Status
Please wait ...
Gene information
General information
TC000331 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12998089..13001227
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000331
    iBeetle screen iB_00074

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 62.5% (includes death as larva, prepupa, pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    Pupal morphology
    antenna size shortened - Number of animals showing the phenotype: 2

    elytra size shortened - Number of animals showing the phenotype: 4

    Adult morphology
    wings not closed - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000331 RA
      MADWEEIKR LAADFQKVQ LSSTSQKLS ERNCIEIIN 
      YLKEKKIID LIFTVDGKE FLTPSHLIQ DIKNELFVS 
      GGRINLVEL AKNIGVDLA HITAHLKDV LKGQKDLHS 
      ILGQLIDSS YITKIAGEI NEKLAQQGQ ISISDLTIQ 
      YDLSAEFLQ QQVVEKHLG KIIHGKQDT NDPRVIFTE 
      SFIARCKAK IRGALCGLT RPTPVASIL NQISLSEKL 
      FFSLFDQTS MHGALTSRV VGAQYIPNV YARSQNEWV 
      NNFYKQNDY LEYDALARL GISDYKSFI KKQLSSEDL 
      VFLNSCAAS KRVLERAEA DIDECISSK SYLDLQSNL 
      PSVFNDKDI RLILDTVLT KQKQQQTIV IENFILSKA 
      FIENLSKNF DELVKEKAK AVVESGKYQ QYQTDIQIS 
      SSKPQKFEE VDEKVDKRE ERRKKAAGG KSGGGTQGR 
      ETKTKSTKK AKKHVEEEF ETVERRQQL QVVLSEDIA 
      DLIQKELEE DGLDELSEP LTQYLLPQL NEKGLEIAG 
      EIYAATIAD RTANRRQTH NELQNKLNA LIGDVRLFE 
      KGLKLLPEE LQGQLVKYL LKTLCTDIT TEILNYVAA 
      EQNLNTQTD NFTNEQRLK FVNELPPDL KKSLLPLIK 
      SLSGQSIDE FMAAVEESL SVCSMIIKK IDKKKDRTI 
      VLNHKHVLL EQLNKCDDL ALVLHLTTL IIFTTATQC 
      MLHASGRHV AAILSFLKQ YLTDGQVAE LTSYHDFVT 
      LMLSGGSEA ESAKEKLKE KIQTVKNIA NEFKKPGAE 
      KS
    CDS sequence
    • >TC000331 RA
      atggcagat tgggaagaa atcaagcgt ttggcggcg 
      gattttcaa aaagtgcaa ctgagttcc acgtcacaa 
      aaactgtct gagcgcaac tgcatagaa ataatcaac 
      tacttgaaa gaaaagaaa ataatcgat ttaattttc 
      accgtcgac ggtaaagaa ttcctgacc ccttcccac 
      ctcatccaa gacatcaaa aacgagctg ttcgtcagc 
      ggcggccgc ataaatcta gtcgaacta gcaaaaaac 
      atcggcgtt gacttggcc cacataaca gcccacctt 
      aaagacgtc ctcaagggc caaaaggac ctccactcc 
      atcctaggc caactgatc gactcctcg tacatcact 
      aaaatcgca ggcgaaatt aacgagaag ctcgcacaa 
      caaggccaa atcagcatc agtgatcta acaattcag 
      tatgacctc agtgccgaa tttctccaa cagcaagtt 
      gtggagaaa catttgggc aaaatcatt cacggcaaa 
      caggacacc aacgacccc agagtcatt ttcaccgaa 
      tcgttcatc gccagatgc aaagccaag atcaggggg 
      gctctgtgt ggcttgacc agacctacg ccggttgcc 
      tcaatcctg aaccaaatt agtttgagc gaaaagctg 
      tttttctcg ctttttgac cagacttcc atgcacggc 
      gccctcacc agcagggtc gtgggggcc cagtacatc 
      cccaacgtc tacgccagg tcacagaac gaatgggtc 
      aacaacttt tataagcaa aacgattat ctggagtat 
      gatgcgttg gcaaggctc ggaatctcg gattacaag 
      tcgttcatc aaaaagcaa ctgtcgtct gaagacttg 
      gtttttctc aattcttgc gctgcttcc aaacgtgtg 
      ttagagcgg gcggaggct gatattgat gaatgtatc 
      tctagcaag tcttatctc gacttacaa tcgaacctc 
      ccgtccgtt tttaatgac aaagatatt agactaatc 
      ctcgataca gttttgacg aaacaaaag caacaacaa 
      actatagta atcgagaat tttattttg agcaaagct 
      tttatcgaa aatttgtcg aaaaacttc gacgaactc 
      gtaaaagag aaggctaaa gccgttgtg gagtctggt 
      aaataccag cagtaccag accgatatt caaatttcg 
      tcaagtaag ccgcagaag tttgaagaa gtggatgag 
      aaggttgat aagagggaa gaaaggcgg aagaaggcg 
      gctgggggt aaaagcggg ggtgggacg caggggcgc 
      gagactaag accaagtct accaagaag gccaagaag 
      cacgtggag gaggagttc gagacggtt gagcggaga 
      caacagctg caggtcgtg ttgagtgag gatattgca 
      gatttgatt cagaaggag ctggaagag gacggtttg 
      gacgaatta agtgagcct ctaactcag tatttgttg 
      ccacaattg aatgagaaa ggattggag attgcggga 
      gaaatttac gcggcgact atcgccgac aggacggca 
      aaccgcaga caaacgcat aacgagctc caaaataaa 
      ttgaacgcg ctaatcgga gacgtgcga ctcttcgag 
      aaaggtctg aaactgctg cccgaggaa ttgcaaggc 
      cagttggtc aaatatctc ctcaagacg ctctgcacc 
      gatattacc acggaaatc ttgaattac gtggccgct 
      gaacaaaat ctcaacact caaactgat aatttcacc 
      aatgagcag cgattaaag ttcgttaac gagttacca 
      cccgacttg aaaaagtca cttttgccc ctcattaag 
      tcgctaagc gggcaaagt atcgacgag ttcatggct 
      gccgtggag gaaagtctc agcgtatgc agtatgatt 
      atcaagaaa atcgataaa aagaaagac aggacgata 
      gttttgaac cataagcat gttttgtta gaacagttg 
      aataaatgc gacgatttg gcgcttgtt ttgcatttg 
      acgacttta attattttc acgacggcc actcaatgc 
      atgctccat gccagtggt agacacgtg gcagcgatt 
      ttaagtttt ttgaaacaa tatttaacc gacggacaa 
      gtcgccgaa ttgacgtcc tatcacgat tttgtcaca 
      ttgatgcta agtgggggg agcgaagcc gaaagtgcg 
      aaagaaaaa ctcaaagag aagatacaa acggtcaaa 
      aatatcgcc aatgagttc aaaaaaccc ggcgctgag 
      aagagttaa
    mRNA sequence
    • >TC000331 RA
      tatcggcaa aatatgcgt cacgtggta ctaattacg 
      tcatatgtc aagttgtga tgtgacaac agtaagcaa 
      attgttttg cttgttaaa ccatatggt ccagtatta 
      aaattgtaa taattttac attaacaaa ccatatttc 
      gtacaaatt catttcgcg ccgacctta cattgtatt 
      aattgttga gtgtttcct tcaaaactg aaaaatcat 
      cgtgaggat ggcagattg ggaagaaat caagcgttt 
      ggcggcgga ttttcaaaa agtgcaact gagttccac 
      gtcacaaaa actgtctga gcgcaactg catagaaat 
      aatcaacta cttgaaaga aaagaaaat aatcgattt 
      aattttcac cgtcgacgg taaagaatt cctgacccc 
      ttcccacct catccaaga catcaaaaa cgagctgtt 
      cgtcagcgg cggccgcat aaatctagt cgaactagc 
      aaaaaacat cggcgttga cttggccca cataacagc 
      ccaccttaa agacgtcct caagggcca aaaggacct 
      ccactccat cctaggcca actgatcga ctcctcgta 
      catcactaa aatcgcagg cgaaattaa cgagaagct 
      cgcacaaca aggccaaat cagcatcag tgatctaac 
      aattcagta tgacctcag tgccgaatt tctccaaca 
      gcaagttgt ggagaaaca tttgggcaa aatcattca 
      cggcaaaca ggacaccaa cgaccccag agtcatttt 
      caccgaatc gttcatcgc cagatgcaa agccaagat 
      caggggggc tctgtgtgg cttgaccag acctacgcc 
      ggttgcctc aatcctgaa ccaaattag tttgagcga 
      aaagctgtt tttctcgct ttttgacca gacttccat 
      gcacggcgc cctcaccag cagggtcgt gggggccca 
      gtacatccc caacgtcta cgccaggtc acagaacga 
      atgggtcaa caactttta taagcaaaa cgattatct 
      ggagtatga tgcgttggc aaggctcgg aatctcgga 
      ttacaagtc gttcatcaa aaagcaact gtcgtctga 
      agacttggt ttttctcaa ttcttgcgc tgcttccaa 
      acgtgtgtt agagcgggc ggaggctga tattgatga 
      atgtatctc tagcaagtc ttatctcga cttacaatc 
      gaacctccc gtccgtttt taatgacaa agatattag 
      actaatcct cgatacagt tttgacgaa acaaaagca 
      acaacaaac tatagtaat cgagaattt tattttgag 
      caaagcttt tatcgaaaa tttgtcgaa aaacttcga 
      cgaactcgt aaaagagaa ggctaaagc cgttgtgga 
      gtctggtaa ataccagca gtaccagac cgatattca 
      aatttcgtc aagtaagcc gcagaagtt tgaagaagt 
      ggatgagaa ggttgataa gagggaaga aaggcggaa 
      gaaggcggc tgggggtaa aagcggggg tgggacgca 
      ggggcgcga gactaagac caagtctac caagaaggc 
      caagaagca cgtggagga ggagttcga gacggttga 
      gcggagaca acagctgca ggtcgtgtt gagtgagga 
      tattgcaga tttgattca gaaggagct ggaagagga 
      cggtttgga cgaattaag tgagcctct aactcagta 
      tttgttgcc acaattgaa tgagaaagg attggagat 
      tgcgggaga aatttacgc ggcgactat cgccgacag 
      gacggcaaa ccgcagaca aacgcataa cgagctcca 
      aaataaatt gaacgcgct aatcggaga cgtgcgact 
      cttcgagaa aggtctgaa actgctgcc cgaggaatt 
      gcaaggcca gttggtcaa atatctcct caagacgct 
      ctgcaccga tattaccac ggaaatctt gaattacgt 
      ggccgctga acaaaatct caacactca aactgataa 
      tttcaccaa tgagcagcg attaaagtt cgttaacga 
      gttaccacc cgacttgaa aaagtcact tttgcccct 
      cattaagtc gctaagcgg gcaaagtat cgacgagtt 
      catggctgc cgtggagga aagtctcag cgtatgcag 
      tatgattat caagaaaat cgataaaaa gaaagacag 
      gacgatagt tttgaacca taagcatgt tttgttaga 
      acagttgaa taaatgcga cgatttggc gcttgtttt 
      gcatttgac gactttaat tattttcac gacggccac 
      tcaatgcat gctccatgc cagtggtag acacgtggc 
      agcgatttt aagtttttt gaaacaata tttaaccga 
      cggacaagt cgccgaatt gacgtccta tcacgattt 
      tgtcacatt gatgctaag tggggggag cgaagccga 
      aagtgcgaa agaaaaact caaagagaa gatacaaac 
      ggtcaaaaa tatcgccaa tgagttcaa aaaacccgg 
      cgctgagaa gagttaatg cttgcgaaa acatgtaca 
      taagttact tgtaatata tgcaaataa acgtttatt 
      taaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY