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Gene information
General information
TC000388 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12185398..12188045
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000388
    iBeetle screen iB_00084

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000388 RA
      MLTTNECLN LDDYVFKSS PESCSGNSC FTDCTDLPS 
      DEDFLQQLS ADLDIPLLL NPGEDELSL LNSFFDKTP 
      EEIMSEIAL PSSPEISDE AKWDPGSLC SEVKIEPDS 
      FSSSSSNKS NSPVQSPDV RFFEIKDEL KNEPFSPPG 
      EFDSNIVIQ TVQNPLKLS PNSKVVPIQ PKLPVRSKV 
      VVLKNTDLK RSAPTVSPP PPKVVVLEN IATVPITTA 
      QLTPTTLSN SIINNCTVP PVILKGSKN LAVANIDPK 
      IIKRHQRKI KNRESACLS RKKKKDYLT SLENQVKDL 
      TTENQQLKL ENQQLKERL KKYESNPSQ SSNIKTGIV 
      LCMFLFSVA FNLDYVRNP FFAKNQANR IKDNSPKVL 
      DHHGRNLLW APDVEEKNN STFSPFSMC PASVNQTES 
      ARLALELER WIGKPKESV PLNTTRLSV ANKSRSLRR 
      KKAKKYETS FISSMYDTQ SVAKKLSVE PNNELQVFS 
      PTPEQLYSE FFEAINRQD DTFYVVSFS EHHMLLPAL 
      HHNKTRRPK MSLIMPAVL PSGNTTQPS LVPLMQIDC 
      EVLDTRLIQ IRYGSIPQH LRKHGNATY QGNDSVTKP 
      SETAANYTA RKAYRPYFI KHKAVRASG QDFYN
    CDS sequence
    • >TC000388 RA
      atgttgaca acaaatgag tgcttaaac ttagacgat 
      tatgtgttt aaaagctcc cccgagtcg tgcagtggc 
      aactcctgt ttcacagac tgcaccgat cttccgtca 
      gatgaagat tttctccag caactatca gcagactta 
      gacatccca ctgttgcta aatcccggc gaagatgaa 
      ttgagttta ctaaattct ttctttgac aagactcct 
      gaggagatc atgtcagaa atcgccctt ccttcgtca 
      cccgaaatc agcgacgaa gccaaatgg gaccccggg 
      tccctttgc tcagaagtt aagatcgaa cctgacagt 
      ttcagttcc tcaagttca aataaatca aattcgccg 
      gttcagtca cccgatgtg agatttttc gaaattaaa 
      gacgagcta aaaaacgag ccgttttcg ccccctggc 
      gagttcgac tcaaacatc gtcattcaa acagtacaa 
      aaccctctg aaattatca cccaatagc aaagtagtc 
      ccgatccag cccaaatta cccgtaaga agcaaagta 
      gtggttttg aaaaacacc gatctcaaa agaagtgcc 
      cccactgtg agcccgccg ccgccaaaa gtcgtggtt 
      ttggaaaac atcgccact gtccccata accaccgcg 
      caattaacg ccaacaact ttaagcaac tctattata 
      aataattgc acagttccg cctgttata ctgaaagga 
      agtaaaaat ttagcggtg gcaaacatt gacccgaaa 
      ataataaag cgccaccaa cgcaaaatc aaaaatcgc 
      gagtcggcg tgcttatcg cgaaaaaag aaaaaagac 
      tacttgaca tcattggaa aaccaagtt aaagacttg 
      accacggaa aaccaacaa ctcaaattg gaaaatcaa 
      caattgaaa gagcgcttg aaaaaatac gaaagcaat 
      ccgtcacaa tcaagtaac ataaaaaca ggcattgtc 
      ttgtgcatg ttcctgttt tctgtggcc tttaatttg 
      gattacgtc agaaatccg ttttttgcc aaaaaccag 
      gccaatcgg attaaggac aattcaccc aaggtttta 
      gaccatcat gggagaaac ctactgtgg gctcctgac 
      gtagaagag aagaacaac tcaaccttt tcacccttc 
      tccatgtgt cctgcctca gtcaaccag accgagagt 
      gcgagacta gccttagag ttagaaagg tggattgga 
      aagccgaaa gaaagcgtt cctttaaac acgacgcgc 
      ttgagtgtc gctaataag tcgcgcagt ttgcgccga 
      aagaaggcc aaaaaatac gagacctcg ttcatttcg 
      tccatgtac gacacccaa agcgtcgcc aaaaagctc 
      tcagtcgag ccaaataac gagctccag gtgttttcg 
      ccgactcct gagcagctt tatagtgaa tttttcgaa 
      gcaataaat cggcaagac gacactttt tatgtcgtc 
      tcattcagc gaacatcac atgctattg ccggctctt 
      caccacaat aagaccaga aggccgaag atgtcgctt 
      ataatgccc gcagtgttg cccagtggt aacacgact 
      caacccagt ttggttcct ttaatgcaa atcgattgc 
      gaggttttg gatacgaga ttaatacaa attaggtac 
      gggtctatc ccgcaacat ttacgaaaa cacggaaat 
      gcgacatat cagggtaat gacagtgtg accaaaccc 
      agtgaaact gcggcgaat tacacggca aggaaagcc 
      tacaggccg tattttatc aaacataag gccgtaagg 
      gccagtggg caagatttt tataattga
    mRNA sequence
    • >TC000388 RA
      acgtcagca attaacaaa tgtgcggtt atgtgtcac 
      tgttcggca tttaataaa tacatgtag ttatttatt 
      gtagaagaa atagttatg ttgacaaca aatgagtgc 
      ttaaactta gacgattat gtgtttaaa agctccccc 
      gagtcgtgc agtggcaac tcctgtttc acagactgc 
      accgatctt ccgtcagat gaagatttt ctccagcaa 
      ctatcagca gacttagac atcccactg ttgctaaat 
      cccggcgaa gatgaattg agtttacta aattctttc 
      tttgacaag actcctgag gagatcatg tcagaaatc 
      gcccttcct tcgtcaccc gaaatcagc gacgaagcc 
      aaatgggac cccgggtcc ctttgctca gaagttaag 
      atcgaacct gacagtttc agttcctca agttcaaat 
      aaatcaaat tcgccggtt cagtcaccc gatgtgaga 
      tttttcgaa attaaagac gagctaaaa aacgagccg 
      ttttcgccc cctggcgag ttcgactca aacatcgtc 
      attcaaaca gtacaaaac cctctgaaa ttatcaccc 
      aatagcaaa gtagtcccg atccagccc aaattaccc 
      gtaagaagc aaagtagtg gttttgaaa aacaccgat 
      ctcaaaaga agtgccccc actgtgagc ccgccgccg 
      ccaaaagtc gtggttttg gaaaacatc gccactgtc 
      cccataacc accgcgcaa ttaacgcca acaacttta 
      agcaactct attataaat aattgcaca gttccgcct 
      gttatactg aaaggaagt aaaaattta gcggtggca 
      aacattgac ccgaaaata ataaagcgc caccaacgc 
      aaaatcaaa aatcgcgag tcggcgtgc ttatcgcga 
      aaaaagaaa aaagactac ttgacatca ttggaaaac 
      caagttaaa gacttgacc acggaaaac caacaactc 
      aaattggaa aatcaacaa ttgaaagag cgcttgaaa 
      aaatacgaa agcaatccg tcacaatca agtaacata 
      aaaacaggc attgtcttg tgcatgttc ctgttttct 
      gtggccttt aatttggat tacgtcaga aatccgttt 
      tttgccaaa aaccaggcc aatcggatt aaggacaat 
      tcacccaag gttttagac catcatggg agaaaccta 
      ctgtgggct cctgacgta gaagagaag aacaactca 
      accttttca cccttctcc atgtgtcct gcctcagtc 
      aaccagacc gagagtgcg agactagcc ttagagtta 
      gaaaggtgg attggaaag ccgaaagaa agcgttcct 
      ttaaacacg acgcgcttg agtgtcgct aataagtcg 
      cgcagtttg cgccgaaag aaggccaaa aaatacgag 
      acctcgttc atttcgtcc atgtacgac acccaaagc 
      gtcgccaaa aagctctca gtcgagcca aataacgag 
      ctccaggtg ttttcgccg actcctgag cagctttat 
      agtgaattt ttcgaagca ataaatcgg caagacgac 
      actttttat gtcgtctca ttcagcgaa catcacatg 
      ctattgccg gctcttcac cacaataag accagaagg 
      ccgaagatg tcgcttata atgcccgca gtgttgccc 
      agtggtaac acgactcaa cccagtttg gttccttta 
      atgcaaatc gattgcgag gttttggat acgagatta 
      atacaaatt aggtacggg tctatcccg caacattta 
      cgaaaacac ggaaatgcg acatatcag ggtaatgac 
      agtgtgacc aaacccagt gaaactgcg gcgaattac 
      acggcaagg aaagcctac aggccgtat tttatcaaa 
      cataaggcc gtaagggcc agtgggcaa gatttttat 
      aattgagtt tcagcaaac caaaaattt taattaggg 
      tttttcagt gaaattgtg atatgattt gtattgtgg 
      tctcaaata aaatttttc aggaagtgt gtagatagt 
      ttgagctct acttatcaa tgcaaataa tgtaaggtg 
      atgggattt aacgcgttt tgtgtacat attgctttt 
      atttagatg tgattatgt ttgtgtgga ataaccata 
      acagagtac ctaagtttt tgtaaattt tttattgtg 
      tatattgca ctttttttt actagaatg tgtaaatcg 
      ttgataaat tgagtttcc tcttgtagc acttatttt 
      accatatgc cactgaatg tacaaatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY