General information | |||
TC000331 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 12998089..13001227 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC000331 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00074 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 62.5% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
- >TC000331 RA
MADWEEIKR LAADFQKVQ LSSTSQKLS ERNCIEIIN YLKEKKIID LIFTVDGKE FLTPSHLIQ DIKNELFVS GGRINLVEL AKNIGVDLA HITAHLKDV LKGQKDLHS ILGQLIDSS YITKIAGEI NEKLAQQGQ ISISDLTIQ YDLSAEFLQ QQVVEKHLG KIIHGKQDT NDPRVIFTE SFIARCKAK IRGALCGLT RPTPVASIL NQISLSEKL FFSLFDQTS MHGALTSRV VGAQYIPNV YARSQNEWV NNFYKQNDY LEYDALARL GISDYKSFI KKQLSSEDL VFLNSCAAS KRVLERAEA DIDECISSK SYLDLQSNL PSVFNDKDI RLILDTVLT KQKQQQTIV IENFILSKA FIENLSKNF DELVKEKAK AVVESGKYQ QYQTDIQIS SSKPQKFEE VDEKVDKRE ERRKKAAGG KSGGGTQGR ETKTKSTKK AKKHVEEEF ETVERRQQL QVVLSEDIA DLIQKELEE DGLDELSEP LTQYLLPQL NEKGLEIAG EIYAATIAD RTANRRQTH NELQNKLNA LIGDVRLFE KGLKLLPEE LQGQLVKYL LKTLCTDIT TEILNYVAA EQNLNTQTD NFTNEQRLK FVNELPPDL KKSLLPLIK SLSGQSIDE FMAAVEESL SVCSMIIKK IDKKKDRTI VLNHKHVLL EQLNKCDDL ALVLHLTTL IIFTTATQC MLHASGRHV AAILSFLKQ YLTDGQVAE LTSYHDFVT LMLSGGSEA ESAKEKLKE KIQTVKNIA NEFKKPGAE KS
- >TC000331 RA
atggcagat tgggaagaa atcaagcgt ttggcggcg gattttcaa aaagtgcaa ctgagttcc acgtcacaa aaactgtct gagcgcaac tgcatagaa ataatcaac tacttgaaa gaaaagaaa ataatcgat ttaattttc accgtcgac ggtaaagaa ttcctgacc ccttcccac ctcatccaa gacatcaaa aacgagctg ttcgtcagc ggcggccgc ataaatcta gtcgaacta gcaaaaaac atcggcgtt gacttggcc cacataaca gcccacctt aaagacgtc ctcaagggc caaaaggac ctccactcc atcctaggc caactgatc gactcctcg tacatcact aaaatcgca ggcgaaatt aacgagaag ctcgcacaa caaggccaa atcagcatc agtgatcta acaattcag tatgacctc agtgccgaa tttctccaa cagcaagtt gtggagaaa catttgggc aaaatcatt cacggcaaa caggacacc aacgacccc agagtcatt ttcaccgaa tcgttcatc gccagatgc aaagccaag atcaggggg gctctgtgt ggcttgacc agacctacg ccggttgcc tcaatcctg aaccaaatt agtttgagc gaaaagctg tttttctcg ctttttgac cagacttcc atgcacggc gccctcacc agcagggtc gtgggggcc cagtacatc cccaacgtc tacgccagg tcacagaac gaatgggtc aacaacttt tataagcaa aacgattat ctggagtat gatgcgttg gcaaggctc ggaatctcg gattacaag tcgttcatc aaaaagcaa ctgtcgtct gaagacttg gtttttctc aattcttgc gctgcttcc aaacgtgtg ttagagcgg gcggaggct gatattgat gaatgtatc tctagcaag tcttatctc gacttacaa tcgaacctc ccgtccgtt tttaatgac aaagatatt agactaatc ctcgataca gttttgacg aaacaaaag caacaacaa actatagta atcgagaat tttattttg agcaaagct tttatcgaa aatttgtcg aaaaacttc gacgaactc gtaaaagag aaggctaaa gccgttgtg gagtctggt aaataccag cagtaccag accgatatt caaatttcg tcaagtaag ccgcagaag tttgaagaa gtggatgag aaggttgat aagagggaa gaaaggcgg aagaaggcg gctgggggt aaaagcggg ggtgggacg caggggcgc gagactaag accaagtct accaagaag gccaagaag cacgtggag gaggagttc gagacggtt gagcggaga caacagctg caggtcgtg ttgagtgag gatattgca gatttgatt cagaaggag ctggaagag gacggtttg gacgaatta agtgagcct ctaactcag tatttgttg ccacaattg aatgagaaa ggattggag attgcggga gaaatttac gcggcgact atcgccgac aggacggca aaccgcaga caaacgcat aacgagctc caaaataaa ttgaacgcg ctaatcgga gacgtgcga ctcttcgag aaaggtctg aaactgctg cccgaggaa ttgcaaggc cagttggtc aaatatctc ctcaagacg ctctgcacc gatattacc acggaaatc ttgaattac gtggccgct gaacaaaat ctcaacact caaactgat aatttcacc aatgagcag cgattaaag ttcgttaac gagttacca cccgacttg aaaaagtca cttttgccc ctcattaag tcgctaagc gggcaaagt atcgacgag ttcatggct gccgtggag gaaagtctc agcgtatgc agtatgatt atcaagaaa atcgataaa aagaaagac aggacgata gttttgaac cataagcat gttttgtta gaacagttg aataaatgc gacgatttg gcgcttgtt ttgcatttg acgacttta attattttc acgacggcc actcaatgc atgctccat gccagtggt agacacgtg gcagcgatt ttaagtttt ttgaaacaa tatttaacc gacggacaa gtcgccgaa ttgacgtcc tatcacgat tttgtcaca ttgatgcta agtgggggg agcgaagcc gaaagtgcg aaagaaaaa ctcaaagag aagatacaa acggtcaaa aatatcgcc aatgagttc aaaaaaccc ggcgctgag aagagttaa
- >TC000331 RA
tatcggcaa aatatgcgt cacgtggta ctaattacg tcatatgtc aagttgtga tgtgacaac agtaagcaa attgttttg cttgttaaa ccatatggt ccagtatta aaattgtaa taattttac attaacaaa ccatatttc gtacaaatt catttcgcg ccgacctta cattgtatt aattgttga gtgtttcct tcaaaactg aaaaatcat cgtgaggat ggcagattg ggaagaaat caagcgttt ggcggcgga ttttcaaaa agtgcaact gagttccac gtcacaaaa actgtctga gcgcaactg catagaaat aatcaacta cttgaaaga aaagaaaat aatcgattt aattttcac cgtcgacgg taaagaatt cctgacccc ttcccacct catccaaga catcaaaaa cgagctgtt cgtcagcgg cggccgcat aaatctagt cgaactagc aaaaaacat cggcgttga cttggccca cataacagc ccaccttaa agacgtcct caagggcca aaaggacct ccactccat cctaggcca actgatcga ctcctcgta catcactaa aatcgcagg cgaaattaa cgagaagct cgcacaaca aggccaaat cagcatcag tgatctaac aattcagta tgacctcag tgccgaatt tctccaaca gcaagttgt ggagaaaca tttgggcaa aatcattca cggcaaaca ggacaccaa cgaccccag agtcatttt caccgaatc gttcatcgc cagatgcaa agccaagat caggggggc tctgtgtgg cttgaccag acctacgcc ggttgcctc aatcctgaa ccaaattag tttgagcga aaagctgtt tttctcgct ttttgacca gacttccat gcacggcgc cctcaccag cagggtcgt gggggccca gtacatccc caacgtcta cgccaggtc acagaacga atgggtcaa caactttta taagcaaaa cgattatct ggagtatga tgcgttggc aaggctcgg aatctcgga ttacaagtc gttcatcaa aaagcaact gtcgtctga agacttggt ttttctcaa ttcttgcgc tgcttccaa acgtgtgtt agagcgggc ggaggctga tattgatga atgtatctc tagcaagtc ttatctcga cttacaatc gaacctccc gtccgtttt taatgacaa agatattag actaatcct cgatacagt tttgacgaa acaaaagca acaacaaac tatagtaat cgagaattt tattttgag caaagcttt tatcgaaaa tttgtcgaa aaacttcga cgaactcgt aaaagagaa ggctaaagc cgttgtgga gtctggtaa ataccagca gtaccagac cgatattca aatttcgtc aagtaagcc gcagaagtt tgaagaagt ggatgagaa ggttgataa gagggaaga aaggcggaa gaaggcggc tgggggtaa aagcggggg tgggacgca ggggcgcga gactaagac caagtctac caagaaggc caagaagca cgtggagga ggagttcga gacggttga gcggagaca acagctgca ggtcgtgtt gagtgagga tattgcaga tttgattca gaaggagct ggaagagga cggtttgga cgaattaag tgagcctct aactcagta tttgttgcc acaattgaa tgagaaagg attggagat tgcgggaga aatttacgc ggcgactat cgccgacag gacggcaaa ccgcagaca aacgcataa cgagctcca aaataaatt gaacgcgct aatcggaga cgtgcgact cttcgagaa aggtctgaa actgctgcc cgaggaatt gcaaggcca gttggtcaa atatctcct caagacgct ctgcaccga tattaccac ggaaatctt gaattacgt ggccgctga acaaaatct caacactca aactgataa tttcaccaa tgagcagcg attaaagtt cgttaacga gttaccacc cgacttgaa aaagtcact tttgcccct cattaagtc gctaagcgg gcaaagtat cgacgagtt catggctgc cgtggagga aagtctcag cgtatgcag tatgattat caagaaaat cgataaaaa gaaagacag gacgatagt tttgaacca taagcatgt tttgttaga acagttgaa taaatgcga cgatttggc gcttgtttt gcatttgac gactttaat tattttcac gacggccac tcaatgcat gctccatgc cagtggtag acacgtggc agcgatttt aagtttttt gaaacaata tttaaccga cggacaagt cgccgaatt gacgtccta tcacgattt tgtcacatt gatgctaag tggggggag cgaagccga aagtgcgaa agaaaaact caaagagaa gatacaaac ggtcaaaaa tatcgccaa tgagttcaa aaaacccgg cgctgagaa gagttaatg cttgcgaaa acatgtaca taagttact tgtaatata tgcaaataa acgtttatt taaaat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
