Status
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Gene information
General information
TC000459 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11037164..11040630
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000459
    iBeetle screen iB_00097

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000459 RA
      MSPPNGAPP GTAETEYLK VPTEPKPTP EEQQPLNDL 
      VPEDDAEVV VPPDGGWGW VVVFASFMC NMIVDGIIF 
      SFGAFIEPI KDEFHVTKA YVALVGSLM SGFYLIAGP 
      FASAVANRY GFRLVAIMG SVLGAAAFA LANFAPNVE 
      YLCVMFGVI GGIGFGFIY VPSIITVGF YFEKWRALA 
      TGIGVCGSG IGTFMFAPI TAYLIENLG WRTAILCQG 
      TIVFACGSF GFLYRPLKP TKVHDIKGE GETELKMDP 
      SKLPPITKM KMELALEAM KSNESCHNS QASINRMLG 
      VNNNANYPR VSDVYHTIS IPQQRALDG SSYEKRLSV 
      PFISEADRM KNTGAKECL LQKSANPII VPKGSRRGS 
      AVDPEVNRP MYRDDIFFN ASLTRLPQY TSRTSIAYN 
      LSVTRLPTK NDIEEEKKS SCKMCPEAV KRTLATMLD 
      YSLLCSPTF LLLALGGFF TMMGFYVPF MFLVSRAEN 
      ANLAKEYTR FLISAIGIS NTIGRVLCG VLSSFPGVN 
      ALFVTNAAL TIGGIATIF SGFSMTAEY QFFYTAVFG 
      LAISTFASL RSIVVVDLL GLEKLTNAF GLLLLFQGV 
      AAIFGAPIA GAFTEATGS YDAGFYLSG SLILASAVM 
      CYPLNYINR REMQKKKEM VENKSVPV
    CDS sequence
    • >TC000459 RA
      atgtcgccc ccgaacggt gctcctcca ggaaccgcc 
      gaaaccgag tacctcaaa gtgcccacc gaacccaaa 
      ccgaccccc gaagagcaa caacccttg aacgacctc 
      gtccccgag gacgatgca gaagtggtc gtgcccccc 
      gacgggggc tggggctgg gtcgtggtt ttcgcctct 
      ttcatgtgc aatatgatc gtcgatggg atcattttc 
      tcgtttggg gctttcatc gagcccatc aaagacgaa 
      tttcacgtc acgaaagca tatgtggcg cttgtgggg 
      tctctcatg tccgggttt tatcttatc gctgggccc 
      tttgccagc gctgtcgcc aacagatat gggtttaga 
      ctggtggcc atcatgggg agcgtcttg ggggcggcg 
      gccttcgcc ttggccaac ttcgccccc aacgtggag 
      tatctctgc gtcatgttt ggcgttata ggagggatt 
      ggtttcggg tttatctac gtgccgtcg atcatcacc 
      gttgggttt tatttcgag aaatggcgg gccttggcg 
      accggaatc ggggtttgc ggttccggt atcggaacc 
      tttatgttt gcccccata acagcatac ctgatcgag 
      aaccttggt tggagaact gcgattttg tgccaaggc 
      actattgtg ttcgcttgt ggcagtttt gggttcctc 
      tacaggccg ttaaaaccg acaaaagtc catgatatt 
      aagggtgag ggagagact gaacttaaa atggatccg 
      agcaaactg ccccccatt actaagatg aagatggag 
      ctggctctc gaagccatg aaaagtaat gagtcttgt 
      cacaacagc caagcctca atcaaccgc atgctgggg 
      gttaacaac aacgcgaac tacccccga gtctcggac 
      gtgtaccat acgattagc attccgcag caaagggcc 
      ctggacggg tccagttac gagaaacgg ttgagcgtg 
      ccgtttatt tccgaagcc gatcggatg aaaaacaca 
      ggagcgaag gaatgcttg ctccagaag agtgcgaac 
      ccgattata gttcctaaa ggttcaaga cggggttct 
      gcggttgac cccgaggtt aatcgacca atgtaccga 
      gacgatatc ttcttcaac gcttcctta acacgttta 
      cctcaatac acctctcgg acatcaatc gcctacaac 
      ttatcagtc acgcggtta ccgacgaaa aacgacatc 
      gaggaagag aagaaaagt tcttgcaaa atgtgccca 
      gaagccgtc aaacgaact ctcgccaca atgctggac 
      tacagtcta ctttgctcc ccaaccttc ctcttgcta 
      gcccttggc ggcttcttc accatgatg ggtttctac 
      gtcccgttc atgttcctc gtaagccgg gccgagaac 
      gcgaacctg gcaaaagag tacacgagg tttctcatc 
      tcagctatt ggcatttcc aacacaatc gggcgtgtt 
      ttgtgcggc gtccttagc tctttcccc ggtgttaac 
      gctctgttt gtgacaaac gctgcgctg acgatcggc 
      ggaatcgca acaatattc agcggtttt tccatgact 
      gctgaatac caattcttc tacaccgcg gtgtttggt 
      ttagctatt tcaaccttt gcgtcgctg aggtcaatc 
      gttgtggtg gatcttctg gggctggag aagttaacg 
      aatgcgttt gggttgctt ttgctgttt caaggagtg 
      gcggcgatt ttcggtgcc cccattgct ggggcgttt 
      accgaagcc actgggagc tacgatgct gggttttat 
      ctgtcgggg agtttgatc cttgcgtcg gcggttatg 
      tgctatcct ttgaattat atcaatagg cgcgagatg 
      caaaagaaa aaagagatg gtggaaaat aaatcagtt 
      ccagtttaa
    mRNA sequence
    • >TC000459 RA
      cgcgccaag ttctgtgct ttcagtcaa ccagttagt 
      gttgaagtg aacgcggga aaaattcac tgcatgtgt 
      tcttgtgtg atatagttt ataggctta tgatttttt 
      ttctgcaaa gcaggtaaa ccgataaga catgtcgcc 
      cccgaacgg tgctcctcc aggaaccgc cgaaaccga 
      gtacctcaa agtgcccac cgaacccaa accgacccc 
      cgaagagca acaaccctt gaacgacct cgtccccga 
      ggacgatgc agaagtggt cgtgccccc cgacggggg 
      ctggggctg ggtcgtggt tttcgcctc tttcatgtg 
      caatatgat cgtcgatgg gatcatttt ctcgtttgg 
      ggctttcat cgagcccat caaagacga atttcacgt 
      cacgaaagc atatgtggc gcttgtggg gtctctcat 
      gtccgggtt ttatcttat cgctgggcc ctttgccag 
      cgctgtcgc caacagata tgggtttag actggtggc 
      catcatggg gagcgtctt gggggcggc ggccttcgc 
      cttggccaa cttcgcccc caacgtgga gtatctctg 
      cgtcatgtt tggcgttat aggagggat tggtttcgg 
      gtttatcta cgtgccgtc gatcatcac cgttgggtt 
      ttatttcga gaaatggcg ggccttggc gaccggaat 
      cggggtttg cggttccgg tatcggaac ctttatgtt 
      tgcccccat aacagcata cctgatcga gaaccttgg 
      ttggagaac tgcgatttt gtgccaagg cactattgt 
      gttcgcttg tggcagttt tgggttcct ctacaggcc 
      gttaaaacc gacaaaagt ccatgatat taagggtga 
      gggagagac tgaacttaa aatggatcc gagcaaact 
      gccccccat tactaagat gaagatgga gctggctct 
      cgaagccat gaaaagtaa tgagtcttg tcacaacag 
      ccaagcctc aatcaaccg catgctggg ggttaacaa 
      caacgcgaa ctacccccg agtctcgga cgtgtacca 
      tacgattag cattccgca gcaaagggc cctggacgg 
      gtccagtta cgagaaacg gttgagcgt gccgtttat 
      ttccgaagc cgatcggat gaaaaacac aggagcgaa 
      ggaatgctt gctccagaa gagtgcgaa cccgattat 
      agttcctaa aggttcaag acggggttc tgcggttga 
      ccccgaggt taatcgacc aatgtaccg agacgatat 
      cttcttcaa cgcttcctt aacacgttt acctcaata 
      cacctctcg gacatcaat cgcctacaa cttatcagt 
      cacgcggtt accgacgaa aaacgacat cgaggaaga 
      gaagaaaag ttcttgcaa aatgtgccc agaagccgt 
      caaacgaac tctcgccac aatgctgga ctacagtct 
      actttgctc cccaacctt cctcttgct agcccttgg 
      cggcttctt caccatgat gggtttcta cgtcccgtt 
      catgttcct cgtaagccg ggccgagaa cgcgaacct 
      ggcaaaaga gtacacgag gtttctcat ctcagctat 
      tggcatttc caacacaat cgggcgtgt tttgtgcgg 
      cgtccttag ctctttccc cggtgttaa cgctctgtt 
      tgtgacaaa cgctgcgct gacgatcgg cggaatcgc 
      aacaatatt cagcggttt ttccatgac tgctgaata 
      ccaattctt ctacaccgc ggtgtttgg tttagctat 
      ttcaacctt tgcgtcgct gaggtcaat cgttgtggt 
      ggatcttct ggggctgga gaagttaac gaatgcgtt 
      tgggttgct tttgctgtt tcaaggagt ggcggcgat 
      tttcggtgc ccccattgc tggggcgtt taccgaagc 
      cactgggag ctacgatgc tgggtttta tctgtcggg 
      gagtttgat ccttgcgtc ggcggttat gtgctatcc 
      tttgaatta tatcaatag gcgcgagat gcaaaagaa 
      aaaagagat ggtggaaaa taaatcagt tccagttta 
      atgattatt tattttata aataatcgt tttgaataa 
      ttcatgatt ttccacgtt cctatttaa atgggtgct 
      acgtcatag caactatgt attgagttg tttgtcagg 
      ggattctta atactcgaa tttgtgcgc ttggtagtt 
      ctcgcgtac tttaactat aatgacagg gtttctata 
      cgacgattt ttcaagact gatgtagtt ttaagacct 
      gtacatatt gtatttaaa actgtaatt taacataat 
      ggaaaatta tggaattta cctaattag tttagattt 
      tataattgc tgaacgatt atttatgac agctgttct 
      tgttggaca ttccaatgt ttcattacc attaggagg 
      aattttcct atattgtac gtaaattat tatactcta 
      gctatagac caaattgtt aatttgata caaacggaa 
      ctttaattg atttgtcaa agtaaaata aaatttgtt 
      gttttga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY