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Gene information
General information
TC000357 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12568195..12570336
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000357
    iBeetle screen iB_00078

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    low validity due to technical reasons - should be repeated: stink gland phenotype not scorable, adult phenotype not scorable, lethality not scorable,
    adult progeny in the well
    Adult morphology
    low validity due to technical reasons - should be repeated: stink gland phenotype not scorable, adult phenotype not scorable, lethality not scorable,
    adult progeny in the well
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000357 RA
      MPTCTSESL SRVWPSNES IHAGSISSS SGIQSPSSP 
      NSLSPISTS PDNTLRRRR IRRAASLGT VIQEPFVIR 
      SDCRDSRSQ SPVLNQREE EDDSFSVLA DIIGWYPCF 
      LLRIAKAVI LAVLPRTWR QLIFALPML WISMWVWFF 
      TQLINLPLN IIKLMLSIV LSPSSERTR KKRTVLISG 
      GSTIQALHL ARNFYSAGA RVVVCEIDG LFALTRFST 
      AVSRFYTVP KPTSDQQQN YVRALCEIV DKEQAMYYI 
      PVSSTTSAY FDAVAKPHL ELLGCTCFC PGIKEVWAL 
      DDTLEVLKR CQKYSIPTP TYHAITSKD DVYQLYDTG 
      AIRMGRYVM TTAGPSGLR ERSKIILPV ARNDLKLPN 
      DISEQRPWV VVQDVEGEH YLTCTTVKQ SQVIANVTC 
      RMDKSSNGL VPIENKEIN AWLTTFFGK LNLLRPITG 
      HVSFRFVIP KDSTTIIPL SSRVGVSLP YICYTNVHP 
      RLVWKPCKH FSRQNSGPL VSENGRYWM GETVMTTIR 
      NPNLEAVTR LIGTVIDKR EALFAIWDP LPYCAYYHM 
      LLPFKNMLG FMQRRQDSF PRPMTNPIR
    CDS sequence
    • >TC000357 RA
      atgcccact tgtacttcg gaaagttta tcacgagtg 
      tggccaagc aatgagagc atccatgcc gggagcatc 
      agcagcagc tcggggatc cagagtccc agctccccc 
      aactcccta tccccgatc tccacctcc ccggacaac 
      acgctccgg agacggcgg atacgacgc gccgcgtcc 
      ctcggcacc gtcatccag gagcccttc gtcatccgc 
      tcggattgt cgcgactcg cggagccaa agtcccgtc 
      ctgaaccag agggaggag gaggatgac tccttctcg 
      gtcctggcg gacatcatc ggctggtac ccgtgcttt 
      ctgctgcgc atcgccaag gccgtgatt ttggccgtt 
      ttgccccgc acttggaga caactcatt ttcgctctc 
      cccatgttg tggatctca atgtgggtc tggttcttc 
      acgcagttg atcaatctg ccgctgaat ataatcaaa 
      ctgatgctc tctatagtc ctatcaccg agtagtgaa 
      aggactagg aagaaacga acggttttg ataagtggc 
      gggagcacg atccaggcg cttcacttg gcccggaat 
      ttctactcg gcgggggcg agagtcgtg gtttgcgaa 
      atcgacggt ttgttcgcc ttaactcgc ttttctacc 
      gctgtgagt cggttttac acggtcccg aaacccaca 
      agtgaccaa caacaaaac tacgtgagg gcactttgc 
      gaaatcgtg gataaggaa caagcgatg tattatatc 
      cctgttagt tccaccacc tcggcgtac tttgacgcg 
      gtggcgaag ccacacttg gaactcctg gggtgcact 
      tgtttctgc cccggaatc aaagaagtg tgggctttg 
      gacgacacg ttagaagtc ctcaaacgt tgccagaaa 
      tactcaatt ccgaccccg acttaccac gcaatcacc 
      tcaaaagac gatgtgtac cagttgtac gacacgggg 
      gccattcga atgggacgc tacgttatg acaacagcg 
      ggccccagt ggcctccgc gagcgctca aaaatcata 
      ctcccggtc gcacgaaac gacctaaaa ctcccgaat 
      gatataagc gagcagcgg ccttgggtt gtggtccag 
      gacgtcgaa ggggagcat tatttgact tgcaccacc 
      gtcaagcaa tcgcaagtt atcgctaac gtgacttgc 
      cgcatggat aagtcgagc aatggactt gtcccgatc 
      gaaaacaag gaaattaac gcgtggctg acgaccttc 
      ttcggcaaa ttgaacctt ctaaggccc ataaccggt 
      catgtcagc ttccgattt gtcatcccg aaagacagt 
      acaacgatt atcccgttg agtagccgc gtgggggtc 
      tcattgccg tacatttgc tacacgaac gtccatccc 
      agattggtt tggaagccg tgcaagcac ttcagccgg 
      cagaactcc gggccgttg gtcagcgaa aacgggagg 
      tactggatg ggggagacc gtcatgacg acgatcagg 
      aacccgaac cttgaggcc gtgacgagg ctgatcggg 
      acggtcatc gacaagagg gaggcgctg ttcgccatt 
      tgggacccc ttgccctac tgtgcctac taccacatg 
      ttgttgcct ttcaagaac atgctgggg ttcatgcag 
      cggcggcag gacagcttt cccaggccg atgactaac 
      ccgatccgc taa
    mRNA sequence
    • >TC000357 RA
      gaacgtgat tcttcgttc gtcgaatgg aaggggtac 
      tcggaacca aggggcata gtacaacat gaagcttat 
      aagagacct gtgcctcgt cactgtccg tttattaaa 
      ttaatgact cgacggtgg cacacatct caagtgctt 
      gaaattgaa ttagtgtct gtgcaagat gcccacttg 
      tacttcgga aagtttatc acgagtgtg gccaagcaa 
      tgagagcat ccatgccgg gagcatcag cagcagctc 
      ggggatcca gagtcccag ctcccccaa ctccctatc 
      cccgatctc cacctcccc ggacaacac gctccggag 
      acggcggat acgacgcgc cgcgtccct cggcaccgt 
      catccagga gcccttcgt catccgctc ggattgtcg 
      cgactcgcg gagccaaag tcccgtcct gaaccagag 
      ggaggagga ggatgactc cttctcggt cctggcgga 
      catcatcgg ctggtaccc gtgctttct gctgcgcat 
      cgccaaggc cgtgatttt ggccgtttt gccccgcac 
      ttggagaca actcatttt cgctctccc catgttgtg 
      gatctcaat gtgggtctg gttcttcac gcagttgat 
      caatctgcc gctgaatat aatcaaact gatgctctc 
      tatagtcct atcaccgag tagtgaaag gactaggaa 
      gaaacgaac ggttttgat aagtggcgg gagcacgat 
      ccaggcgct tcacttggc ccggaattt ctactcggc 
      gggggcgag agtcgtggt ttgcgaaat cgacggttt 
      gttcgcctt aactcgctt ttctaccgc tgtgagtcg 
      gttttacac ggtcccgaa acccacaag tgaccaaca 
      acaaaacta cgtgagggc actttgcga aatcgtgga 
      taaggaaca agcgatgta ttatatccc tgttagttc 
      caccacctc ggcgtactt tgacgcggt ggcgaagcc 
      acacttgga actcctggg gtgcacttg tttctgccc 
      cggaatcaa agaagtgtg ggctttgga cgacacgtt 
      agaagtcct caaacgttg ccagaaata ctcaattcc 
      gaccccgac ttaccacgc aatcacctc aaaagacga 
      tgtgtacca gttgtacga cacgggggc cattcgaat 
      gggacgcta cgttatgac aacagcggg ccccagtgg 
      cctccgcga gcgctcaaa aatcatact cccggtcgc 
      acgaaacga cctaaaact cccgaatga tataagcga 
      gcagcggcc ttgggttgt ggtccagga cgtcgaagg 
      ggagcatta tttgacttg caccaccgt caagcaatc 
      gcaagttat cgctaacgt gacttgccg catggataa 
      gtcgagcaa tggacttgt cccgatcga aaacaagga 
      aattaacgc gtggctgac gaccttctt cggcaaatt 
      gaaccttct aaggcccat aaccggtca tgtcagctt 
      ccgatttgt catcccgaa agacagtac aacgattat 
      cccgttgag tagccgcgt gggggtctc attgccgta 
      catttgcta cacgaacgt ccatcccag attggtttg 
      gaagccgtg caagcactt cagccggca gaactccgg 
      gccgttggt cagcgaaaa cgggaggta ctggatggg 
      ggagaccgt catgacgac gatcaggaa cccgaacct 
      tgaggccgt gacgaggct gatcgggac ggtcatcga 
      caagaggga ggcgctgtt cgccatttg ggacccctt 
      gccctactg tgcctacta ccacatgtt gttgccttt 
      caagaacat gctggggtt catgcagcg gcggcagga 
      cagctttcc caggccgat gactaaccc gatccgcta 
      atagtttca tctcattag gagagagaa aacagcctg 
      agtattgtg tgtgtatgg actgtgcat tatttattc 
      tggttacca aaggttgca tttatttat agataatgt 
      gccattcgt ttgtatttt tttaagttt tgtacaaat 
      attgttatt gtatatatt gtgtatata caactagta 
      tgttacatt aactcgcgg gtaccacct ggctagtgt 
      agtaccaag ttttgtaat ttgtggttt ttattttaa 
      taaaaatat cagtcgaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY