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Gene information
General information
TC000402 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11910932..11913009
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000402
    iBeetle screen iB_00085

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    pupation delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000402 RA
      MLCGPDFLS SSDEDGFSD DNDYYDFKE RLKASQRQA 
      NLKVETEAE REKQHLKSV FSAIAYEKE DVFLAELEK 
      GLDPNVDLD QGWTPLVLA CTVANEKFI SALVEKNVN 
      VNQPRDGLT PLMLLCNIN SKDDVTEAK ILSCVKLLL 
      NKGADVNMI DNKRRTAIM FAAYNGHLE TVKLLLPLV 
      DKEVEDNQR WNVLFWAVE GSNVKIVEY LLKEGFNCD 
      KTDIRGNTV AELAHSRGF QDILDLFPK DPFDEFYQY 
      LNNNELNFE QTFNNLAPS EKPQFFTDI CEILYGTRS 
      ESVMPLLRD RNTSLFEFL TLNDSKLKE IGVRFPFQR 
      NRILGGLYK FHKHQFQPK SIPFVMKNQ LYTNIDVAA 
      AVLTCVKQT IAMEASLKF ILNNCETSL EPKEIRVLL 
      QRNRQQIRR LRQVTDALV TKTEQVGFL FVYFILLVY 
      FSGIRA
    CDS sequence
    • >TC000402 RA
      atgttgtgc gggccggac tttttaagt agcagtgat 
      gaagacggg ttttcggac gataacgat tattacgac 
      tttaaggaa agactgaaa gctagccaa agacaagca 
      aacctgaaa gtcgagact gaagctgaa agagaaaaa 
      cagcacctg aaatcggtc ttttcagca atcgcttat 
      gaaaaagag gatgtcttc cttgcagag ctcgaaaaa 
      gggttagac ccaaacgtt gatttagac caaggatgg 
      actcctctc gttcttgcc tgtaccgtc gcaaacgaa 
      aaatttatt tccgctctt gtggaaaaa aatgtcaat 
      gttaatcaa ccccgagat ggtttaact ccgttaatg 
      ttattgtgc aatataaat tcgaaagac gacgttact 
      gaggcaaag attttatct tgtgttaag ctgcttcta 
      aataaggga gcagatgtt aatatgatc gataataaa 
      cgaagaact gccattatg ttcgcggct tataacggc 
      catttagag actgttaaa ttattattg cctttagtg 
      gacaaagag gttgaagat aatcagcgt tggaatgtg 
      ctattctgg gctgttgaa ggcagcaat gttaaaata 
      gtcgaatat ctcctaaag gaaggtttc aactgtgat 
      aaaactgac atcaggggg aatacagtt gcggaatta 
      gcccacagt aggggattt caagacatt ctagatcta 
      ttccccaaa gacccattc gacgaattt taccaatac 
      ttgaataac aatgaactc aatttcgag caaactttc 
      aataatctc gcccctagc gaaaagccg cagtttttc 
      accgatatt tgtgaaatt ttatacgga acgcgtagc 
      gaaagcgta atgccactt ctgagggac agaaacacc 
      tccctgttc gagttttta acactcaat gattccaaa 
      ctgaaagaa atcggtgtt cgattcccg tttcagcga 
      aaccgcatt ttaggggga ctttacaag tttcacaaa 
      caccagttt cagccaaaa tctataccg tttgtgatg 
      aaaaaccaa ttatacact aatattgat gtcgcagct 
      gctgttttg acgtgtgtt aaacaaaca atagcgatg 
      gaagccagc cttaagttt attttaaat aattgtgaa 
      acaagtctc gagccaaaa gagattcgc gttttgcta 
      cagaggaac agacaacag attcgaaga ctgagacaa 
      gtgacggac gcacttgtg actaagact gaacaggtg 
      ggtttttta tttgtttac ttcatttta ttggtttat 
      ttcagtggg attcgagct taa
    mRNA sequence
    • >TC000402 RA
      cgcgcacct gtttacgat tttttataa cctaaaaaa 
      acattctac agtaaaagc ctaatgaaa cacgtaaaa 
      tgttgtgcg ggccggact ttttaagta gcagtgatg 
      aagacgggt tttcggacg ataacgatt attacgact 
      ttaaggaaa gactgaaag ctagccaaa gacaagcaa 
      acctgaaag tcgagactg aagctgaaa gagaaaaac 
      agcacctga aatcggtct tttcagcaa tcgcttatg 
      aaaaagagg atgtcttcc ttgcagagc tcgaaaaag 
      ggttagacc caaacgttg atttagacc aaggatgga 
      ctcctctcg ttcttgcct gtaccgtcg caaacgaaa 
      aatttattt ccgctcttg tggaaaaaa atgtcaatg 
      ttaatcaac cccgagatg gtttaactc cgttaatgt 
      tattgtgca atataaatt cgaaagacg acgttactg 
      aggcaaaga ttttatctt gtgttaagc tgcttctaa 
      ataagggag cagatgtta atatgatcg ataataaac 
      gaagaactg ccattatgt tcgcggctt ataacggcc 
      atttagaga ctgttaaat tattattgc ctttagtgg 
      acaaagagg ttgaagata atcagcgtt ggaatgtgc 
      tattctggg ctgttgaag gcagcaatg ttaaaatag 
      tcgaatatc tcctaaagg aaggtttca actgtgata 
      aaactgaca tcaggggga atacagttg cggaattag 
      cccacagta ggggatttc aagacattc tagatctat 
      tccccaaag acccattcg acgaatttt accaatact 
      tgaataaca atgaactca atttcgagc aaactttca 
      ataatctcg cccctagcg aaaagccgc agtttttca 
      ccgatattt gtgaaattt tatacggaa cgcgtagcg 
      aaagcgtaa tgccacttc tgagggaca gaaacacct 
      ccctgttcg agtttttaa cactcaatg attccaaac 
      tgaaagaaa tcggtgttc gattcccgt ttcagcgaa 
      accgcattt tagggggac tttacaagt ttcacaaac 
      accagtttc agccaaaat ctataccgt ttgtgatga 
      aaaaccaat tatacacta atattgatg tcgcagctg 
      ctgttttga cgtgtgtta aacaaacaa tagcgatgg 
      aagccagcc ttaagttta ttttaaata attgtgaaa 
      caagtctcg agccaaaag agattcgcg ttttgctac 
      agaggaaca gacaacaga ttcgaagac tgagacaag 
      tgacggacg cacttgtga ctaagactg aacaggtgg 
      gttttttat ttgtttact tcattttat tggtttatt 
      tcagtggga ttcgagctt aaaacctgc tgataggat 
      tacgaaaaa gcggcgcaa cgttccgtg ggccacctt 
      ttggctcac tttcgggac aatctctct acttgtatt 
      tttgaaagt ga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY