Status
Please wait ...
Gene information
General information
TC032198
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000381
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12239680..12243617
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032198
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00082

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupation delayed (still prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032198 RA
      MASQRTQVQ RQQALALMC RVYVGSISF ELKEDTIRQ 
      SFLPFGPIK SINMSWDPV TQKHKGFAF VEYEIPEAA 
      QLALEQMNG VMIGGRNIK VVGRPSNMP QAQAVIDEI 
      QEEAKQYNR IYVASIHPD LTEDDIKSV FEAFGPIIY 
      CKLAQGSSG HKHKGYGFI EYETAQAAN EAIASMNLF 
      DLGGQYLRV GRAITPPNA LLGPSSGSS VMPTAAAVA 
      AAAATAKIQ AMDAVASNA AALGFSKLS QPVVAAAPP 
      PPVIPAVIP PPGLAIPQV LPDNTIQPT IVPVVTVPP 
      VIAPPTVVT PTLTQPAVP ARNSSEEAL KRAQEAQMK 
      QQEELQKKL LDQNEPQTL QQQENMSIK GQNARHFVM 
      QKLMRKVDS KVVILRNMV GPEDVDETL QEEIQEECS 
      KFGTVERVI IYNEKQSEE DDASDVVVK IFVEFTETP 
      EAEKARDAL NGRYFGGRM VQASLYDQT LFDHSDFSG
    • >TC032198 RB
      MEQSIRMVL MKQTLAHQQ QQMASQRTQ VQRQQALAL 
      MCRVYVGSI SFELKEDTI RQSFLPFGP IKSINMSWD 
      PVTQKHKGF AFVEYEIPE AAQLALEQM NGVMIGGRN 
      IKVVGRPSN MPQAQAVID EIQEEAKQY NRIYVASIH 
      PDLTEDDIK SVFEAFGPI IYCKLAQGS SGHKHKGYG 
      FIEYETAQA ANEAIASMN LFDLGGQYL RVGRAITPP 
      NALLGPSSG SSVMPTAAA VAAAAATAK IQAMDAVAS 
      NAAALGFSK LSQPVVAAA PPPPVIPAV IPPPGLAIP 
      QVLPDNTIQ PTIVPVVTV PPVIAPPTV VTPTLTQPA 
      VPARNSSEE ALKRAQEAQ MKQQEELQK KLLDQNEPQ 
      TLQQQENMS IKGQNARHF VMQKLMRKV DSKVVILRN 
      MVGPEDVDE TLQEEIQEE CSKFGTVER VIIYNEKQS 
      EEDDASDVV VKIFVEFTE TPEAEKARD ALNGRYFGG 
      RMVQASLYD QTLFDHSDF SG
    CDS sequence
    • >TC032198 RA
      atggctagt cagaggact caggtccag cgtcagcag 
      gctctcgcc cttatgtgc agggtgtac gttggaagc 
      atcagtttt gagctcaag gaggataca attaggcag 
      tccttttta ccatttgga ccaataaaa tccatcaat 
      atgtcgtgg gatccagtc acacaaaaa cacaaagga 
      tttgcattt gttgaatat gaaatacca gaagcagct 
      caattagcg ttagaacaa atgaacggc gttatgata 
      ggtggacgt aacattaaa gtcgtcgga cggcctagt 
      aacatgccc caagctcaa gccgttatt gacgaaata 
      caagaagaa gcaaaacag tacaaccgc atttatgtc 
      gcctccata catccagat ctgacagaa gacgacatc 
      aaaagcgta tttgaggca tttggtccc atcatatac 
      tgtaaactt gcgcaggga agctccggc cacaagcac 
      aaaggctat ggttttatc gagtacgag acggcacag 
      gcggccaac gaggcgatc gcaagcatg aatttattc 
      gaccttggg ggccagtat ttgcgcgtt ggccgcgcc 
      ataacgcct ccgaacgct ctcttaggc ccctcgtcc 
      ggttccagt gtgatgccc acggcggcg gcggtggcc 
      gcagccgcc gccacggcc aaaatccag gccatggac 
      gcggtggca agtaacgca gctgcgttg ggtttctcc 
      aaattgagt caaccggtg gtggcggct gcaccgccc 
      ccgccggtc atcccggcc gtaataccg cccccaggg 
      ctggcaata ccccaagtg cttccggat aatacgatt 
      caaccgacg attgtgccg gtggtgacg gtgcccccg 
      gtgattgcc cctccgacg gtggtcacc cccacgctg 
      acccagccg gcggtgcct gcgaggaat agctcggag 
      gaggcgttg aagcgggca caggaggcg cagatgaag 
      cagcaggag gagttgcag aagaagttg ttggatcag 
      aatgagccg cagacgttg caacagcag gagaatatg 
      agtataaag gggcaaaat gcgaggcat tttgttatg 
      cagaagttg atgcggaag gttgattcc aaagtggtg 
      attttgcgg aatatggta gggccggag gatgttgat 
      gagacgttg caagaggag attcaggag gagtgtagt 
      aaatttggg acggttgaa agagtgatt atctataac 
      gagaaacag tcggaagag gatgatgcg tccgatgtc 
      gtggttaaa atctttgta gagtttacg gaaacaccc 
      gaagcggaa aaggccaga gatgcactt aatgggagg 
      tactttggc ggaagaatg gttcaagca tcactttac 
      gaccaaact ttgtttgat catagtgat ttttcggga 
      taa
    • >TC032198 RB
      atggaacag agtatacgg atggtgctt atgaaacag 
      actctggcc catcaacaa cagcaaatg gctagtcag 
      aggactcag gtccagcgt cagcaggct ctcgccctt 
      atgtgcagg gtgtacgtt ggaagcatc agttttgag 
      ctcaaggag gatacaatt aggcagtcc tttttacca 
      tttggacca ataaaatcc atcaatatg tcgtgggat 
      ccagtcaca caaaaacac aaaggattt gcatttgtt 
      gaatatgaa ataccagaa gcagctcaa ttagcgtta 
      gaacaaatg aacggcgtt atgataggt ggacgtaac 
      attaaagtc gtcggacgg cctagtaac atgccccaa 
      gctcaagcc gttattgac gaaatacaa gaagaagca 
      aaacagtac aaccgcatt tatgtcgcc tccatacat 
      ccagatctg acagaagac gacatcaaa agcgtattt 
      gaggcattt ggtcccatc atatactgt aaacttgcg 
      cagggaagc tccggccac aagcacaaa ggctatggt 
      tttatcgag tacgagacg gcacaggcg gccaacgag 
      gcgatcgca agcatgaat ttattcgac cttgggggc 
      cagtatttg cgcgttggc cgcgccata acgcctccg 
      aacgctctc ttaggcccc tcgtccggt tccagtgtg 
      atgcccacg gcggcggcg gtggccgca gccgccgcc 
      acggccaaa atccaggcc atggacgcg gtggcaagt 
      aacgcagct gcgttgggt ttctccaaa ttgagtcaa 
      ccggtggtg gcggctgca ccgcccccg ccggtcatc 
      ccggccgta ataccgccc ccagggctg gcaataccc 
      caagtgctt ccggataat acgattcaa ccgacgatt 
      gtgccggtg gtgacggtg cccccggtg attgcccct 
      ccgacggtg gtcaccccc acgctgacc cagccggcg 
      gtgcctgcg aggaatagc tcggaggag gcgttgaag 
      cgggcacag gaggcgcag atgaagcag caggaggag 
      ttgcagaag aagttgttg gatcagaat gagccgcag 
      acgttgcaa cagcaggag aatatgagt ataaagggg 
      caaaatgcg aggcatttt gttatgcag aagttgatg 
      cggaaggtt gattccaaa gtggtgatt ttgcggaat 
      atggtaggg ccggaggat gttgatgag acgttgcaa 
      gaggagatt caggaggag tgtagtaaa tttgggacg 
      gttgaaaga gtgattatc tataacgag aaacagtcg 
      gaagaggat gatgcgtcc gatgtcgtg gttaaaatc 
      tttgtagag tttacggaa acacccgaa gcggaaaag 
      gccagagat gcacttaat gggaggtac tttggcgga 
      agaatggtt caagcatca ctttacgac caaactttg 
      tttgatcat agtgatttt tcgggataa
    mRNA sequence
    • >TC032198 RA
      cgcctccag aattaaaga aatattcta tttttatgg 
      tgaaacgtt gcaagatcg gtgtcttat tgactgaat 
      tgtggttgg tagtgcaat tatgatgac tacgtatca 
      gcaggagcg gctagtatt gaacggagg aacaaatgc 
      gcccgaacc catgagcac tcccgagga gtccgagcc 
      cgagcctcc ggcccccgg gaccgagga cccgccgcc 
      gaagatccc gaaactcga cagtaagta ttaactttg 
      aatcttgtt actagtaag gtatagagc ctcaagtac 
      gggacaccc atactaatg tgcgatgtc tgcttgaga 
      tgttccaga aatgagatc tgccagaac gtagcctca 
      cgtggagtt gaacgaaat gctgtaacc gtagcctac 
      acggttaca tggtaggaa aaccaaaga atttataca 
      tgtgtttga gtatgagtt tttggcgct gtttttgcg 
      gttgattgt gcgaaacac atactcatt caacattcg 
      tttgattat cgccaggac aatcgggtt gttttagac 
      gtatctatt ttctctttg tgtttccag atacagtta 
      ggtgcaacc agattcgag ttgttgcgt tactagagg 
      cttgacttg accgccgac tgcaaacta cgtgcaaga 
      acgacaaaa tgtacatat ttttctaag agataatta 
      taccagttt gtggcaggg tagtacacc ttgccagtt 
      tttgcaact ttctaatca caaaaaatg tgtgcgcat 
      tgcactgaa taacacgag attattgta gtctttttc 
      tccattgaa tatttaagt cacgatcat aacaaaacc 
      gtttattga gatacaaat attcagtaa ttgaacgct 
      aatttattt acttacatt acaaaacct taattaaaa 
      ttatacaaa aagtgtaag taaagtaag aacaaatct 
      acacaacct gaccatgat tttcgtaaa ttgtcaatt 
      attttgctc acattgaat tttgtttta gatggatca 
      caaaaagct agtttaact tatgatctt aaacagatt 
      ggagaagtt gtcacaggt aactaattt agttgattc 
      gtttgtttt tattaattt tggaatatg aaggaccag 
      gagctagga gtaatacat ttgcgggga tcctcggtg 
      gagctcttc caaagctgt ccacagacc agagcgaca 
      aggtcagca aggcgaaaa aatacgcca tggaacaga 
      gtatacgga tggtgctta tgaaacaga ctctggccc 
      atcaacaac aggtatatt tctttgaag ggaggaaaa 
      aattacaag ctaatgacg taaccagaa atataaaat 
      tgttggttt tctgattgc gttgttgtt gggtttttg 
      gatagattt ttaactgga agttgtttt attgtcaga 
      agggtcagg ctgttgagg actagactt agttacagt 
      ttggatttt attttgatt tagttgtcg cagtgtgtg 
      gttttacta atgagactt tgcttcaca gaaaaataa 
      gaccttcca gagacatca agtgttagt tttaatgtg 
      caggttagt gcgtagctt gtcgcacgc gcccgctca 
      gggggcgag agctcgccc ccaagcttg cgttttcta 
      gttgtgaca tgccacttg tcctctgtt gcagcaaat 
      ggctagtca gaggactca ggtccagcg tcagcaggc 
      tctcgccct tatgtgcag ggtgtacgt tggaagcat 
      cagttttga gctcaagga ggatacaat taggcagtc 
      ctttttacc atttggacc aataaaatc catcaatat 
      gtcgtggga tccagtcac acaaaaaca caaaggatt 
      tgcatttgt tgaatatga aataccaga agcagctca 
      attagcgtt agaacaaat gaacggcgt tatgatagg 
      tggacgtaa cattaaagt cgtcggacg gcctagtaa 
      catgcccca agctcaagc cgttattga cgaaataca 
      agaagaagc aaaacagta caaccgcat ttatgtcgc 
      ctccataca tccagatct gacagaaga cgacatcaa 
      aagcgtatt tgaggcatt tggtcccat catatactg 
      taaacttgc gcagggaag ctccggcca caagcacaa 
      aggctatgg ttttatcga gtacgagac ggcacaggc 
      ggccaacga ggcgatcgc aagcatgaa tttattcga 
      ccttggggg ccagtattt gcgcgttgg ccgcgccat 
      aacgcctcc gaacgctct cttaggccc ctcgtccgg 
      ttccagtgt gatgcccac ggcggcggc ggtggccgc 
      agccgccgc cacggccaa aatccaggc catggacgc 
      ggtggcaag taacgcagc tgcgttggg tttctccaa 
      attgagtca accggtggt ggcggctgc accgccccc 
      gccggtcat cccggccgt aataccgcc cccagggct 
      ggcaatacc ccaagtgct tccggataa tacgattca 
      accgacgat tgtgccggt ggtgacggt gcccccggt 
      gattgcccc tccgacggt ggtcacccc cacgctgac 
      ccagccggc ggtgcctgc gaggaatag ctcggagga 
      ggcgttgaa gcgggcaca ggaggcgca gatgaagca 
      gcaggagga gttgcagaa gaagttgtt ggatcagaa 
      tgagccgca gacgttgca acagcagga gaatatgag 
      tataaaggg gcaaaatgc gaggcattt tgttatgca 
      gaagttgat gcggaaggt tgattccaa agtggtgat 
      tttgcggaa tatggtagg gccggagga tgttgatga 
      gacgttgca agaggagat tcaggagga gtgtagtaa 
      atttgggac ggttgaaag agtgattat ctataacga 
      gaaacagtc ggaagagga tgatgcgtc cgatgtcgt 
      ggttaaaat ctttgtaga gtttacgga aacacccga 
      agcggaaaa ggccagaga tgcacttaa tgggaggta 
      ctttggcgg aagaatggt tcaagcatc actttacga 
      ccaaacttt gtttgatca tagtgattt ttcgggata 
      agttttagg aacgagtaa tgtttgttt ttctactgt 
      gtaggagta actgttctt gttaagtat taaacttta 
      aaagtttta tggtgcaaa ttgtgtact ttatttata 
      tcaattcaa ttttctaaa aaacctgta cattttgaa 
      atcattttg caatggaag atgtaactt tgccttttt 
      taaattgta aa
    • >TC032198 RB
      cgcctccag aattaaaga aatattcta tttttatgg 
      tgaaacgtt gcaagatcg gtgtcttat tgactgaat 
      tgtggttgg tagtgcaat tatgatgac tacgtatca 
      gcaggagcg gctagtatt gaacggagg aacaaatgc 
      gcccgaacc catgagcac tcccgagga gtccgagcc 
      cgagcctcc ggcccccgg gaccgagga cccgccgcc 
      gaagatccc gaaactcga cagtaagta ttaactttg 
      aatcttgtt actagtaag gtatagagc ctcaagtac 
      gggacaccc atactaatg tgcgatgtc tgcttgaga 
      tgttccaga aatgagatc tgccagaac gtagcctca 
      cgtggagtt gaacgaaat gctgtaacc gtagcctac 
      acggttaca tggtaggaa aaccaaaga atttataca 
      tgtgtttga gtatgagtt tttggcgct gtttttgcg 
      gttgattgt gcgaaacac atactcatt caacattcg 
      tttgattat cgccaggac aatcgggtt gttttagac 
      gtatctatt ttctctttg tgtttccag atacagtta 
      ggtgcaacc agattcgag ttgttgcgt tactagagg 
      cttgacttg accgccgac tgcaaacta cgtgcaaga 
      acgacaaaa tgtacatat ttttctaag agataatta 
      taccagttt gtggcaggg tagtacacc ttgccagtt 
      tttgcaact ttctaatca caaaaaatg tgtgcgcat 
      tgcactgaa taacacgag attattgta gtctttttc 
      tccattgaa tatttaagt cacgatcat aacaaaacc 
      gtttattga gatacaaat attcagtaa ttgaacgct 
      aatttattt acttacatt acaaaacct taattaaaa 
      ttatacaaa aagtgtaag taaagtaag aacaaatct 
      acacaacct gaccatgat tttcgtaaa ttgtcaatt 
      attttgctc acattgaat tttgtttta gatggatca 
      caaaaagct agtttaact tatgatctt aaacagatt 
      ggagaagtt gtcacaggt aactaattt agttgattc 
      gtttgtttt tattaattt tggaatatg aaggaccag 
      gagctagga gtaatacat ttgcgggga tcctcggtg 
      gagctcttc caaagctgt ccacagacc agagcgaca 
      aggtcagca aggcgaaaa aatacgcca tggaacaga 
      gtatacgga tggtgctta tgaaacaga ctctggccc 
      atcaacaac agcaaatgg ctagtcaga ggactcagg 
      tccagcgtc agcaggctc tcgccctta tgtgcaggg 
      tgtacgttg gaagcatca gttttgagc tcaaggagg 
      atacaatta ggcagtcct ttttaccat ttggaccaa 
      taaaatcca tcaatatgt cgtgggatc cagtcacac 
      aaaaacaca aaggatttg catttgttg aatatgaaa 
      taccagaag cagctcaat tagcgttag aacaaatga 
      acggcgtta tgataggtg gacgtaaca ttaaagtcg 
      tcggacggc ctagtaaca tgccccaag ctcaagccg 
      ttattgacg aaatacaag aagaagcaa aacagtaca 
      accgcattt atgtcgcct ccatacatc cagatctga 
      cagaagacg acatcaaaa gcgtatttg aggcatttg 
      gtcccatca tatactgta aacttgcgc agggaagct 
      ccggccaca agcacaaag gctatggtt ttatcgagt 
      acgagacgg cacaggcgg ccaacgagg cgatcgcaa 
      gcatgaatt tattcgacc ttgggggcc agtatttgc 
      gcgttggcc gcgccataa cgcctccga acgctctct 
      taggcccct cgtccggtt ccagtgtga tgcccacgg 
      cggcggcgg tggccgcag ccgccgcca cggccaaaa 
      tccaggcca tggacgcgg tggcaagta acgcagctg 
      cgttgggtt tctccaaat tgagtcaac cggtggtgg 
      cggctgcac cgcccccgc cggtcatcc cggccgtaa 
      taccgcccc cagggctgg caatacccc aagtgcttc 
      cggataata cgattcaac cgacgattg tgccggtgg 
      tgacggtgc ccccggtga ttgcccctc cgacggtgg 
      tcaccccca cgctgaccc agccggcgg tgcctgcga 
      ggaatagct cggaggagg cgttgaagc gggcacagg 
      aggcgcaga tgaagcagc aggaggagt tgcagaaga 
      agttgttgg atcagaatg agccgcaga cgttgcaac 
      agcaggaga atatgagta taaaggggc aaaatgcga 
      ggcattttg ttatgcaga agttgatgc ggaaggttg 
      attccaaag tggtgattt tgcggaata tggtagggc 
      cggaggatg ttgatgaga cgttgcaag aggagattc 
      aggaggagt gtagtaaat ttgggacgg ttgaaagag 
      tgattatct ataacgaga aacagtcgg aagaggatg 
      atgcgtccg atgtcgtgg ttaaaatct ttgtagagt 
      ttacggaaa cacccgaag cggaaaagg ccagagatg 
      cacttaatg ggaggtact ttggcggaa gaatggttc 
      aagcatcac tttacgacc aaactttgt ttgatcata 
      gtgattttt cgggataag ttttaggaa cgagtaatg 
      tttgttttt ctactgtgt aggagtaac tgttcttgt 
      taagtatta aactttaaa agttttatg gtgcaaatt 
      gtgtacttt atttatatc aattcaatt ttctaaaaa 
      acctgtaca ttttgaaat cattttgca atggaagat 
      gtaactttg cctttttta aattgtaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY