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Gene information
General information
TC000462 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 5491866..5493187
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000462
    iBeetle screen iB_00098
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000462 RA
      MATNKLSML VRSFSTSNV ASQMVKPPV 
      QVFGIDGRY ATALYSAAS KQKTLDSVE 
      KDLLKFQSA IKSDPKLRE FIKNPTIKR 
      GLKSEALKQ IASKISLNA QTSNLLQTL 
      ADNGRLKNL DGVINAFKV MMAAHRGEV 
      TCEVITARD LDAAQKSKL ESVLKSFVK 
      SSETIQLST KVDPSIIGG MIVSIGDKY 
      VDMSVASKI KKYTEVIST PV
    CDS sequence
    • >TC000462 RA
      atggccaca aataagctc tcgatgctt gtccgctcc 
      ttcagcact agcaatgtc gcaagtcag atggttaag 
      cctccggtg caggtcttt ggcattgac ggacgttac 
      gccaccgcc ctgtactcg gctgccagc aagcaaaaa 
      accctcgat tctgttgaa aaagacctc ctaaagttc 
      cagtcagct atcaagtct gaccccaaa ttgagggaa 
      tttatcaag aaccccaca attaagcgc gggctgaaa 
      tccgaggct ttgaaacaa attgcctcg aaaatatcg 
      cttaacgcg cagacgtcc aatcttttg cagactctt 
      gccgataat gggcgtctg aagaatttg gatggggtt 
      atcaacgcc tttaaggtc atgatggca gcccatcgt 
      ggagaggtg acttgtgag gtcatcaca gccagagac 
      ttagatgct gcgcaaaag tctaagttg gaatcggtc 
      ttaaaatcg ttcgtgaag tcaagcgag accattcag 
      ttgagtacc aaagttgat ccctctatc ataggagga 
      atgatcgtc agcattggt gataaatac gttgatatg 
      agcgtcgct agcaaaatc aagaagtac acagaagtc 
      atttccact cctgtttaa
    mRNA sequence
    • >TC000462 RA
      tcagcgttg ctactgata tgttttaca tttgtttaa 
      cgtgtgaat tgttttaaa ttggttaaa actaatctt 
      aatgattta ttttaagta gtttcagct taaccttag 
      catatttgt gcttagtcg ctgggcttg ctgacaata 
      agaggggag cggtaccgc caattactt aaattgcga 
      gagtcgagg ggaaatttt aaaacatcg gggtagtca 
      cgctattaa ttatcgatt ccaaacgaa atctataac 
      cacttcatt ttattttta attaatttt acggaaaaa 
      ttgaaaaaa tgtcactgt caaactgac atttccaaa 
      acgtttcac caacatcga caagtcaca ccatcaaag 
      accctagtt tttgacagt cgaagggtt gaacttgac 
      agtgcagtg gggccctaa gttcgattt cacttccag 
      caaccaaaa aaacgcaat aatggccac aaataagct 
      ctcgatgct tgtccgctc cttcagcac tagcaatgt 
      cgcaagtca gatggttaa gcctccggt gcaggtctt 
      tggcattga cggacgtta cgccaccgc cctgtactc 
      ggctgccag caagcaaaa aaccctcga ttctgttga 
      aaaagacct cctaaagtt ccagtcagc tatcaagtc 
      tgaccccaa attgaggga atttatcaa gaaccccac 
      aattaagcg cgggctgaa atccgaggc tttgaaaca 
      aattgcctc gaaaatatc gcttaacgc gcagacgtc 
      caatctttt gcagactct tgccgataa tgggcgtct 
      gaagaattt ggatggggt tatcaacgc ctttaaggt 
      catgatggc agcccatcg tggagaggt gacttgtga 
      ggtcatcac agccagaga cttagatgc tgcgcaaaa 
      gtctaagtt ggaatcggt cttaaaatc gttcgtgaa 
      gtcaagcga gaccattca gttgagtac caaagttga 
      tccctctat cataggagg aatgatcgt cagcattgg 
      tgataaata cgttgatat gagcgtcgc tagcaaaat 
      caagaagta cacagaagt catttccac tcctgttta 
      aattaatca ttacggtta tagaagttg catagttcg 
      ccaataaaa cgtcataat ttca