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Gene information
General information
TC000315 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13122046..13135750
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000315
    iBeetle screen iB_00068

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    pupation - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    remarks: later than schedule, hours 24 adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000315 RA
      MDYKSVVYN AARDGNLNR LKFYLHGNK GKEEVSMLV 
      AAKTSGATP LVIACRNGH YDVAEYLIE RCHADIEQP 
      GSVMFDGET IEGAPPLWC AAAAGHLDI VKLLISHGA 
      KVNSTTRTN STPLRAACF DGHLDIVKY LVQHGADIE 
      VANRHGHSC LMIACYKGH IRIARYLLS LKASVNRKS 
      VKGNTALHD CAESGSLEI LKLLIDNGA TMDVDSYGM 
      TPLLAAAVT GHNHIVEYL IKLPHLVSR KEKIDALEL 
      LGATLVDKK RDMVGALEV WKRAMHYRY HGDGPPILK 
      PRIQKVPAY DYVVEITDL DALDDLMAD PDEMRMQAL 
      VMRERILGP AHPDTSYYI RYRGAVYAD AGKFNRCIE 
      LWNYALDMQ QSMLERLNP MTQSSLFSF TELFSFIMG 
      EEGKHTTRG RLVPPVDVV EILRVFKKA VKEVELGSQ 
      MLEKMPNVE RDMTYLTRV LVITLHLAC LLTRMLEHH 
      TTTEAITKE IYKAVYDLV KLKIRGRSG RTALHLACC 
      RDAALLGRY PACQFPSAH LAEVLLKVG ADPNVKDDD 
      GNTPFHLAA MARPCPANI AQVLLKHGA HIDLVNNNG 
      ETFASLLKG QQVHELVNT AKYTGLSCA AARIIQQFK 
      IPYKGIVPA ALESFIACH
    CDS sequence
    • >TC000315 RA
      atggattac aaaagcgtc gtttacaac gccgctaga 
      gacggaaat ctaaacagg cttaagttt tacctgcac 
      ggcaacaaa ggcaaggag gaggtttcc atgctcgtg 
      gcggcgaag acctcgggg gccacgccg ttggtgatc 
      gcctgccgg aacggccac tacgacgtc gccgagtac 
      ctcatcgag cgctgccat gccgacatc gagcagccc 
      ggctcagtg atgttcgac ggcgagacc atcgagggc 
      gcgcccccg ctgtggtgc gcggcggcc gcgggccac 
      ctggacatc gtcaagctg ctgatttcc cacggcgcc 
      aaagtcaac agcacgacc aggaccaac tcgacgccg 
      ctgcgggcc gcctgcttc gacgggcac ttggacatc 
      gtcaagtac ctggtgcag cacggcgcc gacatcgag 
      gtggccaac aggcacggg cattcctgt ctgatgatt 
      gcgtgctac aagggccat attcggatc gctagatat 
      ttacttagt ttgaaggct agtgttaat aggaaaagt 
      gtcaaaggt aacacggcg ctgcatgat tgtgccgag 
      tccgggagt ctggaaatt ctcaaattg cttattgat 
      aatggagcg acgatggat gttgattct tacggaatg 
      acgccgttg ctggcagct gcagttact ggacataat 
      catattgta gaatattta attaaactt ccgcatctc 
      gtatcgagg aaggaaaaa atcgatgcg ttagagttg 
      ttaggagcc acgcttgtt gataagaaa cgtgatatg 
      gtcggtgct ttagaagtg tggaagcgg gcaatgcat 
      tacaggtac cacggtgac ggtccgccg attctaaaa 
      ccccgcatc cagaaagtc cccgcctac gactacgtg 
      gtcgaaatc accgatctg gacgctctg gacgaccta 
      atggccgat ccagacgaa atgcgcatg caagcccta 
      gtaatgcgt gagcgtatc ttaggcccc gctcacccc 
      gacaccagc tactacatc cgttaccgt ggggctgtt 
      tacgccgac gctggcaaa ttcaaccgt tgcatcgag 
      ttgtggaac tacgccctc gacatgcaa caatccatg 
      ctcgagcgt ttaaacccc atgacccaa agcagctta 
      ttctccttc accgaatta ttctcgttc ataatgggc 
      gaggaaggc aaacacacg acacgtggt cgcctcgtc 
      ccgcccgtc gatgtcgtc gaaatttta cgagttttc 
      aagaaagca gtcaaggaa gtagaacta ggtagtcaa 
      atgttggag aagatgccc aatgttgag cgagacatg 
      acgtatctg acccgcgtt ttggtcata actctacac 
      ttggcctgc ttgttaaca cgtatgctt gagcaccat 
      actacgact gaagcaatc acgaaggag atttacaag 
      gcggtgtac gatttagtc aagttgaag ataaggggg 
      cgctcgggg cgcacggcc cttcacttg gcgtgctgt 
      cgggacgcc gcccttctg gggcgctac cccgcctgt 
      cagttccct tcggcgcat ctggccgag gttttgctc 
      aaagtgggg gccgatccg aacgtgaag gacgacgac 
      gggaacacg ccgttccat ctagcggcc atggcacgc 
      ccctgtccg gccaacata gcccaagtg ctgctcaaa 
      cacggggcg cacatcgac ctagtgaac aataacggt 
      gagactttc gctagtttg ctcaaagga cagcaagtg 
      cacgagtta gtgaacacg gccaaatac acgggactg 
      agttgtgca gccgcgaga attatccaa cagtttaaa 
      ataccctat aaaggaatc gtaccagca gctttagaa 
      tcgtttatt gcatgccac tga
    mRNA sequence
    • >TC000315 RA
      gtagtctgt cagctgcag cgtcgtgct gtaggcaat 
      ggcttcgta gctttttgt taaaaaagc cacaatttc 
      acaaaatcc cgactttgt tctgtgaaa ggtgtaggc 
      caaaatgga ttacaaaag cgtcgttta caacgccgc 
      tagagacgg aaatctaaa caggcttaa gttttacct 
      gcacggcaa caaaggcaa ggaggaggt ttccatgct 
      cgtggcggc gaagacctc gggggccac gccgttggt 
      gatcgcctg ccggaacgg ccactacga cgtcgccga 
      gtacctcat cgagcgctg ccatgccga catcgagca 
      gcccggctc agtgatgtt cgacggcga gaccatcga 
      gggcgcgcc cccgctgtg gtgcgcggc ggccgcggg 
      ccacctgga catcgtcaa gctgctgat ttcccacgg 
      cgccaaagt caacagcac gaccaggac caactcgac 
      gccgctgcg ggccgcctg cttcgacgg gcacttgga 
      catcgtcaa gtacctggt gcagcacgg cgccgacat 
      cgaggtggc caacaggca cgggcattc ctgtctgat 
      gattgcgtg ctacaaggg ccatattcg gatcgctag 
      atatttact tagtttgaa ggctagtgt taataggaa 
      aagtgtcaa aggtaacac ggcgctgca tgattgtgc 
      cgagtccgg gagtctgga aattctcaa attgcttat 
      tgataatgg agcgacgat ggatgttga ttcttacgg 
      aatgacgcc gttgctggc agctgcagt tactggaca 
      taatcatat tgtagaata tttaattaa acttccgca 
      tctcgtatc gaggaagga aaaaatcga tgcgttaga 
      gttgttagg agccacgct tgttgataa gaaacgtga 
      tatggtcgg tgctttaga agtgtggaa gcgggcaat 
      gcattacag gtaccacgg tgacggtcc gccgattct 
      aaaaccccg catccagaa agtccccgc ctacgacta 
      cgtggtcga aatcaccga tctggacgc tctggacga 
      cctaatggc cgatccaga cgaaatgcg catgcaagc 
      cctagtaat gcgtgagcg tatcttagg ccccgctca 
      ccccgacac cagctacta catccgtta ccgtggggc 
      tgtttacgc cgacgctgg caaattcaa ccgttgcat 
      cgagttgtg gaactacgc cctcgacat gcaacaatc 
      catgctcga gcgtttaaa ccccatgac ccaaagcag 
      cttattctc cttcaccga attattctc gttcataat 
      gggcgagga aggcaaaca cacgacacg tggtcgcct 
      cgtcccgcc cgtcgatgt cgtcgaaat tttacgagt 
      tttcaagaa agcagtcaa ggaagtaga actaggtag 
      tcaaatgtt ggagaagat gcccaatgt tgagcgaga 
      catgacgta tctgacccg cgttttggt cataactct 
      acacttggc ctgcttgtt aacacgtat gcttgagca 
      ccatactac gactgaagc aatcacgaa ggagattta 
      caaggcggt gtacgattt agtcaagtt gaagataag 
      ggggcgctc ggggcgcac ggcccttca cttggcgtg 
      ctgtcggga cgccgccct tctggggcg ctaccccgc 
      ctgtcagtt cccttcggc gcatctggc cgaggtttt 
      gctcaaagt gggggccga tccgaacgt gaaggacga 
      cgacgggaa cacgccgtt ccatctagc ggccatggc 
      acgcccctg tccggccaa catagccca agtgctgct 
      caaacacgg ggcgcacat cgacctagt gaacaataa 
      cggtgagac tttcgctag tttgctcaa aggacagca 
      agtgcacga gttagtgaa cacggccaa atacacggg 
      actgagttg tgcagccgc gagaattat ccaacagtt 
      taaaatacc ctataaagg aatcgtacc agcagcttt 
      agaatcgtt tattgcatg ccactgaat ttgtgatgt 
      gtttttaat gtacaacgt ttggcagca tctacaatt 
      tttagtcgg tttggcgcg tccattcaa agtgaggtt 
      ggcggccat gattggttc aattgaact aacttcatt 
      tttgtaaat aaattaaaa ttgcaaatt ccacgagaa 
      aaaacaaga aatcgaaac gttttaaac cgcgggaca 
      ccccatgtg cttaattgt tgttaaaac ttgtgcaaa 
      cgtctcgga taaatggcc aaggtacgt cctaacctc 
      gcttttatt tcaagtaaa ttactcgag tggacgcta 
      tttaataaa ttctaatca tttagacct acagttagg 
      ataattgga agtttatta aataatacc aaaacagcg 
      ttccacaag taatttata aattcgcga tttaaattg 
      cagctccct ctcattagc gggaaatta aattatgca 
      ggggggc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY