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Gene information
General information
TC000444 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 5689379..5691547
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000444
    iBeetle screen iB_00095
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    thorax complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen dorsal irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000444 RA
      MDVQIILEG PPFPPNYHM KQSLGQFFF DSASKFKDR 
      ICQIDAKTE KSETFLTVK QKSVRVALE MQKRGITSK 
      DVIVTCSAL TLETPVPIL ASFYLGAKV ANSDPTLSV 
      AQTAHMLSL VSPTMIFVQ ESSLTLIEE SLQQAKLQA 
      QIVVFGTCD KYPTFSDFN QAKENEEMF YPASVDIHD 
      TGLMFFSSG TTGLPKAIC HSHFSFLNL AYCFCESGL 
      KVDTTLSYT TFYWISGLM MLTSSFLKG GQRIVCTKV 
      PEAKDVFEI IQKYKVTCL FLAPVLTYP LTDYANSGN 
      FDTSSLHSI LTGGTPISE QQMTKLYAT FKHTCIVFA 
      YGMTEVGMV GMFNPVTDK VLMTKKLTS SGKVAMGVS 
      LKLVDVETN KTLGPNQKG EFLVKSPCM MKGYYKADC 
      SDIFDEDGF LKTGDVGYY DQDGCLYIV ERRKEMFKY 
      LSWHIVPSA IENVLLEHP EIKEAAVFG MPINEEMGD 
      APAACVVLQ NGSKVTVQE IADFVASKV SDREKLRGG 
      VFIVQELPR TPSGKLKRR DVKNYVLSL NRGTNKQ
    CDS sequence
    • >TC000444 RA
      atggatgta caaataata ttagaaggt cccccattt 
      ccacccaat tatcacatg aaacaaagt ttgggtcaa 
      tttttcttt gattccgcc tcaaaattc aaagaccga 
      atatgccag atcgacgcc aaaaccgaa aaaagtgaa 
      acgttctta acagttaaa caaaaaagt gttcgagtc 
      gctttggag atgcagaaa cgaggaata acatcaaag 
      gatgtgatt gttacttgc tctgcgcta actttagag 
      accccagtc cccatcctt gcttctttt tacctcggt 
      gctaaagtg gcaaactca gacccgact ttatcagtc 
      gcacaaacg gctcacatg ctatcactt gtatcccca 
      acaatgatt ttcgtccaa gaaagctca ctcactctg 
      attgaagaa agcttgcaa caagccaag cttcaagca 
      cagattgtg gtttttggg acgtgtgac aagtatcca 
      acattttcc gatttcaac caagcgaag gaaaacgaa 
      gagatgttt tatccagcc tctgtcgat attcacgac 
      actggtctg atgttcttc agcagcggc acaacaggg 
      cttcccaaa gcgatctgt cacagtcat ttcagtttc 
      ttaaacctt gcttactgt ttttgtgaa tcggggctt 
      aaggttgac acgactctt tcttacact actttttac 
      tggatatcg gggcttatg atgttgact tcttcgttt 
      ttaaaaggc ggacagcga attgtttgc accaaggtg 
      cccgaagcc aaagacgtt tttgaaatt attcaaaaa 
      tataaagta acttgtctg tttttggct ccggtttta 
      acataccca ctcacagat tacgcaaac agcggcaat 
      tttgatact tcttcgctg cattctatt cttaccggt 
      ggtacccca ataagcgaa caacaaatg accaaactt 
      tacgccact tttaagcac acttgcatt gtatttgcg 
      tacggtatg acggaagtt ggcatggtt ggaatgttt 
      aatccagta actgataaa gttctaatg actaagaaa 
      ttaacttct tctggaaaa gtggcaatg ggggtctca 
      ctgaagcta gtggatgtt gaaaccaat aaaaccttg 
      ggcccgaac cagaaaggc gaatttctg gttaaatcc 
      ccttgcatg atgaagggc tattacaaa gccgattgt 
      tccgacatt ttcgacgaa gacggtttc ctaaagacg 
      ggagatgtt ggttattac gaccaagac gggtgtctt 
      tacattgta gaaagacgc aaagaaatg tttaaatat 
      ctgtcctgg cacattgtt ccatcggct atcgaaaat 
      gttttgctt gaacatccc gaaataaaa gaggctgca 
      gttttcgga atgccgata aatgaagaa atgggggac 
      gcacctgcg gcttgtgtg gtcttgcaa aatgggagt 
      aaagtcact gtgcaagaa attgcagat tttgtcgct 
      agcaaggtt tcagacagg gagaaatta agaggaggt 
      gtcttcata gtgcaagaa ctgcccagg acaccgagt 
      ggcaaattg aaaaggcga gacgttaaa aattacgtt 
      ttaagttta aatagggga acaaataaa caataa
    mRNA sequence
    • >TC000444 RA
      aaactacat caaacatgg atgtacaaa taatattag 
      aaggtcccc catttccac ccaattatc acatgaaac 
      aaagtttgg gtcaatttt tctttgatt ccgcctcaa 
      aattcaaag accgaatat gccagatcg acgccaaaa 
      ccgaaaaaa gtgaaacgt tcttaacag ttaaacaaa 
      aaagtgttc gagtcgctt tggagatgc agaaacgag 
      gaataacat caaaggatg tgattgtta cttgctctg 
      cgctaactt tagagaccc cagtcccca tccttgctt 
      ctttttacc tcggtgcta aagtggcaa actcagacc 
      cgactttat cagtcgcac aaacggctc acatgctat 
      cacttgtat ccccaacaa tgattttcg tccaagaaa 
      gctcactca ctctgattg aagaaagct tgcaacaag 
      ccaagcttc aagcacaga ttgtggttt ttgggacgt 
      gtgacaagt atccaacat tttccgatt tcaaccaag 
      cgaaggaaa acgaagaga tgttttatc cagcctctg 
      tcgatattc acgacactg gtctgatgt tcttcagca 
      gcggcacaa cagggcttc ccaaagcga tctgtcaca 
      gtcatttca gtttcttaa accttgctt actgttttt 
      gtgaatcgg ggcttaagg ttgacacga ctctttctt 
      acactactt tttactgga tatcggggc ttatgatgt 
      tgacttctt cgtttttaa aaggcggac agcgaattg 
      tttgcacca aggtgcccg aagccaaag acgtttttg 
      aaattattc aaaaatata aagtaactt gtctgtttt 
      tggctccgg ttttaacat acccactca cagattacg 
      caaacagcg gcaattttg atacttctt cgctgcatt 
      ctattctta ccggtggta ccccaataa gcgaacaac 
      aaatgacca aactttacg ccactttta agcacactt 
      gcattgtat ttgcgtacg gtatgacgg aagttggca 
      tggttggaa tgtttaatc cagtaactg ataaagttc 
      taatgacta agaaattaa cttcttctg gaaaagtgg 
      caatggggg tctcactga agctagtgg atgttgaaa 
      ccaataaaa ccttgggcc cgaaccaga aaggcgaat 
      ttctggtta aatcccctt gcatgatga agggctatt 
      acaaagccg attgttccg acattttcg acgaagacg 
      gtttcctaa agacgggag atgttggtt attacgacc 
      aagacgggt gtctttaca ttgtagaaa gacgcaaag 
      aaatgttta aatatctgt cctggcaca ttgttccat 
      cggctatcg aaaatgttt tgcttgaac atcccgaaa 
      taaaagagg ctgcagttt tcggaatgc cgataaatg 
      aagaaatgg gggacgcac ctgcggctt gtgtggtct 
      tgcaaaatg ggagtaaag tcactgtgc aagaaattg 
      cagattttg tcgctagca aggtttcag acagggaga 
      aattaagag gaggtgtct tcatagtgc aagaactgc 
      ccaggacac cgagtggca aattgaaaa ggcgagacg 
      ttaaaaatt acgttttaa gtttaaata ggggaacaa 
      ataaacaat aatacttca gaagtttgt gtttcttta 
      tcataaaaa ctaacatgt tttgccagg taattgaat 
      actgatgtt ttatgaaaa aataagtga tcttgtcgc 
      tgattattg attctcgtt caatatttt tatcactgt 
      caacacgtt tatcggagt atgagacaa gcaattaaa 
      tttataaaa tatgtctta ttaattttt attaaaatt 
      tgatgaagc acaacagcc gttcataaa ttttcctca 
      attaaatcc agaatggtt ggaattatt aattttttc 
      ctaattcaa ttagcaaca cccgccgta ctatttgtg 
      gaatcatgt caattggta ataaatatc ttattgtta 
      a