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Gene information
General information
TC000412 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11817328..11820089
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000412
    iBeetle screen iB_00088

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 62.5% (includes death as larva, prepupa, pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    low validity due to technical reasons - should be repeated: stink gland phenotype not scorable, adult phenotype not scorable, lethality not scorable,
    adult progeny in the well
    Pupal morphology
    elytra partially blistered - Number of animals showing the phenotype: 3

    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 5 (22 dpi)
    Days post injection



    motility decreased - (22 dpi)
    Days post injection


    low validity due to technical reasons - should be repeated: stink gland phenotype not scorable, adult phenotype not scorable, lethality not scorable,
    adult progeny in the well
    Fertility
    egg production: number no/almost no eggs -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000412 RA
      MNAGYYAHS SPSRESGVV SDCDTMAEL AALLRSEIP 
      EGRSNLTDN HTNLERVAE YCEANYFQS NNKRVALEE 
      TKNYTTQSL ASVAYQINT LAFNFLQLL ELQAAQLAE 
      MESQMNHIS QTVMIHKEK VARREIGVL TANKSTSRQ 
      YKIIAPANP EKPIKYLRK PIDYNIFDD IGHGVRSSG 
      TPRQQKQRG SSQGSIQSL TANPMVGPA PTTKPPTPP 
      QVSRTSSKV SVSGGTLSK GSREYRTPP AVAPPQVPS 
      HYAPNYPIG HARRSTERG PGYSTLPMA PGQHQAMSP 
      PQVGMVHPM SINQQQHQN QQHPQTPPP PPPPGNVGN 
      YIQAEHHMS MPPPPSPLI SQEMQMPSN QHHPMMMTQ 
      HAPVPHGSL GMHTLSHAH GQRLARSSG AHSPPLPPP 
      PPPEDEHEA FGRPRTSGV MPIVPDEED LPGWVPKNY 
      IEKVVAIYD YYADKDDEL SFQESSVIY VLKKNDDGW 
      WEGVMDGIT GLFPGNYVE PCV
    CDS sequence
    • >TC000412 RA
      atgaatgct ggatactac gcccacagc agccccagt 
      cgagaatct ggagttgta agtgactgt gacacaatg 
      gccgaactt gcagccttg ctgcgctcc gaaatccca 
      gaggggcgc agcaacctg accgacaac cacacgaac 
      ctggaaagg gttgccgaa tattgcgaa gctaactat 
      ttccagtcg aataacaag cgcgtggct ttggaagaa 
      accaaaaac tacacaaca cagtctttg gcaagtgtg 
      gcctaccag atcaacaca ctcgctttc aattttctg 
      caactgttg gagttgcaa gcggcacag ctggccgag 
      atggagagc cagatgaac cacatctcg caaacggtg 
      atgatccac aaggagaag gttgcaagg cgcgagatt 
      ggagtgttg acggccaac aagtcaacc agccgacag 
      tataaaatc atcgcgcct gctaacccg gagaaaccc 
      atcaagtac ctccggaaa ccgattgac tacaacatt 
      ttcgatgat atcgggcat ggggtgcgg agcagtggg 
      acccccaga cagcaaaaa cagaggggt tccagtcag 
      ggtagcatc cagtcgctc acggccaat cccatggtg 
      ggcccagca cccaccacc aaaccgcca acgcctccg 
      caagtatca agaacatca agtaaagtt tctgtttcg 
      ggaggtacg ttgagtaaa ggcagtcgc gaatatcgt 
      actcctcca gcggtggcg cctcctcag gttcctagt 
      cactacgcc cccaattat cccattggc catgcaaga 
      cgttcgacc gaaaggggg cccgggtac agcactttg 
      cccatggct ccaggtcaa catcaggcg atgtcgcca 
      ccacaagtt ggcatggta catcccatg agtataaat 
      caacagcaa caccaaaac caacaacat cctcaaaca 
      ccaccgccg ccgcctccg cccggtaat gtgggaaac 
      tacatccaa gcggagcac catatgagc atgccgccg 
      ccgccttcg ccgttgatc agccaagaa atgcaaatg 
      ccgtcgaac cagcaccac ccgatgatg atgacccag 
      catgcccca gtgccgcat ggaagcctg ggaatgcac 
      actttgtcc catgcccat ggccaacgg cttgcccga 
      agtagcggt gcacattcg cctccattg cctcctcca 
      ccgccgcca gaagacgaa cacgaggct tttggcaga 
      cctcgtacg agtggagtc atgcccatt gtgcccgat 
      gaggaggat ttgcccgga tgggtaccc aagaattat 
      atagagaaa gttgtggcg atttacgat tattacgct 
      gataaagat gatgaattg agtttccaa gaaagttcg 
      gttatttat gtgctgaag aaaaatgat gatggttgg 
      tgggaaggt gtcatggat ggaattacg ggattgttt 
      cctgggaat tacgtggaa ccttgtgta taa
    mRNA sequence
    • >TC000412 RA
      atacgttgc tatcttgtc atccggttg acatttata 
      atttccaaa atcgttttt tttaatcca gtgaaaaat 
      tttgtgtac gtaaacata aattacgtt cctcttaat 
      agtttcctt gtgttgcaa cttcgccta tttcaacca 
      atactatgc taactccga ttttagtgt ttgcctctt 
      gtctgttat gaatgctgg atactacgc ccacagcag 
      ccccagtcg agaatctgg agttgtaag tgactgtga 
      cacaatggc cgaacttgc agccttgct gcgctccga 
      aatcccaga ggggcgcag caacctgac cgacaacca 
      cacgaacct ggaaagggt tgccgaata ttgcgaagc 
      taactattt ccagtcgaa taacaagcg cgtggcttt 
      ggaagaaac caaaaacta cacaacaca gtctttggc 
      aagtgtggc ctaccagat caacacact cgctttcaa 
      ttttctgca actgttgga gttgcaagc ggcacagct 
      ggccgagat ggagagcca gatgaacca catctcgca 
      aacggtgat gatccacaa ggagaaggt tgcaaggcg 
      cgagattgg agtgttgac ggccaacaa gtcaaccag 
      ccgacagta taaaatcat cgcgcctgc taacccgga 
      gaaacccat caagtacct ccggaaacc gattgacta 
      caacatttt cgatgatat cgggcatgg ggtgcggag 
      cagtgggac ccccagaca gcaaaaaca gaggggttc 
      cagtcaggg tagcatcca gtcgctcac ggccaatcc 
      catggtggg cccagcacc caccaccaa accgccaac 
      gcctccgca agtatcaag aacatcaag taaagtttc 
      tgtttcggg aggtacgtt gagtaaagg cagtcgcga 
      atatcgtac tcctccagc ggtggcgcc tcctcaggt 
      tcctagtca ctacgcccc caattatcc cattggcca 
      tgcaagacg ttcgaccga aagggggcc cgggtacag 
      cactttgcc catggctcc aggtcaaca tcaggcgat 
      gtcgccacc acaagttgg catggtaca tcccatgag 
      tataaatca acagcaaca ccaaaacca acaacatcc 
      tcaaacacc accgccgcc gcctccgcc cggtaatgt 
      gggaaacta catccaagc ggagcacca tatgagcat 
      gccgccgcc gccttcgcc gttgatcag ccaagaaat 
      gcaaatgcc gtcgaacca gcaccaccc gatgatgat 
      gacccagca tgccccagt gccgcatgg aagcctggg 
      aatgcacac tttgtccca tgcccatgg ccaacggct 
      tgcccgaag tagcggtgc acattcgcc tccattgcc 
      tcctccacc gccgccaga agacgaaca cgaggcttt 
      tggcagacc tcgtacgag tggagtcat gcccattgt 
      gcccgatga ggaggattt gcccggatg ggtacccaa 
      gaattatat agagaaagt tgtggcgat ttacgatta 
      ttacgctga taaagatga tgaattgag tttccaaga 
      aagttcggt tatttatgt gctgaagaa aaatgatga 
      tggttggtg ggaaggtgt catggatgg aattacggg 
      attgtttcc tgggaatta cgtggaacc ttgtgtata 
      atttactaa ttaggtttc cgcacgtaa taattatgc 
      aaatcaatt attaatgtt attaagtgt aacccgact 
      ttaaagaaa attcttcaa agtgtctag aatgaaact 
      ttgtgtaga tgttttaca gttctgtag acggtatga 
      tggtaattg tataattta tattatttt tctccaaac 
      gtttgtaat acatttata agtaataaa aattctata 
      tcata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY