Status
Please wait ...
Gene information
General information
TC000326 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 7515348..7519045
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000326
    iBeetle screen iB_00073
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    pupation - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    remarks: later than schedule, hours 24
    Pupal morphology
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000326 RA
      MVEGQPLHY VYQPDYFYP YKMEINDDL IVPSSPNST 
      QSDSDNSLS SIEVSPKPI RARSVIQHT PKNEVRCGW 
      KSCGITFDS LDQLATHVT KIHASSGPG GLFYCGWEG 
      CSRNSKGFN ARYKMLVHV RTHTNEKPH QCCQCEKSF 
      SRAENLKIH SRSHSGEKP YVCPVPGCN KAYSNSSDR 
      FKHTRTHQV EKPYQCKVP GCPKRYTDP SSLRKHVKT 
      YKHFVDKVD ENAKSNSIE KVQEGHSCD CTHMCCAGK 
      DKARFGGLI GYSRAESPY FFAPGTEMD VDVPLDLSI 
      NRKV
    CDS sequence
    • >TC000326 RA
      atggtcgag ggccaaccc ctccactac gtctaccaa 
      cccgactac ttctacccc tacaaaatg gaaattaac 
      gacgaccta atcgtcccc agctcgccc aacagcaca 
      cagagtgac agtgacaac tccctatcc agcatcgaa 
      gtctccccg aaaccgatc cgcgccagg agcgtcatc 
      caacacacc cccaaaaac gaggtccgc tgcggctgg 
      aagtcctgc gggatcacg ttcgattcc ctggaccaa 
      ctcgccacg cacgtgacg aaaatccac gcgtccagc 
      ggtcccggg ggcctcttc tactgcggc tgggagggc 
      tgttcccgc aacagcaag ggttttaac gccaggtat 
      aagatgttg gtgcacgtg cggacgcac accaacgag 
      aaaccgcac cagtgctgc caatgcgag aaatccttc 
      tcgcgcgcc gaaaatttg aaaatccac tcgcggtcg 
      cacagcggg gaaaaaccg tacgtgtgt ccggtcccg 
      gggtgcaac aaggcctac tcgaattcc agcgaccgg 
      ttcaagcac acgcggacg caccaggtg gagaagccg 
      taccagtgc aaggtgcca gggtgcccc aagcgctac 
      accgacccc agcagtctc cggaagcac gtcaagacg 
      tacaagcac ttcgtcgat aaggtggac gagaacgcc 
      aaaagtaac agtattgag aaggtgcaa gaggggcat 
      tcgtgcgat tgcacgcac atgtgctgc gcggggaag 
      gacaaggcc cggtttggg ggtctgatc gggtacagc 
      cgggcggaa agtccgtac tttttcgcg cccgggacg 
      gaaatggac gtggacgtg ccgctggat ttgagtatt 
      aataggaaa gtttga
    mRNA sequence
    • >TC000326 RA
      agcttgtaa atctctgga tggtcgagg gccaacccc 
      tccactacg tctaccaac ccgactact tctacccct 
      acaaaatgg aaattaacg acgacctaa tcgtcccca 
      gctcgccca acagcacac agagtgaca gtgacaact 
      ccctatcca gcatcgaag tctccccga aaccgatcc 
      gcgccagga gcgtcatcc aacacaccc ccaaaaacg 
      aggtccgct gcggctgga agtcctgcg ggatcacgt 
      tcgattccc tggaccaac tcgccacgc acgtgacga 
      aaatccacg cgtccagcg gtcccgggg gcctcttct 
      actgcggct gggagggct gttcccgca acagcaagg 
      gttttaacg ccaggtata agatgttgg tgcacgtgc 
      ggacgcaca ccaacgaga aaccgcacc agtgctgcc 
      aatgcgaga aatccttct cgcgcgccg aaaatttga 
      aaatccact cgcggtcgc acagcgggg aaaaaccgt 
      acgtgtgtc cggtcccgg ggtgcaaca aggcctact 
      cgaattcca gcgaccggt tcaagcaca cgcggacgc 
      accaggtgg agaagccgt accagtgca aggtgccag 
      ggtgcccca agcgctaca ccgacccca gcagtctcc 
      ggaagcacg tcaagacgt acaagcact tcgtcgata 
      aggtggacg agaacgcca aaagtaaca gtattgaga 
      aggtgcaag aggggcatt cgtgcgatt gcacgcaca 
      tgtgctgcg cggggaagg acaaggccc ggtttgggg 
      gtctgatcg ggtacagcc gggcggaaa gtccgtact 
      ttttcgcgc ccgggacgg aaatggacg tggacgtgc 
      cgctggatt tgagtatta ataggaaag tttgaccga 
      gtattgttt gttagagta ggacgtatt tattttatt 
      taattttgt taatggcaa taagacatt gtgataacc 
      tttttatat ttattgatg cctatttaa taaaaacta 
      aaacagttt