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Gene information
General information
TC000423 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11558485..11559788
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000423
    iBeetle screen iB_00090

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    remarks: animals lost, low validity due to technical reasons - should be repeated: stink gland phenotype not scorable, adult phenotype not scorable, lethality not scorable,
    adult progeny in the well
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost, low validity due to technical reasons - should be repeated: stink gland phenotype not scorable, adult phenotype not scorable, lethality not scorable,
    adult progeny in the well
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000423 RA
      MNSLKVLIF LPLLVLVNC DCGQNQTVK LNVTVFQDI 
DSVGENLTV QSPFEYKKD IFHLLIVNE TLPEVCENF 
FLVQNELSI LQIVNSSVQ TIHEGAFNV TPTLALIRI 
VQNQINTIP KNVFNNIKV KEIDLSQNL ISTIETEAF 
DNNTYLEIV KLNNNQIKE INPNWFLNS PKVYKLSAV 
YNDIKAIPA EAFKNMDQT RPLKLRFSA NRITEINPD 
AFNSHHTIQ LLRINGNKL TTLPENIFI NRTIQNLQV 
NTNQLQCFP DVMFESGLT KLTFIDNIS FECSCLKKV 
RKYAEDNKL EVWYPSIIC EDRTREVNI VFNYNKTYE 
IPLIPPTVD VDVYVKPDQ PT
    CDS sequence
    • >TC000423 RA
      atgaacagc ctgaaagta ttaattttt ttaccatta 
ctagtgtta gtgaattgt gattgtggt caaaatcaa 
acagtcaag ctaaacgtg acggtgttc caagacatt 
gacagtgtc ggtgaaaac ctaacagtg cagagcccc 
ttcgagtat aaaaaagac attttccac ttgctgatc 
gttaacgaa actttgcca gaagtgtgt gaaaatttc 
ttcttagtc caaaacgaa ttgtccatt ttgcaaatc 
gtcaacagc agcgtacaa acaattcat gaaggcgct 
ttcaacgta acgccaact ctagctcta atacgaatc 
gtccaaaac caaattaac acaatccca aaaaacgta 
ttcaacaat attaaagtc aaggaaatt gatttatcc 
caaaatctg atatcaaca atcgaaacg gaagccttt 
gacaacaac acgtattta gaaatcgtg aaactcaac 
aacaaccaa ataaaggaa atcaaccca aattggttc 
ctaaacagc cccaaagtg tataaattg agcgctgtt 
tacaacgac atcaaagcc atacctgcg gaagctttc 
aaaaacatg gaccaaaca aggcctctg aaactgagg 
ttttcagca aataggatc actgaaatt aatccggac 
gctttcaac agtcaccac accatccag ttattgaga 
atcaacgga aacaaactg actacactc ccggaaaac 
attttcata aaccggact attcaaaat ctgcaagtc 
aacacaaac caactgcag tgcttccca gacgtcatg 
tttgagagc ggattaact aaactaacg tttatagac 
aatatatct ttcgagtgt tcctgtttg aagaaggtg 
cggaaatac gccgaagac aacaaactg gaggtctgg 
tatccgtcc ataatctgc gaggaccgg accagagaa 
gtgaatatt gttttcaat tacaacaaa acatacgaa 
ataccccta ataccacca actgttgac gtcgacgtc 
tacgtcaag cctgatcaa ccaacgtaa
    mRNA sequence
    • >TC000423 RA
      aaccgttat tgtttcccg tgttttaaa agtctacgg 
acgctgtaa ttgcaaatc taaatataa atcatgtct 
tcgagtaag atttaaaat tagctcctt tgatacgtt 
atcacgggg gttatccga gccgttgaa cttcgacac 
tgattgtaa agtacatgt ttattaaca aatcatgtt 
caaaattag acggacgta ctaacgttc caaaatgaa 
cagcctgaa agtattaat ttttttacc attactagt 
gttagtgaa ttgtgattg tggtcaaaa tcaaacagt 
caagctaaa cgtgacggt gttccaaga cattgacag 
tgtcggtga aaacctaac agtgcagag ccccttcga 
gtataaaaa agacatttt ccacttgct gatcgttaa 
cgaaacttt gccagaagt gtgtgaaaa tttcttctt 
agtccaaaa cgaattgtc cattttgca aatcgtcaa 
cagcagcgt acaaacaat tcatgaagg cgctttcaa 
cgtaacgcc aactctagc tctaatacg aatcgtcca 
aaaccaaat taacacaat cccaaaaaa cgtattcaa 
caatattaa agtcaagga aattgattt atcccaaaa 
tctgatatc aacaatcga aacggaagc ctttgacaa 
caacacgta tttagaaat cgtgaaact caacaacaa 
ccaaataaa ggaaatcaa cccaaattg gttcctaaa 
cagccccaa agtgtataa attgagcgc tgtttacaa 
cgacatcaa agccatacc tgcggaagc tttcaaaaa 
catggacca aacaaggcc tctgaaact gaggttttc 
agcaaatag gatcactga aattaatcc ggacgcttt 
caacagtca ccacaccat ccagttatt gagaatcaa 
cggaaacaa actgactac actcccgga aaacatttt 
cataaaccg gactattca aaatctgca agtcaacac 
aaaccaact gcagtgctt cccagacgt catgtttga 
gagcggatt aactaaact aacgtttat agacaatat 
atctttcga gtgttcctg tttgaagaa ggtgcggaa 
atacgccga agacaacaa actggaggt ctggtatcc 
gtccataat ctgcgagga ccggaccag agaagtgaa 
tattgtttt caattacaa caaaacata cgaaatacc 
cctaatacc accaactgt tgacgtcga cgtctacgt 
caagcctga tcaaccaac gtaaagata aggcacaac 
aggatttga atttgtaca cttattaat aaatgattc 
cacagtaa
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