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Gene information
General information
TC000321 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 7531596..7534735
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000321
    iBeetle screen iB_00072
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupation - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    remarks: later than schedule, hours 24 adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: later than schedule, hours 24
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000321 RA
      MAAASSEGS PTTPNNSTQ NEKSASRSP SVSSLKEYQ 
      WTGLLVSRP VTKKILKSR GRDPVVIER SNLVNISKL 
      VVKELIEQS LRYGRMLDS DHMPLQHFF IVLEHVLRH 
      GLRPKKGLL GPKKELWDI LQMVEKYAP EAQDITASV 
      RDLPTVRTH MGRARAWLR LALMQKKLA DYLKVLIDH 
      KDEALVEYF EPDALMLSD EAIVIIGLL VGLNVIDCN 
      LCVKEEDLD CPQGVIDFS LYLRSSNHV SSDSSNDEQ 
      GDNMTTVLD QKNYIEELN RHLNATVTN LQSKVESLT 
      TMNALMKED LAIAKNSLL DLYEENRQL KQELGRDVS 
      GYCMSSSGV QITNVEPET NGKAVVTGE VDELKQRLD 
      EERKLRQEA DKELELQMC LKAEMEVAM KLLEKDIHE 
      KQDTIVSLR RQLDDIKLI NLEMYKKLQ ECEMELTQK 
      GEVVSRLQA KAGQIGKIL NNLEKYNHL LKDDKKVGD 
      RRTSEKRQA AKLQNREKV AKNVEKNES ESENKEPTT 
      SESIDSFVN LEFDGKQDE EEAKATT
    • >TC000321 RB
      MKVQEIIEG GNNAMEQEM AGAQDTIYL CNFRVSVDG 
      EWLCLKELQ DVEFNMQDS ITQPLSTPS PDVPVQPFG 
      RDPVVIERS NLVNISKLV VKELIEQSL RYGRMLDSD 
      HMPLQHFFI VLEHVLRHG LRPKKGLLG PKKELWDIL 
      QMVEKYAPE AQDITASVR DLPTVRTHM GRARAWLRL 
      ALMQKKLAD YLKVLIDHK DEALVEYFE PDALMLSDE 
      AIVIIGLLV GLNVIDCNL CVKEEDLDC PQGVIDFSL 
      YLRSSNHVS SDSSNDEQG DNMTTVLDQ KNYIEELNR 
      HLNATVTNL QSKVESLTT MNALMKEDL AIAKNSLLD 
      LYEENRQLK QELGRDVSG YCMSSSGVQ ITNVEPETN 
      GKAVVTGEV DELKQRLDE ERKLRQEAD KELELQMCL 
      KAEMEVAMK LLEKDIHEK QDTIVSLRR QLDDIKLIN 
      LEMYKKLQE CEMELTQKG EVVSRLQAK AGQIGKILN 
      NLEKYNHLL KDDKKVGDR RTSEKRQAA KLQNREKVA 
      KNVEKNESE SENKEPTTS ESIDSFVNL EFDGKQDEE 
      EAKATT
    CDS sequence
    • >TC000321 RA
      atggctgct gctagtagt gaaggttcg ccaacaact 
      ccaaataat tcaacacaa aacgaaaaa agtgcatcg 
      agatcacct tcagtatca tcactaaaa gaataccaa 
      tggaccggc ctgcttgtc tccagaccc gtcacgaaa 
      aaaattctc aaaagtcga ggtcgcgac cccgtggtc 
      atagaacgc agcaatttg gtcaacata agcaaatta 
      gtcgtcaaa gaactgata gagcagagt ctgaggtat 
      ggacgaatg ctggattcg gaccacatg cctctccaa 
      cacttcttt atagtgcta gaacacgta ttaaggcac 
      gggctacgg cctaaaaag gggcttttg gggccgaaa 
      aaggaactt tgggatatt ctacaaatg gtggagaaa 
      tacgcaccc gaagcgcag gatattacg gcaagcgtt 
      cgcgattta ccgactgtt aggacgcac atggggcga 
      gccagggcc tggctacga ctggccctc atgcagaaa 
      aaactcgcc gattattta aaagtccta attgatcat 
      aaagacgag gcacttgtt gagtacttc gaaccggat 
      gctttgatg ttgagcgat gaagccatt gtgataatc 
      gggctttta gtcggcttg aatgtcatc gattgtaac 
      ttgtgtgtt aaggaagaa gacctagat tgtccacag 
      ggtgttata gatttttcg ttatatctg agatcctcg 
      aaccacgtc tcgagcgac tcaagtaac gatgaacaa 
      ggtgataat atgacaacg gtgctagat cagaaaaac 
      tatatcgag gaacttaat cgccacctc aacgctact 
      gtcacaaat ttacaatcc aaagtcgaa tcattgacc 
      acaatgaac gcactgatg aaggaggac ctagctatt 
      gccaaaaat agtctacta gatttgtat gaagagaac 
      cggcagctg aagcaagaa ttaggcaga gacgtgagt 
      ggctattgt atgagttca agcggcgtc caaatcaca 
      aacgtcgag cccgaaacc aacggaaaa gccgtagtg 
      actggcgaa gtcgacgaa ttaaaacag cgactggac 
      gaagaacgg aaactccgc caggaagcc gacaaggag 
      ctcgaactc cagatgtgt ctcaaggcc gagatggag 
      gtggcgatg aagctcctc gagaaggac atccacgag 
      aagcaggac accatcgtg tcgctccgc cggcaactc 
      gacgacatc aaactcatc aacttggag atgtacaag 
      aagttgcag gaatgtgag atggaattg acacagaaa 
      ggggaagtg gtgagtcgt cttcaagcg aaggcgggc 
      cagatcggc aaaatcctc aataattta gaaaagtac 
      aatcacttg ttaaaagac gataagaaa gtcggcgac 
      cgaagaacg agcgaaaaa agacaagcg gcaaagttg 
      caaaatcgc gaaaaagtc gcaaagaac gttgagaaa 
      aacgagtcg gagagcgaa aataaagaa ccgaccact 
      tcggagtcg atcgattcg tttgttaat ctcgagttt 
      gacggtaaa caggacgag gaggaggcc aaagctacc 
      acataa
    • >TC000321 RB
      atgaaagtc caagaaata atcgagggc ggcaataac 
      gccatggaa caagaaatg gcgggggcg caagacacg 
      atctacctt tgcaatttt cgcgtatcg gtcgatggc 
      gaatggctc tgtctcaaa gagctccaa gacgtcgag 
      ttcaacatg caggactcc atcactcaa cccctttcc 
      acgccatcc ccggacgtc cccgtccaa cctttcggt 
      cgcgacccc gtggtcata gaacgcagc aatttggtc 
      aacataagc aaattagtc gtcaaagaa ctgatagag 
      cagagtctg aggtatgga cgaatgctg gattcggac 
      cacatgcct ctccaacac ttctttata gtgctagaa 
      cacgtatta aggcacggg ctacggcct aaaaagggg 
      cttttgggg ccgaaaaag gaactttgg gatattcta 
      caaatggtg gagaaatac gcacccgaa gcgcaggat 
      attacggca agcgttcgc gatttaccg actgttagg 
      acgcacatg gggcgagcc agggcctgg ctacgactg 
      gccctcatg cagaaaaaa ctcgccgat tatttaaaa 
      gtcctaatt gatcataaa gacgaggca cttgttgag 
      tacttcgaa ccggatgct ttgatgttg agcgatgaa 
      gccattgtg ataatcggg cttttagtc ggcttgaat 
      gtcatcgat tgtaacttg tgtgttaag gaagaagac 
      ctagattgt ccacagggt gttatagat ttttcgtta 
      tatctgaga tcctcgaac cacgtctcg agcgactca 
      agtaacgat gaacaaggt gataatatg acaacggtg 
      ctagatcag aaaaactat atcgaggaa cttaatcgc 
      cacctcaac gctactgtc acaaattta caatccaaa 
      gtcgaatca ttgaccaca atgaacgca ctgatgaag 
      gaggaccta gctattgcc aaaaatagt ctactagat 
      ttgtatgaa gagaaccgg cagctgaag caagaatta 
      ggcagagac gtgagtggc tattgtatg agttcaagc 
      ggcgtccaa atcacaaac gtcgagccc gaaaccaac 
      ggaaaagcc gtagtgact ggcgaagtc gacgaatta 
      aaacagcga ctggacgaa gaacggaaa ctccgccag 
      gaagccgac aaggagctc gaactccag atgtgtctc 
      aaggccgag atggaggtg gcgatgaag ctcctcgag 
      aaggacatc cacgagaag caggacacc atcgtgtcg 
      ctccgccgg caactcgac gacatcaaa ctcatcaac 
      ttggagatg tacaagaag ttgcaggaa tgtgagatg 
      gaattgaca cagaaaggg gaagtggtg agtcgtctt 
      caagcgaag gcgggccag atcggcaaa atcctcaat 
      aatttagaa aagtacaat cacttgtta aaagacgat 
      aagaaagtc ggcgaccga agaacgagc gaaaaaaga 
      caagcggca aagttgcaa aatcgcgaa aaagtcgca 
      aagaacgtt gagaaaaac gagtcggag agcgaaaat 
      aaagaaccg accacttcg gagtcgatc gattcgttt 
      gttaatctc gagtttgac ggtaaacag gacgaggag 
      gaggccaaa gctaccaca taa
    mRNA sequence
    • >TC000321 RA
      tccgaaatg cattcaata cataatttt tactctgct 
      tttggaata aaaaaatac agtttcctg taaacagct 
      gactttgac aacagaagt caaaatatt gacaagtaa 
      caaaaatgg ctgctgcta gtagtgaag gttcgccaa 
      caactccaa ataattcaa cacaaaacg aaaaaagtg 
      catcgagat caccttcag tatcatcac taaaagaat 
      accaatgga ccggcctgc ttgtctcca gacccgtca 
      cgaaaaaaa ttctcaaaa gtcgaggtc gcgaccccg 
      tggtcatag aacgcagca atttggtca acataagca 
      aattagtcg tcaaagaac tgatagagc agagtctga 
      ggtatggac gaatgctgg attcggacc acatgcctc 
      tccaacact tctttatag tgctagaac acgtattaa 
      ggcacgggc tacggccta aaaaggggc ttttggggc 
      cgaaaaagg aactttggg atattctac aaatggtgg 
      agaaatacg cacccgaag cgcaggata ttacggcaa 
      gcgttcgcg atttaccga ctgttagga cgcacatgg 
      ggcgagcca gggcctggc tacgactgg ccctcatgc 
      agaaaaaac tcgccgatt atttaaaag tcctaattg 
      atcataaag acgaggcac ttgttgagt acttcgaac 
      cggatgctt tgatgttga gcgatgaag ccattgtga 
      taatcgggc ttttagtcg gcttgaatg tcatcgatt 
      gtaacttgt gtgttaagg aagaagacc tagattgtc 
      cacagggtg ttatagatt tttcgttat atctgagat 
      cctcgaacc acgtctcga gcgactcaa gtaacgatg 
      aacaaggtg ataatatga caacggtgc tagatcaga 
      aaaactata tcgaggaac ttaatcgcc acctcaacg 
      ctactgtca caaatttac aatccaaag tcgaatcat 
      tgaccacaa tgaacgcac tgatgaagg aggacctag 
      ctattgcca aaaatagtc tactagatt tgtatgaag 
      agaaccggc agctgaagc aagaattag gcagagacg 
      tgagtggct attgtatga gttcaagcg gcgtccaaa 
      tcacaaacg tcgagcccg aaaccaacg gaaaagccg 
      tagtgactg gcgaagtcg acgaattaa aacagcgac 
      tggacgaag aacggaaac tccgccagg aagccgaca 
      aggagctcg aactccaga tgtgtctca aggccgaga 
      tggaggtgg cgatgaagc tcctcgaga aggacatcc 
      acgagaagc aggacacca tcgtgtcgc tccgccggc 
      aactcgacg acatcaaac tcatcaact tggagatgt 
      acaagaagt tgcaggaat gtgagatgg aattgacac 
      agaaagggg aagtggtga gtcgtcttc aagcgaagg 
      cgggccaga tcggcaaaa tcctcaata atttagaaa 
      agtacaatc acttgttaa aagacgata agaaagtcg 
      gcgaccgaa gaacgagcg aaaaaagac aagcggcaa 
      agttgcaaa atcgcgaaa aagtcgcaa agaacgttg 
      agaaaaacg agtcggaga gcgaaaata aagaaccga 
      ccacttcgg agtcgatcg attcgtttg ttaatctcg 
      agtttgacg gtaaacagg acgaggagg aggccaaag 
      ctaccacat aatcatttt aagattttg cttgtctgt 
      gtaatgttt tatttgttg aaatatctg gaacgggag 
      ttgcagatg tttcgatta tttttattt attgaaaga 
      aatttgaag actgagatt gggataaaa gtgtgactt 
      atttcagga atgcgaagc atctttaaa gcataagtc 
      ggaacttgt aaccaagtt agaggctaa ggcacagtc 
      aatgacaga cacaatgca aacacttga acttaagta 
      ggtattgat ttgcttact gtttgggta gattttgct 
      ttcagtact agactaatt catacttgt aacaaacat 
      tctacaaca gtgttgtac aatgtccta attgtaaga 
      cctttgtat tggaaatgt tggcaaacc ttcactaca 
      gcagctact agtaaactt gtttatcat gttaccgta 
      gaataattg taagtgtta tattcttat aattctatg 
      ttatcgcaa acatcagtt tatacttta tgaaccgtt 
      tcgcttgta aaaataaac ctaatcaac caac
    • >TC000321 RB
      tccgaaatg cattcaata cataatttt tactctgct 
      tttggaata aaaaaatac agtttcctg taaacagct 
      gactttgac aacagaagt caaaatatt gacaagtaa 
      caaaaatgg ctgctgcta gtagtgaag gttcgccaa 
      caactccaa ataattcaa cacaaaacg aaaaaagtg 
      catcgagat caccttcag tatcatcac taaaagaat 
      accaatgga ccggcctgc ttgtctcca gacccgtca 
      cgaaaaaaa ttctcaaaa gtcgagttt tatcaaaag 
      cgggctcac ttgagttga aaaaaaaga cgctacagt 
      tgttacgta cgcacattg taaatttct aaaaaagtt 
      tcaaaaaat tgcaattgc gatttatgg gttgttcca 
      cgcgcaccc tgaccaccc ccattgttt aaaaaaatc 
      gccaaaaca gcaacaacg tcgaccttt cccaaacat 
      tcgcgcaca agcgcattc cacccccac agtccaccc 
      ttgcgatca ataaaccga gtgcgcttt ccagaacgt 
      agcgatgtt tcgcgttac caaaaaccc gtgtgttta 
      atttcccct attgtttta aacataaaa cactcatga 
      aagtccaag aaataatcg agggcggca ataacgcca 
      tggaacaag aaatggcgg gggcgcaag acacgatct 
      acctttgca attttcgcg tatcggtcg atggcgaat 
      ggctctgtc tcaaagagc tccaagacg tcgagttca 
      acatgcagg actccatca ctcaacccc tttccacgc 
      catccccgg acgtccccg tccaacctt tcggtcgcg 
      accccgtgg tcatagaac gcagcaatt tggtcaaca 
      taagcaaat tagtcgtca aagaactga tagagcaga 
      gtctgaggt atggacgaa tgctggatt cggaccaca 
      tgcctctcc aacacttct ttatagtgc tagaacacg 
      tattaaggc acgggctac ggcctaaaa aggggcttt 
      tggggccga aaaaggaac tttgggata ttctacaaa 
      tggtggaga aatacgcac ccgaagcgc aggatatta 
      cggcaagcg ttcgcgatt taccgactg ttaggacgc 
      acatggggc gagccaggg cctggctac gactggccc 
      tcatgcaga aaaaactcg ccgattatt taaaagtcc 
      taattgatc ataaagacg aggcacttg ttgagtact 
      tcgaaccgg atgctttga tgttgagcg atgaagcca 
      ttgtgataa tcgggcttt tagtcggct tgaatgtca 
      tcgattgta acttgtgtg ttaaggaag aagacctag 
      attgtccac agggtgtta tagattttt cgttatatc 
      tgagatcct cgaaccacg tctcgagcg actcaagta 
      acgatgaac aaggtgata atatgacaa cggtgctag 
      atcagaaaa actatatcg aggaactta atcgccacc 
      tcaacgcta ctgtcacaa atttacaat ccaaagtcg 
      aatcattga ccacaatga acgcactga tgaaggagg 
      acctagcta ttgccaaaa atagtctac tagatttgt 
      atgaagaga accggcagc tgaagcaag aattaggca 
      gagacgtga gtggctatt gtatgagtt caagcggcg 
      tccaaatca caaacgtcg agcccgaaa ccaacggaa 
      aagccgtag tgactggcg aagtcgacg aattaaaac 
      agcgactgg acgaagaac ggaaactcc gccaggaag 
      ccgacaagg agctcgaac tccagatgt gtctcaagg 
      ccgagatgg aggtggcga tgaagctcc tcgagaagg 
      acatccacg agaagcagg acaccatcg tgtcgctcc 
      gccggcaac tcgacgaca tcaaactca tcaacttgg 
      agatgtaca agaagttgc aggaatgtg agatggaat 
      tgacacaga aaggggaag tggtgagtc gtcttcaag 
      cgaaggcgg gccagatcg gcaaaatcc tcaataatt 
      tagaaaagt acaatcact tgttaaaag acgataaga 
      aagtcggcg accgaagaa cgagcgaaa aaagacaag 
      cggcaaagt tgcaaaatc gcgaaaaag tcgcaaaga 
      acgttgaga aaaacgagt cggagagcg aaaataaag 
      aaccgacca cttcggagt cgatcgatt cgtttgtta 
      atctcgagt ttgacggta aacaggacg aggaggagg 
      ccaaagcta ccacataat cattttaag attttgctt 
      gtctgtgta atgttttat ttgttgaaa tatctggaa 
      cgggagttg cagatgttt cgattattt ttatttatt 
      gaaagaaat ttgaagact gagattggg ataaaagtg 
      tgacttatt tcaggaatg cgaagcatc tttaaagca 
      taagtcgga acttgtaac caagttaga ggctaaggc 
      acagtcaat gacagacac aatgcaaac acttgaact 
      taagtaggt attgatttg cttactgtt tgggtagat 
      tttgctttc agtactaga ctaattcat acttgtaac 
      aaacattct acaacagtg ttgtacaat gtcctaatt 
      gtaagacct ttgtattgg aaatgttgg caaaccttc 
      actacagca gctactagt aaacttgtt tatcatgtt 
      accgtagaa taattgtaa gtgttatat tcttataat 
      tctatgtta tcgcaaaca tcagtttat actttatga 
      accgtttcg cttgtaaaa ataaaccta atcaaccaa 
      c