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Gene information
General information
TC000373 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 6762480..6764914
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000373
    iBeetle screen iB_00080
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupation delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000373 RA
      MMNISKVAL RSRVSRVKL TSQLAGLAT TPDEKIEIP 
      KRVPRGPTD ILRALESTV GRDPTAAHY KYHDDPYLI 
      PLSNNGKRV FALSQEAGR KAAHWIRRE HADLFQHRE 
      ADPTIEAFV PPMVFDEKS EVAVTDLQK LIKTAQVSD 
      AVVVYKLLK EKGAEVAPE LEQGLLELV CYQNCEDGL 
      SDEFIEEKW FKQSQKGKE KLRKTWKDG DFAEQLFMN 
      LPNKTPEAY CAIIQGMAK YYQVDRAVQ LFEEAQQNN 
      IPLNTNTYN SLISAVNIM KESFDLRWS LVAQFLNQM 
      KDAKLKPNL GTLNAILAA LSSMGNPVG ARKYALMTL 
      SQFRELGIE PSLGSWSLV LNTFYKDRG PVSTILHDI 
      INLVENKEH QIRDLKDVH FFVTAMETC RFYLNDVSL 
      AKRIDRLLH HGENYNLIG DSYKESVYY RHYFALLCA 
      NLPIEEFMN DVYYKLIPH IYVPEPALM AEVLNQVDV 
      NGAIEYVPK LWSDLVIFD HAHRENLIE MVLNSMTNN 
      EELLTPELN EKFATVGWD IFSRFDDQD SYKVSRMKV 
      ALSGDFLGK ILLLLARNK EFEKACCVM EKLDRNQQS 
      IAGVPKVEA LDKFVDLCI EEKAPSRAI ACIQYCVDC 
      GYGEANAMA VRLHKKLTL DEVFLDKLT KIVGDISLA 
      GAKDSV
    CDS sequence
    • >TC000373 RA
      atgatgaat atatctaaa gtagccctc cgttcgagg 
      gtgtctcgg gtcaagcta acctcacag ttagccggg 
      cttgcaacc acccctgat gaaaaaatc gaaattccg 
      aaacgtgtc ccccgtggc cccacggac atcctccgt 
      gcgttggag agtacagtg gggcgggac ccaaccgcc 
      gcccattac aaataccat gatgaccca tatctaatc 
      ccattatct aataacggg aagcgcgtt tttgcgttg 
      tcccaagag gccggtcgt aaagcggct cattggata 
      agacgcgaa catgccgat ttgtttcaa caccgggag 
      gccgatccc acgatagag gcctttgtg ccccctatg 
      gtctttgac gaaaaaagc gaagttgcc gttactgac 
      ctccagaag ctcataaaa acggctcaa gtgagtgac 
      gccgtcgtg gtttataag ctactgaag gagaaaggg 
      gctgaggtc gcccccgag ctggagcaa ggcttgctg 
      gagttagtc tgctaccag aattgtgag gacggcctc 
      tcggatgag tttatcgag gaaaagtgg ttcaaacag 
      tcgcagaag ggtaaggag aagttgagg aaaacgtgg 
      aaagatggc gattttgcg gaacaactt tttatgaac 
      ttgcctaat aaaacacca gaggcttat tgtgccata 
      atccagggc atggctaag tattatcag gtagatagg 
      gccgtgcaa ttgtttgag gaagcccaa caaaacaac 
      attcctctc aataccaac acttacaat tctttaatt 
      tctgccgtc aacattatg aaagaaagc ttcgactta 
      agatggtcc ctcgtagcc caatttctc aaccaaatg 
      aaagacgct aaacttaaa ccgaacttg gggacactt 
      aacgctata cttgccgca ctcagttcg atgggtaac 
      ccagtgggg gcgagaaaa tacgccctt atgacactg 
      tcccagttt agagaattg gggattgag ccttcgcta 
      ggctcgtgg agtttggtt ttgaatact ttttacaaa 
      gacagggga ccagtgagt acaattttg cacgatata 
      attaattta gtagagaat aaagaacac caaattcgg 
      gatttgaaa gacgttcat ttttttgta acggctatg 
      gaaacttgt cggttctac ttgaacgac gtctctttg 
      gccaaacgc atcgatcgg ctgttacat cacggcgaa 
      aattataac ttaattggg gattcttac aaagaatcg 
      gtctattat cgtcattac tttgccctt ctttgcgcg 
      aatctgccc atcgaagaa ttcatgaat gacgtttac 
      tacaaatta ataccccac atttacgta cccgagcct 
      gcactaatg gccgaagtg ctcaaccaa gtcgacgtt 
      aacggtgcc attgaatac gtgccaaaa ttatggtca 
      gacttggtt attttcgat cacgcccat cgtgaaaat 
      cttattgaa atggttctg aactcgatg acaaacaac 
      gaggagctt ctcactcct gaattgaac gaaaaattc 
      gccacagtc gggtgggac attttttcg aggtttgac 
      gatcaggat agttataaa gttagcaga atgaaggtt 
      gcattgtcg ggtgatttt ctggggaaa attttactg 
      ttattagcg agaaacaaa gagtttgag aaagcgtgt 
      tgcgtcatg gagaagttg gatagaaat cagcaaagt 
      atcgctgga gtgccgaaa gttgaagcg ttggacaag 
      tttgtcgat ttgtgcatt gaggagaaa gcgccctca 
      cgggctatt gcctgtatc cagtattgc gtcgattgt 
      ggctatggg gaggctaac gccatggcc gtcagactt 
      cacaaaaag ctcactttg gacgaagtt ttcctcgac 
      aagcttacg aaaatcgtg ggcgacatc tcactagct 
      ggtgctaag gactctgtt taa
    mRNA sequence
    • >TC000373 RA
      gcgctagta aaatgatga atatatcta aagtagccc 
      tccgttcga gggtgtctc gggtcaagc taacctcac 
      agttagccg ggcttgcaa ccacccctg atgaaaaaa 
      tcgaaattc cgaaacgtg tcccccgtg gccccacgg 
      acatcctcc gtgcgttgg agagtacag tggggcggg 
      acccaaccg ccgcccatt acaaatacc atgatgacc 
      catatctaa tcccattat ctaataacg ggaagcgcg 
      tttttgcgt tgtcccaag aggccggtc gtaaagcgg 
      ctcattgga taagacgcg aacatgccg atttgtttc 
      aacaccggg aggccgatc ccacgatag aggcctttg 
      tgcccccta tggtctttg acgaaaaaa gcgaagttg 
      ccgttactg acctccaga agctcataa aaacggctc 
      aagtgagtg acgccgtcg tggtttata agctactga 
      aggagaaag gggctgagg tcgcccccg agctggagc 
      aaggcttgc tggagttag tctgctacc agaattgtg 
      aggacggcc tctcggatg agtttatcg aggaaaagt 
      ggttcaaac agtcgcaga agggtaagg agaagttga 
      ggaaaacgt ggaaagatg gcgattttg cggaacaac 
      tttttatga acttgccta ataaaacac cagaggctt 
      attgtgcca taatccagg gcatggcta agtattatc 
      aggtagata gggccgtgc aattgtttg aggaagccc 
      aacaaaaca acattcctc tcaatacca acacttaca 
      attctttaa tttctgccg tcaacatta tgaaagaaa 
      gcttcgact taagatggt ccctcgtag cccaatttc 
      tcaaccaaa tgaaagacg ctaaactta aaccgaact 
      tggggacac ttaacgcta tacttgccg cactcagtt 
      cgatgggta acccagtgg gggcgagaa aatacgccc 
      ttatgacac tgtcccagt ttagagaat tggggattg 
      agccttcgc taggctcgt ggagtttgg ttttgaata 
      ctttttaca aagacaggg gaccagtga gtacaattt 
      tgcacgata taattaatt tagtagaga ataaagaac 
      accaaattc gggatttga aagacgttc atttttttg 
      taacggcta tggaaactt gtcggttct acttgaacg 
      acgtctctt tggccaaac gcatcgatc ggctgttac 
      atcacggcg aaaattata acttaattg gggattctt 
      acaaagaat cggtctatt atcgtcatt actttgccc 
      ttctttgcg cgaatctgc ccatcgaag aattcatga 
      atgacgttt actacaaat taatacccc acatttacg 
      tacccgagc ctgcactaa tggccgaag tgctcaacc 
      aagtcgacg ttaacggtg ccattgaat acgtgccaa 
      aattatggt cagacttgg ttattttcg atcacgccc 
      atcgtgaaa atcttattg aaatggttc tgaactcga 
      tgacaaaca acgaggagc ttctcactc ctgaattga 
      acgaaaaat tcgccacag tcgggtggg acatttttt 
      cgaggtttg acgatcagg atagttata aagttagca 
      gaatgaagg ttgcattgt cgggtgatt ttctgggga 
      aaattttac tgttattag cgagaaaca aagagtttg 
      agaaagcgt gttgcgtca tggagaagt tggatagaa 
      atcagcaaa gtatcgctg gagtgccga aagttgaag 
      cgttggaca agtttgtcg atttgtgca ttgaggaga 
      aagcgccct cacgggcta ttgcctgta tccagtatt 
      gcgtcgatt gtggctatg gggaggcta acgccatgg 
      ccgtcagac ttcacaaaa agctcactt tggacgaag 
      ttttcctcg acaagctta cgaaaatcg tgggcgaca 
      tctcactag ctggtgcta aggactctg tttaagttg 
      tagttttct tgtaataaa acaacgttt aagta