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Gene information
General information
TC000411 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11821059..11822959
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000411
    iBeetle screen iB_00087

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature ventral shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature dorsal shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature fluorescence not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    remarks: animals lost, low validity due to technical reasons - should be repeated: stink gland phenotype not scorable, adult phenotype not scorable, lethality not scorable,
    adult progeny in the well
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost, low validity due to technical reasons - should be repeated: stink gland phenotype not scorable, adult phenotype not scorable, lethality not scorable,
    adult progeny in the well
    Fertility
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000411 RA
      MLGFLTLLV TFVTLLVFL FYKFSKERP ENFPPGPPS 
      LPIWGSYWF LLKENYNLL HLSFETLGK RYKTDILGF 
      FLGNAPTVV TLSYELTKE MLSRDEFMG RNDTIIVQT 
      RSRGSPKGI FFTDGPVWK QQRRFALRH MRDYGFGRR 
      SENMENLAH QETNYLIDF LTKEPSQGD LDVCKKKGL 
      VLVPDFMYG SLINTVMLV LTSRHFEHQ EVRKYAKAA 
      FRFMKSEDS TGGAITITP WLRYIAPTF FGFTSAVDD 
      NDMLIDFMK GLIEEHVET FSDDHHRDF IDDYISESI 
      KQGIEIDYL QLALITLDF MFPSPVGVG HTLSFYFAH 
      LINNPEIQT KIQEEIDQV VGRSRLPSL DDRKDLPYF 
      EASIRENLR LMPVTPLGV TRRCVKDTM LGGHFIPKD 
      SFVLANLWT AHRDPRIWD DPEEFRPER FLDSDGKLL 
      RKDYTIGFG AGKRLCAGE TYARNTMFL IMSGLLQNF 
      TFKSPYNRP IDLKDVLPG VTLSLKETW ISAVPR
    CDS sequence
    • >TC000411 RA
      atgttgggc ttcctgacg ttgcttgta acattcgtc 
      actttgctg gtgtttcta ttttacaaa ttttcaaaa 
      gagaggcct gaaaatttt ccgccaggt ccgcccagt 
      ttgccaata tggggtagt tactggttt ctgttaaaa 
      gaaaattac aatttgctc cacctctcg tttgagacg 
      cttggcaag cggtacaag acggatatt ttgggtttt 
      tttctggga aatgccccg actgtggtc accttgagt 
      tacgaactc actaaagaa atgttgtcg cgggatgag 
      ttcatgggt cgaaacgac accataatc gtgcaaacg 
      aggagtcgg ggaagtccc aagggtatt tttttcact 
      gacggtccg gtctggaag cagcagagg aggtttgcg 
      ttgcgacat atgagggac tatggtttt ggccgacgt 
      tcggaaaat atggaaaat ctcgcgcat caggaaacc 
      aattatttg attgatttc ctgactaag gagccgagt 
      caaggggac ttggatgtg tgcaaaaag aaaggtctt 
      gtgctagtg cccgatttc atgtacggg tctcttatc 
      aacaccgtg atgttggtg ctaacttcg aggcacttt 
      gagcaccaa gaagtgcgg aaatatgcg aaagctgcg 
      ttcaggttt atgaaaagt gaggactca actggggga 
      gctattact ataactccc tggttgaga tacattgca 
      ccgactttc ttcgggttc acctcggcg gtcgacgac 
      aatgacatg ttaattgat tttatgaaa ggactgatt 
      gaggagcac gttgagacg ttctcggat gaccaccat 
      cgcgacttt attgacgac tacattagt gaatccatc 
      aagcaaggc attgaaatt gattatttg cagcttgct 
      ttaataacg ctggatttc atgttcccc tctcctgtc 
      ggagtcgga cacacttta agtttttat tttgcgcat 
      ctaattaac aatcctgag attcaaact aaaatccaa 
      gaggaaatt gaccaagtt gtgggccgt tcgcggcta 
      ccaagccta gatgaccga aaagatctt ccgtatttc 
      gaagcaagc attcgggaa aatctaaga ttgatgcca 
      gtcacccct ttgggggtt acaagacgt tgtgtgaaa 
      gacacaatg ttgggtggg cactttatt ccgaaagat 
      tcatttgtg ttggcaaat ctttggaca gcgcataga 
      gatcctcgc atttgggac gatccagag gagtttcga 
      ccagaaagg ttcttagac agtgatggt aaattactg 
      aggaaggac tacacgata ggatttgga gcaggtaaa 
      cgactgtgt gctggggag acgtatgca cgcaatacc 
      atgtttctg ataatgtcc gggcttttg caaaatttt 
      acgtttaaa tcgccgtac aatagaccc attgatctt 
      aaagatgta cttcctggt gtaacatta tcgttgaaa 
      gaaacgtgg atttcagca gtaccgaga taa
    mRNA sequence
    • >TC000411 RA
      acattaacc agttagtct taaagatgt tgggcttcc 
      tgacgttgc ttgtaacat tcgtcactt tgctggtgt 
      ttctatttt acaaatttt caaaagaga ggcctgaaa 
      attttccgc caggtccgc ccagtttgc caatatggg 
      gtagttact ggtttctgt taaaagaaa attacaatt 
      tgctccacc tctcgtttg agacgcttg gcaagcggt 
      acaagacgg atattttgg gtttttttc tgggaaatg 
      ccccgactg tggtcacct tgagttacg aactcacta 
      aagaaatgt tgtcgcggg atgagttca tgggtcgaa 
      acgacacca taatcgtgc aaacgagga gtcggggaa 
      gtcccaagg gtatttttt tcactgacg gtccggtct 
      ggaagcagc agaggaggt ttgcgttgc gacatatga 
      gggactatg gttttggcc gacgttcgg aaaatatgg 
      aaaatctcg cgcatcagg aaaccaatt atttgattg 
      atttcctga ctaaggagc cgagtcaag gggacttgg 
      atgtgtgca aaaagaaag gtcttgtgc tagtgcccg 
      atttcatgt acgggtctc ttatcaaca ccgtgatgt 
      tggtgctaa cttcgaggc actttgagc accaagaag 
      tgcggaaat atgcgaaag ctgcgttca ggtttatga 
      aaagtgagg actcaactg ggggagcta ttactataa 
      ctccctggt tgagataca ttgcaccga ctttcttcg 
      ggttcacct cggcggtcg acgacaatg acatgttaa 
      ttgatttta tgaaaggac tgattgagg agcacgttg 
      agacgttct cggatgacc accatcgcg actttattg 
      acgactaca ttagtgaat ccatcaagc aaggcattg 
      aaattgatt atttgcagc ttgctttaa taacgctgg 
      atttcatgt tcccctctc ctgtcggag tcggacaca 
      ctttaagtt tttattttg cgcatctaa ttaacaatc 
      ctgagattc aaactaaaa tccaagagg aaattgacc 
      aagttgtgg gccgttcgc ggctaccaa gcctagatg 
      accgaaaag atcttccgt atttcgaag caagcattc 
      gggaaaatc taagattga tgccagtca cccctttgg 
      gggttacaa gacgttgtg tgaaagaca caatgttgg 
      gtgggcact ttattccga aagattcat ttgtgttgg 
      caaatcttt ggacagcgc atagagatc ctcgcattt 
      gggacgatc cagaggagt ttcgaccag aaaggttct 
      tagacagtg atggtaaat tactgagga aggactaca 
      cgataggat ttggagcag gtaaacgac tgtgtgctg 
      gggagacgt atgcacgca ataccatgt ttctgataa 
      tgtccgggc ttttgcaaa attttacgt ttaaatcgc 
      cgtacaata gacccattg atcttaaag atgtacttc 
      ctggtgtaa cattatcgt tgaaagaaa cgtggattt 
      cagcagtac cgagataag aacgaaaat aattgtaac 
      aaaactgcc aataaaaga ttgttttta tttatgtgt 
      gatgactct atttaaaat atataagat aattattgt 
      actaaatgg ataaagtgg taataaaaa aatgtgtcc 
      cgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY