Status
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Gene information
General information
TC000495 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10586329..10588458
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000495
    iBeetle screen iB_00108

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    embryo/egg condensed embryonic tissue, no cuticle, cns fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    remarks: later than schedule, hours 100
    Adult morphology
    remarks: later than schedule, hours 100
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000495 RA
      MRCYRIQLF VVCSLLFFI NINAQRRSS CTTPNGDIA 
      RCIPISSCP ILYDAVTTR DKQQLKFLK ESQCGYGRD 
      PLVCCGLHN NYRLNPGDF AYDDEPFGW GNSHHRRNS 
      GSQNWDRDD TPSWGNERK NNNNNNEWS TNNRRRTTT 
      QRTNSEESG WGISDRPNR RTSGEESRW STSDRPNRR 
      TSGEESGWG TTDRPNRRT SGEDSSRRN NNRRTTTQR 
      TNSEESSFE REDDRFNDR TNSGSNSNR QTSQGSGST 
      DKSECGVQE VDRILDGQA TDLREFPWM ALLQYRKKS 
      GNLVFSCGG TLISPRYVL TAAHCVRGQ ILTKIGPLV 
      NVRLGEYNT ETERDCSNQ MGFEICNEK PIDSEIDKV 
      IPHPDYSDN SADRYHDIA LIKLKRQVS YTDFIKPIC 
      LPGKSEKTS VGKRLAVAG WGRTEYASN SPVKLKLWV 
      PVAETSQCS SKFKSAGVT LGNRQLCAG GEQGRDSCN 
      GDSGGPLMA VRNATAQWY IEGIVSFGA RCGSEGWPG 
      IYTRVSEYL DWIQNNTN
    • >TC000495 RB
      MRCYRIQLF VVCSLLFFI NINAQFFLE RRSSCTTPN 
      GDIARCIPI SSCPILYDA VTTRDKQQL KFLKESQCG 
      YGRDPLVCC GLHNNYRLN PGDFAYDDE PFGWGNSHH 
      RRNSGSQNW DRDDTPSWG NERKNNNNN NEWSTNNRR 
      RTTTQRTNS EESGWGISD RPNRRTSGE ESRWSTSDR 
      PNRRTSGEE SGWGTTDRP NRRTSGEDS SRRNNNRRT 
      TTQRTNSEE SSFEREDDR FNDRTNSGS NSNRQTSQG 
      SGSTDKSEC GVQEVDRIL DGQATDLRE FPWMALLQY 
      RKKSGNLVF SCGGTLISP RYVLTAAHC VRGQILTKI 
      GPLVNVRLG EYNTETERD CSNQMGFEI CNEKPIDSE 
      IDKVIPHPD YSDNSADRY HDIALIKLK RQVSYTDFI 
      KPICLPGKS EKTSVGKRL AVAGWGRTE YASNSPVKL 
      KLWVPVAET SQCSSKFKS AGVTLGNRQ LCAGGEQGR 
      DSCNGDSGG PLMAVRNAT AQWYIEGIV SFGARCGSE 
      GWPGIYTRV SEYLDWIQN NTN
    CDS sequence
    • >TC000495 RA
      atgcggtgt tacagaata cagttgttt gtggtttgt 
      tcacttctt ttcttcatt aatattaac gcacaacga 
      agatcgtca tgtacgact cctaacggc gacatagca 
      cggtgtatc ccaattagc tcctgtcct attctttac 
      gatgctgtc acaactcga gataagcaa cagttaaag 
      tttttgaaa gaatcacag tgtggatat ggccgggat 
      cctttggtt tgttgcggt cttcataac aattatcgt 
      ctaaaccct ggcgatttt gcctacgat gatgaaccc 
      ttcggctgg ggtaactcc caccataga aggaactca 
      gggtcgcaa aactgggac agggacgat actccaagc 
      tggggcaat gaaagaaaa aataataat aataataac 
      gaatggagc actaataac aggagaaga actacaact 
      caaagaaca aattcggaa gaatctgga tggggtatt 
      agtgacaga ccaaacaga agaacaagc ggtgaagaa 
      tctagatgg agtactagt gatagacca aacagaaga 
      acaagcggt gaagaatct ggatggggt actactgac 
      agaccaaat agacgaaca agcggtgaa gattcgagc 
      cgtcgtaac aataacaga agaacgaca acacaacga 
      acaaattcc gaggaatct agttttgaa agagaggat 
      gacagattc aatgatcga actaattcg ggatcaaac 
      agtaataga caaacaagt caaggttct ggtagtacc 
      gataagtcc gaatgtgga gtccaggaa gttgatcga 
      attctcgac gggcaggcc accgatttg cgagaattc 
      ccttggatg gctttgtta cagtaccgc aaaaagtcc 
      ggaaatttg gtattttcc tgtggcgga actctcatt 
      tcacctcgc tacgtttta acagcagct cactgcgtc 
      cgaggacaa atccttaca aaaatcggg ccattggtc 
      aatgtcagg ctaggcgag tacaacact gaaacagag 
      cgagactgt agtaaccaa atgggattc gaaatttgt 
      aacgagaaa ccaatagat tcagagatt gataaagtg 
      ataccccat cccgactac agcgataac agtgcggac 
      agataccat gacattgct ttaatcaaa ttgaagcga 
      caagtctcg tacactgat tttattaag cccatttgt 
      ttgcccgga aaatcggaa aaaacgagt gttggtaaa 
      cggctagcg gtagctgga tggggccga acggagtac 
      gccagtaat agtccagtc aaactaaaa ctgtgggtt 
      ccagtggcg gaaacctcc cagtgttcc agcaaattt 
      aaatcggct ggagtgact ttagggaat agacagttg 
      tgtgctgga ggagagcaa ggcagagat tcttgtaat 
      ggggacagt ggtgggcct ttaatggcg gtcagaaat 
      gcgacagcc caatggtac attgaagga attgtctcg 
      tttggagcg agatgtggt agcgaagga tggccagga 
      atttacaca cgggtgtcg gagtatctc gactggatt 
      caaaacaat accaattaa
    • >TC000495 RB
      atgcggtgt tacagaata cagttgttt gtggtttgt 
      tcacttctt ttcttcatt aatattaac gcacaattt 
      tttttagaa cgaagatcg tcatgtacg actcctaac 
      ggcgacata gcacggtgt atcccaatt agctcctgt 
      cctattctt tacgatgct gtcacaact cgagataag 
      caacagtta aagtttttg aaagaatca cagtgtgga 
      tatggccgg gatcctttg gtttgttgc ggtcttcat 
      aacaattat cgtctaaac cctggcgat tttgcctac 
      gatgatgaa cccttcggc tggggtaac tcccaccat 
      agaaggaac tcagggtcg caaaactgg gacagggac 
      gatactcca agctggggc aatgaaaga aaaaataat 
      aataataat aacgaatgg agcactaat aacaggaga 
      agaactaca actcaaaga acaaattcg gaagaatct 
      ggatggggt attagtgac agaccaaac agaagaaca 
      agcggtgaa gaatctaga tggagtact agtgataga 
      ccaaacaga agaacaagc ggtgaagaa tctggatgg 
      ggtactact gacagacca aatagacga acaagcggt 
      gaagattcg agccgtcgt aacaataac agaagaacg 
      acaacacaa cgaacaaat tccgaggaa tctagtttt 
      gaaagagag gatgacaga ttcaatgat cgaactaat 
      tcgggatca aacagtaat agacaaaca agtcaaggt 
      tctggtagt accgataag tccgaatgt ggagtccag 
      gaagttgat cgaattctc gacgggcag gccaccgat 
      ttgcgagaa ttcccttgg atggctttg ttacagtac 
      cgcaaaaag tccggaaat ttggtattt tcctgtggc 
      ggaactctc atttcacct cgctacgtt ttaacagca 
      gctcactgc gtccgagga caaatcctt acaaaaatc 
      gggccattg gtcaatgtc aggctaggc gagtacaac 
      actgaaaca gagcgagac tgtagtaac caaatggga 
      ttcgaaatt tgtaacgag aaaccaata gattcagag 
      attgataaa gtgataccc catcccgac tacagcgat 
      aacagtgcg gacagatac catgacatt gctttaatc 
      aaattgaag cgacaagtc tcgtacact gattttatt 
      aagcccatt tgtttgccc ggaaaatcg gaaaaaacg 
      agtgttggt aaacggcta gcggtagct ggatggggc 
      cgaacggag tacgccagt aatagtcca gtcaaacta 
      aaactgtgg gttccagtg gcggaaacc tcccagtgt 
      tccagcaaa tttaaatcg gctggagtg actttaggg 
      aatagacag ttgtgtgct ggaggagag caaggcaga 
      gattcttgt aatggggac agtggtggg cctttaatg 
      gcggtcaga aatgcgaca gcccaatgg tacattgaa 
      ggaattgtc tcgtttgga gcgagatgt ggtagcgaa 
      ggatggcca ggaatttac acacgggtg tcggagtat 
      ctcgactgg attcaaaac aataccaat taa
    mRNA sequence
    • >TC000495 RA
      taaactcga aagaccagt ggcttgctt cttagttca 
      acaaacatc gtcaaggcg gacggcagc gataaaatt 
      atgcggtgt tacagaata cagttgttt gtggtttgt 
      tcacttctt ttcttcatt aatattaac gcacaacga 
      agatcgtca tgtacgact cctaacggc gacatagca 
      cggtgtatc ccaattagc tcctgtcct attctttac 
      gatgctgtc acaactcga gataagcaa cagttaaag 
      tttttgaaa gaatcacag tgtggatat ggccgggat 
      cctttggtt tgttgcggt cttcataac aattatcgt 
      ctaaaccct ggcgatttt gcctacgat gatgaaccc 
      ttcggctgg ggtaactcc caccataga aggaactca 
      gggtcgcaa aactgggac agggacgat actccaagc 
      tggggcaat gaaagaaaa aataataat aataataac 
      gaatggagc actaataac aggagaaga actacaact 
      caaagaaca aattcggaa gaatctgga tggggtatt 
      agtgacaga ccaaacaga agaacaagc ggtgaagaa 
      tctagatgg agtactagt gatagacca aacagaaga 
      acaagcggt gaagaatct ggatggggt actactgac 
      agaccaaat agacgaaca agcggtgaa gattcgagc 
      cgtcgtaac aataacaga agaacgaca acacaacga 
      acaaattcc gaggaatct agttttgaa agagaggat 
      gacagattc aatgatcga actaattcg ggatcaaac 
      agtaataga caaacaagt caaggttct ggtagtacc 
      gataagtcc gaatgtgga gtccaggaa gttgatcga 
      attctcgac gggcaggcc accgatttg cgagaattc 
      ccttggatg gctttgtta cagtaccgc aaaaagtcc 
      ggaaatttg gtattttcc tgtggcgga actctcatt 
      tcacctcgc tacgtttta acagcagct cactgcgtc 
      cgaggacaa atccttaca aaaatcggg ccattggtc 
      aatgtcagg ctaggcgag tacaacact gaaacagag 
      cgagactgt agtaaccaa atgggattc gaaatttgt 
      aacgagaaa ccaatagat tcagagatt gataaagtg 
      ataccccat cccgactac agcgataac agtgcggac 
      agataccat gacattgct ttaatcaaa ttgaagcga 
      caagtctcg tacactgat tttattaag cccatttgt 
      ttgcccgga aaatcggaa aaaacgagt gttggtaaa 
      cggctagcg gtagctgga tggggccga acggagtac 
      gccagtaat agtccagtc aaactaaaa ctgtgggtt 
      ccagtggcg gaaacctcc cagtgttcc agcaaattt 
      aaatcggct ggagtgact ttagggaat agacagttg 
      tgtgctgga ggagagcaa ggcagagat tcttgtaat 
      ggggacagt ggtgggcct ttaatggcg gtcagaaat 
      gcgacagcc caatggtac attgaagga attgtctcg 
      tttggagcg agatgtggt agcgaagga tggccagga 
      atttacaca cgggtgtcg gagtatctc gactggatt 
      caaaacaat accaattaa actgactct catagttgt 
      tgtgtatat ttatttatg atagcgctt aattaagtt 
      cggaataaa acattaaat taacggacc agaaatttt 
      ctcaagtga ggtacaagt gtattttat attaaagtt 
      tatcaactg gtgataacc ttataaaaa tacttaaca 
      gcatcgtag ttaatgcca tcgtcaata agcttgtta 
      tgaaaacaa gagacgtat ttaattaaa aaaataata 
      aaattttag tgaaatt
    • >TC000495 RB
      taaactcga aagaccagt ggcttgctt cttagttca 
      acaaacatc gtcaaggcg gacggcagc gataaaatt 
      atgcggtgt tacagaata cagttgttt gtggtttgt 
      tcacttctt ttcttcatt aatattaac gcacaattt 
      tttttagaa cgaagatcg tcatgtacg actcctaac 
      ggcgacata gcacggtgt atcccaatt agctcctgt 
      cctattctt tacgatgct gtcacaact cgagataag 
      caacagtta aagtttttg aaagaatca cagtgtgga 
      tatggccgg gatcctttg gtttgttgc ggtcttcat 
      aacaattat cgtctaaac cctggcgat tttgcctac 
      gatgatgaa cccttcggc tggggtaac tcccaccat 
      agaaggaac tcagggtcg caaaactgg gacagggac 
      gatactcca agctggggc aatgaaaga aaaaataat 
      aataataat aacgaatgg agcactaat aacaggaga 
      agaactaca actcaaaga acaaattcg gaagaatct 
      ggatggggt attagtgac agaccaaac agaagaaca 
      agcggtgaa gaatctaga tggagtact agtgataga 
      ccaaacaga agaacaagc ggtgaagaa tctggatgg 
      ggtactact gacagacca aatagacga acaagcggt 
      gaagattcg agccgtcgt aacaataac agaagaacg 
      acaacacaa cgaacaaat tccgaggaa tctagtttt 
      gaaagagag gatgacaga ttcaatgat cgaactaat 
      tcgggatca aacagtaat agacaaaca agtcaaggt 
      tctggtagt accgataag tccgaatgt ggagtccag 
      gaagttgat cgaattctc gacgggcag gccaccgat 
      ttgcgagaa ttcccttgg atggctttg ttacagtac 
      cgcaaaaag tccggaaat ttggtattt tcctgtggc 
      ggaactctc atttcacct cgctacgtt ttaacagca 
      gctcactgc gtccgagga caaatcctt acaaaaatc 
      gggccattg gtcaatgtc aggctaggc gagtacaac 
      actgaaaca gagcgagac tgtagtaac caaatggga 
      ttcgaaatt tgtaacgag aaaccaata gattcagag 
      attgataaa gtgataccc catcccgac tacagcgat 
      aacagtgcg gacagatac catgacatt gctttaatc 
      aaattgaag cgacaagtc tcgtacact gattttatt 
      aagcccatt tgtttgccc ggaaaatcg gaaaaaacg 
      agtgttggt aaacggcta gcggtagct ggatggggc 
      cgaacggag tacgccagt aatagtcca gtcaaacta 
      aaactgtgg gttccagtg gcggaaacc tcccagtgt 
      tccagcaaa tttaaatcg gctggagtg actttaggg 
      aatagacag ttgtgtgct ggaggagag caaggcaga 
      gattcttgt aatggggac agtggtggg cctttaatg 
      gcggtcaga aatgcgaca gcccaatgg tacattgaa 
      ggaattgtc tcgtttgga gcgagatgt ggtagcgaa 
      ggatggcca ggaatttac acacgggtg tcggagtat 
      ctcgactgg attcaaaac aataccaat taaactgac 
      tctcatagt tgttgtgta tatttattt atgatagcg 
      cttaattaa gttcggaat aaaacatta aattaacgg 
      accagaaat tttctcaag tgaggtaca agtgtattt 
      tatattaaa gtttatcaa ctggtgata accttataa 
      aaatactta acagcatcg tagttaatg ccatcgtca 
      ataagcttg ttatgaaaa caagagacg tatttaatt 
      aaaaaaata ataaaattt tagtgaaat t
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