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Gene information
General information
TC000382 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12230238..12238667
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000382
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00083

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4

    vitellarium size decreased - Number of animals showing the phenotype: 4
    tropharium structure irregular - Number of animals showing the phenotype: 4
    tropharium potentially degenerated - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    Pupal morphology
    head potentially anteroposterior-axis shortened - Number of animals showing the phenotype: 5

    antenna orientation irregular - Number of animals showing the phenotype: 7

    wings shortened - Number of animals showing the phenotype: 8

    Adult morphology
    wings not closed - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000382 RA
      MSFFNNIKK VLHFGGNDA KKKKVFNNV RMECDPEEF 
WDMIGELGD GAYGKVYKA QHKHTGQLA AAKMCRLDG 
EDDLSDFMI EIDILTECK HPNIVELHE AFQKEQQLW 
MLIEYCDGG ALDSIMTEL EKPLTELQI AYVCQNMCK 
GLEFLHKNH VIHRDLKAG NVLLTMEGG VKLADFGVS 
AKNKSTMQK HDTFIGTPY WMAPEVVLC ETFRDNPYD 
FKVDVWSMG ITLIEFAQM EPPNHEMSP MRVLLKIQK 
SDPPKLEQP SKWSKEFND FVSNCLVKD PQKRPTCDE 
LLKHPFINC TLDSKPIRD LLLEYKAEV VEEELTDDD 
PEDNRTSHV PLDIEDDST SVKSSEVDN KVNDLESPV 
INVEEETAK DLTASTPSN VQADKKPLE KKDEVQRKT 
SRDKGKAPP PPIQPQASS STEKPPAPA APVAIPNLP 
TPPSSPITP PATPPSSSP VHPGSEPAK PEENKDNVD 
NIKEVLEKQ LKKRQSEGD VASEGVTVS ATSDDTVSN 
FVVVSSQSE RNKLNTSTI TINSDLPDP SNSLASNIS 
QVTVVTTHP PVIIDNTQC VSRSSTSPS NSTNEVVIV 
ANETNKTHV ESSDDECYP SLDSLEYPP PSEFRDRPL 
KAKKLDESE VLIVDSSYV INDSGLDVS DDNSRLLDT 
SHVSVVKID EEKVQVKDS TSYLDKSGD NFRSSTSDL 
SNASSCKTG SSDEVRGTS ITLDAPPNE IKAKMNGQV 
VLNGKGFDN YESALSDRS HSDCGSVRS NDSHRRPVS 
AKSIEHSDI ESISIASHE SRGSTGKEP KNDRLSELD 
ENDDGVILR KKAQKTKRT SLAIANRKT RKRTRKFII 
DGVVITTTT SKVICPDED NYNLADLHN DRKQELREL 
KYLQKQEQK QFQDLAFKE NLTLEQQDK KFEQERVNL 
ERTYENDLE MLSRQQRMQ IEKAEAHQD AELRAMSKK 
IRGEQEREL KIFRDGLKQ ELRLLKAEI DLLPKERRK 
AEFKLRKEK MDADHLERE KAFLEKLNE NHESSLRRL 
SDSHREKIA LMERQFLQQ KQQLTRTRE AALWEIEER 
QIHEKHQLM KRQIKEIFI LQRHQMLTR HEKEKEQIK 
RRAARKEEE LLKKQAAEK RSLPKRIRT EMKARELMF 
RESMRISIS GTNDPEAEK NKFKEFQEK EKKRYQAEQ 
QRFELKHQR QLEELRAMS DATIKELEQ LQNEKRKML 
LEHETMKLK QREEAFSRE LKEWKAQLK PRKQKLEEA 
FAQQLEEQE RVYGPISTP LALPPDLPD LSQESCKIG 
MGSTRSSLS SVSEG
    CDS sequence
    • >TC000382 RA
      atgtctttc ttcaacaac attaaaaaa gtgcttcat 
ttcggcggc aacgatgcg aaaaagaaa aaagtgttc 
aacaacgtg cgcatggag tgcgacccc gaggagttt 
tgggacatg atcggggag ctgggcgat ggggcttac 
ggcaaggtc tacaaagcc cagcataaa catacggga 
cagctggcc gcggcgaaa atgtgccga ctggatggg 
gaggacgac ctctccgac ttcatgata gaaattgat 
attttaacc gagtgcaaa catccaaat attgtcgag 
ttgcacgaa gccttccag aaagagcag caactctgg 
atgttaatc gaatactgt gatggtggg gccttagat 
agtattatg actgaatta gaaaaacct ctaactgaa 
ttacaaatt gcttatgtg tgccaaaac atgtgcaag 
ggactcgag ttcttgcac aaaaaccac gttatacac 
agggacttg aaagcggga aatgtgctg ctgaccatg 
gaaggaggg gtcaagctg gccgacttc ggagtctcc 
gctaaaaat aaaagcacg atgcaaaaa cacgacacg 
ttcatcgga acgccatac tggatggcc cccgaagtg 
gtcctttgt gagactttc cgcgacaat ccgtacgat 
ttcaaagtg gacgtgtgg tcgatgggc ataactctc 
attgaattc gcccaaatg gagccaccc aaccacgaa 
atgtcaccc atgagggtg ttactcaaa atccaaaaa 
tccgatccg cccaaattg gaacaaccg tcgaagtgg 
tcgaaggag tttaacgat ttcgtgtca aattgtctg 
gtcaaggat ccgcaaaaa agaccaacc tgtgacgag 
ttgttgaag catccgttt attaattgt acgctggac 
tcgaaaccg atcagggat ttgttgttg gagtacaag 
gcggaggtt gttgaagag gaattgaca gatgatgat 
cccgaggac aaccgcact tctcatgtt cctctggat 
atagaagat gactccact tctgtaaag agcagcgaa 
gttgacaat aaagtaaat gacctagaa tctcccgta 
attaatgta gaagaagaa actgctaaa gatttgaca 
gcttcgacg cctagcaat gtccaagcc gacaaaaaa 
ccgctggag aagaaagac gaagtccag aggaaaacc 
tcccgggat aaaggtaaa gcgccccca cctcccatc 
caaccccaa gcgagttcg tcgactgag aaacctcca 
gccccggct gcacctgtc gccattccc aacctacca 
acgccccca tcttcgccc atcacacct ccagcaact 
ccgccaagt tctagtcca gttcatcca ggtagcgaa 
cccgcaaaa cccgaagaa aacaaagac aacgtcgat 
aatattaaa gaagtatta gaaaaacaa ttgaagaag 
cggcagtct gaaggcgac gtagcgagt gagggagtc 
acagtcagt gcgacatct gacgacact gttagtaac 
tttgttgtg gtttcgtca caatctgaa cgaaacaaa 
ctaaacact tccactatc accattaac tctgatctt 
cccgatccc tcaaacagt ttagcgtcg aatatcagt 
caggtgact gtagtaacg actcatccg cccgttatt 
atcgacaat actcaatgc gtgtcgcga tcttcaact 
tcccccagt aatagtaca aacgaggtc gtgatagtt 
gcgaacgaa acgaataaa acacacgtt gaatcatca 
gacgatgaa tgttacccg agtttggat tcgttggaa 
tacccccca cccagcgag ttccgcgac aggccgctc 
aaagcgaaa aaattagac gaaagcgag gttttaatc 
gtcgattcg agttatgtt ataaacgat tcgggtctg 
gacgtatcc gacgataac tcccgactt ttggatact 
agtcacgtg tctgttgta aaaatcgac gaagagaaa 
gtccaagtc aaggattcg acgtcatac ctcgacaag 
tcgggtgat aatttccga agttcgacg agtgattta 
tcgaacgct tcctcgtgc aaaacggga agtagcgat 
gaagttcgg ggcacttcg ataacactc gacgcgccg 
ccgaacgaa attaaggca aaaatgaac ggacaagtt 
gtgctaaac ggcaaaggg ttcgacaat tacgagtcg 
gctttgtcc gaccgttca cacagcgac tgtggatcg 
gttcgcagc aacgactcg caccgaaga ccggtttcg 
gccaaaagt atcgagcat tcggacatt gaaagtatc 
tcaatagct agtcacgag agtcgggga agcaccggc 
aaagaaccg aagaacgac cgactgtcg gaattggac 
gagaacgac gacggtgtc atcttgcgc aagaaagcc 
cagaaaaca aaaagaacg tctctagca atcgccaat 
cgaaagacc cgcaaaaga acccggaaa tttataata 
gatggcgtt gtcattacc acgactacc agcaaagtg 
atttgtccc gacgaagat aattacaac ttggccgat 
ttgcataat gacaggaaa caagaattg cgcgaattg 
aaatacttg caaaaacag gaacaaaaa caattccag 
gatttggcg tttaaggag aatttaacg ctggaacag 
caggataaa aagttcgaa caagaacga gtgaatctc 
gaacggacg tacgaaaac gacttggag atgttgagt 
cggcagcag aggatgcaa atcgagaaa gccgaagcg 
catcaagat gccgaactc cgagctatg tcgaagaaa 
attcgaggg gagcaagaa cgcgagttg aagattttc 
cgagacggg cttaagcaa gaattgaga ttacttaaa 
gccgaaatc gatttgtta ccgaaggag cggcgcaag 
gccgaattt aaactgcga aaggagaaa atggatgct 
gatcatctc gagcgcgag aaggcgttt ttggagaaa 
ctcaatgag aatcatgag agttcgttg cgaaggctg 
agcgattcg catcgtgag aaaattgcg ttgatggaa 
aggcagttc ttgcagcag aagcagcag ttgacgagg 
acgcgcgaa gcggcgctt tgggagatc gaagagagg 
cagattcat gagaagcat cagttgatg aagagacag 
attaaggag atatttatt ctgcaaagg catcagatg 
ttgacgagg catgagaag gagaaggag cagattaag 
agacgggcg gcgaggaag gaagaggag ttgttaaag 
aaacaagcg gcggaaaaa aggtcgctg ccgaagagg 
atcaggacg gagatgaag gcgagagag ctgatgttc 
agggagtcg atgaggata tcaatttcc gggacgaac 
gatccggaa gcagagaag aataagttt aaagagttc 
caagagaag gagaaaaag cgctaccag gccgagcag 
caacgcttc gagctgaaa caccagcgc caactcgaa 
gagctccgc gcaatgagc gacgccacc atcaaagag 
ctcgaacaa ctccagaac gaaaagcgt aaaatgctc 
ctcgaacac gaaacgatg aaactaaag caacgcgaa 
gaagcattc agtcgcgaa ctaaaagaa tggaaagct 
caactcaaa ccgcgaaaa cagaaattg gaagaggcg 
tttgcccag cagttggaa gaacaggaa agggtttat 
gggccgatc agtactcct ctggctttg cctccagat 
ttgcccgat ctgagccag gagagttgc aagatcggg 
atggggtcg actaggagc agtttatcg tcggtttcg 
gagggatag
    mRNA sequence
    • >TC000382 RA
      tagggaaaa aatggtatg gctgacggt ttagtgaaa 
aatgaagat acacaaatg tgataaatc tgtggtgaa 
tttctgcag tgatgttat gaaaatttc ggtggtttt 
tgccttttt aaagctggt ttagattag caaagcaaa 
taaacaaaa atgaggaga agaaaatcg ctttcggac 
acctttgca gcgccttga gtgcagtgt ttgtgcgta 
atccgtgaa cattaaaag ccaaaatgt ctttcttca 
acaacatta aaaaagtgc ttcatttcg gcggcaacg 
atgcgaaaa agaaaaaag tgttcaaca acgtgcgca 
tggagtgcg accccgagg agttttggg acatgatcg 
gggagctgg gcgatgggg cttacggca aggtctaca 
aagcccagc ataaacata cgggacagc tggccgcgg 
cgaaaatgt gccgactgg atggggagg acgacctct 
ccgacttca tgatagaaa ttgatattt taaccgagt 
gcaaacatc caaatattg tcgagttgc acgaagcct 
tccagaaag agcagcaac tctggatgt taatcgaat 
actgtgatg gtggggcct tagatagta ttatgactg 
aattagaaa aacctctaa ctgaattac aaattgctt 
atgtgtgcc aaaacatgt gcaagggac tcgagttct 
tgcacaaaa accacgtta tacacaggg acttgaaag 
cgggaaatg tgctgctga ccatggaag gaggggtca 
agctggccg acttcggag tctccgcta aaaataaaa 
gcacgatgc aaaaacacg acacgttca tcggaacgc 
catactgga tggcccccg aagtggtcc tttgtgaga 
ctttccgcg acaatccgt acgatttca aagtggacg 
tgtggtcga tgggcataa ctctcattg aattcgccc 
aaatggagc cacccaacc acgaaatgt cacccatga 
gggtgttac tcaaaatcc aaaaatccg atccgccca 
aattggaac aaccgtcga agtggtcga aggagttta 
acgatttcg tgtcaaatt gtctggtca aggatccgc 
aaaaaagac caacctgtg acgagttgt tgaagcatc 
cgtttatta attgtacgc tggactcga aaccgatca 
gggatttgt tgttggagt acaaggcgg aggttgttg 
aagaggaat tgacagatg atgatcccg aggacaacc 
gcacttctc atgttcctc tggatatag aagatgact 
ccacttctg taaagagca gcgaagttg acaataaag 
taaatgacc tagaatctc ccgtaatta atgtagaag 
aagaaactg ctaaagatt tgacagctt cgacgccta 
gcaatgtcc aagccgaca aaaaaccgc tggagaaga 
aagacgaag tccagagga aaacctccc gggataaag 
gtaaagcgc ccccacctc ccatccaac cccaagcga 
gttcgtcga ctgagaaac ctccagccc cggctgcac 
ctgtcgcca ttcccaacc taccaacgc ccccatctt 
cgcccatca cacctccag caactccgc caagttcta 
gtccagttc atccaggta gcgaacccg caaaacccg 
aagaaaaca aagacaacg tcgataata ttaaagaag 
tattagaaa aacaattga agaagcggc agtctgaag 
gcgacgtag cgagtgagg gagtcacag tcagtgcga 
catctgacg acactgtta gtaactttg ttgtggttt 
cgtcacaat ctgaacgaa acaaactaa acacttcca 
ctatcacca ttaactctg atcttcccg atccctcaa 
acagtttag cgtcgaata tcagtcagg tgactgtag 
taacgactc atccgcccg ttattatcg acaatactc 
aatgcgtgt cgcgatctt caacttccc ccagtaata 
gtacaaacg aggtcgtga tagttgcga acgaaacga 
ataaaacac acgttgaat catcagacg atgaatgtt 
acccgagtt tggattcgt tggaatacc ccccaccca 
gcgagttcc gcgacaggc cgctcaaag cgaaaaaat 
tagacgaaa gcgaggttt taatcgtcg attcgagtt 
atgttataa acgattcgg gtctggacg tatccgacg 
ataactccc gacttttgg atactagtc acgtgtctg 
ttgtaaaaa tcgacgaag agaaagtcc aagtcaagg 
attcgacgt catacctcg acaagtcgg gtgataatt 
tccgaagtt cgacgagtg atttatcga acgcttcct 
cgtgcaaaa cgggaagta gcgatgaag ttcggggca 
cttcgataa cactcgacg cgccgccga acgaaatta 
aggcaaaaa tgaacggac aagttgtgc taaacggca 
aagggttcg acaattacg agtcggctt tgtccgacc 
gttcacaca gcgactgtg gatcggttc gcagcaacg 
actcgcacc gaagaccgg tttcggcca aaagtatcg 
agcattcgg acattgaaa gtatctcaa tagctagtc 
acgagagtc ggggaagca ccggcaaag aaccgaaga 
acgaccgac tgtcggaat tggacgaga acgacgacg 
gtgtcatct tgcgcaaga aagcccaga aaacaaaaa 
gaacgtctc tagcaatcg ccaatcgaa agacccgca 
aaagaaccc ggaaattta taatagatg gcgttgtca 
ttaccacga ctaccagca aagtgattt gtcccgacg 
aagataatt acaacttgg ccgatttgc ataatgaca 
ggaaacaag aattgcgcg aattgaaat acttgcaaa 
aacaggaac aaaaacaat tccaggatt tggcgttta 
aggagaatt taacgctgg aacagcagg ataaaaagt 
tcgaacaag aacgagtga atctcgaac ggacgtacg 
aaaacgact tggagatgt tgagtcggc agcagagga 
tgcaaatcg agaaagccg aagcgcatc aagatgccg 
aactccgag ctatgtcga agaaaattc gaggggagc 
aagaacgcg agttgaaga ttttccgag acgggctta 
agcaagaat tgagattac ttaaagccg aaatcgatt 
tgttaccga aggagcggc gcaaggccg aatttaaac 
tgcgaaagg agaaaatgg atgctgatc atctcgagc 
gcgagaagg cgtttttgg agaaactca atgagaatc 
atgagagtt cgttgcgaa ggctgagcg attcgcatc 
gtgagaaaa ttgcgttga tggaaaggc agttcttgc 
agcagaagc agcagttga cgaggacgc gcgaagcgg 
cgctttggg agatcgaag agaggcaga ttcatgaga 
agcatcagt tgatgaaga gacagatta aggagatat 
ttattctgc aaaggcatc agatgttga cgaggcatg 
agaaggaga aggagcaga ttaagagac gggcggcga 
ggaaggaag aggagttgt taaagaaac aagcggcgg 
aaaaaaggt cgctgccga agaggatca ggacggaga 
tgaaggcga gagagctga tgttcaggg agtcgatga 
ggatatcaa tttccggga cgaacgatc cggaagcag 
agaagaata agtttaaag agttccaag agaaggaga 
aaaagcgct accaggccg agcagcaac gcttcgagc 
tgaaacacc agcgccaac tcgaagagc tccgcgcaa 
tgagcgacg ccaccatca aagagctcg aacaactcc 
agaacgaaa agcgtaaaa tgctcctcg aacacgaaa 
cgatgaaac taaagcaac gcgaagaag cattcagtc 
gcgaactaa aagaatgga aagctcaac tcaaaccgc 
gaaaacaga aattggaag aggcgtttg cccagcagt 
tggaagaac aggaaaggg tttatgggc cgatcagta 
ctcctctgg ctttgcctc cagatttgc ccgatctga 
gccaggaga gttgcaaga tcgggatgg ggtcgacta 
ggagcagtt tatcgtcgg tttcggagg gatagaacg 
ctttaggaa acagtatac tccaaaatg tgcctctgc 
agatccaga gcgagcatt gattaccct aactcgtgt 
atatactta ttacacaga ctgaataat taagaaaat 
cgtatgaaa cattttttt cgttttgtt tcttttact 
agagagatt gaggtgacc tgataagct acttaaatt 
tggaaattt cgaaaggga gaatttttt tcaggttgt 
ttactgaaa acaaataaa ctattgtta gcttgcttg 
ctaaaacta gactagttt tacgtcgat cctttttat 
taaattaag attagaggt gattgagga tttaaaatt 
gtgtgcatt tattaaaac cagactgac aggttacac 
ttgttattt tgtgaaata attaagtta gattgtact 
aatagttca aaagattgt tatatcatg ttcgtaagt 
aagttttct ttgtattga ggtattatc taacaataa 
atactgatt aattattat ttaacataa tagattgta 
ttgggattg ttttttgtt tacgcttgc actactcca 
gtctgatgt ataaatact attatagtc catgttatt 
taaatactg aaattttta tcagctgta ttctaaggt 
gtacataca aattccatc taacttaag tttattagg 
acagtgtga taagatttt tatcgtctt ttagttctt 
tactaacgt ttgccacta tcatgctgt tctgtatta 
ttatgatgt acagtcagt gtattttat ttttatgtg 
catattatt taccagtaa ctattatat tcaaataaa 
taatataaa tagaa
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