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Gene information
General information
TC000481 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 5274191..5278040
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000481
    iBeetle screen iB_00106
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000481 RA
      MSKRRIEVL DPFIKKKRE ERGISTAVT NTPQVTNAS 
      INPHTGLPY TNKYHELYR KRITLPVFE YRNDFMRLL 
      AENQCIVLV GETGSGKTT QIPQWCVEF ARSVGKKGV 
      CCTQPRRVA AMSVAQRVS EEMDVALGQ EVGYSIRFE 
      DCSSAKTIL KYMTDGMLL REGMSDPML DAYQCILLD 
      EAHERTLAT DILMGVLKE VIKQRSDLK LVIMSATLD 
      AGKFQQYFD NAPLMNVPG RTHPVEIFY TPEPERDYL 
      EAAIRTVIQ IHMCEEIAG DILLFLTGQ EEIEVACKR 
      IKREIDNLG PEVGELKCI PLYSTLPPN LQQRIFEEA 
      PPNKANGAI GRKVVVSTN IAETSLTID GVVFVIDPG 
      FAKQKVYNP RIRVESLLV SPISKASAQ QRAGRAGRT 
      RPGKCFRLY TEKAYKNEM QDNTYPEIL RSNLGSVVL 
      QLKKLGIDD LVHFDFMDP PAPETLMRA LELLNYLAA 
      LDDDGNLTD LGAVMAEFP LDPQLAKML IASCNHNCS 
      NEILSITAM LSVPQCFIR PNEAKKAAD DAKMRFAHI 
      DGDHLTLLN VYHAFKQSM EDPQWCYDN FVNYRSLKS 
      ADNVRQQLS RIMDRFNLK RTSTDFTSK DYYINIRKA 
      LVNGFFMQV AHLERTGHY LTIKDNQNV QLHPSTCLD 
      HKPEWVIYN EFVLTTKNY IRTVTDIKP DWLIKIAPQ 
      YYDLQNFPQ CEAKRQLEI IQNRLDSKQ YQHGF
    CDS sequence
    • >TC000481 RA
      atgtctaaa aggagaatc gaagtgttg gatccgttt 
      ataaagaag aaaagggaa gaacgtggg attagcact 
      gcagtaacg aacaccccg caagtaacc aacgcctca 
      atcaacccg cacaccggt ttgccgtac accaataag 
      taccatgag ttgtatcgc aaacgcatc acgcttcct 
      gtctttgaa taccgcaac gatttcatg cgtttgctc 
      gccgaaaac cagtgcatt gtgctggtt ggcgagaca 
      gggtcgggg aaaacgacg caaattcca cagtggtgc 
      gttgaattc gccaggtcg gtcgggaaa aagggggtg 
      tgctgcacc cagcccagg agggtggct gccatgtct 
      gtggcccag agagtgtca gaggaaatg gacgtcgcc 
      ttggggcaa gaggtgggc tacagcatt cggttcgag 
      gactgctcg tcggccaaa actatcctc aagtacatg 
      accgacggt atgttgctc cgtgagggc atgagcgac 
      ccaatgctg gacgcctac cagtgcatt ttgctcgac 
      gaagcccac gagcgcacc cttgccacc gacattctg 
      atgggtgtt ttaaaagaa gttattaaa caacgctcc 
      gacttgaaa ttagttatt atgtcggcg actctagac 
      gctggcaaa ttccagcaa tatttcgat aacgctccc 
      ttaatgaac gtccccggt cgtacgcat ccagtagaa 
      attttctat actcccgaa ccggaacgc gattatctc 
      gaagccgcc atccgtacc gtcatccaa atccacatg 
      tgtgaggaa atagccggc gatattctc ctatttttg 
      accggtcag gaggaaatc gaagttgct tgcaaacga 
      atcaaacgc gaaattgat aatctgggc cccgaagtg 
      ggcgaacta aaatgtatc ccgttgtat tcgacgctg 
      cctccgaat ttgcagcaa cgtattttc gaggaggcg 
      ccccctaat aaggccaat ggggcgatc ggacgcaaa 
      gttgtcgtc tcgaccaac attgcggag acttccttg 
      actatagac ggtgtcgtg tttgtgata gatcccgga 
      tttgcgaaa caaaaagtc tacaatcct cgaattcgt 
      gtcgagtcg ttattggtc tcgccgatt agtaaagcc 
      tcagcacag cagcgtgcc ggaagagct ggaaggact 
      cggccgggg aaatgtttc cgactttac accgaaaaa 
      gcatacaag aacgaaatg caagacaac acctatcca 
      gaaatttta cgatcgaat ttgggttcg gtcgtttta 
      caattgaag aaactggga attgatgat ttggttcat 
      tttgacttc atggatcca ccggcgcct gaaaccttg 
      atgagggcc ttggaattg ttgaattat ttggcggcg 
      ttggatgat gatggaaat ctcaccgat ttgggggcg 
      gttatggcc gagtttccg ctcgatcca caattggcg 
      aaaatgctc attgccagc tgcaaccac aactgttcc 
      aatgagata ctgtccatt acggcgatg ttgtcagtc 
      ccacagtgc ttcatccga ccaaatgag gcgaagaaa 
      gcggccgac gatgcaaaa atgagattc gcgcatatt 
      gacggggac catttgacg ctgctcaat gtctaccat 
      gctttcaaa caaagtatg gaagatcca caatggtgc 
      tatgataac tttgtcaat taccgttcc ttgaaatct 
      gccgataac gtgagacaa cagttgtca agaattatg 
      gaccggttt aatctcaag cgaacttcg acagatttc 
      accagcaag gattattat attaatata aggaaggct 
      ttagttaac gggtttttc atgcaggtg gcgcatttg 
      gaaagaacc ggtcactac cttactatc aaggacaac 
      caaaatgtt caactacac ccgtccaca tgtttggat 
      cacaaaccg gagtgggtt atttacaat gagttcgtt 
      ttgaccacg aaaaattac atcaggacc gtgacagat 
      attaaaccg gactggctc attaaaata gccccccag 
      tattacgat cttcaaaat ttcccacaa tgtgaagct 
      aaacgtcaa ctggaaata atacaaaat cgtctagat 
      tctaaacaa taccaacac ggtttttag
    mRNA sequence
    • >TC000481 RA
      caaagttgc atggagtcg tggtcgtag ttttatttc 
      caaacaaac tttaatgca atttatttt tatttgtta 
      acaagtaga caattcgag agcttgtta ctattagcc 
      gtagtctat ctaacaatg tctaaaagg agaatcgaa 
      gtgttggat ccgtttata aagaagaaa agggaagaa 
      cgtgggatt agcactgca gtaacgaac accccgcaa 
      gtaaccaac gcctcaatc aacccgcac accggtttg 
      ccgtacacc aataagtac catgagttg tatcgcaaa 
      cgcatcacg cttcctgtc tttgaatac cgcaacgat 
      ttcatgcgt ttgctcgcc gaaaaccag tgcattgtg 
      ctggttggc gagacaggg tcggggaaa acgacgcaa 
      attccacag tggtgcgtt gaattcgcc aggtcggtc 
      gggaaaaag ggggtgtgc tgcacccag cccaggagg 
      gtggctgcc atgtctgtg gcccagaga gtgtcagag 
      gaaatggac gtcgccttg gggcaagag gtgggctac 
      agcattcgg ttcgaggac tgctcgtcg gccaaaact 
      atcctcaag tacatgacc gacggtatg ttgctccgt 
      gagggcatg agcgaccca atgctggac gcctaccag 
      tgcattttg ctcgacgaa gcccacgag cgcaccctt 
      gccaccgac attctgatg ggtgtttta aaagaagtt 
      attaaacaa cgctccgac ttgaaatta gttattatg 
      tcggcgact ctagacgct ggcaaattc cagcaatat 
      ttcgataac gctccctta atgaacgtc cccggtcgt 
      acgcatcca gtagaaatt ttctatact cccgaaccg 
      gaacgcgat tatctcgaa gccgccatc cgtaccgtc 
      atccaaatc cacatgtgt gaggaaata gccggcgat 
      attctccta tttttgacc ggtcaggag gaaatcgaa 
      gttgcttgc aaacgaatc aaacgcgaa attgataat 
      ctgggcccc gaagtgggc gaactaaaa tgtatcccg 
      ttgtattcg acgctgcct ccgaatttg cagcaacgt 
      attttcgag gaggcgccc cctaataag gccaatggg 
      gcgatcgga cgcaaagtt gtcgtctcg accaacatt 
      gcggagact tccttgact atagacggt gtcgtgttt 
      gtgatagat cccggattt gcgaaacaa aaagtctac 
      aatcctcga attcgtgtc gagtcgtta ttggtctcg 
      ccgattagt aaagcctca gcacagcag cgtgccgga 
      agagctgga aggactcgg ccggggaaa tgtttccga 
      ctttacacc gaaaaagca tacaagaac gaaatgcaa 
      gacaacacc tatccagaa attttacga tcgaatttg 
      ggttcggtc gttttacaa ttgaagaaa ctgggaatt 
      gatgatttg gttcatttt gacttcatg gatccaccg 
      gcgcctgaa accttgatg agggccttg gaattgttg 
      aattatttg gcggcgttg gatgatgat ggaaatctc 
      accgatttg ggggcggtt atggccgag tttccgctc 
      gatccacaa ttggcgaaa atgctcatt gccagctgc 
      aaccacaac tgttccaat gagatactg tccattacg 
      gcgatgttg tcagtccca cagtgcttc atccgacca 
      aatgaggcg aagaaagcg gccgacgat gcaaaaatg 
      agattcgcg catattgac ggggaccat ttgacgctg 
      ctcaatgtc taccatgct ttcaaacaa agtatggaa 
      gatccacaa tggtgctat gataacttt gtcaattac 
      cgttccttg aaatctgcc gataacgtg agacaacag 
      ttgtcaaga attatggac cggtttaat ctcaagcga 
      acttcgaca gatttcacc agcaaggat tattatatt 
      aatataagg aaggcttta gttaacggg tttttcatg 
      caggtggcg catttggaa agaaccggt cactacctt 
      actatcaag gacaaccaa aatgttcaa ctacacccg 
      tccacatgt ttggatcac aaaccggag tgggttatt 
      tacaatgag ttcgttttg accacgaaa aattacatc 
      aggaccgtg acagatatt aaaccggac tggctcatt 
      aaaatagcc ccccagtat tacgatctt caaaatttc 
      ccacaatgt gaagctaaa cgtcaactg gaaataata 
      caaaatcgt ctagattct aaacaatac caacacggt 
      ttttagttt tgtttaaaa atgcctatg ttattgcaa 
      tttggtgtg tatttgcgt tttacttag tcttcattc 
      cacgaaaat aaactgatg ttcaataat aatttattc 
      actattata tgtatattt ggataaatc tataaactg 
      gcttgtgtt aatattcta gcgttgatg ttacatttt 
      gctacagca ttctacgtt tcgaaaata tatgtataa 
      atgtttagc gataaaatt gaaggtttg agctcagaa 
      attgtactt tataagtat gttattggc accaataaa 
      gagtcgata ttttt