General information | |||
TC000357 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 12568195..12570336 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC000357 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00078 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
abdomen musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
low validity due to technical reasons - should be repeated: stink gland phenotype not scorable, adult phenotype not scorable, lethality not scorable,
adult progeny in the well |
adult progeny in the well |
- >TC000357 RA
MPTCTSESL SRVWPSNES IHAGSISSS SGIQSPSSP NSLSPISTS PDNTLRRRR IRRAASLGT VIQEPFVIR SDCRDSRSQ SPVLNQREE EDDSFSVLA DIIGWYPCF LLRIAKAVI LAVLPRTWR QLIFALPML WISMWVWFF TQLINLPLN IIKLMLSIV LSPSSERTR KKRTVLISG GSTIQALHL ARNFYSAGA RVVVCEIDG LFALTRFST AVSRFYTVP KPTSDQQQN YVRALCEIV DKEQAMYYI PVSSTTSAY FDAVAKPHL ELLGCTCFC PGIKEVWAL DDTLEVLKR CQKYSIPTP TYHAITSKD DVYQLYDTG AIRMGRYVM TTAGPSGLR ERSKIILPV ARNDLKLPN DISEQRPWV VVQDVEGEH YLTCTTVKQ SQVIANVTC RMDKSSNGL VPIENKEIN AWLTTFFGK LNLLRPITG HVSFRFVIP KDSTTIIPL SSRVGVSLP YICYTNVHP RLVWKPCKH FSRQNSGPL VSENGRYWM GETVMTTIR NPNLEAVTR LIGTVIDKR EALFAIWDP LPYCAYYHM LLPFKNMLG FMQRRQDSF PRPMTNPIR
- >TC000357 RA
atgcccact tgtacttcg gaaagttta tcacgagtg tggccaagc aatgagagc atccatgcc gggagcatc agcagcagc tcggggatc cagagtccc agctccccc aactcccta tccccgatc tccacctcc ccggacaac acgctccgg agacggcgg atacgacgc gccgcgtcc ctcggcacc gtcatccag gagcccttc gtcatccgc tcggattgt cgcgactcg cggagccaa agtcccgtc ctgaaccag agggaggag gaggatgac tccttctcg gtcctggcg gacatcatc ggctggtac ccgtgcttt ctgctgcgc atcgccaag gccgtgatt ttggccgtt ttgccccgc acttggaga caactcatt ttcgctctc cccatgttg tggatctca atgtgggtc tggttcttc acgcagttg atcaatctg ccgctgaat ataatcaaa ctgatgctc tctatagtc ctatcaccg agtagtgaa aggactagg aagaaacga acggttttg ataagtggc gggagcacg atccaggcg cttcacttg gcccggaat ttctactcg gcgggggcg agagtcgtg gtttgcgaa atcgacggt ttgttcgcc ttaactcgc ttttctacc gctgtgagt cggttttac acggtcccg aaacccaca agtgaccaa caacaaaac tacgtgagg gcactttgc gaaatcgtg gataaggaa caagcgatg tattatatc cctgttagt tccaccacc tcggcgtac tttgacgcg gtggcgaag ccacacttg gaactcctg gggtgcact tgtttctgc cccggaatc aaagaagtg tgggctttg gacgacacg ttagaagtc ctcaaacgt tgccagaaa tactcaatt ccgaccccg acttaccac gcaatcacc tcaaaagac gatgtgtac cagttgtac gacacgggg gccattcga atgggacgc tacgttatg acaacagcg ggccccagt ggcctccgc gagcgctca aaaatcata ctcccggtc gcacgaaac gacctaaaa ctcccgaat gatataagc gagcagcgg ccttgggtt gtggtccag gacgtcgaa ggggagcat tatttgact tgcaccacc gtcaagcaa tcgcaagtt atcgctaac gtgacttgc cgcatggat aagtcgagc aatggactt gtcccgatc gaaaacaag gaaattaac gcgtggctg acgaccttc ttcggcaaa ttgaacctt ctaaggccc ataaccggt catgtcagc ttccgattt gtcatcccg aaagacagt acaacgatt atcccgttg agtagccgc gtgggggtc tcattgccg tacatttgc tacacgaac gtccatccc agattggtt tggaagccg tgcaagcac ttcagccgg cagaactcc gggccgttg gtcagcgaa aacgggagg tactggatg ggggagacc gtcatgacg acgatcagg aacccgaac cttgaggcc gtgacgagg ctgatcggg acggtcatc gacaagagg gaggcgctg ttcgccatt tgggacccc ttgccctac tgtgcctac taccacatg ttgttgcct ttcaagaac atgctgggg ttcatgcag cggcggcag gacagcttt cccaggccg atgactaac ccgatccgc taa
- >TC000357 RA
gaacgtgat tcttcgttc gtcgaatgg aaggggtac tcggaacca aggggcata gtacaacat gaagcttat aagagacct gtgcctcgt cactgtccg tttattaaa ttaatgact cgacggtgg cacacatct caagtgctt gaaattgaa ttagtgtct gtgcaagat gcccacttg tacttcgga aagtttatc acgagtgtg gccaagcaa tgagagcat ccatgccgg gagcatcag cagcagctc ggggatcca gagtcccag ctcccccaa ctccctatc cccgatctc cacctcccc ggacaacac gctccggag acggcggat acgacgcgc cgcgtccct cggcaccgt catccagga gcccttcgt catccgctc ggattgtcg cgactcgcg gagccaaag tcccgtcct gaaccagag ggaggagga ggatgactc cttctcggt cctggcgga catcatcgg ctggtaccc gtgctttct gctgcgcat cgccaaggc cgtgatttt ggccgtttt gccccgcac ttggagaca actcatttt cgctctccc catgttgtg gatctcaat gtgggtctg gttcttcac gcagttgat caatctgcc gctgaatat aatcaaact gatgctctc tatagtcct atcaccgag tagtgaaag gactaggaa gaaacgaac ggttttgat aagtggcgg gagcacgat ccaggcgct tcacttggc ccggaattt ctactcggc gggggcgag agtcgtggt ttgcgaaat cgacggttt gttcgcctt aactcgctt ttctaccgc tgtgagtcg gttttacac ggtcccgaa acccacaag tgaccaaca acaaaacta cgtgagggc actttgcga aatcgtgga taaggaaca agcgatgta ttatatccc tgttagttc caccacctc ggcgtactt tgacgcggt ggcgaagcc acacttgga actcctggg gtgcacttg tttctgccc cggaatcaa agaagtgtg ggctttgga cgacacgtt agaagtcct caaacgttg ccagaaata ctcaattcc gaccccgac ttaccacgc aatcacctc aaaagacga tgtgtacca gttgtacga cacgggggc cattcgaat gggacgcta cgttatgac aacagcggg ccccagtgg cctccgcga gcgctcaaa aatcatact cccggtcgc acgaaacga cctaaaact cccgaatga tataagcga gcagcggcc ttgggttgt ggtccagga cgtcgaagg ggagcatta tttgacttg caccaccgt caagcaatc gcaagttat cgctaacgt gacttgccg catggataa gtcgagcaa tggacttgt cccgatcga aaacaagga aattaacgc gtggctgac gaccttctt cggcaaatt gaaccttct aaggcccat aaccggtca tgtcagctt ccgatttgt catcccgaa agacagtac aacgattat cccgttgag tagccgcgt gggggtctc attgccgta catttgcta cacgaacgt ccatcccag attggtttg gaagccgtg caagcactt cagccggca gaactccgg gccgttggt cagcgaaaa cgggaggta ctggatggg ggagaccgt catgacgac gatcaggaa cccgaacct tgaggccgt gacgaggct gatcgggac ggtcatcga caagaggga ggcgctgtt cgccatttg ggacccctt gccctactg tgcctacta ccacatgtt gttgccttt caagaacat gctggggtt catgcagcg gcggcagga cagctttcc caggccgat gactaaccc gatccgcta atagtttca tctcattag gagagagaa aacagcctg agtattgtg tgtgtatgg actgtgcat tatttattc tggttacca aaggttgca tttatttat agataatgt gccattcgt ttgtatttt tttaagttt tgtacaaat attgttatt gtatatatt gtgtatata caactagta tgttacatt aactcgcgg gtaccacct ggctagtgt agtaccaag ttttgtaat ttgtggttt ttattttaa taaaaatat cagtcgaga
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
