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Gene information
General information
TC000382 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 6723695..6732124
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000382
    iBeetle screen iB_00083
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4

    vitellarium size decreased - Number of animals showing the phenotype: 4
    tropharium structure irregular - Number of animals showing the phenotype: 4
    tropharium potentially degenerated - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection



    Pupal morphology
    head potentially anteroposterior-axis shortened - Number of animals showing the phenotype: 5

    antenna orientation irregular - Number of animals showing the phenotype: 7

    wings shortened - Number of animals showing the phenotype: 8

    Adult morphology
    wings not closed - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000382 RA
      MSFFNNIKK VLHFGGNDA KKKKVFNNV RMECDPEEF 
      WDMIGELGD GAYGKVYKA QHKHTGQLA AAKMCRLDG 
      EDDLSDFMI EIDILTECK HPNIVELHE AFQKEQQLW 
      MLIEYCDGG ALDSIMTEL EKPLTELQI AYVCQNMCK 
      GLEFLHKNH VIHRDLKAG NVLLTMEGG VKLADFGVS 
      AKNKSTMQK HDTFIGTPY WMAPEVVLC ETFRDNPYD 
      FKVDVWSMG ITLIEFAQM EPPNHEMSP MRVLLKIQK 
      SDPPKLEQP SKWSKEFND FVSNCLVKD PQKRPTCDE 
      LLKHPFINC TLDSKPIRD LLLEYKAEV VEEELTDDD 
      PEDNRTSHV PLDIEDDST SVKSSEVDN KVNDLESPV 
      INVEEETAK DLTASTPSN VQADKKPLE KKDEVQRKT 
      SRDKGKAPP PPIQPQASS STEKPPAPA APVAIPNLP 
      TPPSSPITP PATPPSSSP VHPGSEPAK PEENKDNVD 
      NIKEVLEKQ LKKRQSEGD VASEGVTVS ATSDDTVSN 
      FVVVSSQSE RNKLNTSTI TINSDLPDP SNSLASNIS 
      QVTVVTTHP PVIIDNTQC VSRSSTSPS NSTNEVVIV 
      ANETNKTHV ESSDDECYP SLDSLEYPP PSEFRDRPL 
      KAKKLDESE VLIVDSSYV INDSGLDVS DDNSRLLDT 
      SHVSVVKID EEKVQVKDS TSYLDKSGD NFRSSTSDL 
      SNASSCKTG SSDEVRGTS ITLDAPPNE IKAKMNGQV 
      VLNGKGFDN YESALSDRS HSDCGSVRS NDSHRRPVS 
      AKSIEHSDI ESISIASHE SRGSTGKEP KNDRLSELD 
      ENDDGVILR KKAQKTKRT SLAIANRKT RKRTRKFII 
      DGVVITTTT SKVICPDED NYNLADLHN DRKQELREL 
      KYLQKQEQK QFQDLAFKE NLTLEQQDK KFEQERVNL 
      ERTYENDLE MLSRQQRMQ IEKAEAHQD AELRAMSKK 
      IRGEQEREL KIFRDGLKQ ELRLLKAEI DLLPKERRK 
      AEFKLRKEK MDADHLERE KAFLEKLNE NHESSLRRL 
      SDSHREKIA LMERQFLQQ KQQLTRTRE AALWEIEER 
      QIHEKHQLM KRQIKEIFI LQRHQMLTR HEKEKEQIK 
      RRAARKEEE LLKKQAAEK RSLPKRIRT EMKARELMF 
      RESMRISIS GTNDPEAEK NKFKEFQEK EKKRYQAEQ 
      QRFELKHQR QLEELRAMS DATIKELEQ LQNEKRKML 
      LEHETMKLK QREEAFSRE LKEWKAQLK PRKQKLEEA 
      FAQQLEEQE RVYGPISTP LALPPDLPD LSQESCKIG 
      MGSTRSSLS SVSEG
    CDS sequence
    • >TC000382 RA
      atgtctttc ttcaacaac attaaaaaa gtgcttcat 
      ttcggcggc aacgatgcg aaaaagaaa aaagtgttc 
      aacaacgtg cgcatggag tgcgacccc gaggagttt 
      tgggacatg atcggggag ctgggcgat ggggcttac 
      ggcaaggtc tacaaagcc cagcataaa catacggga 
      cagctggcc gcggcgaaa atgtgccga ctggatggg 
      gaggacgac ctctccgac ttcatgata gaaattgat 
      attttaacc gagtgcaaa catccaaat attgtcgag 
      ttgcacgaa gccttccag aaagagcag caactctgg 
      atgttaatc gaatactgt gatggtggg gccttagat 
      agtattatg actgaatta gaaaaacct ctaactgaa 
      ttacaaatt gcttatgtg tgccaaaac atgtgcaag 
      ggactcgag ttcttgcac aaaaaccac gttatacac 
      agggacttg aaagcggga aatgtgctg ctgaccatg 
      gaaggaggg gtcaagctg gccgacttc ggagtctcc 
      gctaaaaat aaaagcacg atgcaaaaa cacgacacg 
      ttcatcgga acgccatac tggatggcc cccgaagtg 
      gtcctttgt gagactttc cgcgacaat ccgtacgat 
      ttcaaagtg gacgtgtgg tcgatgggc ataactctc 
      attgaattc gcccaaatg gagccaccc aaccacgaa 
      atgtcaccc atgagggtg ttactcaaa atccaaaaa 
      tccgatccg cccaaattg gaacaaccg tcgaagtgg 
      tcgaaggag tttaacgat ttcgtgtca aattgtctg 
      gtcaaggat ccgcaaaaa agaccaacc tgtgacgag 
      ttgttgaag catccgttt attaattgt acgctggac 
      tcgaaaccg atcagggat ttgttgttg gagtacaag 
      gcggaggtt gttgaagag gaattgaca gatgatgat 
      cccgaggac aaccgcact tctcatgtt cctctggat 
      atagaagat gactccact tctgtaaag agcagcgaa 
      gttgacaat aaagtaaat gacctagaa tctcccgta 
      attaatgta gaagaagaa actgctaaa gatttgaca 
      gcttcgacg cctagcaat gtccaagcc gacaaaaaa 
      ccgctggag aagaaagac gaagtccag aggaaaacc 
      tcccgggat aaaggtaaa gcgccccca cctcccatc 
      caaccccaa gcgagttcg tcgactgag aaacctcca 
      gccccggct gcacctgtc gccattccc aacctacca 
      acgccccca tcttcgccc atcacacct ccagcaact 
      ccgccaagt tctagtcca gttcatcca ggtagcgaa 
      cccgcaaaa cccgaagaa aacaaagac aacgtcgat 
      aatattaaa gaagtatta gaaaaacaa ttgaagaag 
      cggcagtct gaaggcgac gtagcgagt gagggagtc 
      acagtcagt gcgacatct gacgacact gttagtaac 
      tttgttgtg gtttcgtca caatctgaa cgaaacaaa 
      ctaaacact tccactatc accattaac tctgatctt 
      cccgatccc tcaaacagt ttagcgtcg aatatcagt 
      caggtgact gtagtaacg actcatccg cccgttatt 
      atcgacaat actcaatgc gtgtcgcga tcttcaact 
      tcccccagt aatagtaca aacgaggtc gtgatagtt 
      gcgaacgaa acgaataaa acacacgtt gaatcatca 
      gacgatgaa tgttacccg agtttggat tcgttggaa 
      tacccccca cccagcgag ttccgcgac aggccgctc 
      aaagcgaaa aaattagac gaaagcgag gttttaatc 
      gtcgattcg agttatgtt ataaacgat tcgggtctg 
      gacgtatcc gacgataac tcccgactt ttggatact 
      agtcacgtg tctgttgta aaaatcgac gaagagaaa 
      gtccaagtc aaggattcg acgtcatac ctcgacaag 
      tcgggtgat aatttccga agttcgacg agtgattta 
      tcgaacgct tcctcgtgc aaaacggga agtagcgat 
      gaagttcgg ggcacttcg ataacactc gacgcgccg 
      ccgaacgaa attaaggca aaaatgaac ggacaagtt 
      gtgctaaac ggcaaaggg ttcgacaat tacgagtcg 
      gctttgtcc gaccgttca cacagcgac tgtggatcg 
      gttcgcagc aacgactcg caccgaaga ccggtttcg 
      gccaaaagt atcgagcat tcggacatt gaaagtatc 
      tcaatagct agtcacgag agtcgggga agcaccggc 
      aaagaaccg aagaacgac cgactgtcg gaattggac 
      gagaacgac gacggtgtc atcttgcgc aagaaagcc 
      cagaaaaca aaaagaacg tctctagca atcgccaat 
      cgaaagacc cgcaaaaga acccggaaa tttataata 
      gatggcgtt gtcattacc acgactacc agcaaagtg 
      atttgtccc gacgaagat aattacaac ttggccgat 
      ttgcataat gacaggaaa caagaattg cgcgaattg 
      aaatacttg caaaaacag gaacaaaaa caattccag 
      gatttggcg tttaaggag aatttaacg ctggaacag 
      caggataaa aagttcgaa caagaacga gtgaatctc 
      gaacggacg tacgaaaac gacttggag atgttgagt 
      cggcagcag aggatgcaa atcgagaaa gccgaagcg 
      catcaagat gccgaactc cgagctatg tcgaagaaa 
      attcgaggg gagcaagaa cgcgagttg aagattttc 
      cgagacggg cttaagcaa gaattgaga ttacttaaa 
      gccgaaatc gatttgtta ccgaaggag cggcgcaag 
      gccgaattt aaactgcga aaggagaaa atggatgct 
      gatcatctc gagcgcgag aaggcgttt ttggagaaa 
      ctcaatgag aatcatgag agttcgttg cgaaggctg 
      agcgattcg catcgtgag aaaattgcg ttgatggaa 
      aggcagttc ttgcagcag aagcagcag ttgacgagg 
      acgcgcgaa gcggcgctt tgggagatc gaagagagg 
      cagattcat gagaagcat cagttgatg aagagacag 
      attaaggag atatttatt ctgcaaagg catcagatg 
      ttgacgagg catgagaag gagaaggag cagattaag 
      agacgggcg gcgaggaag gaagaggag ttgttaaag 
      aaacaagcg gcggaaaaa aggtcgctg ccgaagagg 
      atcaggacg gagatgaag gcgagagag ctgatgttc 
      agggagtcg atgaggata tcaatttcc gggacgaac 
      gatccggaa gcagagaag aataagttt aaagagttc 
      caagagaag gagaaaaag cgctaccag gccgagcag 
      caacgcttc gagctgaaa caccagcgc caactcgaa 
      gagctccgc gcaatgagc gacgccacc atcaaagag 
      ctcgaacaa ctccagaac gaaaagcgt aaaatgctc 
      ctcgaacac gaaacgatg aaactaaag caacgcgaa 
      gaagcattc agtcgcgaa ctaaaagaa tggaaagct 
      caactcaaa ccgcgaaaa cagaaattg gaagaggcg 
      tttgcccag cagttggaa gaacaggaa agggtttat 
      gggccgatc agtactcct ctggctttg cctccagat 
      ttgcccgat ctgagccag gagagttgc aagatcggg 
      atggggtcg actaggagc agtttatcg tcggtttcg 
      gagggatag
    mRNA sequence
    • >TC000382 RA
      tagggaaaa aatggtatg gctgacggt ttagtgaaa 
      aatgaagat acacaaatg tgataaatc tgtggtgaa 
      tttctgcag tgatgttat gaaaatttc ggtggtttt 
      tgccttttt aaagctggt ttagattag caaagcaaa 
      taaacaaaa atgaggaga agaaaatcg ctttcggac 
      acctttgca gcgccttga gtgcagtgt ttgtgcgta 
      atccgtgaa cattaaaag ccaaaatgt ctttcttca 
      acaacatta aaaaagtgc ttcatttcg gcggcaacg 
      atgcgaaaa agaaaaaag tgttcaaca acgtgcgca 
      tggagtgcg accccgagg agttttggg acatgatcg 
      gggagctgg gcgatgggg cttacggca aggtctaca 
      aagcccagc ataaacata cgggacagc tggccgcgg 
      cgaaaatgt gccgactgg atggggagg acgacctct 
      ccgacttca tgatagaaa ttgatattt taaccgagt 
      gcaaacatc caaatattg tcgagttgc acgaagcct 
      tccagaaag agcagcaac tctggatgt taatcgaat 
      actgtgatg gtggggcct tagatagta ttatgactg 
      aattagaaa aacctctaa ctgaattac aaattgctt 
      atgtgtgcc aaaacatgt gcaagggac tcgagttct 
      tgcacaaaa accacgtta tacacaggg acttgaaag 
      cgggaaatg tgctgctga ccatggaag gaggggtca 
      agctggccg acttcggag tctccgcta aaaataaaa 
      gcacgatgc aaaaacacg acacgttca tcggaacgc 
      catactgga tggcccccg aagtggtcc tttgtgaga 
      ctttccgcg acaatccgt acgatttca aagtggacg 
      tgtggtcga tgggcataa ctctcattg aattcgccc 
      aaatggagc cacccaacc acgaaatgt cacccatga 
      gggtgttac tcaaaatcc aaaaatccg atccgccca 
      aattggaac aaccgtcga agtggtcga aggagttta 
      acgatttcg tgtcaaatt gtctggtca aggatccgc 
      aaaaaagac caacctgtg acgagttgt tgaagcatc 
      cgtttatta attgtacgc tggactcga aaccgatca 
      gggatttgt tgttggagt acaaggcgg aggttgttg 
      aagaggaat tgacagatg atgatcccg aggacaacc 
      gcacttctc atgttcctc tggatatag aagatgact 
      ccacttctg taaagagca gcgaagttg acaataaag 
      taaatgacc tagaatctc ccgtaatta atgtagaag 
      aagaaactg ctaaagatt tgacagctt cgacgccta 
      gcaatgtcc aagccgaca aaaaaccgc tggagaaga 
      aagacgaag tccagagga aaacctccc gggataaag 
      gtaaagcgc ccccacctc ccatccaac cccaagcga 
      gttcgtcga ctgagaaac ctccagccc cggctgcac 
      ctgtcgcca ttcccaacc taccaacgc ccccatctt 
      cgcccatca cacctccag caactccgc caagttcta 
      gtccagttc atccaggta gcgaacccg caaaacccg 
      aagaaaaca aagacaacg tcgataata ttaaagaag 
      tattagaaa aacaattga agaagcggc agtctgaag 
      gcgacgtag cgagtgagg gagtcacag tcagtgcga 
      catctgacg acactgtta gtaactttg ttgtggttt 
      cgtcacaat ctgaacgaa acaaactaa acacttcca 
      ctatcacca ttaactctg atcttcccg atccctcaa 
      acagtttag cgtcgaata tcagtcagg tgactgtag 
      taacgactc atccgcccg ttattatcg acaatactc 
      aatgcgtgt cgcgatctt caacttccc ccagtaata 
      gtacaaacg aggtcgtga tagttgcga acgaaacga 
      ataaaacac acgttgaat catcagacg atgaatgtt 
      acccgagtt tggattcgt tggaatacc ccccaccca 
      gcgagttcc gcgacaggc cgctcaaag cgaaaaaat 
      tagacgaaa gcgaggttt taatcgtcg attcgagtt 
      atgttataa acgattcgg gtctggacg tatccgacg 
      ataactccc gacttttgg atactagtc acgtgtctg 
      ttgtaaaaa tcgacgaag agaaagtcc aagtcaagg 
      attcgacgt catacctcg acaagtcgg gtgataatt 
      tccgaagtt cgacgagtg atttatcga acgcttcct 
      cgtgcaaaa cgggaagta gcgatgaag ttcggggca 
      cttcgataa cactcgacg cgccgccga acgaaatta 
      aggcaaaaa tgaacggac aagttgtgc taaacggca 
      aagggttcg acaattacg agtcggctt tgtccgacc 
      gttcacaca gcgactgtg gatcggttc gcagcaacg 
      actcgcacc gaagaccgg tttcggcca aaagtatcg 
      agcattcgg acattgaaa gtatctcaa tagctagtc 
      acgagagtc ggggaagca ccggcaaag aaccgaaga 
      acgaccgac tgtcggaat tggacgaga acgacgacg 
      gtgtcatct tgcgcaaga aagcccaga aaacaaaaa 
      gaacgtctc tagcaatcg ccaatcgaa agacccgca 
      aaagaaccc ggaaattta taatagatg gcgttgtca 
      ttaccacga ctaccagca aagtgattt gtcccgacg 
      aagataatt acaacttgg ccgatttgc ataatgaca 
      ggaaacaag aattgcgcg aattgaaat acttgcaaa 
      aacaggaac aaaaacaat tccaggatt tggcgttta 
      aggagaatt taacgctgg aacagcagg ataaaaagt 
      tcgaacaag aacgagtga atctcgaac ggacgtacg 
      aaaacgact tggagatgt tgagtcggc agcagagga 
      tgcaaatcg agaaagccg aagcgcatc aagatgccg 
      aactccgag ctatgtcga agaaaattc gaggggagc 
      aagaacgcg agttgaaga ttttccgag acgggctta 
      agcaagaat tgagattac ttaaagccg aaatcgatt 
      tgttaccga aggagcggc gcaaggccg aatttaaac 
      tgcgaaagg agaaaatgg atgctgatc atctcgagc 
      gcgagaagg cgtttttgg agaaactca atgagaatc 
      atgagagtt cgttgcgaa ggctgagcg attcgcatc 
      gtgagaaaa ttgcgttga tggaaaggc agttcttgc 
      agcagaagc agcagttga cgaggacgc gcgaagcgg 
      cgctttggg agatcgaag agaggcaga ttcatgaga 
      agcatcagt tgatgaaga gacagatta aggagatat 
      ttattctgc aaaggcatc agatgttga cgaggcatg 
      agaaggaga aggagcaga ttaagagac gggcggcga 
      ggaaggaag aggagttgt taaagaaac aagcggcgg 
      aaaaaaggt cgctgccga agaggatca ggacggaga 
      tgaaggcga gagagctga tgttcaggg agtcgatga 
      ggatatcaa tttccggga cgaacgatc cggaagcag 
      agaagaata agtttaaag agttccaag agaaggaga 
      aaaagcgct accaggccg agcagcaac gcttcgagc 
      tgaaacacc agcgccaac tcgaagagc tccgcgcaa 
      tgagcgacg ccaccatca aagagctcg aacaactcc 
      agaacgaaa agcgtaaaa tgctcctcg aacacgaaa 
      cgatgaaac taaagcaac gcgaagaag cattcagtc 
      gcgaactaa aagaatgga aagctcaac tcaaaccgc 
      gaaaacaga aattggaag aggcgtttg cccagcagt 
      tggaagaac aggaaaggg tttatgggc cgatcagta 
      ctcctctgg ctttgcctc cagatttgc ccgatctga 
      gccaggaga gttgcaaga tcgggatgg ggtcgacta 
      ggagcagtt tatcgtcgg tttcggagg gatagaacg 
      ctttaggaa acagtatac tccaaaatg tgcctctgc 
      agatccaga gcgagcatt gattaccct aactcgtgt 
      atatactta ttacacaga ctgaataat taagaaaat 
      cgtatgaaa cattttttt cgttttgtt tcttttact 
      agagagatt gaggtgacc tgataagct acttaaatt 
      tggaaattt cgaaaggga gaatttttt tcaggttgt 
      ttactgaaa acaaataaa ctattgtta gcttgcttg 
      ctaaaacta gactagttt tacgtcgat cctttttat 
      taaattaag attagaggt gattgagga tttaaaatt 
      gtgtgcatt tattaaaac cagactgac aggttacac 
      ttgttattt tgtgaaata attaagtta gattgtact 
      aatagttca aaagattgt tatatcatg ttcgtaagt 
      aagttttct ttgtattga ggtattatc taacaataa 
      atactgatt aattattat ttaacataa tagattgta 
      ttgggattg ttttttgtt tacgcttgc actactcca 
      gtctgatgt ataaatact attatagtc catgttatt 
      taaatactg aaattttta tcagctgta ttctaaggt 
      gtacataca aattccatc taacttaag tttattagg 
      acagtgtga taagatttt tatcgtctt ttagttctt 
      tactaacgt ttgccacta tcatgctgt tctgtatta 
      ttatgatgt acagtcagt gtattttat ttttatgtg 
      catattatt taccagtaa ctattatat tcaaataaa 
      taatataaa tagaa