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Gene information
General information
TC032222
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000310,TC000311
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 7649231..7664497
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032222
    iBeetle screen iB_00067
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    abdomen segment number # of remaining segments increased - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: Most animals die as L2 larvae
    posterior terminus irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    urogomphi potentially transformed - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    Pupal morphology
    oblique dorsal thoracic muscles shortened - Number of animals showing the phenotype: 6
    oblique dorsal thoracic muscles sometimes orientation irregular - Number of animals showing the phenotype: 6
    oblique dorsal thoracic muscles broader - Number of animals showing the phenotype: 5

    female genital lobes shortened - Number of animals showing the phenotype: 8

    female genital lobes potentially duplicated - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032222 RA
      MAGNEATSQ TELWSSVVH QPCEEPEES ELPSTVDMA 
      AFSTGLTVE PANTSQNNE NSDSDSDSE PESSNSSEA 
      CQSSSQSDS SDSDSSSSS VESSSRSPS PEFSVTTSQ 
      TNSLRLTIA TVRKSSGSP PVDKMTKPA DSKPAKSGD 
      CSSSSDSVC SSDSESDSE ASGAKSSVK TSVKSAKDN 
      NVRRSAARL RTRVPDKKD EKKAKKEAA PAKGPVTRP 
      RTRQRKTKK MAASLPLSR GSTYSSSDS EDSDADRAI 
      LASCSLQEI RQEDLAAIL PDQQECDAF GDFECNRVD 
      KNSPQTGDM SSSDMELPQ QAVNALIQR TTESSSESE 
      SHAPPNPST LYANSLLQQ FVAQTQMLN APCPVIPTG 
      TDGIKTLPL NTCENQSNN NNTTNNNNN NTGGDTVKR 
      KRGRPRKNH KTQNDNKTQ NKTIEYCGD PNVSPDSGI 
      QNSPDHVSS PEPTLTLKN KEENKKDNK TKTVNKSKE 
      INKLSVTSN RFDRLLYAN TDRVLYPPR RKVGRPPLT 
      KKGPGRPPK YKPPANTNQ ETKPPDKIK PKTEPRPVL 
      VTKNKIDNT KVKFTTLKN LKVMHSKHK HKKHKKYKI 
      KILKPLTGT NDKVVIEID KLVSDFTKF CVIGSTKPP 
      KENVPEVKP AKKVSKKRK TTEQKKKKI SVNATVNKV 
      ANLNEQRLP LKKRHYLYS DGKKSEEIL PKEEKVSAN 
      NNSITATTP KKRHRLEVI NKEKENAAV PAVEPKVKP 
      KLSLESLIS ELKMKRGLS GQEDSLDAV KVDSVVSPD 
      AKTKTTVEN NLKKKPRKR RAINRTGFP TVKKKKKKV 
      VSVEDSVDT EEDKPLISC DRVPKDGEE YNKFIQRTE 
      NADASKTDD SLSKWEVMS ECDSLPQDE RIEDKGDSG 
      GDMDEVSLE ASIERLRSR LDKKKRKRA EKIVDLNGY 
      PKRRLRDIS PASSIDVII ERRLREERA SASGDEMKK 
      NKKAPRWRK RYLVAGLFS DYFKEDDET CRIKRSENA 
      AKSRLVYNP AEHPYGLLP PPYHCGKYL RCRKLPFQL 
      PYDLWWQHT HSQLPGRDV VPSWNYRKI RTNVYNVKT 
      TTGACEPQT CNCTPLANC GDDCINRLV LAECPASHK 
      CQNQKIQRH EWAPGLEKF MTENKGWGV RTKLPIKSG 
      EFILEYVGE VVSDQEFKE RMATIYVND THHYCLHLD 
      GGLVIDGHR MGGDGRFVN HSCQPNCEM QKWSVNGQF 
      RMALFALRD IESSEELTY DYNFSLFNP AEGQECKCG 
      SEMCRGVIG GKSQRVRQL PEVKDGKNP GRVGRPRKN 
      QAKRTTSVK DLPLAQPSV PIVAPPQVK PMSHQQKCF 
      ILEHHCFLL RNLTKVKRV RDRSSSSLI SRQQAMTPT 
      NQGAAFINH LNALGKPRN IRTRRLAQA EDNPELSKT 
      VKLAAVLRE IWNTVTKAK DENNESLST HFMTMPSKR 
      KVPEFYQRI TNPIDLATI EQNIATGYY LAPESFNSD 
      MNRMFANYL QFYGRTKEI GVAAIKLKK VYVDAKQQS 
      LPKFENVLG KKPPNFVSN RNKCKDEEE DIIRCICGM 
      PRDEGLMIQ CERCMVWQH SECVKADVS AESYHCEQC 
      VPREVDYEI PLDEFTEHG HRYYMTLLR GELQLRQGD 
      TVYVLRDIP IPGTDRKHT YDTIGKIEY SELDIFRIE 
      RLWKDSKTG QRFAYGHHY LRPHETYHE PTRKFFPNE 
      VMRVPLYEA VPVELIISH CWVMDVNTF CKGRPIGAP 
      EEHIYICEY RVDKSARLF SKVSKSKFP ICTKSFAFE 
      RFETRLKVS RTYTPHDLD PALVKPRGR KPQETEDRG 
      ETVIPPPLP VPVPVIRTP VEQKARLNK ILLNLLSKM 
      PTKQALDVS YLLEGGRRR KKPVENGKN
    CDS sequence
    • >TC032222 RA
      atggcagga aatgaagca accagccaa acagaatta 
      tggtcttcg gtggtccac caaccatgc gaggagccc 
      gaggagtca gaattgccc agtacggtg gacatggcc 
      gctttcagt accggacta accgtagaa cccgccaac 
      acatcgcaa aataatgag aattccgac agtgacagc 
      gactcagaa cccgaatct agtaattca agtgaagct 
      tgccaatct tccagccag agcgactct tctgattct 
      gacagttcc tcgtccagt gttgaatca tcgtcaagg 
      agcccctcg ccggaattt tcagtcacc acttcccaa 
      accaacagt ttaaggctc acaattgcc actgtaagg 
      aagtcatca ggaagtcca ccggttgat aaaatgacc 
      aaaccggct gactctaaa ccggcgaaa agtggtgac 
      tgcagctcc tcgtcagac tcagtgtgt tcttctgat 
      tccgaaagc gattcggaa gcttccggt gctaaaagt 
      tcagttaag acgagtgtg aaaagtgcc aaggataat 
      aatgttagg agaagtgcc gcgagactg cgaactaga 
      gtgcctgat aagaaagac gaaaagaag gccaagaag 
      gaggcggcg cccgccaag ggccccgtc accaggcct 
      aggaccagg caaaggaag accaaaaag atggcggca 
      tcgttgcct ctatcccgt ggctcgacc tattccagc 
      tcggactcg gaggattcg gacgccgat agggccatt 
      ctagcctcc tgcagtttg caggaaata cgccaggag 
      gatctagca gcgatttta cccgaccag caggaatgc 
      gacgccttc ggcgacttt gaatgtaac agggttgat 
      aaaaattca ccacaaacc ggtgacatg tccagctct 
      gacatggag ctgccacag caagcggtc aatgccctc 
      atacagaga actacagaa agttccagc gaatccgaa 
      tcgcatgca ccccctaat ccctcaact ctttatgcc 
      aacagtttg ctacaacaa ttcgtcgct caaacccaa 
      atgctaaat gcaccgtgt cctgtaata ccgacaggg 
      accgatggc attaaaacc ctacccctt aacacttgc 
      gaaaatcaa agtaataac aataatacc acgaataat 
      aataataac aacacagga ggtgatacg gttaaaagg 
      aaaagagga cggccgaga aaaaatcac aagacacaa 
      aatgataac aaaactcaa aataaaaca attgagtat 
      tgtggtgat cctaacgtt tcacccgat tccgggatt 
      cagaatagt cccgaccac gtctcaagt cccgaacca 
      accctaact ttaaaaaac aaagaagag aataaaaaa 
      gacaacaaa acaaaaact gtcaataaa tcgaaagaa 
      ataaacaaa ttatcagtg acttcgaat cggttcgat 
      cgcttgttg tatgctaac accgaccgg gttctctac 
      ccgccccga aggaaagta ggccggcct ccgttaaca 
      aaaaaaggc ccaggtagg ccccccaaa tacaaacca 
      ccagccaac acaaaccaa gaaacaaaa ccgcccgat 
      aaaattaaa ccaaaaacc gaaccacgg cccgtactt 
      gtaaccaaa aacaaaatc gacaatacc aaagtgaaa 
      ttcacaaca ttgaaaaat ctcaaagtg atgcacagc 
      aaacacaaa cacaagaaa cacaaaaaa tacaaaatc 
      aaaatcctc aaaccattg accggaacc aacgacaaa 
      gtcgtgatc gaaatcgat aaactagtg tcagacttt 
      acgaaattt tgcgtgatt ggaagtacg aaaccgccg 
      aaagagaac gtacccgaa gtgaaaccg gcgaagaaa 
      gtgtcgaaa aagcgcaaa accacggaa cagaagaag 
      aagaagatt agtgtgaat gccaccgtt aataaagtg 
      gcgaattta aacgagcag agattgccg ttaaaaaag 
      cgacattac ttgtattcc gatggcaag aaaagcgag 
      gagattctc ccgaaagag gagaaagtc agtgcgaat 
      aataattcc ataacggcg accacgccg aagaagcga 
      caccgactg gaggtgatc aacaaggag aaggaaaat 
      gcggccgtt ccggcggtc gagcctaaa gtcaagccc 
      aaactgtct ctagaatcc ctcatttcc gaattgaag 
      atgaagaga gggttgtca ggtcaggag gacagtttg 
      gacgcggtc aaagtcgac tcggttgtc agtccggac 
      gctaaaact aaaaccacc gttgagaat aatttgaag 
      aagaagccg cggaagcgg cgcgccatc aaccggacc 
      gggttcccc actgtcaag aagaagaag aagaaggtg 
      gtttcggtg gaagattcg gtcgatacc gaggaagat 
      aagcctttg attagctgt gatcgcgtc ccgaaagac 
      ggcgaagag tacaataaa ttcattcaa aggacggaa 
      aatgccgac gcgtccaaa accgacgat tccctatcg 
      aagtgggaa gtgatgagc gagtgtgat agtctcccg 
      caggatgaa cgcatcgag gataagggg gacagtggc 
      ggggacatg gacgaggtc tcacttgag gcgagcatt 
      gaacggttg aggtcacga cttgataaa aagaagcgg 
      aaacgtgcg gaaaaaatc gtcgatttg aacggttat 
      cccaaaagg cggcttagg gacatatcc ccagctagt 
      agtattgat gttatcatc gagcgccgc ttacgcgag 
      gaacgagct tccgcctcc ggcgacgaa atgaagaag 
      aacaagaag gcgccaagg tggcgcaag cgctacctg 
      gttgctggt cttttctcc gattatttc aaagaggac 
      gacgagact tgtaggatt aaaaggagc gaaaacgcg 
      gcgaagtcc cgcctggtg tacaacccg gccgagcat 
      ccgtacggt cttttaccg ccgccctat cactgcggt 
      aaatattta cggtgtcgg aagcttccc ttccagtta 
      ccgtacgac ttgtggtgg caacatacg cattcgcag 
      ttgccaggc agggatgtt gtaccgtct tggaattat 
      cggaaaata cggacgaat gtttataat gttaagacg 
      acgacggga gcgtgcgaa ccacaaact tgcaattgt 
      acgccgttg gctaattgt ggtgacgat tgtattaat 
      cgacttgtg ttggccgaa tgtcccgcc agccacaag 
      tgccaaaac cagaagatt cagaggcat gagtgggcg 
      ccggggctt gagaaattc atgaccgaa aataagggt 
      tggggcgtg cgcacgaaa ctccccatc aagtccggg 
      gaattcatc ctggagtac gtgggcgag gtggtgtcc 
      gaccaggaa ttcaaagaa cgcatggct accatttac 
      gtcaacgac acccaccac tactgcttg cacctagac 
      ggtggtttg gtcatcgac ggccaccga atgggcggt 
      gacggccgt ttcgtcaat cattcctgt caaccaaac 
      tgcgagatg cagaaatgg tcagtcaac ggccagttc 
      cgaatggct ttattcgcc ttgcgcgac attgaaagt 
      tccgaagaa ctaacatac gactacaac ttctcactt 
      ttcaatccg gccgaaggc caggaatgc aaatgcggc 
      agcgaaatg tgccgtggc gtcatcgga ggcaagtcg 
      caaagagtg cgccaactg ccggaagtc aaagacggg 
      aaaaatcca ggacgtgtg ggacgaccg cgaaaaaat 
      caagccaag cggactaca agtgttaaa gatctgcca 
      ttggcgcag ccaagcgtg ccgatagtg gcgcccccg 
      caagtcaag ccgatgagt caccaacag aaatgtttc 
      attctggag caccactgt ttcctctta cggaaccta 
      acgaaggtg aaacgggtg cgggatagg tcgagcagt 
      tcgctcatt tcgcgccaa caagccatg acgccgacc 
      aatcaaggc gctgcgttt atcaatcat ttgaacgcg 
      ttgggaaaa ccgcggaat attcggacc aggcgattg 
      gcccaagcc gaggacaat cccgagttg agtaaaaca 
      gtgaaactt gccgccgtg ttacgggag atttggaac 
      acagtgacg aaggccaaa gacgagaat aacgaatcg 
      ttgagtacg cattttatg acaatgcca tcgaaacgc 
      aaagtacct gagttctat caacgcatt actaatccg 
      attgatctc gccaccatc gagcaaaac atcgccact 
      ggctattac ctggcgcct gaaagcttc aattcggat 
      atgaatcga atgtttgcc aattatttg cagttttac 
      gggcggacg aaggaaatc ggcgttgct gcgattaaa 
      ttgaagaaa gtctacgtc gatgccaaa caacaaagt 
      ttgcccaaa ttcgaaaac gtgttagga aaaaaacca 
      ccgaatttc gtctcaaat cgcaacaag tgtaaggac 
      gaggaggag gatattatc cggtgtatt tgtgggatg 
      ccacgcgat gaaggcttg atgatacag tgtgagagg 
      tgcatggtg tggcagcat agcgagtgt gtgaaagcg 
      gacgtttcg gccgaaagt tatcactgt gagcagtgt 
      gtgcccagg gaggttgac tacgagata cctctggat 
      gaattcaca gaacatggg catagatat tacatgacg 
      ctgttgcga ggcgaattg cagctaaga caaggagac 
      acggtctat gttttacgt gatattcca attcctggg 
      acggatcgg aaacacact tatgacact attgggaaa 
      attgaatat tccgaattg gatatattt aggattgaa 
      cgcttgtgg aaagattcc aagactggg caacgcttt 
      gcttatggt caccattat ttacgacca catgagacc 
      taccatgaa ccgaccaga aagtttttc cccaacgaa 
      gtgatgcgg gtgccgctg tacgaggcc gtgcccgtc 
      gagctgata atctcgcat tgttgggtg atggacgtg 
      aacacgttt tgcaagggg cggcccatt ggcgccccc 
      gaagagcac atctacatc tgcgaatat cgcgttgat 
      aaatcggcg cgtttgttt agcaaagtc tcaaaatca 
      aaattcccg atttgtacc aaaagtttc gctttcgaa 
      cgttttgaa acccgactt aaagtctcc cggacgtac 
      actccgcac gatttggac ccggcgtta gtgaaaccg 
      cgaggtcgc aaacctcaa gaaaccgaa gatagagga 
      gaaactgtc attccacca cctctgcca gtgcccgtg 
      cctgtcata agaactcct gtggagcaa aaagctcgt 
      ttgaataaa attctgttg aatctcttg agcaaaatg 
      ccaacgaag caagcgttg gacgtgtcg tatcttctc 
      gaaggaggg aggagaagg aagaagcca gttgagaat 
      ggtaaaaat taa
    mRNA sequence
    • >TC032222 RA
      caagacgct gttgcgcaa ctgacgttt cgtacatgt 
      aacattttc aggagttta aaaaccgtg taatgtgta 
      aactgaaat aatccaagt tgataagtg attaaatca 
      tctcctgtt gaagaagtg aaacgagac aaaagtgcc 
      aaccatgaa aatctccgc atacgcgac ctgaaatca 
      gctggatgc catcttgtt tgtaaagtg gaacataaa 
      catggaaat agatttttt tagcaacga ttaatgtga 
      aaaaatggc aggaaatga agcaaccag ccaaacaga 
      attatggtc ttcggtggt ccaccaacc atgcgagga 
      gcccgagga gtcagaatt gcccagtac ggtggacat 
      ggccgcttt cagtaccgg actaaccgt agaacccgc 
      caacacatc gcaaaataa tgagaattc cgacagtga 
      cagcgactc agaacccga atctagtaa ttcaagtga 
      agcttgcca atcttccag ccagagcga ctcttctga 
      ttctgacag ttcctcgtc cagtgttga atcatcgtc 
      aaggagccc ctcgccgga attttcagt caccacttc 
      ccaaaccaa cagtttaag gctcacaat tgccactgt 
      aaggaagtc atcaggaag tccaccggt tgataaaat 
      gaccaaacc ggctgactc taaaccggc gaaaagtgg 
      tgactgcag ctcctcgtc agactcagt gtgttcttc 
      tgattccga aagcgattc ggaagcttc cggtgctaa 
      aagttcagt taagacgag tgtgaaaag tgccaagga 
      taataatgt taggagaag tgccgcgag actgcgaac 
      tagagtgcc tgataagaa agacgaaaa gaaggccaa 
      gaaggaggc ggcgcccgc caagggccc cgtcaccag 
      gcctaggac caggcaaag gaagaccaa aaagatggc 
      ggcatcgtt gcctctatc ccgtggctc gacctattc 
      cagctcgga ctcggagga ttcggacgc cgatagggc 
      cattctagc ctcctgcag tttgcagga aatacgcca 
      ggaggatct agcagcgat tttacccga ccagcagga 
      atgcgacgc cttcggcga ctttgaatg taacagggt 
      tgataaaaa ttcaccaca aaccggtga catgtccag 
      ctctgacat ggagctgcc acagcaagc ggtcaatgc 
      cctcataca gagaactac agaaagttc cagcgaatc 
      cgaatcgca tgcaccccc taatccctc aactcttta 
      tgccaacag tttgctaca acaattcgt cgctcaaac 
      ccaaatgct aaatgcacc gtgtcctgt aataccgac 
      agggaccga tggcattaa aaccctacc ccttaacac 
      ttgcgaaaa tcaaagtaa taacaataa taccacgaa 
      taataataa taacaacac aggaggtga tacggttaa 
      aaggaaaag aggacggcc gagaaaaaa tcacaagac 
      acaaaatga taacaaaac tcaaaataa aacaattga 
      gtattgtgg tgatcctaa cgtttcacc cgattccgg 
      gattcagaa tagtcccga ccacgtctc aagtcccga 
      accaaccct aactttaaa aaacaaaga agagaataa 
      aaaagacaa caaaacaaa aactgtcaa taaatcgaa 
      agaaataaa caaattatc agtgacttc gaatcggtt 
      cgatcgctt gttgtatgc taacaccga ccgggttct 
      ctacccgcc ccgaaggaa agtaggccg gcctccgtt 
      aacaaaaaa aggcccagg taggccccc caaatacaa 
      accaccagc caacacaaa ccaagaaac aaaaccgcc 
      cgataaaat taaaccaaa aaccgaacc acggcccgt 
      acttgtaac caaaaacaa aatcgacaa taccaaagt 
      gaaattcac aacattgaa aaatctcaa agtgatgca 
      cagcaaaca caaacacaa gaaacacaa aaaatacaa 
      aatcaaaat cctcaaacc attgaccgg aaccaacga 
      caaagtcgt gatcgaaat cgataaact agtgtcaga 
      ctttacgaa attttgcgt gattggaag tacgaaacc 
      gccgaaaga gaacgtacc cgaagtgaa accggcgaa 
      gaaagtgtc gaaaaagcg caaaaccac ggaacagaa 
      gaagaagaa gattagtgt gaatgccac cgttaataa 
      agtggcgaa tttaaacga gcagagatt gccgttaaa 
      aaagcgaca ttacttgta ttccgatgg caagaaaag 
      cgaggagat tctcccgaa agaggagaa agtcagtgc 
      gaataataa ttccataac ggcgaccac gccgaagaa 
      gcgacaccg actggaggt gatcaacaa ggagaagga 
      aaatgcggc cgttccggc ggtcgagcc taaagtcaa 
      gcccaaact gtctctaga atccctcat ttccgaatt 
      gaagatgaa gagagggtt gtcaggtca ggaggacag 
      tttggacgc ggtcaaagt cgactcggt tgtcagtcc 
      ggacgctaa aactaaaac caccgttga gaataattt 
      gaagaagaa gccgcggaa gcggcgcgc catcaaccg 
      gaccgggtt ccccactgt caagaagaa gaagaagaa 
      ggtggtttc ggtggaaga ttcggtcga taccgagga 
      agataagcc tttgattag ctgtgatcg cgtcccgaa 
      agacggcga agagtacaa taaattcat tcaaaggac 
      ggaaaatgc cgacgcgtc caaaaccga cgattccct 
      atcgaagtg ggaagtgat gagcgagtg tgatagtct 
      cccgcagga tgaacgcat cgaggataa gggggacag 
      tggcgggga catggacga ggtctcact tgaggcgag 
      cattgaacg gttgaggtc acgacttga taaaaagaa 
      gcggaaacg tgcggaaaa aatcgtcga tttgaacgg 
      ttatcccaa aaggcggct tagggacat atccccagc 
      tagtagtat tgatgttat catcgagcg ccgcttacg 
      cgaggaacg agcttccgc ctccggcga cgaaatgaa 
      gaagaacaa gaaggcgcc aaggtggcg caagcgcta 
      cctggttgc tggtctttt ctccgatta tttcaaaga 
      ggacgacga gacttgtag gattaaaag gagcgaaaa 
      cgcggcgaa gtcccgcct ggtgtacaa cccggccga 
      gcatccgta cggtctttt accgccgcc ctatcactg 
      cggtaaata tttacggtg tcggaagct tcccttcca 
      gttaccgta cgacttgtg gtggcaaca tacgcattc 
      gcagttgcc aggcaggga tgttgtacc gtcttggaa 
      ttatcggaa aatacggac gaatgttta taatgttaa 
      gacgacgac gggagcgtg cgaaccaca aacttgcaa 
      ttgtacgcc gttggctaa ttgtggtga cgattgtat 
      taatcgact tgtgttggc cgaatgtcc cgccagcca 
      caagtgcca aaaccagaa gattcagag gcatgagtg 
      ggcgccggg gcttgagaa attcatgac cgaaaataa 
      gggttgggg cgtgcgcac gaaactccc catcaagtc 
      cggggaatt catcctgga gtacgtggg cgaggtggt 
      gtccgacca ggaattcaa agaacgcat ggctaccat 
      ttacgtcaa cgacaccca ccactactg cttgcacct 
      agacggtgg tttggtcat cgacggcca ccgaatggg 
      cggtgacgg ccgtttcgt caatcattc ctgtcaacc 
      aaactgcga gatgcagaa atggtcagt caacggcca 
      gttccgaat ggctttatt cgccttgcg cgacattga 
      aagttccga agaactaac atacgacta caacttctc 
      acttttcaa tccggccga aggccagga atgcaaatg 
      cggcagcga aatgtgccg tggcgtcat cggaggcaa 
      gtcgcaaag agtgcgcca actgccgga agtcaaaga 
      cgggaaaaa tccaggacg tgtgggacg accgcgaaa 
      aaatcaagc caagcggac tacaagtgt taaagatct 
      gccattggc gcagccaag cgtgccgat agtggcgcc 
      cccgcaagt caagccgat gagtcacca acagaaatg 
      tttcattct ggagcacca ctgtttcct cttacggaa 
      cctaacgaa ggtgaaacg ggtgcggga taggtcgag 
      cagttcgct catttcgcg ccaacaagc catgacgcc 
      gaccaatca aggcgctgc gtttatcaa tcatttgaa 
      cgcgttggg aaaaccgcg gaatattcg gaccaggcg 
      attggccca agccgagga caatcccga gttgagtaa 
      aacagtgaa acttgccgc cgtgttacg ggagatttg 
      gaacacagt gacgaaggc caaagacga gaataacga 
      atcgttgag tacgcattt tatgacaat gccatcgaa 
      acgcaaagt acctgagtt ctatcaacg cattactaa 
      tccgattga tctcgccac catcgagca aaacatcgc 
      cactggcta ttacctggc gcctgaaag cttcaattc 
      ggatatgaa tcgaatgtt tgccaatta tttgcagtt 
      ttacgggcg gacgaagga aatcggcgt tgctgcgat 
      taaattgaa gaaagtcta cgtcgatgc caaacaaca 
      aagtttgcc caaattcga aaacgtgtt aggaaaaaa 
      accaccgaa tttcgtctc aaatcgcaa caagtgtaa 
      ggacgagga ggaggatat tatccggtg tatttgtgg 
      gatgccacg cgatgaagg cttgatgat acagtgtga 
      gaggtgcat ggtgtggca gcatagcga gtgtgtgaa 
      agcggacgt ttcggccga aagttatca ctgtgagca 
      gtgtgtgcc cagggaggt tgactacga gatacctct 
      ggatgaatt cacagaaca tgggcatag atattacat 
      gacgctgtt gcgaggcga attgcagct aagacaagg 
      agacacggt ctatgtttt acgtgatat tccaattcc 
      tgggacgga tcggaaaca cacttatga cactattgg 
      gaaaattga atattccga attggatat atttaggat 
      tgaacgctt gtggaaaga ttccaagac tgggcaacg 
      ctttgctta tggtcacca ttatttacg accacatga 
      gacctacca tgaaccgac cagaaagtt tttccccaa 
      cgaagtgat gcgggtgcc gctgtacga ggccgtgcc 
      cgtcgagct gataatctc gcattgttg ggtgatgga 
      cgtgaacac gttttgcaa ggggcggcc cattggcgc 
      ccccgaaga gcacatcta catctgcga atatcgcgt 
      tgataaatc ggcgcgttt gtttagcaa agtctcaaa 
      atcaaaatt cccgatttg taccaaaag tttcgcttt 
      cgaacgttt tgaaacccg acttaaagt ctcccggac 
      gtacactcc gcacgattt ggacccggc gttagtgaa 
      accgcgagg tcgcaaacc tcaagaaac cgaagatag 
      aggagaaac tgtcattcc accacctct gccagtgcc 
      cgtgcctgt cataagaac tcctgtgga gcaaaaagc 
      tcgtttgaa taaaattct gttgaatct cttgagcaa 
      aatgccaac gaagcaagc gttggacgt gtcgtatct 
      tctcgaagg agggaggag aaggaagaa gccagttga 
      gaatggtaa aaattaatg aaatgggtg atattaaga 
      agtaactct tatttttta tgcgacgct attgaatgt 
      tatttatct ttattgcta gggctaatg gtaggtttg 
      tttgtaaat gtttctcat aggtcttaa cgactagtc 
      atagttctg ttgttaatt ttgtctttt tcattattt 
      atttgtaca taatgaaat tgttgcctc taagtatgt 
      tgcaagttg gagagagat taacgaatt ggaaattta 
      aggtactat taatttttt gtctcaatc ccagtgtac 
      aaatttaat ttttcagtc atttggtta gaaacgtag 
      gttttcagc gctgttgct tattccacg tcggactta 
      atttatttt attttcttt tacttatag aattcttgc 
      ctgattaga tcataaggt ttaacatta atacatgtt 
      tttgaacat t