Status
Please wait ...
Gene information
General information
TC000504 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10518072..10521166
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000504
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00110

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    vitellarium not present - Number of animals showing the phenotype: 4

    tropharium size decreased - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 100
    Adult morphology
    abdominal odoriferous glands size increased - Number of animals showing the phenotype: 4 (41 dpi)
    Days post injection



    remarks: later than schedule, hours 100
    Fertility
    egg production: number no/almost no eggs -
    ovariole size decreased - Number of animals showing the phenotype: 4

    tropharium not present - Number of animals showing the phenotype: 4
    vitellarium not present - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000504 RA
      MSSSNKKKG KNPAAVDNW LDSIGLYRK NVAYDETCL 
      FRAVSEQLF QCQIYHERV RKECIDYGR RHYYDFWHL 
      LEDENEWYN HLDLLEKHM VVCGNIEIQ LISHKYNRD 
      VVVYDATHQ SVLQVTRRG LDKTLMLCL MNEDHYDVV 
      YEKEHIQNA GFCQSIVYG ILYENVFDV PNVQNIVHG 
      MLYEKEFVA LSFADVSDN FESKLSLEK EGDKNTEVV 
      VPPPVNIAP FPFKVAKAL DPTIYRNIE YDSWTEVRR 
      ELRLGDWYY GDDNLILGT RCILNQDDQ TYECYIQEI 
      IKNENKCVV YVTKLAEKR TVNYTDLSP ENDAKPWPL 
      PYRFTKNQV ATPSQAVTL ETKLKLPKK RKDKRRVKS 
      NSESQTQAV SSQQLAQDI EAFIGQPLQ MQNNSGQNY 
      NQAQSPPEI SSSQSQHNL LVTPDIPSP MPKFSWETP 
      WSTAAAASC EKFVWSQPA TSPGMKPMV ASAPTTPNV 
      IPYHDSQYP FVYNYPVDS SFNSWNSSS VYSPPSQFP 
      LEPDEDNEN VDNFQEYAG NFEAQPAKE IPPLILPPP 
      NPPVEIYSA VIPIPPGTP VIYTHTPEG PEMMVYTPP 
      VDVQYVSPT PYFYSPTIP TTWYPLGIN SQGFIFPTP 
      VNQNLQNN
    • >TC000504 RB
      MSSSNKKKG KNPAAVDNW LDSIGLYRK NVAYDETCL 
      FRAVSEQLF QCQIYHERV RKECIDYGR RHYYDFWHL 
      LEDENEWYN HLDLLEKHM VVCGNIEIQ LISHKYNRD 
      VVVYDATHQ SVLQVTRRG LDKTLMLCL MNEDHYDVV 
      YEKEHIQNA GFCQSIVYG ILYENVFDV PNVQNIVHG 
      MLYEKDLEK EGDKNTEVV VPPPVNIAP FPFKVAKAL 
      DPTIYRNIE YDSWTEVRR ELRLGDWYY GDDNLILGT 
      RCILNQDDQ TYECYIQEI IKNENKCVV YVTKLAEKR 
      TVNYTDLSP ENDAKPWPL PYRFTKNQV ATPSQAVTL 
      ETKLKLPKK RKDKRRVKS NSESQTQAV SSQQLAQDI 
      EAFIGQPLQ MQNNSGQNY NQAQSPPEI SSSQSQHNL 
      LVTPDIPSP MPKFSWETP WSTAAAASC EKFVWSQPA 
      TSPGMKPMV ASAPTTPNV IPYHDSQYP FVYNYPVDS 
      SFNSWNSSS VYSPPSQFP LEPDEDNEN VDNFQEYAG 
      NFEAQPAKE IPPLILPPP NPPVEIYSA VIPIPPGTP 
      VIYTHTPEG PEMMVYTPP VDVQYVSPT PYFYSPTIP 
      TTWYPLGIN SQGFIFPTP VNQNLQNN
    CDS sequence
    • >TC000504 RA
      atgtctagt agtaataag aaaaagggc aaaaacccg 
      gcagcggtg gacaattgg ttggactca attggcctg 
      tatcgaaaa aatgttgct tatgatgag acttgccta 
      tttcgggct gttagtgaa cagctgttt caatgccaa 
      atctaccac gagagggtc cgtaaagaa tgcatcgat 
      tacggcaga cggcactat tacgatttt tggcactta 
      cttgaggat gaaaatgag tggtacaat catttagac 
      ttgttagaa aagcatatg gtcgtttgt ggtaatata 
      gagattcag ctaattagt cacaagtac aaccgcgac 
      gttgttgtg tacgatgca acacaccaa agcgtactt 
      caagttact cgaagaggt ttagataag acacttatg 
      ctatgcctc atgaacgaa gaccattac gatgtggtt 
      tatgagaag gaacacatc caaaatgcg ggcttttgc 
      caatccatt gtttacggt attctgtac gaaaatgtt 
      tttgatgtt cctaatgtg caaaatatc gtgcatggg 
      atgctgtac gagaaagag ttcgtagct ttatcattt 
      gccgatgtt agtgataat tttgaatct aaacttagc 
      ttggagaaa gagggggac aagaacact gaagttgtt 
      gtgccacca ccagtaaat atagcccct tttcccttc 
      aaggtggct aaagcgctt gatcccacc atctacagg 
      aacattgag tacgattcc tggactgaa gttaggagg 
      gaacttcgt cttggcgac tggtattac ggtgatgac 
      aacctaata ctaggaaca cgctgcatt ctcaatcaa 
      gacgatcaa acttacgaa tgttacatc caagagatc 
      attaaaaac gagaacaaa tgtgtggtt tatgtgaca 
      aaactcgcc gaaaagcgc accgtaaac tacacggat 
      ttgtctcct gaaaacgac gcaaaaccg tggccactg 
      ccctacaga tttaccaaa aaccaagtg gcgactccg 
      tcccaagcc gtcacactc gaaaccaag ctaaaatta 
      ccgaaaaaa cgtaaagat aaacgccga gtgaaaagt 
      aacagcgag tctcaaacg caagcggtt tcatcgcaa 
      cagcttgcc caagatatt gaggctttc attggccag 
      cctttgcaa atgcaaaat aattccgga cagaattac 
      aaccaagcg caaagtccc ccagaaata tccagctcg 
      cagtcgcaa cataatttg ctagtaacc cctgatatt 
      ccatcgccg atgcccaaa ttttcctgg gagactcct 
      tggagcacc gctgctgcg gcctcgtgc gagaaattc 
      gtttggtcg caaccagcg acttccccg gggatgaag 
      ccgatggtg gcctcagct cccaccact ccaaacgtg 
      attccttac cacgattcg caatatccc ttcgtctac 
      aattaccct gtggacagt tcgttcaac tcgtggaac 
      agcagctct gtctacagt cctccgagt caatttccg 
      ctggaaccg gacgaggac aacgaaaat gtggacaat 
      ttccaggag tacgctggg aattttgag gcgcagccg 
      gcgaaagag ataccgcca ctgattctc ccgcctccc 
      aatccgcca gttgagatc tactcggct gtgatcccc 
      attcctccg ggaactccc gtgatttac acacacact 
      cccgaagga ccggaaatg atggtttac acgccgcca 
      gttgatgtg caatacgtt tcgccaaca ccgtacttc 
      tactcgccg acgatacca accacttgg tatccgcta 
      ggaatcaat tcccaggga tttattttc ccaactcca 
      gtcaaccag aatttgcaa aacaattaa
    • >TC000504 RB
      atgtctagt agtaataag aaaaagggc aaaaacccg 
      gcagcggtg gacaattgg ttggactca attggcctg 
      tatcgaaaa aatgttgct tatgatgag acttgccta 
      tttcgggct gttagtgaa cagctgttt caatgccaa 
      atctaccac gagagggtc cgtaaagaa tgcatcgat 
      tacggcaga cggcactat tacgatttt tggcactta 
      cttgaggat gaaaatgag tggtacaat catttagac 
      ttgttagaa aagcatatg gtcgtttgt ggtaatata 
      gagattcag ctaattagt cacaagtac aaccgcgac 
      gttgttgtg tacgatgca acacaccaa agcgtactt 
      caagttact cgaagaggt ttagataag acacttatg 
      ctatgcctc atgaacgaa gaccattac gatgtggtt 
      tatgagaag gaacacatc caaaatgcg ggcttttgc 
      caatccatt gtttacggt attctgtac gaaaatgtt 
      tttgatgtt cctaatgtg caaaatatc gtgcatggg 
      atgctgtac gagaaagac ttggagaaa gagggggac 
      aagaacact gaagttgtt gtgccacca ccagtaaat 
      atagcccct tttcccttc aaggtggct aaagcgctt 
      gatcccacc atctacagg aacattgag tacgattcc 
      tggactgaa gttaggagg gaacttcgt cttggcgac 
      tggtattac ggtgatgac aacctaata ctaggaaca 
      cgctgcatt ctcaatcaa gacgatcaa acttacgaa 
      tgttacatc caagagatc attaaaaac gagaacaaa 
      tgtgtggtt tatgtgaca aaactcgcc gaaaagcgc 
      accgtaaac tacacggat ttgtctcct gaaaacgac 
      gcaaaaccg tggccactg ccctacaga tttaccaaa 
      aaccaagtg gcgactccg tcccaagcc gtcacactc 
      gaaaccaag ctaaaatta ccgaaaaaa cgtaaagat 
      aaacgccga gtgaaaagt aacagcgag tctcaaacg 
      caagcggtt tcatcgcaa cagcttgcc caagatatt 
      gaggctttc attggccag cctttgcaa atgcaaaat 
      aattccgga cagaattac aaccaagcg caaagtccc 
      ccagaaata tccagctcg cagtcgcaa cataatttg 
      ctagtaacc cctgatatt ccatcgccg atgcccaaa 
      ttttcctgg gagactcct tggagcacc gctgctgcg 
      gcctcgtgc gagaaattc gtttggtcg caaccagcg 
      acttccccg gggatgaag ccgatggtg gcctcagct 
      cccaccact ccaaacgtg attccttac cacgattcg 
      caatatccc ttcgtctac aattaccct gtggacagt 
      tcgttcaac tcgtggaac agcagctct gtctacagt 
      cctccgagt caatttccg ctggaaccg gacgaggac 
      aacgaaaat gtggacaat ttccaggag tacgctggg 
      aattttgag gcgcagccg gcgaaagag ataccgcca 
      ctgattctc ccgcctccc aatccgcca gttgagatc 
      tactcggct gtgatcccc attcctccg ggaactccc 
      gtgatttac acacacact cccgaagga ccggaaatg 
      atggtttac acgccgcca gttgatgtg caatacgtt 
      tcgccaaca ccgtacttc tactcgccg acgatacca 
      accacttgg tatccgcta ggaatcaat tcccaggga 
      tttattttc ccaactcca gtcaaccag aatttgcaa 
      aacaattaa
    mRNA sequence
    • >TC000504 RA
      agaataatt gtgcgcatg ctgattact gagtaaaga 
      agtttttta gcaattttc ggagaaacg tggtttgca 
      cgtttacgc taactgaaa aattcagtt agaattcac 
      ttattgtga gtgccaatt ttgccccaa caagtgttt 
      ttgtgtcgg aatgaagta atagtctat atatttttt 
      gcgttcgct atactttga aacaaagaa aatgtctag 
      tagtaataa gaaaaaggg caaaaaccc ggcagcggt 
      ggacaattg gttggactc aattggcct gtatcgaaa 
      aaatgttgc ttatgatga gacttgcct atttcgggc 
      tgttagtga acagctgtt tcaatgcca aatctacca 
      cgagagggt ccgtaaaga atgcatcga ttacggcag 
      acggcacta ttacgattt ttggcactt acttgagga 
      tgaaaatga gtggtacaa tcatttaga cttgttaga 
      aaagcatat ggtcgtttg tggtaatat agagattca 
      gctaattag tcacaagta caaccgcga cgttgttgt 
      gtacgatgc aacacacca aagcgtact tcaagttac 
      tcgaagagg tttagataa gacacttat gctatgcct 
      catgaacga agaccatta cgatgtggt ttatgagaa 
      ggaacacat ccaaaatgc gggcttttg ccaatccat 
      tgtttacgg tattctgta cgaaaatgt ttttgatgt 
      tcctaatgt gcaaaatat cgtgcatgg gatgctgta 
      cgagaaaga gttcgtagc tttatcatt tgccgatgt 
      tagtgataa ttttgaatc taaacttag cttggagaa 
      agaggggga caagaacac tgaagttgt tgtgccacc 
      accagtaaa tatagcccc ttttccctt caaggtggc 
      taaagcgct tgatcccac catctacag gaacattga 
      gtacgattc ctggactga agttaggag ggaacttcg 
      tcttggcga ctggtatta cggtgatga caacctaat 
      actaggaac acgctgcat tctcaatca agacgatca 
      aacttacga atgttacat ccaagagat cattaaaaa 
      cgagaacaa atgtgtggt ttatgtgac aaaactcgc 
      cgaaaagcg caccgtaaa ctacacgga tttgtctcc 
      tgaaaacga cgcaaaacc gtggccact gccctacag 
      atttaccaa aaaccaagt ggcgactcc gtcccaagc 
      cgtcacact cgaaaccaa gctaaaatt accgaaaaa 
      acgtaaaga taaacgccg agtgaaaag taacagcga 
      gtctcaaac gcaagcggt ttcatcgca acagcttgc 
      ccaagatat tgaggcttt cattggcca gcctttgca 
      aatgcaaaa taattccgg acagaatta caaccaagc 
      gcaaagtcc cccagaaat atccagctc gcagtcgca 
      acataattt gctagtaac ccctgatat tccatcgcc 
      gatgcccaa attttcctg ggagactcc ttggagcac 
      cgctgctgc ggcctcgtg cgagaaatt cgtttggtc 
      gcaaccagc gacttcccc ggggatgaa gccgatggt 
      ggcctcagc tcccaccac tccaaacgt gattcctta 
      ccacgattc gcaatatcc cttcgtcta caattaccc 
      tgtggacag ttcgttcaa ctcgtggaa cagcagctc 
      tgtctacag tcctccgag tcaatttcc gctggaacc 
      ggacgagga caacgaaaa tgtggacaa tttccagga 
      gtacgctgg gaattttga ggcgcagcc ggcgaaaga 
      gataccgcc actgattct cccgcctcc caatccgcc 
      agttgagat ctactcggc tgtgatccc cattcctcc 
      gggaactcc cgtgattta cacacacac tcccgaagg 
      accggaaat gatggttta cacgccgcc agttgatgt 
      gcaatacgt ttcgccaac accgtactt ctactcgcc 
      gacgatacc aaccacttg gtatccgct aggaatcaa 
      ttcccaggg atttatttt cccaactcc agtcaacca 
      gaatttgca aaacaatta agagcgttt cattatgaa 
      tgttaatgc tttgaagaa atttatttt tgttat
    • >TC000504 RB
      agaataatt gtgcgcatg ctgattact gagtaaaga 
      agtttttta gcaattttc ggagaaacg tggtttgca 
      cgtttacgc taactgaaa aattcagtt agaattcac 
      ttattgtga gtgccaatt ttgccccaa caagtgttt 
      ttgtgtcgg aatgaagta atagtctat atatttttt 
      gcgttcgct atactttga aacaaagaa aatgtctag 
      tagtaataa gaaaaaggg caaaaaccc ggcagcggt 
      ggacaattg gttggactc aattggcct gtatcgaaa 
      aaatgttgc ttatgatga gacttgcct atttcgggc 
      tgttagtga acagctgtt tcaatgcca aatctacca 
      cgagagggt ccgtaaaga atgcatcga ttacggcag 
      acggcacta ttacgattt ttggcactt acttgagga 
      tgaaaatga gtggtacaa tcatttaga cttgttaga 
      aaagcatat ggtcgtttg tggtaatat agagattca 
      gctaattag tcacaagta caaccgcga cgttgttgt 
      gtacgatgc aacacacca aagcgtact tcaagttac 
      tcgaagagg tttagataa gacacttat gctatgcct 
      catgaacga agaccatta cgatgtggt ttatgagaa 
      ggaacacat ccaaaatgc gggcttttg ccaatccat 
      tgtttacgg tattctgta cgaaaatgt ttttgatgt 
      tcctaatgt gcaaaatat cgtgcatgg gatgctgta 
      cgagaaaga cttggagaa agaggggga caagaacac 
      tgaagttgt tgtgccacc accagtaaa tatagcccc 
      ttttccctt caaggtggc taaagcgct tgatcccac 
      catctacag gaacattga gtacgattc ctggactga 
      agttaggag ggaacttcg tcttggcga ctggtatta 
      cggtgatga caacctaat actaggaac acgctgcat 
      tctcaatca agacgatca aacttacga atgttacat 
      ccaagagat cattaaaaa cgagaacaa atgtgtggt 
      ttatgtgac aaaactcgc cgaaaagcg caccgtaaa 
      ctacacgga tttgtctcc tgaaaacga cgcaaaacc 
      gtggccact gccctacag atttaccaa aaaccaagt 
      ggcgactcc gtcccaagc cgtcacact cgaaaccaa 
      gctaaaatt accgaaaaa acgtaaaga taaacgccg 
      agtgaaaag taacagcga gtctcaaac gcaagcggt 
      ttcatcgca acagcttgc ccaagatat tgaggcttt 
      cattggcca gcctttgca aatgcaaaa taattccgg 
      acagaatta caaccaagc gcaaagtcc cccagaaat 
      atccagctc gcagtcgca acataattt gctagtaac 
      ccctgatat tccatcgcc gatgcccaa attttcctg 
      ggagactcc ttggagcac cgctgctgc ggcctcgtg 
      cgagaaatt cgtttggtc gcaaccagc gacttcccc 
      ggggatgaa gccgatggt ggcctcagc tcccaccac 
      tccaaacgt gattcctta ccacgattc gcaatatcc 
      cttcgtcta caattaccc tgtggacag ttcgttcaa 
      ctcgtggaa cagcagctc tgtctacag tcctccgag 
      tcaatttcc gctggaacc ggacgagga caacgaaaa 
      tgtggacaa tttccagga gtacgctgg gaattttga 
      ggcgcagcc ggcgaaaga gataccgcc actgattct 
      cccgcctcc caatccgcc agttgagat ctactcggc 
      tgtgatccc cattcctcc gggaactcc cgtgattta 
      cacacacac tcccgaagg accggaaat gatggttta 
      cacgccgcc agttgatgt gcaatacgt ttcgccaac 
      accgtactt ctactcgcc gacgatacc aaccacttg 
      gtatccgct aggaatcaa ttcccaggg atttatttt 
      cccaactcc agtcaacca gaatttgca aaacaatta 
      agagcgttt cattatgaa tgttaatgc tttgaagaa 
      atttatttt tgttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY