General information | |||
TC000412 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 11817328..11820089 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC000412 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00088 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (3 dpi)egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 62.5% (includes death as larva, prepupa, pupa, adult)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (22 dpi)
adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
low validity due to technical reasons - should be repeated: stink gland phenotype not scorable, adult phenotype not scorable, lethality not scorable,
adult progeny in the well |
motility decreased - (22 dpi)
low validity due to technical reasons - should be repeated: stink gland phenotype not scorable, adult phenotype not scorable, lethality not scorable,
adult progeny in the well |
- >TC000412 RA
MNAGYYAHS SPSRESGVV SDCDTMAEL AALLRSEIP EGRSNLTDN HTNLERVAE YCEANYFQS NNKRVALEE TKNYTTQSL ASVAYQINT LAFNFLQLL ELQAAQLAE MESQMNHIS QTVMIHKEK VARREIGVL TANKSTSRQ YKIIAPANP EKPIKYLRK PIDYNIFDD IGHGVRSSG TPRQQKQRG SSQGSIQSL TANPMVGPA PTTKPPTPP QVSRTSSKV SVSGGTLSK GSREYRTPP AVAPPQVPS HYAPNYPIG HARRSTERG PGYSTLPMA PGQHQAMSP PQVGMVHPM SINQQQHQN QQHPQTPPP PPPPGNVGN YIQAEHHMS MPPPPSPLI SQEMQMPSN QHHPMMMTQ HAPVPHGSL GMHTLSHAH GQRLARSSG AHSPPLPPP PPPEDEHEA FGRPRTSGV MPIVPDEED LPGWVPKNY IEKVVAIYD YYADKDDEL SFQESSVIY VLKKNDDGW WEGVMDGIT GLFPGNYVE PCV
- >TC000412 RA
atgaatgct ggatactac gcccacagc agccccagt cgagaatct ggagttgta agtgactgt gacacaatg gccgaactt gcagccttg ctgcgctcc gaaatccca gaggggcgc agcaacctg accgacaac cacacgaac ctggaaagg gttgccgaa tattgcgaa gctaactat ttccagtcg aataacaag cgcgtggct ttggaagaa accaaaaac tacacaaca cagtctttg gcaagtgtg gcctaccag atcaacaca ctcgctttc aattttctg caactgttg gagttgcaa gcggcacag ctggccgag atggagagc cagatgaac cacatctcg caaacggtg atgatccac aaggagaag gttgcaagg cgcgagatt ggagtgttg acggccaac aagtcaacc agccgacag tataaaatc atcgcgcct gctaacccg gagaaaccc atcaagtac ctccggaaa ccgattgac tacaacatt ttcgatgat atcgggcat ggggtgcgg agcagtggg acccccaga cagcaaaaa cagaggggt tccagtcag ggtagcatc cagtcgctc acggccaat cccatggtg ggcccagca cccaccacc aaaccgcca acgcctccg caagtatca agaacatca agtaaagtt tctgtttcg ggaggtacg ttgagtaaa ggcagtcgc gaatatcgt actcctcca gcggtggcg cctcctcag gttcctagt cactacgcc cccaattat cccattggc catgcaaga cgttcgacc gaaaggggg cccgggtac agcactttg cccatggct ccaggtcaa catcaggcg atgtcgcca ccacaagtt ggcatggta catcccatg agtataaat caacagcaa caccaaaac caacaacat cctcaaaca ccaccgccg ccgcctccg cccggtaat gtgggaaac tacatccaa gcggagcac catatgagc atgccgccg ccgccttcg ccgttgatc agccaagaa atgcaaatg ccgtcgaac cagcaccac ccgatgatg atgacccag catgcccca gtgccgcat ggaagcctg ggaatgcac actttgtcc catgcccat ggccaacgg cttgcccga agtagcggt gcacattcg cctccattg cctcctcca ccgccgcca gaagacgaa cacgaggct tttggcaga cctcgtacg agtggagtc atgcccatt gtgcccgat gaggaggat ttgcccgga tgggtaccc aagaattat atagagaaa gttgtggcg atttacgat tattacgct gataaagat gatgaattg agtttccaa gaaagttcg gttatttat gtgctgaag aaaaatgat gatggttgg tgggaaggt gtcatggat ggaattacg ggattgttt cctgggaat tacgtggaa ccttgtgta taa
- >TC000412 RA
atacgttgc tatcttgtc atccggttg acatttata atttccaaa atcgttttt tttaatcca gtgaaaaat tttgtgtac gtaaacata aattacgtt cctcttaat agtttcctt gtgttgcaa cttcgccta tttcaacca atactatgc taactccga ttttagtgt ttgcctctt gtctgttat gaatgctgg atactacgc ccacagcag ccccagtcg agaatctgg agttgtaag tgactgtga cacaatggc cgaacttgc agccttgct gcgctccga aatcccaga ggggcgcag caacctgac cgacaacca cacgaacct ggaaagggt tgccgaata ttgcgaagc taactattt ccagtcgaa taacaagcg cgtggcttt ggaagaaac caaaaacta cacaacaca gtctttggc aagtgtggc ctaccagat caacacact cgctttcaa ttttctgca actgttgga gttgcaagc ggcacagct ggccgagat ggagagcca gatgaacca catctcgca aacggtgat gatccacaa ggagaaggt tgcaaggcg cgagattgg agtgttgac ggccaacaa gtcaaccag ccgacagta taaaatcat cgcgcctgc taacccgga gaaacccat caagtacct ccggaaacc gattgacta caacatttt cgatgatat cgggcatgg ggtgcggag cagtgggac ccccagaca gcaaaaaca gaggggttc cagtcaggg tagcatcca gtcgctcac ggccaatcc catggtggg cccagcacc caccaccaa accgccaac gcctccgca agtatcaag aacatcaag taaagtttc tgtttcggg aggtacgtt gagtaaagg cagtcgcga atatcgtac tcctccagc ggtggcgcc tcctcaggt tcctagtca ctacgcccc caattatcc cattggcca tgcaagacg ttcgaccga aagggggcc cgggtacag cactttgcc catggctcc aggtcaaca tcaggcgat gtcgccacc acaagttgg catggtaca tcccatgag tataaatca acagcaaca ccaaaacca acaacatcc tcaaacacc accgccgcc gcctccgcc cggtaatgt gggaaacta catccaagc ggagcacca tatgagcat gccgccgcc gccttcgcc gttgatcag ccaagaaat gcaaatgcc gtcgaacca gcaccaccc gatgatgat gacccagca tgccccagt gccgcatgg aagcctggg aatgcacac tttgtccca tgcccatgg ccaacggct tgcccgaag tagcggtgc acattcgcc tccattgcc tcctccacc gccgccaga agacgaaca cgaggcttt tggcagacc tcgtacgag tggagtcat gcccattgt gcccgatga ggaggattt gcccggatg ggtacccaa gaattatat agagaaagt tgtggcgat ttacgatta ttacgctga taaagatga tgaattgag tttccaaga aagttcggt tatttatgt gctgaagaa aaatgatga tggttggtg ggaaggtgt catggatgg aattacggg attgtttcc tgggaatta cgtggaacc ttgtgtata atttactaa ttaggtttc cgcacgtaa taattatgc aaatcaatt attaatgtt attaagtgt aacccgact ttaaagaaa attcttcaa agtgtctag aatgaaact ttgtgtaga tgttttaca gttctgtag acggtatga tggtaattg tataattta tattatttt tctccaaac gtttgtaat acatttata agtaataaa aattctata tcata
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
