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Gene information
General information
TC000423 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 6051942..6053245
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000423
    iBeetle screen iB_00090
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    remarks: animals lost, low validity due to technical reasons - should be repeated: stink gland phenotype not scorable, adult phenotype not scorable, lethality not scorable,
    adult progeny in the well
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost, low validity due to technical reasons - should be repeated: stink gland phenotype not scorable, adult phenotype not scorable, lethality not scorable,
    adult progeny in the well
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000423 RA
      MNSLKVLIF LPLLVLVNC DCGQNQTVK LNVTVFQDI 
      DSVGENLTV QSPFEYKKD IFHLLIVNE TLPEVCENF 
      FLVQNELSI LQIVNSSVQ TIHEGAFNV TPTLALIRI 
      VQNQINTIP KNVFNNIKV KEIDLSQNL ISTIETEAF 
      DNNTYLEIV KLNNNQIKE INPNWFLNS PKVYKLSAV 
      YNDIKAIPA EAFKNMDQT RPLKLRFSA NRITEINPD 
      AFNSHHTIQ LLRINGNKL TTLPENIFI NRTIQNLQV 
      NTNQLQCFP DVMFESGLT KLTFIDNIS FECSCLKKV 
      RKYAEDNKL EVWYPSIIC EDRTREVNI VFNYNKTYE 
      IPLIPPTVD VDVYVKPDQ PT
    CDS sequence
    • >TC000423 RA
      atgaacagc ctgaaagta ttaattttt ttaccatta 
      ctagtgtta gtgaattgt gattgtggt caaaatcaa 
      acagtcaag ctaaacgtg acggtgttc caagacatt 
      gacagtgtc ggtgaaaac ctaacagtg cagagcccc 
      ttcgagtat aaaaaagac attttccac ttgctgatc 
      gttaacgaa actttgcca gaagtgtgt gaaaatttc 
      ttcttagtc caaaacgaa ttgtccatt ttgcaaatc 
      gtcaacagc agcgtacaa acaattcat gaaggcgct 
      ttcaacgta acgccaact ctagctcta atacgaatc 
      gtccaaaac caaattaac acaatccca aaaaacgta 
      ttcaacaat attaaagtc aaggaaatt gatttatcc 
      caaaatctg atatcaaca atcgaaacg gaagccttt 
      gacaacaac acgtattta gaaatcgtg aaactcaac 
      aacaaccaa ataaaggaa atcaaccca aattggttc 
      ctaaacagc cccaaagtg tataaattg agcgctgtt 
      tacaacgac atcaaagcc atacctgcg gaagctttc 
      aaaaacatg gaccaaaca aggcctctg aaactgagg 
      ttttcagca aataggatc actgaaatt aatccggac 
      gctttcaac agtcaccac accatccag ttattgaga 
      atcaacgga aacaaactg actacactc ccggaaaac 
      attttcata aaccggact attcaaaat ctgcaagtc 
      aacacaaac caactgcag tgcttccca gacgtcatg 
      tttgagagc ggattaact aaactaacg tttatagac 
      aatatatct ttcgagtgt tcctgtttg aagaaggtg 
      cggaaatac gccgaagac aacaaactg gaggtctgg 
      tatccgtcc ataatctgc gaggaccgg accagagaa 
      gtgaatatt gttttcaat tacaacaaa acatacgaa 
      ataccccta ataccacca actgttgac gtcgacgtc 
      tacgtcaag cctgatcaa ccaacgtaa
    mRNA sequence
    • >TC000423 RA
      aaccgttat tgtttcccg tgttttaaa agtctacgg 
      acgctgtaa ttgcaaatc taaatataa atcatgtct 
      tcgagtaag atttaaaat tagctcctt tgatacgtt 
      atcacgggg gttatccga gccgttgaa cttcgacac 
      tgattgtaa agtacatgt ttattaaca aatcatgtt 
      caaaattag acggacgta ctaacgttc caaaatgaa 
      cagcctgaa agtattaat ttttttacc attactagt 
      gttagtgaa ttgtgattg tggtcaaaa tcaaacagt 
      caagctaaa cgtgacggt gttccaaga cattgacag 
      tgtcggtga aaacctaac agtgcagag ccccttcga 
      gtataaaaa agacatttt ccacttgct gatcgttaa 
      cgaaacttt gccagaagt gtgtgaaaa tttcttctt 
      agtccaaaa cgaattgtc cattttgca aatcgtcaa 
      cagcagcgt acaaacaat tcatgaagg cgctttcaa 
      cgtaacgcc aactctagc tctaatacg aatcgtcca 
      aaaccaaat taacacaat cccaaaaaa cgtattcaa 
      caatattaa agtcaagga aattgattt atcccaaaa 
      tctgatatc aacaatcga aacggaagc ctttgacaa 
      caacacgta tttagaaat cgtgaaact caacaacaa 
      ccaaataaa ggaaatcaa cccaaattg gttcctaaa 
      cagccccaa agtgtataa attgagcgc tgtttacaa 
      cgacatcaa agccatacc tgcggaagc tttcaaaaa 
      catggacca aacaaggcc tctgaaact gaggttttc 
      agcaaatag gatcactga aattaatcc ggacgcttt 
      caacagtca ccacaccat ccagttatt gagaatcaa 
      cggaaacaa actgactac actcccgga aaacatttt 
      cataaaccg gactattca aaatctgca agtcaacac 
      aaaccaact gcagtgctt cccagacgt catgtttga 
      gagcggatt aactaaact aacgtttat agacaatat 
      atctttcga gtgttcctg tttgaagaa ggtgcggaa 
      atacgccga agacaacaa actggaggt ctggtatcc 
      gtccataat ctgcgagga ccggaccag agaagtgaa 
      tattgtttt caattacaa caaaacata cgaaatacc 
      cctaatacc accaactgt tgacgtcga cgtctacgt 
      caagcctga tcaaccaac gtaaagata aggcacaac 
      aggatttga atttgtaca cttattaat aaatgattc 
      cacagtaa