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Gene information
General information
TC000415 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 6255729..6258886
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000415
    iBeetle screen iB_00089
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000415 RA
      MEKTGGKFV LEWETHSKQ ISRGLCMLM ERQCLVDIA 
      VCCGSNTLH AHKCVLAAS SSYFKEHLE NKAIEQVVI 
      NGLDFAVMK SLIEFMYSG ECAFSEDHL KYFIAAVKF 
      FKITALESI FAENEYQNI SADEVCIPP PQFICKPPK 
      YPTVYKPFS NASLPPKIN YALSNVYGQ HKFKRKCLG 
      SEAEKACAK EAQASRIAL ANLRKVMAS TPQVHSFIV 
      EESFNETTV ENFIPNVEE NFVENIHDV QFVNGNAPP 
      ATANGKPLS VIPVSNTQS LTADKIKHI LGNDEVEIM 
      FQTSDGNFV NVSDEVLLN LSKGGLQYQ VVDENGHIG 
      EIQELKTLD SENGPKDDQ DLKTETFDL KIIKNPDEI 
      DKIAKAMPE EYIDPNFSS AACNKTNME SLQLHTSFN 
      EDLYENSFH MQSAEMFEP ENLSMHSDL MADNAMEFE 
      SDLKYKYLT SKINNTHDT INFDEHIIT KIDSMSEDP 
      LRDTNEVLV METDTTKSP QNKSSLKFT PDMFFTTLP 
      ANACTEDKS KIPAVNDVA E
    CDS sequence
    • >TC000415 RA
      atggagaaa actggtgga aagttcgtt ttagaatgg 
      gaaactcat tcaaaacag atctccaga ggcttgtgt 
      atgttgatg gagaggcaa tgtctagtg gatattgcc 
      gtctgttgt gggagtaat acgttgcat gcgcataaa 
      tgtgtattg gccgccagt agtagctat tttaaagag 
      catcttgaa aataaggct attgaacaa gttgtaata 
      aatggctta gatttcgct gtcatgaag tcattaatt 
      gaatttatg tactcgggg gaatgcgct ttttctgag 
      gatcatctc aagtacttt attgccgct gttaagttt 
      ttcaagatc actgcgttg gaaagcatt tttgccgaa 
      aatgaatac caaaatata tcagcggat gaagtgtgc 
      ataccccct ccgcaattt atatgtaaa ccccccaag 
      tacccaacc gtttacaaa cctttttcc aacgcatcc 
      ctgcctcca aaaataaat tatgcttta agtaacgtg 
      tacgggcaa cataaattc aaacgaaag tgccttggg 
      agcgaagct gaaaaagca tgcgccaaa gaggcccaa 
      gcgagtcgc attgctttg gctaatttg cgaaaagtt 
      atggcgagc acaccgcaa gttcactcc tttatcgtc 
      gaggagtct ttcaatgaa acgaccgtc gagaatttt 
      atccctaac gtagaggag aacttcgtt gaaaacatt 
      cacgacgtg caatttgtt aatggaaac gcgccccca 
      gcgaccgcc aacggcaaa cccctcagc gttatcccg 
      gtctccaac acccagagc ctcaccgcg gacaagatc 
      aagcacatt ttgggcaac gacgaggtg gaaattatg 
      ttccaaacg agcgacggc aacttcgtc aacgtctcc 
      gacgaggtt ttgctcaac ttgtcgaaa ggggggctc 
      cagtaccag gtggtcgac gaaaacggc cacattggc 
      gagatccag gagctgaag acccttgac agcgagaat 
      ggccctaaa gacgaccag gatttaaaa acggagacc 
      ttcgacctc aaaatcatt aaaaatcca gacgaaatc 
      gataaaatc gccaaagca atgcccgaa gaatacatt 
      gacccgaac ttttccagt gccgcttgt aataaaacc 
      aacatggag tcgctccaa ttgcacaca agttttaac 
      gaggatctg tacgaaaac agttttcac atgcaaagt 
      gcggaaatg ttcgagccg gaaaatctt agcatgcat 
      tcagatttg atggcggat aatgcaatg gagtttgag 
      agcgatttg aaatacaaa tacttgact tcgaaaatt 
      aataacact cacgatact atcaacttt gacgagcac 
      atcataaca aagatcgac agtatgagc gaagatcct 
      ttgcgtgat actaatgaa gtgttggtt atggagacg 
      gatacgacg aaaagccca cagaataaa tcatctttg 
      aagtttaca ccggacatg ttttttacc acgctacca 
      gctaatgcg tgtactgaa gataaatcg aagattcct 
      gctgtgaat gacgttgcg gaatag
    mRNA sequence
    • >TC000415 RA
      gggcgaaaa aattttctt ttggggttg caaatcaag 
      tacgtaagt gtttggctt aataatatt tatatttgc 
      ctcattaat ttctcgtaa aattattaa aaagtgagg 
      tcggtggtg ttaaagaat gtttgtggt caagtgtta 
      ggtgttaag agtgaagat tagtagcac ttgcgacca 
      gtttttgcg gatccaagc cttattaga gttttcgtg 
      actgaaaat ggagaaaac tggtggaaa gttcgtttt 
      agaatggga aactcattc aaaacagat ctccagagg 
      cttgtgtat gttgatgga gaggcaatg tctagtgga 
      tattgccgt ctgttgtgg gagtaatac gttgcatgc 
      gcataaatg tgtattggc cgccagtag tagctattt 
      taaagagca tcttgaaaa taaggctat tgaacaagt 
      tgtaataaa tggcttaga tttcgctgt catgaagtc 
      attaattga atttatgta ctcggggga atgcgcttt 
      ttctgagga tcatctcaa gtactttat tgccgctgt 
      taagttttt caagatcac tgcgttgga aagcatttt 
      tgccgaaaa tgaatacca aaatatatc agcggatga 
      agtgtgcat accccctcc gcaatttat atgtaaacc 
      ccccaagta cccaaccgt ttacaaacc tttttccaa 
      cgcatccct gcctccaaa aataaatta tgctttaag 
      taacgtgta cgggcaaca taaattcaa acgaaagtg 
      ccttgggag cgaagctga aaaagcatg cgccaaaga 
      ggcccaagc gagtcgcat tgctttggc taatttgcg 
      aaaagttat ggcgagcac accgcaagt tcactcctt 
      tatcgtcga ggagtcttt caatgaaac gaccgtcga 
      gaattttat ccctaacgt agaggagaa cttcgttga 
      aaacattca cgacgtgca atttgttaa tggaaacgc 
      gcccccagc gaccgccaa cggcaaacc cctcagcgt 
      tatcccggt ctccaacac ccagagcct caccgcgga 
      caagatcaa gcacatttt gggcaacga cgaggtgga 
      aattatgtt ccaaacgag cgacggcaa cttcgtcaa 
      cgtctccga cgaggtttt gctcaactt gtcgaaagg 
      ggggctcca gtaccaggt ggtcgacga aaacggcca 
      cattggcga gatccagga gctgaagac ccttgacag 
      cgagaatgg ccctaaaga cgaccagga tttaaaaac 
      ggagacctt cgacctcaa aatcattaa aaatccaga 
      cgaaatcga taaaatcgc caaagcaat gcccgaaga 
      atacattga cccgaactt ttccagtgc cgcttgtaa 
      taaaaccaa catggagtc gctccaatt gcacacaag 
      ttttaacga ggatctgta cgaaaacag ttttcacat 
      gcaaagtgc ggaaatgtt cgagccgga aaatcttag 
      catgcattc agatttgat ggcggataa tgcaatgga 
      gtttgagag cgatttgaa atacaaata cttgacttc 
      gaaaattaa taacactca cgatactat caactttga 
      cgagcacat cataacaaa gatcgacag tatgagcga 
      agatccttt gcgtgatac taatgaagt gttggttat 
      ggagacgga tacgacgaa aagcccaca gaataaatc 
      atctttgaa gtttacacc ggacatgtt ttttaccac 
      gctaccagc taatgcgtg tactgaaga taaatcgaa 
      gattcctgc tgtgaatga cgttgcgga atagttttt 
      tattgcttg gatgccgtt tttattatg ttttcattt 
      ggttgcaca ttctagtta gaagccctt gttattatg 
      accttttcg aattaactt aatataggg atattaaag 
      ctaattcaa gcgt