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Gene information
General information
TC000316 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 7578971..7586490
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000316
    iBeetle screen iB_00069
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    thorax partially constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    Adult morphology
    motility irregular - (22 dpi)
    Days post injection

    Comment: Legs are correctly formed, but can't be streched out. Joints between femur and tibia are not moveable.  

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000316 RA
      MWRLLGFGV VLAAVAAPN NQVPKTGFS CEGRTTGYY 
      ADVESGCQV YHMCDGLGR QFSYTCPNA TLFQQRMLI 
      CDHWYMVNC SKSVDDYTA NLRIGHKEM PFVDDNEAN 
      PYHRTPRPD LLSHPSQSE YDIIYRTGR AQLGTNLNL 
      VGAESDPKN STTSTTDEP AYSLPSHWS TEYNKQVTT 
      TKPKPAKKG KPTTVRVNY QSNYKATTP VFPQSVEVT 
      EAPDLELVP PSGSTESSV NFESRFKAT TPVFPLSVD 
      VTEAPNLGL LPPLPYQLN DTKPNENLV VSTVNFESK 
      FKATTPVYP KSVELTSEE PSEVGVLPP KDTNSTETD 
      VVPPLSIDF EPPVVDSNF KPGDHSEEI PPELPSKFY 
      QPPKFEPDY TELAKLKGG FKALSSGEW EDLRKKFLI 
      PDYEFPLET VSRPSYDSV LSSFQVGPV PSEKE
    CDS sequence
    • >TC000316 RA
      atgtggcgg cttttagga tttggagtc gttctggcc 
      gccgtcgcg gcgcccaac aaccaggtt cctaagact 
      ggcttttcc tgcgagggc cgcaccacg gggtactac 
      gccgacgtg gaaagcggc tgccaagtc taccacatg 
      tgtgacggt ttaggccgc cagttcagc tacacttgt 
      cccaatgca actctcttc caacagaga atgttgatt 
      tgcgaccac tggtacatg gtcaactgt agcaaatct 
      gtggatgat tacacggcc aatttgcgc atcgggcac 
      aaggaaatg cccttcgtt gacgacaac gaagccaat 
      ccttaccac aggaccccc aggcctgac ctcctctcg 
      caccccagt cagtccgaa tacgacatt atctaccgc 
      accggaagg gcgcagctt ggcaccaat ctgaatcta 
      gtcggcgcc gaatcagac cccaaaaac agtaccacc 
      agtaccaca gacgaaccc gcctattct ctacccagc 
      cactggtca acggaatat aacaaacaa gtgaccaca 
      actaaacca aaaccggcc aaaaagggg aaacccacc 
      acagtcagg gtgaactac cagtctaat tataaagcc 
      acgactcct gtgttcccc caaagtgtg gaggtgacc 
      gaagccccc gatttggaa ctagtgccc ccttcgggg 
      agtaccgaa agctcggtt aatttcgag tcgaggttc 
      aaggctacg actccggtg tttccccta agtgtggac 
      gtgacggaa gccccaaat ttgggactt ctgccacct 
      ctaccctac caacttaac gacacgaaa ccaaacgaa 
      aatttagtc gttagtacg gtcaatttc gagtcaaaa 
      ttcaaggcc acgacccca gtctacccc aaaagtgtc 
      gaactgacg agtgaagaa ccgtccgaa gttggggtt 
      ttgcccccc aaagacacc aattcgact gaaactgat 
      gttgtgccc cctttaagt atagatttt gagccgcct 
      gttgtcgac tctaatttc aagccaggt gatcacagt 
      gaggaaata ccaccagaa ttaccgtca aagttctac 
      caacccccg aaatttgaa cctgactat accgaatta 
      gcgaagcta aagggtggg tttaaggcg cttagcagc 
      ggcgaatgg gaggatttg aggaagaag ttcctgatt 
      cccgattac gagtttccc ttagagacc gtttcaagg 
      cctagttat gacagtgtt ttgagttcg tttcaagtc 
      ggaccagtg ccatccgag aaagagtga
    mRNA sequence
    • >TC000316 RA
      gccgagttg tagatttgt agtgggacg ggttgtatc 
      gtgaagtgt gaagaacgg tacctgaaa tgtggcggc 
      ttttaggat ttggagtcg ttctggccg ccgtcgcgg 
      cgcccaaca accaggttc ctaagactg gcttttcct 
      gcgagggcc gcaccacgg ggtactacg ccgacgtgg 
      aaagcggct gccaagtct accacatgt gtgacggtt 
      taggccgcc agttcagct acacttgtc ccaatgcaa 
      ctctcttcc aacagagaa tgttgattt gcgaccact 
      ggtacatgg tcaactgta gcaaatctg tggatgatt 
      acacggcca atttgcgca tcgggcaca aggaaatgc 
      ccttcgttg acgacaacg aagccaatc cttaccaca 
      ggaccccca ggcctgacc tcctctcgc accccagtc 
      agtccgaat acgacatta tctaccgca ccggaaggg 
      cgcagcttg gcaccaatc tgaatctag tcggcgccg 
      aatcagacc ccaaaaaca gtaccacca gtaccacag 
      acgaacccg cctattctc tacccagcc actggtcaa 
      cggaatata acaaacaag tgaccacaa ctaaaccaa 
      aaccggcca aaaagggga aacccacca cagtcaggg 
      tgaactacc agtctaatt ataaagcca cgactcctg 
      tgttccccc aaagtgtgg aggtgaccg aagcccccg 
      atttggaac tagtgcccc cttcgggga gtaccgaaa 
      gctcggtta atttcgagt cgaggttca aggctacga 
      ctccggtgt ttcccctaa gtgtggacg tgacggaag 
      ccccaaatt tgggacttc tgccacctc taccctacc 
      aacttaacg acacgaaac caaacgaaa atttagtcg 
      ttagtacgg tcaatttcg agtcaaaat tcaaggcca 
      cgaccccag tctacccca aaagtgtcg aactgacga 
      gtgaagaac cgtccgaag ttggggttt tgcccccca 
      aagacacca attcgactg aaactgatg ttgtgcccc 
      ctttaagta tagattttg agccgcctg ttgtcgact 
      ctaatttca agccaggtg atcacagtg aggaaatac 
      caccagaat taccgtcaa agttctacc aacccccga 
      aatttgaac ctgactata ccgaattag cgaagctaa 
      agggtgggt ttaaggcgc ttagcagcg gcgaatggg 
      aggatttga ggaagaagt tcctgattc ccgattacg 
      agtttccct tagagaccg tttcaaggc ctagttatg 
      acagtgttt tgagttcgt ttcaagtcg gaccagtgc 
      catccgaga aagagtgaa ggggaaaaa gagtttttt 
      cctttaaag tctgatacc aaagttggg taatagcat 
      gagatactc atttttcaa attatgatt gtaaataat 
      agatccgtt gtgtttaat aacagttgg aaaaaccag 
      tcaattata atgccacaa ccattgtta caagcgtct 
      gatacgtgt aaaatgcgc acgatgaaa tagctttaa 
      ttaaatcgg gttatataa ctgtgtcgc cattttttt 
      aaggactca cgtttaatt tatactttt gtaaaacgt 
      aattaaata aagaatgtt aagagga