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Gene information
General information
TC000335 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12970922..12975542
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000335
    iBeetle screen iB_00076

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000335 RA
      MKMTLSSTN GTTNEKLTT PTENKQGSP NSSPRASPR 
      PSPRPQPKR EHPKSETHL TVNYKEIPG SNKHDGSPS 
      SQESSVSSR ASNLDISPP SNLKVESTS SSNKERKDP 
      RGKKKSSWF NPFYPTYKS RSEDFKRIF KDVPDDERL 
      LVDYSCALQ KEILAQGRL YVTQNYLCF YANILGWET 
      TLKLKWKDV SAITKEKTA IVIPNAVLI CTRTEKYFF 
      TSFVARDKT YLMLFRVWQ NALMDQPMS PQEMWQWVH 
      QWYGEELGL TSDDEDYVA PGTEEDKLS ARLSVESFS 
      EHESSAVEN PLEAMVVEE KPPQDDDSV TNRPVDRRR 
      TTSSHLPTD YSDTTESDG EKQTKTKPT FFLSWDKSS 
      PFLHPSSPE YDDTVPVLP NVKCDTHVQ CTSTHEGRQ 
      IMNEIVPIH IDQLFTLLF TSSKFYLDF HASRKTTDL 
      TQTPWTHNP SDNSKTRVV NLTVALTQA MGPKTAQVT 
      ETQVMLPCS KAGCLYAID VDTVNAGIP YADSFNIVV 
      HHCLQKISE TESSYQVFA QVMYKKSVW GLVKGMIEK 
      TCWSGLEDF YVSLAKALH VEGEENVCD VKRKSRRKR 
      RIHSMPRPG VEDSRPLIG ASSRISSGG IFSSDVATM 
      IVFTVLILL VVLNVMLYF KLWSLEESP PYTLLDLHV 
      LKDPPKTHD EWIKLLQQQ ETLHSVELQ KWQRVLKTA 
      IQLLKQTEE SLNELQRSI NPSYSSKVM SILQNHKDT 
      VEQNEKSEL
    • >TC000335 RB
      MKMTLSSTN GTTNEKLTT PTENKQGSP NSSPRASPR 
      PSPRPQPKR EHPKSETHL TVNYKEIPG SNKHDGSPS 
      SQESSVSSR ASNLDISPP SNLKVESTS SSNKERKDP 
      RGKKKSSWF NPFYPTYKS RSEDFKRIF KDVPDDERL 
      LVDYSCALQ KEILAQGRL YVTQNYLCF YANILGWET 
      TLKLKWKDV SAITKEKTA IVIPNAVLI CTRTEKYFF 
      TSFVARDKT YLMLFRVWQ NALMDQPMS PQEMWQWVH 
      QWYGEELGL TSDDEDYVA PGTEEDKLS ARLSVESFS 
      EHESSAVEN PLEAMVVEE KPPQDDDSV TNRPVDRRR 
      TTSSHLPTD YSDTTESDG EKQTKCDTH VQCTSTHEG 
      RQIMNEIVP IHIDQLFTL LFTSSKFYL DFHASRKTT 
      DLTQTPWTH NPSDNSKTR VVNLTVALT QAMGPKTAQ 
      VTETQVMLP CSKAGCLYA IDVDTVNAG IPYADSFNI 
      VVHHCLQKI SETESSYQV FAQVMYKKS VWGLVKGMI 
      EKTCWSGLE DFYVSLAKA LHVEGEENV CDVKRKSRR 
      KRRIHSMPR PGVEDSRPL IGASSRISS GGIFSSDVA 
      TMIVFTVLI LLVVLNVML YFKLWSLEE SPPYTLLDL 
      HVLKDPPKT HDEWIKLLQ QQETLHSVE LQKWQRVLK 
      TAIQLLKQT EESLNELQR SINPSYSSK VMSILQNHK 
      DTVEQNEKS EL
    CDS sequence
    • >TC000335 RA
      atgaagatg acattatcg tcgacaaat ggtacaacg 
      aacgaaaag ttaaccaca ccgacagaa aacaagcaa 
      ggcagcccc aattctagc cctcgggct agccccaga 
      ccgtccccg aggccgcag ccgaaacgg gaacacccc 
      aaaagtgag acgcacctc accgtcaat tacaaggaa 
      atccccgga agtaacaag cacgatggg tccccttct 
      agtcaggag agttctgta agtagtcgc gccagtaat 
      ttggacatt agcccgccc tcgaacttg aaagttgag 
      agcaccagt agctcgaat aaagaacgt aaggaccca 
      cgtggtaag aagaagtcg tcttggttc aatcccttc 
      tacccgacc tacaagtca cgatcggag gattttaag 
      agaattttt aaggacgta cctgacgat gagcgtttg 
      ctagtcgat tattcctgc gctttgcaa aaagaaata 
      ttggcacaa ggcagactc tacgtcacg caaaactat 
      ctctgcttt tacgcgaat attctggga tgggagacc 
      acacttaag ctaaaatgg aaggacgta tcagccata 
      accaaagag aaaactgca atcgtaatc cccaacgcg 
      gttttgatt tgcacaagg acggaaaag tactttttc 
      accagtttt gtagcacgg gacaagacg tatttgatg 
      ctgtttcga gtatggcag aacgcgctc atggaccaa 
      cctatgtct ccacaggag atgtggcaa tgggtgcac 
      cagtggtat ggtgaagag ctgggcttg accagtgat 
      gatgaggac tacgtagcc cccggtact gaagaggac 
      aaactatcg gcacgctta tctgtcgaa tctttctct 
      gagcacgag agcagcgct gttgagaac ccactggaa 
      gcgatggtg gtggaggag aagccccca caagacgac 
      gacagcgtc accaatagg ccggtggat cgccgccga 
      actacttct tcgcatctc cctactgat tactccgat 
      acgaccgaa tcggacgga gaaaaacaa acgaaaact 
      aaaccgacc tttttccta tcctgggat aaatcctct 
      cctttttta cacccttca agtcccgaa tatgacgat 
      actgttccg gttttgccg aatgtcaaa tgtgataca 
      cacgtacag tgcacttcg actcacgaa ggtcgtcaa 
      ataatgaac gaaatcgta cctatccat atagatcaa 
      ttatttact ctacttttt acgagttcg aaattctac 
      ttggacttt cacgcttct agaaaaaca accgatctt 
      actcaaacg ccttggacg cataatccg agcgataac 
      agcaagacc cgtgttgtt aacttaacg gtagcttta 
      acacaggcc atggggccg aaaacggct caagttacg 
      gagactcaa gtcatgctt ccatgtagc aaagctggg 
      tgtctttac gccatagac gtggacaca gtaaacgcc 
      ggcattcct tacgctgat agctttaat attgtagtt 
      catcactgt cttcaaaaa atttccgag acggaaagc 
      tcgtatcag gtgtttgca caagtcatg tacaaaaaa 
      tcggtctgg ggcttggtc aagggcatg atcgagaag 
      acgtgttgg tcgggcttg gaggacttt tacgtttct 
      ctagccaaa gctttgcac gtcgaaggc gaggaaaac 
      gtctgcgac gtgaagcgc aagtccagg aggaaacgc 
      aggattcac agcatgccg aggccgggg gttgaggac 
      tccaggcct ctgataggg gcctccagc cggatttcc 
      tccgggggc attttcagc tcggacgtc gccacgatg 
      atcgtcttc actgttcta atcctgtta gttgttctc 
      aatgtcatg ttgtacttc aaactgtgg tctttagaa 
      gagtcgcct ccgtacacc ctcttggac ttgcatgtc 
      ttgaaagac ccacccaag acacacgat gaatggatt 
      aaactactc caacaacag gaaacgctg cattcggtc 
      gagttgcaa aagtggcag cgggttttg aaaaccgcg 
      atacaactt ttaaaacag accgaggag tcgctgaac 
      gaactgcaa cggtcgatt aatcccagt tattcaagc 
      aaggtcatg tcgattctg cagaatcac aaggataca 
      gtcgagcag aatgagaag agcgaactt tag
    • >TC000335 RB
      atgaagatg acattatcg tcgacaaat ggtacaacg 
      aacgaaaag ttaaccaca ccgacagaa aacaagcaa 
      ggcagcccc aattctagc cctcgggct agccccaga 
      ccgtccccg aggccgcag ccgaaacgg gaacacccc 
      aaaagtgag acgcacctc accgtcaat tacaaggaa 
      atccccgga agtaacaag cacgatggg tccccttct 
      agtcaggag agttctgta agtagtcgc gccagtaat 
      ttggacatt agcccgccc tcgaacttg aaagttgag 
      agcaccagt agctcgaat aaagaacgt aaggaccca 
      cgtggtaag aagaagtcg tcttggttc aatcccttc 
      tacccgacc tacaagtca cgatcggag gattttaag 
      agaattttt aaggacgta cctgacgat gagcgtttg 
      ctagtcgat tattcctgc gctttgcaa aaagaaata 
      ttggcacaa ggcagactc tacgtcacg caaaactat 
      ctctgcttt tacgcgaat attctggga tgggagacc 
      acacttaag ctaaaatgg aaggacgta tcagccata 
      accaaagag aaaactgca atcgtaatc cccaacgcg 
      gttttgatt tgcacaagg acggaaaag tactttttc 
      accagtttt gtagcacgg gacaagacg tatttgatg 
      ctgtttcga gtatggcag aacgcgctc atggaccaa 
      cctatgtct ccacaggag atgtggcaa tgggtgcac 
      cagtggtat ggtgaagag ctgggcttg accagtgat 
      gatgaggac tacgtagcc cccggtact gaagaggac 
      aaactatcg gcacgctta tctgtcgaa tctttctct 
      gagcacgag agcagcgct gttgagaac ccactggaa 
      gcgatggtg gtggaggag aagccccca caagacgac 
      gacagcgtc accaatagg ccggtggat cgccgccga 
      actacttct tcgcatctc cctactgat tactccgat 
      acgaccgaa tcggacgga gaaaaacaa acgaaatgt 
      gatacacac gtacagtgc acttcgact cacgaaggt 
      cgtcaaata atgaacgaa atcgtacct atccatata 
      gatcaatta tttactcta ctttttacg agttcgaaa 
      ttctacttg gactttcac gcttctaga aaaacaacc 
      gatcttact caaacgcct tggacgcat aatccgagc 
      gataacagc aagacccgt gttgttaac ttaacggta 
      gctttaaca caggccatg gggccgaaa acggctcaa 
      gttacggag actcaagtc atgcttcca tgtagcaaa 
      gctgggtgt ctttacgcc atagacgtg gacacagta 
      aacgccggc attccttac gctgatagc tttaatatt 
      gtagttcat cactgtctt caaaaaatt tccgagacg 
      gaaagctcg tatcaggtg tttgcacaa gtcatgtac 
      aaaaaatcg gtctggggc ttggtcaag ggcatgatc 
      gagaagacg tgttggtcg ggcttggag gacttttac 
      gtttctcta gccaaagct ttgcacgtc gaaggcgag 
      gaaaacgtc tgcgacgtg aagcgcaag tccaggagg 
      aaacgcagg attcacagc atgccgagg ccgggggtt 
      gaggactcc aggcctctg ataggggcc tccagccgg 
      atttcctcc gggggcatt ttcagctcg gacgtcgcc 
      acgatgatc gtcttcact gttctaatc ctgttagtt 
      gttctcaat gtcatgttg tacttcaaa ctgtggtct 
      ttagaagag tcgcctccg tacaccctc ttggacttg 
      catgtcttg aaagaccca cccaagaca cacgatgaa 
      tggattaaa ctactccaa caacaggaa acgctgcat 
      tcggtcgag ttgcaaaag tggcagcgg gttttgaaa 
      accgcgata caactttta aaacagacc gaggagtcg 
      ctgaacgaa ctgcaacgg tcgattaat cccagttat 
      tcaagcaag gtcatgtcg attctgcag aatcacaag 
      gatacagtc gagcagaat gagaagagc gaactttag
    mRNA sequence
    • >TC000335 RA
      gtagtagcc acctagcat tacgtttgt atttgagca 
      agttctttt ttaattttt ctaccattc gtttttgca 
      atggagtgg tggtcgttg atgggtctg tgggcagga 
      aagccagtg attttcagt aatccctag tgagtgtct 
      gcatttaga accaccaaa aagtgtttt attagctta 
      atcaaccgt gaactggcc attaacacg taaagaaac 
      caagataac caaacagta attaatgca atgtgataa 
      cagtgacga ttctcctcg tttccgttg aatttcaag 
      caaaaacag cttcatgag gggccacag gaagagcgc 
      ctctttctc aaaatgaag atgacatta tcgtcgaca 
      aatggtaca acgaacgaa aagttaacc acaccgaca 
      gaaaacaag caaggcagc cccaattct agccctcgg 
      gctagcccc agaccgtcc ccgaggccg cagccgaaa 
      cgggaacac cccaaaagt gagacgcac ctcaccgtc 
      aattacaag gaaatcccc ggaagtaac aagcacgat 
      gggtcccct tctagtcag gagagttct gtaagtagt 
      cgcgccagt aatttggac attagcccg ccctcgaac 
      ttgaaagtt gagagcacc agtagctcg aataaagaa 
      cgtaaggac ccacgtggt aagaagaag tcgtcttgg 
      ttcaatccc ttctacccg acctacaag tcacgatcg 
      gaggatttt aagagaatt tttaaggac gtacctgac 
      gatgagcgt ttgctagtc gattattcc tgcgctttg 
      caaaaagaa atattggca caaggcaga ctctacgtc 
      acgcaaaac tatctctgc ttttacgcg aatattctg 
      ggatgggag accacactt aagctaaaa tggaaggac 
      gtatcagcc ataaccaaa gagaaaact gcaatcgta 
      atccccaac gcggttttg atttgcaca aggacggaa 
      aagtacttt ttcaccagt tttgtagca cgggacaag 
      acgtatttg atgctgttt cgagtatgg cagaacgcg 
      ctcatggac caacctatg tctccacag gagatgtgg 
      caatgggtg caccagtgg tatggtgaa gagctgggc 
      ttgaccagt gatgatgag gactacgta gcccccggt 
      actgaagag gacaaacta tcggcacgc ttatctgtc 
      gaatctttc tctgagcac gagagcagc gctgttgag 
      aacccactg gaagcgatg gtggtggag gagaagccc 
      ccacaagac gacgacagc gtcaccaat aggccggtg 
      gatcgccgc cgaactact tcttcgcat ctccctact 
      gattactcc gatacgacc gaatcggac ggagaaaaa 
      caaacgaaa actaaaccg acctttttc ctatcctgg 
      gataaatcc tctcctttt ttacaccct tcaagtccc 
      gaatatgac gatactgtt ccggttttg ccgaatgtc 
      aaatgtgat acacacgta cagtgcact tcgactcac 
      gaaggtcgt caaataatg aacgaaatc gtacctatc 
      catatagat caattattt actctactt tttacgagt 
      tcgaaattc tacttggac tttcacgct tctagaaaa 
      acaaccgat cttactcaa acgccttgg acgcataat 
      ccgagcgat aacagcaag acccgtgtt gttaactta 
      acggtagct ttaacacag gccatgggg ccgaaaacg 
      gctcaagtt acggagact caagtcatg cttccatgt 
      agcaaagct gggtgtctt tacgccata gacgtggac 
      acagtaaac gccggcatt ccttacgct gatagcttt 
      aatattgta gttcatcac tgtcttcaa aaaatttcc 
      gagacggaa agctcgtat caggtgttt gcacaagtc 
      atgtacaaa aaatcggtc tggggcttg gtcaagggc 
      atgatcgag aagacgtgt tggtcgggc ttggaggac 
      ttttacgtt tctctagcc aaagctttg cacgtcgaa 
      ggcgaggaa aacgtctgc gacgtgaag cgcaagtcc 
      aggaggaaa cgcaggatt cacagcatg ccgaggccg 
      ggggttgag gactccagg cctctgata ggggcctcc 
      agccggatt tcctccggg ggcattttc agctcggac 
      gtcgccacg atgatcgtc ttcactgtt ctaatcctg 
      ttagttgtt ctcaatgtc atgttgtac ttcaaactg 
      tggtcttta gaagagtcg cctccgtac accctcttg 
      gacttgcat gtcttgaaa gacccaccc aagacacac 
      gatgaatgg attaaacta ctccaacaa caggaaacg 
      ctgcattcg gtcgagttg caaaagtgg cagcgggtt 
      ttgaaaacc gcgatacaa cttttaaaa cagaccgag 
      gagtcgctg aacgaactg caacggtcg attaatccc 
      agttattca agcaaggtc atgtcgatt ctgcagaat 
      cacaaggat acagtcgag cagaatgag aagagcgaa 
      ctttaggat tgttatgtt tttgaaatt tattctacg 
      tcactacgc actgtgtta gatttttag tacctttag 
      agattttat cgattgttc ctgtacacg ttgtgtaga 
      tatttaaat aaattacaa caacctaa
    • >TC000335 RB
      gtagtagcc acctagcat tacgtttgt atttgagca 
      agttctttt ttaattttt ctaccattc gtttttgca 
      atggagtgg tggtcgttg atgggtctg tgggcagga 
      aagccagtg attttcagt aatccctag tgagtgtct 
      gcatttaga accaccaaa aagtgtttt attagctta 
      atcaaccgt gaactggcc attaacacg taaagaaac 
      caagataac caaacagta attaatgca atgtgataa 
      cagtgacga ttctcctcg tttccgttg aatttcaag 
      caaaaacag cttcatgag gggccacag gaagagcgc 
      ctctttctc aaaatgaag atgacatta tcgtcgaca 
      aatggtaca acgaacgaa aagttaacc acaccgaca 
      gaaaacaag caaggcagc cccaattct agccctcgg 
      gctagcccc agaccgtcc ccgaggccg cagccgaaa 
      cgggaacac cccaaaagt gagacgcac ctcaccgtc 
      aattacaag gaaatcccc ggaagtaac aagcacgat 
      gggtcccct tctagtcag gagagttct gtaagtagt 
      cgcgccagt aatttggac attagcccg ccctcgaac 
      ttgaaagtt gagagcacc agtagctcg aataaagaa 
      cgtaaggac ccacgtggt aagaagaag tcgtcttgg 
      ttcaatccc ttctacccg acctacaag tcacgatcg 
      gaggatttt aagagaatt tttaaggac gtacctgac 
      gatgagcgt ttgctagtc gattattcc tgcgctttg 
      caaaaagaa atattggca caaggcaga ctctacgtc 
      acgcaaaac tatctctgc ttttacgcg aatattctg 
      ggatgggag accacactt aagctaaaa tggaaggac 
      gtatcagcc ataaccaaa gagaaaact gcaatcgta 
      atccccaac gcggttttg atttgcaca aggacggaa 
      aagtacttt ttcaccagt tttgtagca cgggacaag 
      acgtatttg atgctgttt cgagtatgg cagaacgcg 
      ctcatggac caacctatg tctccacag gagatgtgg 
      caatgggtg caccagtgg tatggtgaa gagctgggc 
      ttgaccagt gatgatgag gactacgta gcccccggt 
      actgaagag gacaaacta tcggcacgc ttatctgtc 
      gaatctttc tctgagcac gagagcagc gctgttgag 
      aacccactg gaagcgatg gtggtggag gagaagccc 
      ccacaagac gacgacagc gtcaccaat aggccggtg 
      gatcgccgc cgaactact tcttcgcat ctccctact 
      gattactcc gatacgacc gaatcggac ggagaaaaa 
      caaacgaaa tgtgataca cacgtacag tgcacttcg 
      actcacgaa ggtcgtcaa ataatgaac gaaatcgta 
      cctatccat atagatcaa ttatttact ctacttttt 
      acgagttcg aaattctac ttggacttt cacgcttct 
      agaaaaaca accgatctt actcaaacg ccttggacg 
      cataatccg agcgataac agcaagacc cgtgttgtt 
      aacttaacg gtagcttta acacaggcc atggggccg 
      aaaacggct caagttacg gagactcaa gtcatgctt 
      ccatgtagc aaagctggg tgtctttac gccatagac 
      gtggacaca gtaaacgcc ggcattcct tacgctgat 
      agctttaat attgtagtt catcactgt cttcaaaaa 
      atttccgag acggaaagc tcgtatcag gtgtttgca 
      caagtcatg tacaaaaaa tcggtctgg ggcttggtc 
      aagggcatg atcgagaag acgtgttgg tcgggcttg 
      gaggacttt tacgtttct ctagccaaa gctttgcac 
      gtcgaaggc gaggaaaac gtctgcgac gtgaagcgc 
      aagtccagg aggaaacgc aggattcac agcatgccg 
      aggccgggg gttgaggac tccaggcct ctgataggg 
      gcctccagc cggatttcc tccgggggc attttcagc 
      tcggacgtc gccacgatg atcgtcttc actgttcta 
      atcctgtta gttgttctc aatgtcatg ttgtacttc 
      aaactgtgg tctttagaa gagtcgcct ccgtacacc 
      ctcttggac ttgcatgtc ttgaaagac ccacccaag 
      acacacgat gaatggatt aaactactc caacaacag 
      gaaacgctg cattcggtc gagttgcaa aagtggcag 
      cgggttttg aaaaccgcg atacaactt ttaaaacag 
      accgaggag tcgctgaac gaactgcaa cggtcgatt 
      aatcccagt tattcaagc aaggtcatg tcgattctg 
      cagaatcac aaggataca gtcgagcag aatgagaag 
      agcgaactt taggattgt tatgttttt gaaatttat 
      tctacgtca ctacgcact gtgttagat ttttagtac 
      ctttagaga ttttatcga ttgttcctg tacacgttg 
      tgtagatat ttaaataaa ttacaacaa cctaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY