaaa taa
    mRNA sequence
    • >TC000511 RA
      agcaacaac tccaacatt gcggtttac cacataata 
      acgttacgt tagtttgag tgttgaaga atttagaag 
      acatataat taattattc gagaccact gacagttgt 
      tgcaggatg gcatttatc cgcgtgata gcattggcg 
      ttagcgctg gcaagcact acagcatgg agttctcat 
      cccttcgaa tcggcacga gatcaatac gaagcgcgc 
      gaggataga ccccaacgc cagctgctc accgaagca 
      acagatcat cgtccagga gtccacatg tctcaacaa 
      tccgacttc atccttcca gtccaaagg aaaagtgtg 
      cgaaaacga agttactat ccttggcaa aacgaagtg 
      acttacgaa gtgccatat cagagcatc actgtttgg 
      aagtctcca gtctaccaa ctgagcagg ccgtactat 
      atccccatt ttcggggtt ccggggcga gtccctatt 
      tactacccg ccacaacct ttcgttaat cccggagta 
      ccgaaggat aatacgggc aaaaagcca tttgtggcg 
      cccacgtac ttgccaccg gtcgaaacc acaaccatg 
      actatcaga gacaggttt aacggtggt gataactac 
      gacgacagg cccatatgg gccgatgag accggaaaa 
      acgacccaa aggccgggg actggcgcg gttccaacc 
      cggaaacca cgaccatca aggcccagg acgcccccg 
      cctcttgtc cacaacccc actgatcct gaaggattc 
      gcgaaggaa tcggaagat tcaaatcaa ccgtcaact 
      accgtgtca acgcctcaa tttaggccc aatgaaccc 
      agcagatgc gtctgggcg ataatttcc tgctgctcg 
      tccacgtct gaagtcagt tatgactgc ttcgaacaa 
      cgagggtgt cctggagcg ttctgggac aagagtccg 
      tgtgacaac gagtttgcc caagcggcc atcgaggcc 
      gcgcttaat tattacaat aagaaataa agttcctaa 
      aattgaact aaccgtgtg ttaactgca tgaatttaa 
      cggtcagta ttaaaaaag tgtgtttgg ggatgtttt 
      cgagtgttg acacttgag caagacgga tggttatta 
      taagtatgc agcgtccaa ttaaaagga aaataaaac 
      tccgtgtta gttgtagct gaaagaggc aacagagtg 
      gagagctct gagctacca ccatctgtc gcagttcct 
      gacaacagc cgcagtggt aattaaatt cgccgaatg 
      tcgactgaa tgttgaacc ggggtttta tttgaaaag 
      atgttcgat gcgccaaat caggaaatc atgccaatt 
      tgtctcgga gaataattg aaccaaaaa tgcttttcg 
      tagacaatt ttattgtat tagggttat ttatttttt 
      attaaaacg aaaataaag cttagtgtc tact