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Gene information
General information
TC000434 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11464362..11465520
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000434
    iBeetle screen iB_00093

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000434 RA
      MATLTRALQ KLALTSTQN APKVLTSVR YRHVPPPKP 
      EIGHGKSYR RIVHFPENY TVKPLDVTN LGGRDPETG 
      RLAVKGIGG GIKHKYHWI DWRRVGPSE GPPQTERVI 
      KIIKDGCRT AFVALVAHG DKLKYILAT ENMKPGDLI 
      KTSCHIPRI PVRANEGDA YPLGALPLG TQVHCIEKV 
      PGLGGFLVH TAGSFATIM RKVDNRVIV MMPSKKEFS 
      LLENCMCTV GRLSNVEHA TTPIGSAQK NRELGNRPR 
      SGWWQRKTG RFGRKLRRP PPVKTIGPK EDTNQEVVK 
      LSFDGYFCK TPRAQFHY
    CDS sequence
    • >TC000434 RA
      atggccact ttaacgcga gctttacaa aaattagcc 
      ttaacaagc actcaaaat gcgcctaaa gtcttgact 
      agcgtgaga taccgccat gtcccgccg ccaaaaccc 
      gaaattgga catgggaag agttaccga agaatcgtt 
      cacttcccc gaaaattac accgtcaag cctttggac 
      gtgaccaat ttggggggc agagacccc gagacgggc 
      cggctggct gttaagggc atcggcggg ggcataaaa 
      cacaagtac cactggatt gactggaga cgagtgggc 
      cccagtgag ggcccccca caaaccgaa cgagtcatt 
      aaaataatc aaggatggg tgcaggaca gctttcgtc 
      gcacttgta gcacatggt gacaaatta aaatatatt 
      ctagccacg gaaaacatg aaacctggg gatttaatc 
      aagacctcg tgtcacatc ccccgtatt ccagttcgt 
      gccaatgag ggtgatgcg taccccctt ggggcgctg 
      ccgctgggt actcaggtt cattgcatt gagaaagtc 
      ccaggtttg ggcggattt ttggtccac acggcgggg 
      tcttttgct acaataatg cggaaagtt gataataga 
      gttattgtt atgatgccg agtaaaaag gagtttagt 
      ttgctggag aactgcatg tgtactgtg ggtagatta 
      agtaatgtg gagcacgct acaactcct attggttca 
      gcgcaaaaa aatagagaa ttggggaac aggcctagg 
      tctggatgg tggcaacgc aaaactgga cgatttgga 
      cgaaagcta aggcggcca ccgcctgtt aaaactatc 
      gggcctaag gaagacacg aatcaagaa gtggtaaag 
      ttgagtttc gatggatat ttttgcaag acaccaagg 
      gctcagttt cattattaa
    mRNA sequence
    • >TC000434 RA
      gggcggtat taaaacact aattatgct tccgtagtt 
      cacaaaacc aacaacaat attcagaaa aattgcaat 
      tttatgcaa tttttgtaa cccggctaa cttcaagtc 
      ggttgcttt aaccttaat tgtcaaggg tttcctaac 
      cttaaatgg ccactttaa cgcgagctt tacaaaaat 
      tagccttaa caagcactc aaaatgcgc ctaaagtct 
      tgactagcg tgagatacc gccatgtcc cgccgccaa 
      aacccgaaa ttggacatg ggaagagtt accgaagaa 
      tcgttcact tccccgaaa attacaccg tcaagcctt 
      tggacgtga ccaatttgg ggggcagag accccgaga 
      cgggccggc tggctgtta agggcatcg gcgggggca 
      taaaacaca agtaccact ggattgact ggagacgag 
      tgggcccca gtgagggcc ccccacaaa ccgaacgag 
      tcattaaaa taatcaagg atgggtgca ggacagctt 
      tcgtcgcac ttgtagcac atggtgaca aattaaaat 
      atattctag ccacggaaa acatgaaac ctggggatt 
      taatcaaga cctcgtgtc acatccccc gtattccag 
      ttcgtgcca atgagggtg atgcgtacc cccttgggg 
      cgctgccgc tgggtactc aggttcatt gcattgaga 
      aagtcccag gtttgggcg gatttttgg tccacacgg 
      cggggtctt ttgctacaa taatgcgga aagttgata 
      atagagtta ttgttatga tgccgagta aaaaggagt 
      ttagtttgc tggagaact gcatgtgta ctgtgggta 
      gattaagta atgtggagc acgctacaa ctcctattg 
      gttcagcgc aaaaaaata gagaattgg ggaacaggc 
      ctaggtctg gatggtggc aacgcaaaa ctggacgat 
      ttggacgaa agctaaggc ggccaccgc ctgttaaaa 
      ctatcgggc ctaaggaag acacgaatc aagaagtgg 
      taaagttga gtttcgatg gatattttt gcaagacac 
      caagggctc agtttcatt attaattaa tttggatgt 
      cggagtttc gaaataaat tgatttatt gagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY